View More View Less
  • 1 Faculty of Pharmacy, Department of Pharmaceutics, Hamdard University (Jamia Hamdard), Hamdard Nagar, New Delhi, 110 062, India
  • 2 Faculty of Pharmacy, Department of Pharmacognosy and Phytochemistry, Hamdard University (Jamia Hamdard), Hamdard Nagar, New Delhi, 110 062, India
Restricted access

Summary

In this chapter, an isocratic reverse phase HPLC determination of mimosine was developed and validated in an anti-psoriatic topically applied formulation “Lajjalu”. The chromatography was performed on a C18 column with water-orthophosphoric acid (98.8:0.2, υ/υ) as a mobile phase with a pH of 3.0 at a flow rate of 1.0 mL min−1. Detection was performed at 284 nm, and a sharp peak was obtained for mimosine at a retention time of 2.62 ± 0.01 min. Linear regression analysis data for the calibration plot showed a good linear relationship between response curve and concentration in the range of 0.050–5000 ng mL−1 and the regression coefficient was 0.9998 with the linear regression equation y = 4766.8x−17726. The detection (LOD) and quantification (LOQ) limits were 10.3 and 35.6 ng mL−1, respectively. The wide linearity range, sensitivity, accuracy, short retention time, and simple mobile phase imply the method is suitable for routine quantification of mimosine with high precision and accuracy.

  • [1]. K.B. Gordon R.R. Blum K.A. Papp R. Matheson C. Bolduc T. Hamilton M. Heffernan M.A. Menter M.M. Okun J. Zhong M. Kaul A. Camez 2007 Psoriasis Forum 13 411.

    • Search Google Scholar
    • Export Citation
  • [2]. A.J. Hueber I.B. McInnes 2007 Immunol. Lett. 114 5965.

  • [3]. Nimbatiktam for the Treatment of Psoriasis, http://www.nrdcindia.com/pages/nimbatiktam.htm [last accessed on 18 January 2010].

  • [4]. J.A. Duke and M.J. Bogenschutz-Godwin, Handbook of Medicinal Herbs, 2nd edn, CRC Press, 2002, pp. 663664.

  • [5]. R. Puchala S.G. Pierzynowski T. Sahlu S.P. Hart 1996 Br. J. Nutr. 15 6979.

  • [6]. M. Soedarjo T.K. Hemscheidt D. Borthakur 1994 Appl. Environ. Microbiol. 60 42684272.

  • [7]. S. Vaithiyanathan Q. Sheikh R. Kumar 2005 Asian Austral. J. Anim. Sci. 18 332339.

  • [8]. P.R. Abel and G. Ruth, World Patent WO2007129133 (2007).

  • [9]. G.Z. Moises , M.L. Ruben, and P.D. Antonio, European Patent 20070704740 (2008).

  • [10]. L.S. Nair S.N. Menon S. Shailajan M.M. Baing R.T. Sane 2007 J. Planar Chromatogr. 20 4951.

  • [11]. K. Lalitha S.R. Kulothungan 2004 Talanta 63 635640.

  • [12]. K. Lalitha S.R. Kulothungan 2006 Amino Acids 31 279287.

  • [13]. R.G. Megarrity 2006 J. Sci. Food Agric. 29 182186.

  • [14]. R. Puchala J.J. Davis T. Sahlu 1996 J. Chromatogr., B 685 375378.

  • [15]. CH, Q2 (R1), Validation of Analytical Procedures: Text and Methodology. International Conference on Harmonization, Geneva, http://www.ich.org/LOB/media/MEDIA417.pdf (2005).

    • Search Google Scholar
    • Export Citation
  • [16]. S. Baboota S. Faiyaz A. Ahuja J. Ali S. Ahmad 2007 Acta. Chromatogr. 18 116129.

  • [17]. M.J. Ansari S. Ahmad K. Kohli J. Ali R.K. Khar 2005 J. Pharm. Biomed. Anal. 39 132136.

  • [18]. S. Ahmad R. Ahmad R. Parveen M. Aquil 2008 Chromatographia 67 4147.

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jun 2020 0 3 1
Jul 2020 2 0 0
Aug 2020 6 0 0
Sep 2020 1 0 0
Oct 2020 2 0 0
Nov 2020 1 1 0
Dec 2020 0 0 0