Interdisciplinary synthesis and validity analysis (ISVA), a structured learning approach which integrates learning and communication theories, meta-analytic evaluation methods, and literature management-related technologies was applied in the context of the 1993–1997 bovine mastitis research literature. This study investigated whether ISVA could: 1) facilitate the analysis and synthesis of interdisciplinary knowledge claims, and 2) generate projects or research questions. The bovine mastitis-related literature was conceptualized as composed of microbiological, immunological, and epidemiological dimensions. Keywords involving these dimensions were searched in theMedline andAgricola databases. A final list of 148 articles were retrieved, analyzed, synthesized into fifteen information sub-sets, and evaluated for construct, internal, external and statistical validity through an interdisciplinary iterative dialogical process. Validity threats were re-phrased as new research or educational projects.