Glutelin, the major protein fraction from guava seed, was obtained by fractioning as described by Osborne. The total proteins
were extracted and the isolates obtained by isoelectric precipitation presented similar DSC curves, concordant with the results
obtained by gel filtration chromatography and electrophoresis in polyacrylamide gel (PAGE-SDS). However, the DSC curves showed
a higher enthalpy with regard to the denaturing protein isolate (PI) extracted at pH 10.0 when compared to a PI at pH 11.5.
Such results are in accordance with those obtained for PI extracted at pH 10.0 using chromatography, this one being present
in the form of molecular aggregates of greater molecular mass. The glutelin fraction, however, did not present a denaturation
peak in the DSC curve, showing that the process for obtaining the same significantly altered its conformation.