A reliable, sensitive and rapid method for determination of nicotine and three minor alkaloids (cotinine, anabasine and nornicotine) in tobacco by ultra-high performance liquid chromatography in hydrophilic interaction chromatography mode coupled with tandem mass spectrometry (HILIC-MS/MS) has been established. HILIC separation was performed on a BEH HILIC column using isocratic elution at 0.5 mL/min with acetonitrile:water (85:15, v/v) mobile phase containing 5 mmol/L ammonium acetate (pH 5.00). Separated analytes were determined by electrospray ionization MS/MS in the positive ion mode using multiple reaction monitoring. Alkaloids from tobacco were extracted in an ultrasonic bath for 10 min with acetonitrile:water mixture (8:2, v/v) containing 5 mmol/L ammonium acetate (pH 5.00). Limits of quantification were 10 μg/g for cotinine, 20 μg/g for anabasine and nornicotine, and 30 μg/g for nicotine. Mean recoveries from tobacco ranged between 94.8% and 104.1% for different analytes with relative standard deviations within 5%. The performance of the proposed method was tested for the extraction and determination of the four alkaloids in cigarette tobacco fillers, and satisfactory results were achieved.
A. Rodgman and T. A. Perfetti, The chemical components of tobacco and tobacco smoke, Taylor & Francis, Boca Raton, FL, 2008, pp. 780–789
Perfetti T. A., '', in The chemical components of tobacco and tobacco smoke, (2008) -.
Perfetti T. A.The chemical components of tobacco and tobacco smoke2008)| false
D. Yildiz, Toxicon, 43, 619 (2004)
Yildiz D., '' (2004) 43Toxicon: 619-.
Yildiz D.Toxicon200443619)| false
J. M. Badr, F. H. Bamane, and N. S. El-Shaer, J. Liq. Chrom. Relat. Tech., 35, 1213 (2012)