A new, simplified open-vessel microwave extraction (OVME) method with solid-phase extraction (SPE) for sample clean-up has been developed for specific investigation of sennosides A and B. Aqueous extracts of senna leaves (Senna folium) were prepared by different methods, including classic pharmacopoeial methods: concoction, infusion, and ultrasound extractions. The different extraction methods were compared by analysis of the sennosides A and B in the samples with a modified enantiomer-selective reversed-phase high-performance liquid chromatographic-tandem mass spectrometric method. HPLC was performed with a 4.6 mm × 150 mm, 3.5-µm particle C8 column and simple gradient elution. The benefit of open-vessel microwave extraction is discussed.
[1]. S. Başgel S.B. Erdemoğlu 2006 Sci. Total. Environ. 359 82–89.
[2]. E. Ernst 1998 Am. J. Med. 104 171–178.
[3]. European Medicines Agency: Community Herbal Monograph on Cassia Senna L. and Cassia Angustifolia Vahl, Folium. London, 26 October 2006. http://www.emea.europa.eu/pdfs/human/hmpc/sennae_folium/5186906en.pdf.
[4]. V. Lopez-Avila 1999 Crit. Rev. Anal. Chem. 29 195–230.
[5]. V. Camel 2000 Trends Anal. Chem. 19 229–248.
[6]. M. Letellier H. Budzinski L. Charrier S. Wise 1996 Spectroscopy 13 71–80.
[7]. M. Letellier H. Budzinski 1999 Analysis 27 259–271.
[8]. Á.Z. Dávid , G. Rajkai, K. Ludányi, M. Lengyel, I. Antal, and I. Klebovich, Evaluation of Microwave-Assisted Extraction for the Preparation of Medical Teas, 5th World Meeting on Pharmaceutics, Biopharmaceutics, and Pharmaceutical Technology, Geneva, March 27–30, 2006; poster #87, program book p. 64.
[9]. C.S. Eskilsson E. Björklund 2000 J. Chromatogr. A 902 227–250.
[10]. J. Koyama I. Morita N. Kobayashi 2007 J. Chromatogr. A 1145 183–189.
[11]. Y. Min H. Jian C. Hubiao Z. Junhua G. Dean 2007 J. Am. Soc. Mass. Spectrom. 18 82–91.
[12]. M.R.S. Fuh H.J. Lin 2003 Tamkang J. Sci. Eng. 6 31–36.
[13]. A. Arshi M.Z. Abdin M. Iqbal 2006 Sci. Hortic. — Amsterdam 111 84–90.
[14]. C. Terreaux Q. Wang J.R. Ioset K. Ndjoko W. Grimminger K. Hostettmann 2002 Planta Med. 68 349–354.
[15]. T. Padumanonda W. Gritsanapan 2006 Southeast Asian J. Trop. Med. Public Health 37 388–393.
[16]. J. Koyama I. Morita N. Kobayashi 2007 J. Chromatogr. A 1145 183–189.
[17]. S.W. Sun H.T. Su 2002 J. Pharm. Biomed. Anal. 29 881–894.
[18]. S. Zhao K.C. Kwok H. Liang 2007 Sep. Purif. Technol. 55 307–312.