Clinical and Experimental Medical Journal
Volume/Issue:
Volume 5: Issue 2-3
Serum fibrosis markers (Procollagen-III-peptide, hyaluronic acid, transforming growth factor β-1), aspartate-aminotransferase to platelet ratio index (APRI), and transient elastography (FibroScan) in patients with chronic HCV infection
Authors:
Gabriella Pár First Department of Medicine, University of Pécs, Pécs, Hungary
First Department of Medicine, University of Pécs, Ifjúság u. 13, H-7624, Pécs, Hungary

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Áron Vincze First Department of Medicine, University of Pécs, Pécs, Hungary

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Timea Berki Department of Immunology and Biotechnology, University of Pécs, Pécs, Hungary

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Attila Miseta Department of Laboratory Medicine, University of Pécs, Pécs, Hungary

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László Kereskai Department of Pathology, University of Pécs, Pécs, Hungary

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László Pajor Department of Pathology, University of Pécs, Pécs, Hungary

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Angela Oszter Department of Pathology, University of Pécs, Pécs, Hungary

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Ferenc Jakab Department of Medical Microbiology and Immunology, University of Pécs, Pécs, Hungary

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Zoltán Szereday Baranya County Hospital, Pécs, Hungary

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Ágnes Nagy First Department of Medicine, University of Pécs, Pécs, Hungary

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Béla Hunyady First Department of Medicine, University of Pécs, Pécs, Hungary

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Alajos Pár First Department of Medicine, University of Pécs, Pécs, Hungary

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Pages:
101–118
Online Publication Date:
01 Jun 2011
Publication Date:
01 Jun 2011
Article Category:
Research Article
DOI:
https://doi.org/10.1556/CEMED.5.2011.2.5
Keywords:
hepatitis C virus; noninvasive fibrosis markers; hyaluronic acid; procollagen-III-peptide; transforming growth factor-β; APRI score; liver stiffness; transient elastography; antiviral treatment; ALT, alanine-aminotransferase; APRI, aspartate-aminotransferase to platelet ratio index; AST, aspartate-aminotransferase; ECM, extracellular matrix; ELISA, enzyme-linked immunosorbent assay; F, fibrosis; HA, hyaluronic acid; HAI, histological activity index; HCV, hepatitis C virus; HCV-RNA, hepatitis C virus ribonucleic acid; IFN, interferon; kPa, kilopascal; LB, liver biopsy; LS, liver stiffness; PEG-IFN, PEGilated interferon; PNA, persistently normal alanine-aminotransferase; P-III-P, procollagen-III-peptide; P/R, peginterferon plus ribavirin treatment; RBV, ribavirin; RIA, radioimmuno assay; SAFE, Sequential Algorithm for Fibrosis Evaluation; SVR, sustained virological response; TE, transient elastography; TGFβ-1, transforming growth factor beta-1
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Abstract

Background: Although liver biopsy (LB) is the gold standard for the morphological diagnosis of diseases of the liver, recent noninvasive tests may also help in the evaluation of liver fibrosis. We have studied blood fibrosis markers and liver stiffness (LS) measurement to assess fibrosis in different forms of chronic hepatitis C virus (HCV) infection. Patients and methods: Out of 119 HCV-infected patients, 75 had biopsy-proven chronic hepatitis C, 24 had HCV cirrhosis, 20 individuals were symptom-free HCV carriers with persistently normal alanine aminotransferase (PNA), and 30 healthy blood donors served as controls. Wai's aspartate-aminotransferase (AST) to platelet ratio index (APRI) was calculated from serum AST and blood platelet number. Serum HCV-RNA (hepatitis C virus ribonucleic acid), procollagen-III-peptide (P-III-P) and hyaluronic acid (HA), plasma transforming growth factor β-1 (TGFβ-1), Knodell's histological activity index (HAI), and METAVIR fibrosis score were determined, and for LS measurement transient elastography (TE) (FibroScan) was applied. Results: In chronic hepatitis C patients, all fibrosis markers were significantly elevated compared to normal controls. HA, APRI, and LS values were highest in HCV cirrhosis and nonresponders to PEG-IFN + ribavirin (P/R) therapy. High P-III-P values occurred only in advanced fibrosis. Serum HA correlated with the fibrosis stage. Plasma TGFβ-1 was significantly higher in patients with chronic hepatitis C than in symptom-free HCV carriers, and it correlated with HAI. After 3–6 months of P/R therapy, both HA and TGFβ-1 levels significantly decreased even in virological nonresponders. APRI was 0.21 ± 0.05 in normal controls, 0.20 ± 0.08 in symptom-free HCV carriers, 0.70 ± 0.40 in patients with chronic hepatitis C, and 3.21 ± 2.3 in HCV cirrhosis cases. LS values were 5.10 ± 1.19 kPa in controls, 5.38 ± 1.37 kPa in HCV carriers with PNA, 9.67 ± 4.11 kPa in chronic hepatitis C patients, 6.56 ± 2.61 kPa in patients with sustained virological response, 18.55 ± 11.65 kPa in nonresponders to P/R, and 37.40 ± 16.85 kPa in HCV cirrhosis cases. On the basis of a novel sequential algorithm that comprises APRI and LS for assessment of fibrosis, 47% of our HCV patients did not need biopsy for diagnosing significant (F ≥ 2) fibrosis. Conclusion: Both blood fibrosis markers and LS, particularly in combination, represent an advance in the noninvasive assessment of fibrosis in HCV infection. P/R treatment may inhibit fibrosis progression even independently of virological response.

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Cover Clinical and Experimental Medical Journal
Clinical and Experimental Medical Journal
Print ISSN:
2060-6249
Online ISSN:
2060-968X

Clinical and Experimental Medical Journal
Language English
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Foundation		 2007
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Publisher Akadémiai Kiadó
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ISSN 2060-6249 (Print)
ISSN 2060-968X (Online)

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