View More View Less
  • 1 Department of Applied Chemistry, Yantai University, Yantai, 264005, P.R. China
Restricted access

Summary

A simple and rapid capillary electrophoretic procedure for analysis of matrine and oxymatrine in Kushen medicinal preparations has been developed and optimized. Orthogonal design was used to optimize the separation and detection conditions for the two active components. Phosphate concentration, applied potential, organic modifier content, and buffer pH were selected as variable conditions. The optimized background electrolyte contained 70 mM sodium dihydrogen phosphate and 30% acetonitrile at pH 5.5; the separation potential was 20 kV. Each analysis was complete within 5 min. Regression equations revealed linear relationships (r > 0.999) between peak area and amount for each component. The detection limits were 1.29 μg mL−1 for matrine and 1.48 μg mL−1 for oxymatrine. The levels of the two active compounds in two kinds of traditional Chinese medicinal preparation were easily determined with recoveries of 96.57–106.26%. In addition, multiple linear regression and a non-linear model using a radial basis function neural network approach were constructed for prediction of the migration time of oxymatrine. The predicted results were in good agreement with the experimental values, indicating that a radial basis function neural network is a potential means of prediction of separation time in capillary electrophoresis.

  • [1]. Y.Q. Wang Y.Y. Ma X.H. Li F. Qin X.M. Lu F.M. Li 2007 Biomed. Chromatogr. 21 876.

  • [2]. H.L. Sun L. Li L. Shang D. Zhao D.L. Dong G.F. Qiao Y. Liu W.F. Chu B.F. Yang 2008 Phytother. Res. 22 985.

  • [3]. H.C. Jiang F.Q. Meng J. Li X.Y. Sun 2005 World J. Surg. 29 1397.

  • [4]. X.Y. Liu H. Fang Z.G. Yang X.Y. Wang L.M. Ruan D.R. Fang Y.G. Ding Y.N. Wang Y. Zhang X.L. Jiang H.C. Chen 2008 Int. J. Dermatol. 47 448.

    • Search Google Scholar
    • Export Citation
  • [5]. L.D. Ma S.H. Wen Y. Zhan Y.J. He X.S. Liu J.K. Jiang 2008 Planta Med. 74 245.

  • [6]. F.Q. Yang J. Quan T.Y. Zhang Y. Ito 1998 J. Chromatogr., A 822 316.

  • [7]. X.H. Liu L. Li J. Sun Y.H. Sun T.H. Zhang D.W. Chen Z.G. He 2006 Chromatographia 63 483.

  • [8]. D.S.T. Sit G.H. Gao F.C.P. Law P.C.H. Li 2004 J. Chromatogr. B 808 209.

  • [9]. P.C.H. Li G.H. Gao F.C.P. Law 2004 J. Liq. Chromatogr. Relat. Technol. 27 2861.

  • [10]. Y.Q. Yu P.L. Ding D.F. Chen 2004 Anal. Chim. Acta 523 15.

  • [11]. X. Wang W. Zhang L.Y. Fan B. Hao A.N. Ma C.X. Cao Y.X. Wang 2007 Anal. Chim. Acta 594 290.

  • [12]. S. Li S. Wang 1999 China J. Chin. Materia Medica 24 100.

  • [13]. S. Liu Q. Li X. Chen Z. Hu 2002 Electrophoresis 23 3392.

  • [14]. G.Q. Zhang L. L. Zhao 2008 Academic Journal of Second Military Medical University 29 108.

  • [15]. Y.R. Ku L.Y. Chang J.H. Lin L.K. Ho 2002 J. Pharm. Biomed. Anal. 28 1005.

  • [16]. Y. Sun T. Guo Y. Sui F.M. Li 2003 J. Chromatogr. B 792 147.

  • [17]. G.X. Sun Y. Wang Y.Q. Sun K.S. Bi 2003 Anal. Sci. 19 1395.

  • [18]. Y.B. Ji Y.Y. Chen R.J. Wu 2003 Chin. Trad. Herbal Drugs 34 1127.

  • [19]. A.C. Servais M. Fillet P. Chiap A.M. Abushoffa P. Hubert J. Crommen 2002 J. Sep. Sci. 25 1087.

  • [20]. S. Toasakairi D.L. Massart Y.V. Heyden 2000 Anal. Chim. Acta 416 29.

  • [21]. Y. Daali S. Cherkaoui P. Christen J.L. Veuthey 1999 Electrophoresis 20 3424.

  • [22]. Y.P. Zhang X.J. Li Z.B. Yuan Y.Q. Lu 2002 Microchem. J. 73 307.

  • [23]. Z.L. Liu Y. Yu P.N. Shen J. Wang C.Y. Wang Y.J. Shen 2008 Sep. Purif. Technol. 58 343.

  • [24]. Y.Q. Cheng H.L. Chen L.Y. Fan X.G. Chen Z.D. Hu 2004 Anal. Chim. Acta 525 239.

  • [25]. F.J. Garci˘ia-March G.M. Anton-Fos F. Perez-Gimenez M.T. Salaberti-Salvador R.A. Cercos-del-Pozo J.V. de Julian-Ortiz 1996 J. Chromatogr. A 719 45.

    • Search Google Scholar
    • Export Citation
  • [26]. T.F. Woloszyn P.C. Jurs 1992 Anal. Chem. (Washington, DC, U. S.) 64 3059.

  • [27]. J. Arning S. Stolte A. Boschen F. Stock W.R. Pitner U.W. Biermann B. Jastorff J. Ranke 2008 Green Chem. 10 47.

  • [28]. A.B. Hameda S. Elosta J. Havel 2005 J. Chromatogr. A 1084 7.

  • [29]. E. Baher M.H. Fatemi E. Konoz H. Golmohammadi 2007 Microchim Acta 158 117.

  • [30]. P. Polaskova G. Bocaz H. Li J. Havel 2002 J. Chromatogr. A 979 59.

  • [31]. S. Oszwałdowski A.R. Timerbaev 2007 J. Chromatogr. A 1146 258.

  • [32]. H.T. Liu Y.Y. Wen F. Luan Y. Gao 2009 Anal. Chim. Acta 638 88.

  • [33]. E. Deponinck D. Coomans Y.V. Heyden 2007 J. Pharm. Biomed. Anal. 43 119.

  • [34]. D.L. Massart B.G.M. Vandeginste L.M.C. Buydens S. D. Jong P.J. Lewi J. Smeyers-Verbeke 1997 Handbook of Chemometrics and Qualimetrics-Part A Elsevier Science Amsterdam.

    • Search Google Scholar
    • Export Citation
  • [35]. F. Luan H.T. Liu Y.Y. Wen X.Y. Zhang 2008 Anal. Chim. Acta 612 126.

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Oct 2020 0 0 0
Nov 2020 0 6 1
Dec 2020 0 0 0
Jan 2021 0 0 0
Feb 2021 0 0 0
Mar 2021 1 0 0
Apr 2021 1 0 0