Abstract
24p3 (also called SIP 24, uterocalin, neutrophil-gelatinase-associated lipocalin (NGAL), lipocalin 2, and siderocalin) is an acute phase protein induced under many inflammatory situations which regulates iron delivery and may play an important role in innate immunity. In this study, we examined whether 24p3 is induced in the course of Concanavalin- A (Con-A)-induced autoimmune hepatitis. Upon intravenous injection of Con-A into mice, 24p3 mRNA expression in the liver as detected by real-time quantitative RT-PCR was significantly increased by 20–28-fold. Lung and lymphoid tissues such as thymus, lymph nodes, and spleen were also found to maintain high levels of 24p3 mRNA. No induction of 24p3 mRNA was found in other tissues, including muscle, heart, brain, and kidney. Consistent with the PCR results, 24p3 protein levels detected by Western blot were increased in a time-dependent manner after Con-A injection. Our results showed that 24p3 expression is induced in Con-A-mediated acute liver failure at both transcriptional and translational levels. 24p3 might play a role in the feedback control to limit inflammation in the liver.
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[1] Tiegs, G., Hentschel, J., Wendel, A. 1992 A T cell-dependent experimental liver injury in mice inducible by concanavalin A J. Clin. Invest. 90 196–203.
-
[2] Mizuhara, H., O’Neill, E., Seki, N. 1994 T cell activation-associated hepatic injury: mediation by tumor necrosis factors and protection by interleukin 6 J. Exp. Med. 179 1529–1537.
-
[3] Gantner, F., Leist, M., Lohse, A. W. 1995 Concanavalin A-induced T-cell-mediated hepatic injury in mice: the role of tumor necrosis factor Hepatology 21 190–198.
-
[4] Pratt, D. S., Kaplan, M. M. 2000 Evaluation of abnormal liver-enzyme results in asymptomatic patients N. Engl. J. Med. 342 1266–1271.
-
[5] Takeda, K., Hayakawa, Y., Kaer Van, L. 2000 Critical contribution of liver natural killer T cells to a murine model of hepatitis Proc. Natl. Acad. Sci. USA 97 5498–5503.
-
[6] Sass, G., Heinlein, S., Agli, A. 2002 Cytokine expression in three mouse models of experimental hepatitis Cytokine 19 115–120.
-
[7] Mizuhara, H., Uno, M., Seki, N. 1996 Critical involvement of interferon gamma in the pathogenesis of T-cell activation-associated hepatitis and regulatory mechanisms of interleukin-6 for the manifestations of hepatitis Hepatology 23 1608–1615.
-
[8] Louis, H., Moine Le, O., Goldman, M., Deviere, J. 2003 Modulation of liver injury by interleukin-10 Acta Gastroenterol. Belg. 66 7–14.
-
[9] Streetz, K. L., Wustefeld, T., Klein, C. 2001 Mediators of inflammation and acute phase response in the liver Cell. Mol. Biol. 47 661–673.
-
[10] Uyttenhove, C., Coulie, P. G., Snick Van, J. 1988 T cell growth and differentiation induced by interleukin-HP1/IL-6, the murine hybridoma/plasmacytoma growth factor J. Exp. Med. 167 1417–1427.
-
[11] Radaeva, S., Sun, R., Pan, H. N. 2004 Interleukin 22 (IL-22) plays a protective role in T cell-mediated murine hepatitis: IL-22 is a survival factor for hepatocytes via STAT3 activation Hepatology 39 1332–1342.
-
[12] Nilsen-Hamilton, M., Hamilton, R. T., Adams, G. A. 1982 Rapid selective stimulation by growth factors of the incorporation by BALB/C 3T3 cells of [35S]methionine into a glycoprotein and five superinducible proteins Biochem. Biophys. Res. Commun. 108 158–166.
-
[13] Hraba-Renevey, S., Turler, H., Kress, M. 1989 SV40-induced expression of mouse gene 24p3 involves a posttranscriptional mechanism Oncogene 4 601–608.
-
[14] Hamilton, R. T., Nilsen-Hamilton, M., Adams, G. 1985 Superinduction by cycloheximide of mitogen-induced secreted proteins produced by Balb/c 3T3 cells J. Cell. Physiol. 123 201–208.
-
[15] Davis, T. R., Tabatabai, L., Bruns, K. 1991 Basic fibroblast growth factor induces 3T3 fibroblasts to synthesize and secrete a cyclophilin-like protein and beta 2-microglobulin Biochim. Biophys. Acta 1095 145–152.
-
[16] Liu, Q., Nilsen-Hamilton, M. 1995 Identification of a new acute phase protein J. Biol. Chem. 270 22565–22570.
-
[17] Liu, Q., Ryon, J., Nilsen-Hamilton, M. 1997 Uterocalin: a mouse acute phase protein expressed in the uterus around birth Mol. Reprod. Dev. 46 507–514.
-
[18] Kjeldsen, L., Johnsen, A. H., Sengelov, H., Borregaard, N. 1993 Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase J. Biol. Chem. 268 10425–10432.
-
[19] Flower, D. R., North, A. C., Attwood, T. K. 1991 Mouse oncogene protein 24p3 is a member of the lipocalin protein family Biochem. Biophys. Res. Commun. 180 69–74.
-
[20] Flo, T. H., Smith, K. D., Sato, S. 2004 Lipocalin 2 mediates an innate immune response to bacterial infection by sequestrating iron Nature 432 917–921.
-
[21] Meheus, L. A., Fransen, L. M., Raymackers, J. G. 1993 Identification by microsequencing of lipopolysaccharide-induced proteins secreted by mouse macrophages J. Immunol. 151 1535–1547.
-
[22] Devireddy, L. R., Teodoro, J. G., Richard, F. A., Green, M. R. 2001 Induction of apoptosis by a secreted lipocalin that is transcriptionally regulated by IL-3 deprivation Science 293 829–834.
-
[23] Devireddy, L. R., Gazin, C., Zhu, X., Green, M. R. 2005 A cell-surface receptor for lipocalin 24p3 selectively mediates apoptosis and iron uptake Cell 123 1293–1305.
-
[24] Berger, T., Togawa, A., Duncan, G. S. 2006 Lipocalin 2-deficient mice exhibit increased sensitivity to Escherichia coli infection but not to ischemia-reperfusion injury Proc. Natl. Acad. Sci. USA 103 1834–1839.
-
[25] Shen, F., Ruddy, M. J., Plamondon, P., Gaffen, S. L. 2005 Cytokines link osteoblasts and inflammation: microarray analysis of interleukin-17- and TNF-alpha-induced genes in bone cells J. Leukoc. Biol. 77 388–399.
-
[26] Shen, F., Hu, Z., Goswami, J., Gaffen, S. L. 2006 Identification of common transcriptional regulatory elements in interleukin-17 target genes J. Biol. Chem. 281 24138–24148.
-
[27] Karmen, A., Wroblewski, F., Ladue, J. S. 1955 Transaminase activity in human blood J. Clin. Invest. 34 126–131.
-
[28] Goetz, D. H., Holmes, M. A., Borregaard, N. 2002 The neutrophil lipocalin NGAL is a bacteriostatic agent that interferes with siderophore-mediated iron acquisition Mol. Cell 10 1033–1043.
-
[29] Yang, J., Goetz, D., Li, J. Y. 2002 An iron delivery pathway mediated by a lipocalin Mol. Cell 10 1045–1056.
-
[30] Mietzner, T. A., Morse, S. A. 1994 The role of iron-binding proteins in the survival of pathogenic bacteria Annu. Rev. Nutr. 14 471–493.
-
[31] Braun, V., Braun, M. 2002 Active transport of iron and siderophore antibiotics Curr. Opin. Microbiol. 5 194–201.
-
[32] Saiga, H., Nishimura, J., Kuwata, H. 2008 Lipocalin 2-dependent inhibition of mycobacterial growth in alveolar epithelium J. Immunol. 181 8521–8527.
-
[33] Chan, Y. R., Liu, J. S., Pociask, D. A. 2009 Lipocalin 2 is required for pulmonary host defense against Klebsiella infection J. Immunol. 182 4947–4956.
-
[34] Harrington, L. E., Hatton, R. D., Mangan, P. R. 2005 Interleukin 17-producing CD4+ effector T cells develop via a lineage distinct from the T helper type 1 and 2 lineages Nat. Immunol. 6 1123–1132.
-
[35] Park, H., Li, Z., Yang, X. O. 2005 A distinct lineage of CD4 T cells regulates tissue inflammation by producing interleukin 17 Nat. Immunol. 6 1133–1141.
-
[36] Nagata, T., McKinley, L., Peschon, J. J. 2008 Requirement of IL-17RA in Con A induced hepatitis and negative regulation of IL-17 production in mouse T cells J. Immunol. 181 7473–7479.
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