View More View Less
  • 1 Faculty of Pharmacy, University of Beni-Sueif, Beni-Sueif, Egypt
  • 2 H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan
Restricted access

Summary

Ashwaghanda, Withania somnifera, is one of the most widely used herbs in Ayurvedic medicine. Leaves and roots are the traditionally used parts of the plant. An RP-HPLC method using a binary acetonitrile-water gradient containing 0.1% acetic acid has been developed for analysis of withaferin A. The method was validated in accordance with ICH guidelines and used for analysis of the withanolide content of the flowers, leaves, and roots of W. somnifera. The withanolide content was highest in the flowers.

  • [1]. R. Schliebs S.K. Liebmann A. Kumar S. Ghosal V. Bigl 1973 Neurochem. Int. 30 181.

  • [2]. A.B. Ray M. Gupta 1994 Prog. Chem. Org. Nat. Prod. 63 2.

  • [3]. S.K. Bhattacharya K.S. Satyan S. Ghosal 1997 Indian J. Exp. Biol. 35 236.

  • [4]. S.K. Kulkarni B. George R. Mathur 1998 Phytother. Res. 12 451.

  • [5]. J.N. Dhuley 2001 Phytother. Res. 15 311.

  • [6]. A.D. Kinghorn B.N. Su D.S. Jang L.C. Chang D. Lee J.Q. Gu E.J. Carcache-Blanco A.C. Pawlus S.K. Lee E.J. Park M. Cuedei J.J. Gills K. Bhat H.S. Park E. Mata-Greenwod L.L. Song M. Jang J.M. Pessuto 2004 Planta Med. 70 691.

    • Search Google Scholar
    • Export Citation
  • [7]. C. Tohda K. Komaatuse T. Kuboyama 2005 J. Tradit. Med. 22 176.

  • [8]. H. Ichikawa Y. Takada S. Shishodia B. Jayaprakasam M.G. Nair B.B. Aggrawal 2006 Mol. Cancer Ther. 5 1434.

  • [9]. M. Kaileh W.V. Berghe A. Heyerick J. Horion J. Plette C. Libert D. De Keukelerie T. Essawi G. Haegeman 2007 J. Biol. Chem. 282 4253.

    • Search Google Scholar
    • Export Citation
  • [10]. R.S. Sangwan , N.D. Chaurasiya, L.N. Misra, P. Lal, G.C. Uniyal, N.S. Sangwan, A.K. Srivastava, K.A. Suri, G.N. Qazi, and R. Tuli, US Patent # 7, 108, 870 (2005).

  • [11]. N.D. Chaurasiya G.C. Uniyal P. Lal L. Misra N.S. Sangwan R. Tuli R.S. Sangwa 2008 Phytochem. Anal. 19 148.

  • [12]. S. Dalavayi S.M. Kulkarni R.L. Itikala S. Itikala 2006 Ind. J. Pharm. Sci. 68 253.

  • [13]. M. Ganzera M.I. Choudhary I.A. Khan 2003 Fitoterapia 74 68.

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Oct 2020 5 0 0
Nov 2020 2 0 0
Dec 2020 9 0 0
Jan 2021 2 0 0
Feb 2021 4 0 0
Mar 2021 6 0 0
Apr 2021 9 0 0