View More View Less
  • 1 School of Pharmacy, Nanjing Medical University, Nanjing City, 210029, P.R. China
Restricted access

Summary

A reversed-phase chiral liquid chromatographic method had been developed and validated for resolution of the enantiomers of racemic fudosteine. The effects on the separation of the amounts of anhydrous cupric sulfate and l-phenylalanine, the methanol content, mobile phase pH, and temperature were investigated. The method was validated for linearity, repeatability, intermediate precision, sample recovery, solution stability, and limits of detection (LOD). l-Phenylalanine and anhydrous cupric sulfate as chiral ligand-exchange complexes were used for separation, isomer identification, related substance investigation, and analysis of fudosteine enantiomers in fudosteine bulk drugs and fudosteine tablets.

  • [1]. H. Komatsu S. Yamaguchi N. Komorita K. Goto S. Takagi H. Ochi T. Okumoto 2005 Pulm. Pharmacol. Ther. 18 121.

  • [2]. L. Li X.Y. Chen X.J. Dai H. Chen D.F. Zhong 2007 J. Chromatogr. B 856 171.

  • [3]. J. Wen Y.W. Wu L.L. Zhang Y.P. Qi G.R. Fan Y.T. Wu Z. Li 2008 J. Chromatogr. B 867 153.

  • [4]. S. Kumari S. Prabhakar M. Vairamani C.L. Devi G.K. Chaitanya K. Bhanuprakash 2007 J. Am. Soc. Mass Spectrom. 18 1516.

  • [5]. L. Strekowski M.T. Cegla V. Honkan H. Buczak W.R. Winkeljohn A.L. Baumstark W.D. Wilson 2005 Bioorg. Med. Chem. Lett. 15 2720.

  • [6]. G.S. Yang S.L. Yuan X.J. Lin Z.N. Qi C.B. Liu H.Y. Aboul-Enein G. Félix 2004 Talanta 64 320.

  • [7]. Y.J. Choi S. Jung 2004 Carbohydr. Res. 339 1961.

  • [8]. Z. Mustapha B. Isabelle R. Corinne H. Somnath H. Mohamed F. Jennifer D. Jean-Luc P. Eric 2008 J. Chromatogr. A 1185 291.

  • [9]. G.J. Ma B.L. Gong C. Yan 2008 Chin. J. Anal. Chem. 36 275.

  • [10]. H.I. Wen S. Huang W.Y. Liu 2004 Chin. J. New Drugs 13 1135.

  • [11]. M. Shen 2005 Qilu. Pharm. Affai. 24 464.

  • [12]. X.L. Song W. Zhang 2005 Chin. J. Pharm. Anal. 25 1143.

  • [13]. Q.J. Lin Z.M. Chen 2007 Chin. J. Pharm. 38 872.

  • [14]. G.M. Ran L. Yang L.Q. Yan K. Liu X.J. Guo 2005 Chin. J. Pharm. Anal. 25 1561.

  • [15]. Y. Wang T.J. Hang 2005 Pro. Pharm. Sci. 29 421.

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Nov 2020 0 3 1
Dec 2020 0 0 0
Jan 2021 5 0 0
Feb 2021 5 0 0
Mar 2021 5 0 0
Apr 2021 2 0 0
May 2021 0 0 0