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  • 1 Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh, 11451, Saudi Arabia
  • | 2 Medicinal, Aromatic and Poisonous Plants Research Center (MAPPRC), College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh, 11451, Saudi Arabia
  • | 3 Department of Pathology, King Khalid University Hospital, King Saud University, P.O. Box 2925, Riyadh, 11461, Saudi Arabia
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Abstract

Hibiscus sabdariffa L. (Roselle) is a vegetable known to possess various therapeutic properties. We evaluated the anti-ulcerogenic property of ethanolic extract of dried calyces (EEHS) in different ulcer models in Wistar albino rats. The extract at 250 and 500 mg/kg body weight, orally has a significant effect in cold restraint stress, pylorus ligation, necrotizing agents (80% ethanol, 0.2 M NaOH and 25% NaCl) and indomethacin-induced gastric ulcer models. The extract showed an ability to significantly protect against gastric mucosal injury in all models used. Furthermore, EEHS has significantly decreased the basal gastric acid secretion, as well as significantly increased gastric wall mucus secretion (GWM) and non-protein sulfhydryl (NP-SH) concentrations in gastric tissue. Whereas, the extract significantly reduced the ethanol-induced elevated levels of malondialdehyde (MDA) in the rat stomach. These pharmacological and biochemical findings were further supported by the histological assessment of the stomach. The phytochemical constituents present in the H. sabdariffa calyces may contribute to its anti-ulcer activity through one or more mechanism(s), including the antisecretory and antioxidant nature of the extract.

  • [1]. J. E. Roberts V. E. Tyler 1999 Tyler's Herbs of Choice. The Therapeutic Use of Phytomedicinals The Haworth Herbal Press New York 11.

    • Search Google Scholar
    • Export Citation
  • [2]. M. A. Andreo K. V. R. Ballsteros C. A. Hiruma-Lima et al.2006 Effects of Mouriri pusa extracts on experimentally induced gastric lesions in rodents: role of endogenous sulfhydryls and nitric oxide in gastroprotection J. Ethanopharmacol. 107 431441.

    • Search Google Scholar
    • Export Citation
  • [3]. D. A. Lewis P. J. Hanson 1991 Antiulcer drugs of plant origin Prog. Med. Chem. 28 201231.

  • [4]. R. N. Chopra S. L. Neyar I. C. Chopra 1956 Glossary of Indian Medicinal Plants Council of Scientific & Industrial Research New Delhi 133.

    • Search Google Scholar
    • Export Citation
  • [5]. V. Hirumpanich A. Utaipat N. P. Morales et al.2005 Antioxidant effects of aqueous extracts from dried calyx of Hibiscus sabdariffa Linn. (Roselle) in vitro using rat low-density lipoprotein (LDL) Biol. Pharm. Bull. 28 481484.

    • Search Google Scholar
    • Export Citation
  • [6]. H. Mozaffari-Khosravi B. A. Jalali-Khanabadi M. Afkhami-Ardekani et al.2009 The effects of sour tea (Hibiscus sabdariffa) on hypertension in patients with type II diabetes J. Hum. Hypertens. 23 4854.

    • Search Google Scholar
    • Export Citation
  • [7]. H. H. Lin H. P. Huang C. C. Huang et al.2005 Hibiscus polyphenol-rich extract induces apoptosis in human gastric carcinoma cells via p53 phosphorylation and p38 MAPK/FasL cascade pathway Mol. Carcinog. 43 8699.

    • Search Google Scholar
    • Export Citation
  • [8]. A. Adetutu O. A. Odunola O. A. Owoade et al.2004 Anticlastogenic effects of Hibiscus sabdariffa fruits against sodium arsenite-induced micronuclei formation in erythrocytes in mouse bone marrow Phytother. Res. 18 862864.

    • Search Google Scholar
    • Export Citation
  • [9]. M. B. Ali W. M. Salih A. H. Mohamed et al.1991 Investigation of the antispasmodic potential of Hibiscus sabdariffa calyces J. Ethnopharmacol. 31 249257.

    • Search Google Scholar
    • Export Citation
  • [10]. B. H. Ali H. M. Mousa S. El-Mougy 2003 The effect of a water extract and anthocyanins of Hibiscus sabdariffa L. on paracetamol-induced hepatoxicity in rats Phytother. Res. 17 5659.

    • Search Google Scholar
    • Export Citation
  • [11]. A. M. Salah J. Gathumbi W. Vierling 2002 Inhibition of intestinal motility by methanol extracts of Hibiscus sabdariffa L. (Malvaceae) in rats Phytother. Res. 16 283285.

    • Search Google Scholar
    • Export Citation
  • [12]. A. Herrera-Arellano S. Flores-Romero M. A. Chavez-Soto et al.2004 Effectiveness and tolerability of a standardized extract from Hibiscus sabdariffa in patients with mild to moderate hypertension: a controlled and randomized clinical trial Phytomedicine 11 375382.

    • Search Google Scholar
    • Export Citation
  • [13]. B. O. Mojiminiyi B. J. Adegunloye Y. A. Egbeniyi et al.2000 An investigation of the diuretic effect of an aqueous extract of the petals of Hibiscus sabdariffa J. Med. Sci. 2 7780.

    • Search Google Scholar
    • Export Citation
  • [14]. C. C. Chen J. D. Hsu S. F. Wang et al.2003 Hibiscus sabdariffa extract inhibits the development of atherosclerosis in cholesterol-fed rabbits J. Agric. Food Chem. 51 54725477.

    • Search Google Scholar
    • Export Citation
  • [15]. V. Hirunpanich A. Utaipat N. P. Morales et al.2006 Hypocholes-terolemic and antioxidant effects of aqueous extracts from the dried calyx of Hibiscus sabdariffa L. in hypercholesterolemic rats J. Ethnopharmacol. 103 252260.

    • Search Google Scholar
    • Export Citation
  • [16]. N. Gaet 1999 Hibiscus sabdariffa L A. Iva Medicinal Plants of the World Human Press New York 165170.

  • [17]. E. C. Senay R. L. Levine 1967 Synergism between cold and restraint for rapid production of stress ulcer in rats Proc. Soc. Exp. Biol. Med. 124 12211231.

    • Search Google Scholar
    • Export Citation
  • [18]. P. J. S. Chiu C. Gerhart A. D. Brown et al.1984 Effects of a gastric antisecretory cytoprotectant 2-methyl-8-(phenylmethoxy)imidazo (1,2-a)-pyridine-3-acetonitrile (Sch 28080) on cysteamine, reserpine and stress ulcers in rats Arzneim. Forsch. 34 783.

    • Search Google Scholar
    • Export Citation
  • [19]. H. Shay S. A. Komarov S. S. Fels et al.1945 A simple method for the uniform production of gastric ulceration in the rat Gastroenterology 5 4361.

    • Search Google Scholar
    • Export Citation
  • [20]. A. Robert J. E. Nezamis C. Lancaster et al.1983 Mild irritants prevent gastric necrosis through adaptive cytoprotection mediated by prostaglandins Am. J. Physiol. 245 G113.

    • Search Google Scholar
    • Export Citation
  • [21]. S. J. Crone S. M. Morrissey R. J. Woods 1974 A method for the quantitative estimation of gastric barrier mucus J. Physiol. 242 116117.

    • Search Google Scholar
    • Export Citation
  • [22]. K. P. Bhargawa M. B. Gupta K. K. Tangri 1973 Mechanism of ulcerogenic activity of indomethacin and oxyphenbutazone Eur. J. Pharmacol. 22 191195.

    • Search Google Scholar
    • Export Citation
  • [23]. J. Sedlak R. H. Lindsay 1968 Estimation of total protein bound and nonprotein sulfhydryl group in tissue with Ellman's reagents Anal. Biochem. 25 192205.

    • Search Google Scholar
    • Export Citation
  • [24]. H. G. Utley F. Bernheim P. Hochstein 1967 Effect of sulfhydryl reagents on peroxidation in microsomes Arch. Biochem. Biophys. 118 2932.

    • Search Google Scholar
    • Export Citation
  • [25]. C. F. Culling 1974 Handbook of Histopathological and Histochemical Techniques 3rd edn. Butterworth and Co. London 37.

  • [26]. B. Djahanguiri H. L. Taubin L. Landsburg 1973 Increased sympathetic activity in the pathogenesis of restraint ulcer in rats J. Pharmacol. Exp. Ther. 184 163168.

    • Search Google Scholar
    • Export Citation
  • [27]. C. H. Cho C. W. Ogle S. Dai 1976 Acute gastric ulcer formation response to electrical vagal stimulation in rats Eur. J. Pharmacol. 35 215219.

    • Search Google Scholar
    • Export Citation
  • [28]. C. H. Cho C. W. Ogle 1979 Cholinergic-mediated gastric mast cell degranulation with subsequent histamine H1 and H2-receptor activation in stress ulceration in rats Eur. J. Pharmacol. 55 2333.

    • Search Google Scholar
    • Export Citation
  • [29]. T. Hase B. J. Moss 1973 Microvascular changes of gastric mucosa in the development of stress ulcer in rats Gastroenterology 65 224234.

    • Search Google Scholar
    • Export Citation
  • [30]. R. K. Goel S. K. Bhattacharya 1991 Gastroduodenal mucosal defense and mucosal protective agents Indian J. Exp. Biol. 29 701714.

    • Search Google Scholar
    • Export Citation
  • [31]. G. Schmeda-Hirschmann J. Rodriguez L. Astudillo 2002 Gastroprotective activity of the diterpene solidagenone and its derivatives on experimentally induced gastric lesions in mice J. Ethnopharmacol. 81 111115.

    • Search Google Scholar
    • Export Citation
  • [32]. J. S. Trier S. Szabo C. H. Allan 1987 Ethanol-induced damage to mucosal capillaries of rat stomach. Ultrastructural features and effects of prostaglandin E2 and cysteamine Gastroenterology 92 1322.

    • Search Google Scholar
    • Export Citation
  • [33]. T. Matsumoto R. Moriguchi H. Yamada 1992 Role of polymorphonuclear leucocytes and oxygen-derived free radicals in the formation of gastric lesions induced by HCl/ethanol, and a possible mechanism of protection by antiulcer polysaccharide J. Pharm. Pharmacol. 45 535539.

    • Search Google Scholar
    • Export Citation
  • [34]. C. Farina M. Pinza G. Pifferi 1998 Synthesis and anti-ulcer activity of new derivatives of glycyrrhetic, oleanolic and ursolic acids Il Farmaco 53 2232.

    • Search Google Scholar
    • Export Citation
  • [35]. M. K. Jainu V. Mohan C. S. S. Devi 2006 Gastroprotective effect of Cissus quadrangularis extract in rats with experimentally induced ulcer Indian J. Med. Res. 123 799806.

    • Search Google Scholar
    • Export Citation
  • [36]. F. Younau J. Person A. Allen et al.1982 Changes in the structure of the mucus gel in the mucosal surface of the stomach in association with peptic ulcer disease Gastroenterology 82 827831.

    • Search Google Scholar
    • Export Citation
  • [37]. A. K. Sanyal P. K. Mitra R. K. Goel 1983 A modified method to estimate dissolved mucosubstance in gastric juice Indian J. Exp. Biol. 21 7880.

    • Search Google Scholar
    • Export Citation
  • [38]. J. Wallace 2001 Mechanisms of protection and healing: current knowledge and future research Am. J. Med. 110 19S22S.

  • [39]. K. D. Rainsford 1987 The effects of 5-lipoxygenase inhibitors and leukotriene antagonists on the development of gastric lesions induced by non-steroidal anti-inflammatory drugs Agents Actions 21 316319.

    • Search Google Scholar
    • Export Citation
  • [40]. B. J. Whittle 1981 Temporal relationship between cycloxygenase inhibition, as measured by prostacyclin biosynthesis, and the gastrointestinal damage induced by indomethacin in rat Gastroenterology 80 9498.

    • Search Google Scholar
    • Export Citation
  • [41]. S. Szabo J. S. Trier P. W. Frankel 1981 Sulfhydral compounds may mediate gastric cytoprotection Science 214 200202.

  • [42]. S. Alqasoumi M. Al-Sohaibani T. Al-Howiriny et al.2009 Rocket 'Eruca sativa’: a salad herb with potential gastric anti-ulcer activity World J. Gastroenterol. 15 19581965.

    • Search Google Scholar
    • Export Citation
  • [43]. S. C. Boyd H. A. Sasame M. R. Boyd 1979 High concentrations of glutathione in glandular stomachs. Possible implication for carcinogenesis Science 205 10101012.

    • Search Google Scholar
    • Export Citation
  • [44]. H. J. Stein R. A. Hinder M. M. J. Oosthuizeu 1990 Gastric mucosal injury caused hemorrhagic shock and reperfusion: protective role of the antioxidant glutathione Surgery 108 467474.

    • Search Google Scholar
    • Export Citation
  • [45]. S. Kwicien T. Brzozowski S. J. Konturek 2002 Effects of reactive oxygen species action on gastric mucosa in various models of mucosal injury J. Physiol. Pharmacol. 53 3950.

    • Search Google Scholar
    • Export Citation

Clinical and Experimental Medical Journal
Language English
Size  
Year of
Foundation
2007
Publication
Programme
ceased
Volumes
per Year
 
Issues
per Year
 
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 2060-6249 (Print)
ISSN 2060-968X (Online)

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