View More View Less
  • 1 Department of Chemistry, Lanzhou University, Lanzhou, 730000, China
  • | 2 Geological Scientific Research Institute of Shengli Oilfield, Sinopec, Dongying, 257001, China
Restricted access

Summary

1,7-Dihydroxy-3,8-dimethoxyxanthone (X1) and 1,8-dihydroxy-3,7-dimethoxyxanthone (X2) are two important xanthones of the Tibetan medicinal plant Gentianopsis paludosa (Hook. f.) Ma. They are very similar in structure, the only difference being exchange of OH and OCH3 at the 7 and 8 positions. By calculations based on the geometry of the molecules using the MM+ force field, the different distances between the hydroxyl groups of the two xanthones were obtained (4.64774 Å for X2 and 7.19412 Å for X1), therefore, the two hydroxyl groups of X1 should freely interact with more water molecules than those of X2 in aqueous solution. In other words, X2 is more hydrophobic than X1. Micellar electrokinetic capillary chromatography (MEKC) was therefore chosen for separation of the compounds. The optimum separation conditions were: 20 mm borate + 20 mm SDS (pH 9.8) as running buffer, 17.5 kV applied potential, and detection wavelength 260 nm. The two xanthones were well separated in 9.0 min, with Gaussian peak shapes. The repeatability of the MEKC method (expressed as RSD) for X1 and X2 was 0.9 and 1.1%, respectively, for migration time, and 3.1 and 1.4% for peak area. The limits of detection (S/N = 3) were 0.41 μg mL−1 for X1 and 0.82 μg mL−1 for X2. The recovery of the MEKC method for the two xanthones was also satisfactory.

  • [1]. T.N. He 1988 Flora of China Science Press Beijing 294297.

  • [2]. B.Z. Guo 1987 Medicinal Plants of Qinghai Province Qinghai People's Publishing House Xining 458.

  • [3]. Z.P. Li C.H. Huo X.L. Zheng 2003 Chin. J. Mod. Appl. Pharm. 20 24.

  • [4]. H.D. Wang C.Y. Tan Y.G. Du X.F. Bai H.F. Lu 2004 Chin. J. Chin. Mater. Med. 29 1055.

  • [5]. H.D. Wang C.Y. Tan Y.G. Du X.F. Bai B.C. Lin 2005 Chin. Pharm. J. 40 1059.

  • [6]. Q. Mi C.N. Cao Z.X. Zhao L.Y. Tu 2005 J. Qinghai Norm. Univ. (Nat. Sci.) 2 64.

  • [7]. H.D. Wang C.Y. Tan X.F. Bai Y.G. Du B.C. Lin 2006 J. Ethnopharmacol. 105 114.

  • [8]. Jiangsu Institute of Botany 1988 Chinese Academy of Medical Sciences and Kunming Institute of Botany, Xinhua Bencao Gangyao Shanghai Science and Technology Press Shanghai 292.

    • Search Google Scholar
    • Export Citation
  • [9]. H.D. Wang C.Y. Tan X.F. Bai Y.G. Du B.C. Lin 2005 Nat. Prod. Res. Dev. 17 598.

  • [10]. Y.Y. Zong C.Y. Xu 1982 Yaoxue Tongbao 17 206.

  • [11]. H.D. Wang C.Y. Tan J.M. Zhao X.F. Bai Y.G. Du B.C. Lin 2007 Chin. J. Pharm. Anal. 27 421.

  • [12]. M. Jing J.W. Li Q. Fan X. Shi 2008 Chin. J. Inform. Trad. Chin. Med. 15 43.

  • [13]. T. Bo Y.F. Huang X.D. Yang K.A. Li H.W. Liu L.Z. Xu 2003 J. Chromatogr. Sci. 41 182.

  • [14]. X.M. Wu S.X. Gong T. Bo Y.P. Liao H.W. Liu 2004 J. Chromatogr. A 1061 217.

  • [15]. T. Bo X.D. Yang K.A. Li L.Z. Xu H.W. Liu 2003 J. Sep. Sci. 26 133136.

  • [16]. T. Bo X.D. Yang F. Liu K.A. Li L.Z. Xiu H.W. Liu 2002 Anal. Chim. Acta 474 37.

  • [17]. Y.F. Yang Z.X. Liao L. Cuo Y. Chen 2003 J. Liq. Chromatogr. Relat. Technol. 26 1219.

All Time Past Year Past 30 Days
Abstract Views 12 12 0
Full Text Views 3 1 0
PDF Downloads 2 1 0