View More View Less
  • 1 Tulip Lab Pvt. Ltd. F-20/21, MIDC Ranjangaon, Tal-Shirur, Pune, 412220, India
  • | 2 Marathwada Mitra Mandal College of Pharmacy, Pune, India
  • | 3 Natural Remedies, Bangalore, India
Restricted access

Cross Mark

Summary

This paper describes a new, simple, precise, and accurate HPTLC method for quantification of (−)-epicatechin in the leaves of Cassia fistula. The leaves were separately extracted with methanol and water by both maceration and hot extraction (Soxhlet apparatus). Chromatographic separation of the drug was performed on aluminium foil silica gel 60 F254 plates with toluene-ethyl acetate-formic acid-methanol 20:12:4:4 (v/v) as mobile phase. Densitometric evaluation of the separated zone was performed at 280 nm. Epicatechin in the extract was satisfactorily resolved with RF 0.22 ± 0.02. The accuracy and reliability of the method were assessed by evaluation of linearity (200–800 ng per band), precision (method precision RSD 1.42% and instrumental precision RSD 1.12%), accuracy (98.12%), and specificity in accordance with ICH guidelines.

  • [1]. Anonymous 1992 The Wealth of India, Vol. 3 Publication and information Directorate (CSIR) New Delhi 337343.

  • [2]. K.R. Kirtikar B.A. Basu 1991 Indian Medicinal Plants, Vol. 2 Periodical Experts Book Agency New Delhi 856860.

  • [3]. R. Ilavarasan M. Mallika S. Venkataraman 2005 Afr. J. Trad. CAM. 2 70.

  • [4]. T. Bhakta P.K. Mukherjee M. Pal B.P. Saha 1998 Pharm. Biol. 36 140.

  • [5]. T. Bhakta P.K. Mukherjee M. Pal B.P. Saha 1998 Nat. Prod. Sci. 4 84.

  • [6]. V. Narayanan T. R. Seshadri 1972 Indian J. Chem. 10 379.

  • [7]. Y.H. Kuo P.H. Lee Y.S. Wein 2002 J. Nat. Prod. 65 1165.

  • [8]. C.K. Lee Y.Y. Chung F.L. Hsu Y.H. Kuo 2003 Chin. Pharm. J. 55 231.

  • [9]. B. Theeshan S.N. Vidushi I.A. Okezie 2005 Afr. J. Biotechnol. 13 1530.

  • [10]. ICH Q2B. Harmonized Tripartite Guideline, Validation of Analytical Procedures: Methodology, IFPMA. In: Proc. Int. Conf. Harmonization, Geneva, 1996.

    • Search Google Scholar
    • Export Citation
All Time Past Year Past 30 Days
Abstract Views 8 8 1
Full Text Views 9 7 0
PDF Downloads 4 4 0