Journal of Thermal Analysis and Calorimetry
Volume/Issue:
Volume 95: Issue 3
Effect of sucrose on BSA denatured aggregation at high concentration studied by the iso-conversional method and the master plots method
Authors: X. Cao 1 , Z. Wang 1 , X. Yang 1 , Y. Liu, and C. Wang 1
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  • 1 Wuhan University College of Chemistry and Molecular Science Wuhan 430072, Hubei China
  • 2 Wuhan University College of Life Science Wuhan 430072, Hubei China
DOI:
https://doi.org/10.1007/s10973-008-9307-5
Page Count:
969–976
Publication Date:
01 Mar 2009
Online Publication Date:
05 Feb 2009
Article Category:
Research Article
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Abstract  

The kinetics of protein thermal transition is of a significant interest from the standpoint of medical treatment. The effect of sucrose (0–15 mass%) on bovine serum albumin denatured aggregation kinetics at high concentration was studied by the iso-conversional method and the master plots method using differential scanning calorimetry. The observed aggregation was irreversible and conformed to the simple order reaction. The denaturation temperature (Tm), the kinetic triplets all increased as the sucrose concentration increased, which indicated the remarkable stabilization effect of sucrose. The study purpose is to provide new opportunities in exploring aggregation kinetics mechanisms in the presence of additive.

Cover Journal of Thermal Analysis and Calorimetry
Journal of Thermal Analysis and Calorimetry
Print ISSN:
1388-6150
Online ISSN:
1572-8943
Keywords:
denatured aggregation; DSC; iso-conversional method; master plots method

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