View More View Less
  • 1 State Key Lab of Advanced Technology for Platinum Metals, Kunming Institute of Precious Metals, Kunming, 650106, China
  • | 2 School of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming, 650092, China
Restricted access

Summary

A simple and rapid HPLC method using a photodiode array (PDA) detector for the analysis of 3-hydroxycarboplatin and its related complex has been established for the first time. Separation of 3-hydroxycarboplatin and 3-hydroxy-1,1-cyclobutanedicarboxylic acid (3-HO-cbdca) was carried out on a Phenomenex ODS3 column using an aqueous solution containing 50 mM ammonium acetate and 5 mM sodium 1-octanesulfonate as the mobile phase. The flow rate was 0.8 mL min−1, the column temperature was 40°C, and the detection wavelength was 230 nm for 3-hydroxycarboplatin and 220 nm for 3-HO-cbdca. Different analytical performance parameters such as precision, accuracy, linearity, stability of the solution, specificity, limit of detection (LOD), limit of quantification (LOQ), and system suitability were determined using the Empower 2 software. The calibration curve of standard 3-hydroxycarboplatin showed good linearity (r = 0.9995) within the range 0.5–1.4 mg mL−1. The method was accurate and precise, with an average accuracy of 100.4% (RSD = 1.53%, n = 9), and the results of the system suitability test showed symmetrical peaks, good resolution (Rs), and repeatability. It can be applied to the quality control of 3-hydroxycarboplatin.

  • [1]. E. Wang C.M. Giandomenico 1999 Chem. Rev. 99 2451.

  • [2]. Y.P. Ho S.C.F. Au-Yeung K.K.W. To 2003 Med. Res. Rev. 23 633.

  • [3]. G. Momekov A. Bakalova M. Karaivanova 2005 Curr. Med. Chem. 12 2177.

  • [4]. S.V. Zutphen J. Reedijk 2005 Coord. Chem. Rev. 249 2845.

  • [5]. A.S. Abu-Surrah M. Kettunen 2006 Curr. Med. Chem. 13 1337.

  • [6]. M.A. Jakupec M. Galanski B.K. Keppler 2003 Rev. Physiol. Biochem. Pharmacol. 146 1.

  • [7]. M. Galanski 2006 Recent Patents on Anticancer Drug Discovery 1 285.

  • [8]. G. Bernhardt H. Brunner N. Gruber 2004 Inorg. Chim. Acta 357 4452.

  • [9]. A.L. Flores R. Jurado P.G. Lopez 2005 J. Pharmacol. Toxicol. Methods 52 370.

  • [10]. M.E. Bosch A.J.R. Sanchez F.S. Rojas C.B. Ojeda 2008 J. Pharm. Biomed. Anal. 47 453.

  • [11]. A. Mittal D. Chitkara N. Kumar 2007 J. Chromatogr. B 855 212.

  • [12]. D.N. Bell J.J. Liu M.D. Tingle M.J. McKeage 2006 J. Chromatogr. B 837 31.

  • [13]. Y. Vander Heyden D. Mangelings Van Brempt H. Spapen 2004 Acta Chromatogr. 14 155.

  • [14]. Z. Mrkvickova P. Kovarikova J. Klimes 2007 J. Pharm. Biomed. Anal. 43 1347.

  • [15]. Chinese Pharmacopia , 2005, p, 108.

  • [16]. S. Hann Zs. Stefanka K. Lenz G. Stingeder 2005 Anal. Bioanal. Chem. 381 408.

  • [17]. W. Zapała K. Kaczmarski 2010 Acta Chromatogr. 22 18.

  • [18]. USP, 2007, 30th edn, p, 1630.

All Time Past Year Past 30 Days
Abstract Views 17 17 0
Full Text Views 3 2 0
PDF Downloads 1 0 0