Acta Chromatographica
Volume/Issue:
Volume 23: Issue 1
Quantitative analysis of berberine in Berberis aristata fruits and in a traditional anti-inflammatory unani formulation by use of a validated HPLC method
Authors: Y. T. Kamal 1 , M. Singh 1 , E. T. Tamboli 1 , R. Parveen 2 , and S. Ahmad 1
View More View Less
  • 1 Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Hamdard University, Hamdard Nagar, New Delhi, India, 110062
  • 2 Department of Pharmaceutics, Faculty of Pharmacy, Hamdard University, Hamdard Nagar, New Delhi, India, 110062
DOI:
https://doi.org/10.1556/achrom.21.2013.1.11
Page Count:
157–168
Publication Date:
01 Mar 2011
Online Publication Date:
01 Mar 2011
Article Category:
Research Article
Restricted access

Summary

A validated reversed-phase HPLC method has been developed for quantitative analysis of berberine in Berberis aristata fruits and in a polyherbal formulation. Separation of berberine was achieved on a C18 column with a mobile phase consisting of a 10–80% acetonitrile gradient in 0.05% aqueous orthophosphoric acid. The flow rate was 1 mL min−1. Detection was at 266 nm. A sharp, well defined peak was obtained at a retention time of 10.0 ± 0.4 min. The method was validated in accordance with ICH guidelines for accuracy, precision, robustness, and the limits of detection (LOD) and quantification (LOQ). Results from linear regression analysis were indicative of a good linear relationship (r2 = 0.998 ± 0.0011) in a wide concentration range (5–500 μ g mL−1). LOD and LOQ were 1.5 and 5.3 μg mL−1, respectively. Satisfactory recovery results (94.6–103.1%) were obtained by the method of standard addition. Intra-day, inter-day, and intersystem precision was satisfactory, with relative standard deviation in the range 0.7–1.8%. The berberine content of fruit of Berberis aristata and the herbal formulation were 0.033% and 0.0089% (w/w), respectively. This HPLC method for quantification of berberine can be used for quality control and standardization of several crude drugs and different herbal formulations in which berberine is present as a phyto constituent.

  • [1]. Anonymous, The Wealth of India, A Dictionary of Indian Raw Materials and Industrial Products, Publications & Information Directorate, CSIR, New Delhi, 1988, pp, 114–118.

  • [2]. Anonymous, The Unani Pharmacopoeia of India, Ministry of Health and Family Welfare, Department of AYUSH, Govt. of India, New Delhi, 2007, pp, 32–33.

    • Search Google Scholar
    • Export Citation

  • [3]. A.K. Gupta M. Sharma N. Tandon 2004 Reviews on Indian Medicinal Plants Indian Council of Medical Research New Delhi 165–185.

  • [4]. Anonymous, The Ayurvedic Pharmacopoeia of India, Ministry of Health and Family Welfare, Department of AYUSH, Govt. of India, New Delhi, 1999, pp, 34–35.

  • [5]. Anonymous, National Formulary of Unani Medicine, Ministry of Health and Family Welfare, Department of AYUSH, Govt. of India, New Delhi, 2006, pp, 30–31.

  • [6]. S. Letasiova S. Jantova M. Muckova M. Theiszova 2005 Biomed. Pap. Med. Fac. Univ. Palacky Olomouc Czech Repub. 149 461.

  • [7]. H. Rajaian J. Jalaee A. Aghajani 2006 Int. J. Poultry Sci. 5 395.

  • [8]. M. Pitea P. Petcu T. Goina N. Preda 1972 Planta Med. 21 177.

  • [9]. W. Li J.F. Fitzloff 2002 J. Pharm. Pharmacol. 54 435.

  • [10]. G. Srinivasan K. Unnikrishnan A. Rema Shree I. Balachandran 2008 Indian J. Pharm. Sci. 70 96.

  • [11]. C.M. Chen H.C. Chang 1995 J. Chromatogr. B 665 117.

  • [12]. A. Kong X. Li Z. Ni Y. Wang 2004 Se Pu. 22 89.

  • [13]. Y.R. Li Y. Jiang F. Hao X.Y. Ding 2007 Zhongguo Zhong Yao Za Zhi 32 2597.

  • [14]. C. Ma M. Fan Y. Tang Z. Li Z. Sun G. Ye C. Huang 2008 Biomed. Chromatogr. 22 835.

  • [15]. P.K. Gupta M. Hubbard B. Gurley H.P. Hendrickson 2009 J. Pharm. Biomed. Anal. 49 1021.

  • [16]. K.K. Rout S. Pradhan S.K. Mishra 2008 J. AOAC Int. 91 1149.

  • [17]. International Conference on Harmonization (ICH) of Technical Requirements for the Registration of Pharmaceuticals for Human Use, Validation of Analytical Procedures: Methodology, Geneva, 1996.

    • Search Google Scholar
    • Export Citation

  • [18]. M.J. Ansari S. Ahmad K. Kohli J. Ali R.K. Khar 2005 J. Pharm. Biomed. Anal. 39 132.

  • [19]. S. Ahmad M. Rizwan R. Parveen M. Mujeeb M. Aquil 2008 Chromatographia 67 441.

  • [20]. M.A. Bhat S. Ahmad A. Mujib M. Zafar 2008 Chem. Pharm. Bull. 56 17.

  • [21]. P. Alam M. Ali R. Singh Madhurima S. Ahmad F. Shakeel 2009 J. Chromatogr. Sci. 47 910.

  • [22]. S. Baboota S. Faiyaz A. Ahuja J. Ali S. Shafiq S. Ahmad 2007 Acta Chromatogr. 18 116.

  • [23]. N. Ahmad J. Ali S. Baboota S. Ahmad 2008 Acta Chromatogr. 20 277.

  • [24]. R. Parveen S. Ahmad S. Baboota J. Ali A. Ahuja 2010 Biomed. Chromatogr. 24 639.
Cover Acta Chromatographica
Acta Chromatographica
Online ISSN:
2083-5736
Keywords:
berberine; HPLC; Berberis aristata ; Unani formulation

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Aug 2020 7 0 0
Sep 2020 18 0 0
Oct 2020 13 0 0
Nov 2020 6 1 0
Dec 2020 20 0 0
Jan 2021 2 0 0
Feb 2021 0 0 0