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  • 1 Natural Products Chemistry Division, Indian Institute of Integrative Medicine (CSIR), Canal Road, Jammu Tawi, 180001, India
  • 2 Pharmacology Division, Indian Institute of Integrative Medicine (CSIR), Canal Road, Jammu Tawi, 180001, India
  • 3 Plant Survey Division, Indian Institute of Integrative Medicine (CSIR), Canal Road, Jammu Tawi, 180001, India
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Summary

In this paper we describe a sensitive and reproducible reversed-phase high-performance liquid chromatography (HPLC) method with photodiode-array detection for isolation and quantification of the bioactive hydrophilic constituent 7-(1-O-β-d-galacturonide-4′-(1-O-β-d-glucopyranosyl)-3′,4′,5,7-tetrahydroxyflavone, 1, from the seeds of Cuminum cyminum. Compound 1 was separated isocratically on a C18 preparative column, in high purity, after removal of solvents. The purity and identity of the compound were established by use of LC-mass spectrometry and by spectroscopic techniques (1H and 13C NMR). The purity of 1 was also confirmed by HPTLC.

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