Univ. Nacional de La Plata Centro de Investigación y Desarrollo en Ciencias Aplicadas-Dr. Jorge J. Ronco, Centro Científico Tecnológico La Plata, CONICET Calle 47 Nro. 257 B1900AJK La Plata, Buenos Aires Argentina
The stability of Novozym® 435 in the esterification of ibuprofen using ethanol as reactant and solvent was investigated. Additionally, the surface
interaction of the isomers of ibuprofen and ethanol with the biocatalyst was screened through conventional adsorption isotherms
and temperature programmed surface reaction (TPSR). These investigations evidenced strong alcohol adsorption and dissolution
of the biocatalyst, which explains its deactivation upon reuses in the esterification reaction.