View More View Less
  • 1 Department of Pharmacognosy, ISF College of Pharmacy, Moga, 142001, India
Restricted access

Summary

A simple, selective, precise, and reproducible high-performance thin-layer chromatography (HPTLC) method for the analysis of vicine in herbal extracts and formulations containing Momordica charantia was developed. The study was performed on aluminum-based pre-coated TLC plates (silica gel 60F254). The chromatograms of samples were developed in twin trough glass chamber pre-saturated with mobile phase comprised of ethyl acetate-methanol-water-formic acid (7.5:3:1:0.1, v/v/v/v) at room temperature (25 ± 2°C). The densitometric analysis was carried out in absorbance mode at 278 nm. The optimized mobile phase showed a compact spot of vicine (R F = 0.31 ± 0.02) from the other constituents present in the samples. The linear regression analysis data for the calibration plots showed good linearity (r 2 = 0.9990) with respect to peak area in the range of 200–1400 ng spot−1. The method was validated as per International Conference on Harmonization (ICH) guidelines. The limits of detection and quantification (50 and 150 ng spot−1, respectively) were also established. The proposed method has shown the excellent recovery (98.01–99.33%), which supports the suitability of the method for the analysis. Statistical analysis of the obtained data showed the selectivity of the proposed method for vicine estimation in the herbal samples.

  • [1]. J. K. Grover S. P. Yadav 2004 J. Ethnopharmacol. 93 123.

  • [2]. J. Virdi S. Sivakami S. Shahani A.C. Suthar M.M. Banavalikar M.K. Biyani 2003 J. Ethnopharmacol. 88 107.

  • [3]. M. Tan J. Ye N. Turner C. Hohnen-Behrens C. Ke C. Tang T. Chen H. Weiss E. Gesing A. Rowland D. E. James Y. Ye 2008 Chem. Biol. 15 263.

    • Search Google Scholar
    • Export Citation
  • [4]. S. Omar Z. Ansari M. Nehal 2007 Fitoterapia 78 46.

  • [5]. J. E. Cunnick K. Sakamoto S. K. Chapes G. W. Fortner D. J. Takemoto 1990 Cell Immunol. 126 278.

  • [6]. J. H. Kim E. M. Ju D. K. Lee H. J. Hwang 2002 Anticancer Res. 22 1885.

  • [7]. Y. Hwang J. W. Lee E. Hahm K. C. Jung J. H. Lee C. H. Park H. S. Rhee J. M. Ryu H. Kim C. Yang 2005 Biochem. Biophys. Res. Commun. 337 815.

    • Search Google Scholar
    • Export Citation
  • [8]. I. Ahmed M. S. Lakhani M. Gillett A. John H. Raza 2001 Diabetes Res. Clin. Pract. 51 155.

  • [9]. P. Chaturvedi 2005 Br. J. Biomed. Sci. 62 124.

  • [10]. P. Nerurkar Y. K. Lee E. H. Linden S. Lim L. Pearson J. Frank V. R. Nerurkar 2006 Br. J. Pharmacol. 148 1156.

  • [11]. N. M. Ansari L. Houlihan B. Hussain A. Pieroni 2005 Phytother. Res. 19 907.

  • [12]. I. Gurbuz C. Akyuz E. Yesilada B. Sener 2000 J. Ethnopharmacol. 7 77.

  • [13]. A. R. Biswas S. Ramaswamy J. S. Bapna 1991 J. Ethnopharmacol. 31 115.

  • [14]. J. Choi K. T. Lee H. Jung H. S. Park H. J. Park 2002 Arch. Pharm. Res. 25 336.

  • [15]. A. Sharanabasappa B. V. Saraswati B. Patil 2002 Pharm. Biol. 40 501.

  • [16]. V. P. Dixit P. Khanna S. K. Bhargava 1978 Planta Med. 34 280.

  • [17]. A. Raman C. Lau 1996 Phytomedicine 2 349.

  • [18]. S. I. Kim E. Hoehn N. A. M. Eskin F. Ismail 1982 J. Agric. Food Chem. 30 144.

  • [19]. H. S. Olsen J. H. Andersen 1978 J. Sci. Food Agric. 29 323.

  • [20]. H. Zhang Y. Wang X. Zhang M. Liu Z. Hu 2003 Anal. Lett. 36 1597.

  • [21]. E. A. Abourashed J. S. Mossa 2004 J. Pharm. Biomed. Anal. 36 617.

  • [22]. H. Agarawal N. Kaul A. R. Paradkar K. R. Mahadik 2004 J. Pharm. Biomed. Anal. 36 33.

  • [23]. V.B. Patravale S. D'souza Y. Narkar 2001 J. Pharm. Biomed. Anal. 25 685.

  • [24]. International Conference on Harmonization, Q2A, Geneva, 2005.

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Aug 2020 1 0 0
Sep 2020 5 0 0
Oct 2020 17 0 0
Nov 2020 3 2 0
Dec 2020 0 0 0
Jan 2021 1 0 0
Feb 2021 0 0 0