In this study, it was aimed to investigate the possible protective effect of an ethanolic extract of Basella alba L. on gentamycin (GM)-induced nephrotoxicity in Wistar albino rats. In the toxic group, rats were administered GM only (100 mg/kg, i.p.) for 8 days. In drug treated groups, rats were pretreated with B. alba (250 and 500 mg/kg per day orally) for 14 days and co-treated with GM for 8 days. After 24 h of the last dose, blood, urine, and tissue samples were collected from the animals. GM when administered induced a marked renal failure, characterized by a significant increase in serum and urine creatinine, urea, uric acid, gamma-glutamyl transferase (GGT), and protein levels. Besides, there were elevation of malondialdehyde (MDA) level and decrease in the concentration of total proteins (TPs) and free -SH in kidney tissue, which are indicators of oxidative stress of kidney. The extract also significantly reduced the GM-induced elevated serum and urine levels of sodium, potassium, calcium, protein, creatinine, urea, uric acid, and GGT. The tissue MDA level was also significantly diminished; the decreased free -SH and TP levels were significantly replenished by an ethanolic extract of B. alba treatment. The experimental results suggest that B. alba extract protected GM-induced nephrotoxicity, possibly by enhancing renal antioxidant system. The findings suggest the potential therapeutic use of B. alba as a novel nephroprotective agent.
[1]. C. Martínez-Salgado F. J. Lopez-Hernandez J. M. López-Novoa 2007 Glomerular nephrotoxicity of aminoglycosides Toxicol. Appl. Pharmacol. 223 86–98.
[2]. E. Arias R. N. Anderson H. C. Kung S. L. Murphy K. D. Kochanek 2003 Deaths: final data for 2001 Natl. Vital Stat Rep. 52 111–115.
[3]. P. Singh M. M. Srivastava L. D. Khemani 2009 Renoprotective effects of Andrographis paniculata (Burm. F.) Nees in rats Ups. J. Med. Sci. 114 136–139.
[4]. A. A. Al-Qarawi H. A. Rahman H. M. Mousa B. H. Ai S. A. El-Mougry 2008 Nephroprotective action of Phoenix dactylifera in gentamicin induced nephrotoxicity Pharmaceut. Biol. 46 227–230.
[5]. G. V. Harlalka C. R. Patil M. R. Patil 2007 Protective effect Kalanchoe pinnata pers. (Crassulaceae) on gentamicin induced nephrotoxicity in rats Indian J. Pharmacol. 39 201–205.
[6]. W. Dymock C. J. H. Warden D. Hooper 1890 Pharmacographia Indica: A History of the Principal Drugs of Vegetable Origin, Met with in British India Kegan Paul, Trench, Trübner & Co. Ltd. London 367.
[7]. L. M. Perry 1980 Medicinal Plants of East and Southeast Asia: Attributed Properties and Uses The MIT Press Cambridge, Massachusetts 54.
[8]. J. A. Duke E. S. Ayensu 1984 Medicinal Plants of China Reference Publications, Inc. Algonac, Michigan.
[9]. S. S. Hebba V. H. Harsha V. Shripathi G. R. Hegde 2004 Ethnomedicine of Dharwad district in Karnataka, India — Plants used in oral health care J. Ethnopharmacol. 94 261–266.
[10]. G. C. Yen H. Y. Chen H. H. Peng 2001 Evaluation of the chytotoxicity, mutagenecity and antimutagenecity of emerging edible plants Food Chem. Toxicol. 39 1045–1053.
[11]. G. D. Reddy R. Kartik Ch. V. Rao M. K. Unnikrishnan P. Pushpangadan 2008 Basella alba extract act as antitumor and antioxidant potential against N-nitrosodiethylamine induced hepatocellular carcinoma Int. J. Infect. Dis. 12 S3 S68.
[12]. N. R. Fransworth 1966 Biological and phytochemical screening of plants J. Pharm. Sci. 55 225–272.
[13]. Organization for Economic Cooperation and Development (OECD): OECD Guidelines for Testing of Chemicals. Acute Oral Toxicity, OECD (1993) No. 401.
[14]. T. Jeyanthi P. Subramanian 2009 Nephroprotective effect of Withania somnifera: a dose-dependent stu dy Ren. Fail. 31 814–821.
[15]. W. R. Moorhead H. C. Briggs 1974 2-Amino-2-methyl-1-propanol as the alkalizing agent in an improved continuous-flow cresolphthalein complexone procedure for calcium in serum Clin. Chem. 20 1458.
[16]. N. W. Tietz 1986 Textbook of Clinical Chemistry WB Saunders Company Philadelphia 775–1392.
[17]. P. Fossati L. Prencipe G. Berti 1980 Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine Clin. Chem. 26 227–231.
[18]. P. Trinder 1969 Determination of blood glucose using an oxidaseperoxidase system with a noncarcinogenic chromogen J. Clin. Path. 22 158–161.
[19]. S. Fiala A. E. Fiala B. Dixon 1972 Gamma glutamyl transpeptidase in transplantable chemically induced rat hepatomas and spontaneous mouse hepatomas J. Nat. Cancer Inst. 48 1393–1409.
[20]. H. C. Utley F. Bernheim P. Hochslein 1967 Effect of sulfhydryl reagent on peroxidation in microsome Arch. Biochem. Biophys. 260 521–531.
[21]. J. Sedlak R. H. Lindsay 1968 Estimation of total, protein bound & non-protein SH groups in tissue with Ellman's reagent Anal. Biochem. 25 192–205.
[22]. H. Murakami K. Yayama J. Chao L. Chao 1999 Atrial natriuretic peptide gene delivery attenuates gentamycininduced nephrotoxicity in rats Nephrol. Dial. Transplant. 14 1376–1384.
[23]. J. Karahan A. Atessahin S. Yilmaz A. O. Ceribasi F. Sakin 2005 Protective effect of lycopene on gentamicininduced oxidative stress and nephrotoxicity in rats Toxicology 215 198–204.
[24]. O. A. Al-Shabanah A. M. Aleisa A. A. Al-Yahya S. S. Al-Rejaie S. A. Bakheet A. G. Fatani 2010 Increased urinary losses of carnitine and decreased intramitochondrial coenzyme A in gentamicin-induced acute renal failure in rats Nephrol. Dial. Transplant. 25 69–76.
[25]. F. Romero M. Perez M. Chavez G. Parra P. Durante 2009 Effect of uric acid on gentamicin-induced nephrotoxicity in rats-role of matrix metalloproteinases 2 and 9 Basic Clin. Pharmacol. Toxicol. 105 416–424.
[26]. C. Silan O. Uzun N. U. Comunoglu S. Gokcen S. Bedirhan M. Cengiz 2007 Gentamicin induced nephrotoxicity in rats ameliorated and healing effects of resveratrol Biol. Pharm. Bull. 30 79–83.
[27]. G. Bledsoe S. Crickman J. Mao C. F. Xia H. Murakami L. Chao 2006 Kallikrein/kinin protects against gentamicin-induced nephrotoxicity by inhibition of inflammation and apoptosis Nephrol. Dial. Transplant. 21 624–633.
[28]. I. T. Abdel-Raheem G. A. El-Sherbiny A. Taye 2010 Green tea ameliorates renal oxidative damage induced by gentamicin in rats Pak. J. Pharm. Sci. 23 21–28.
[29]. B. V. S. Lakshmi M. Sudhakar 2010 Protective effect of Zingiber officinale on gentamicin induced nephrotoxicity in rats Int. J. Pharmacol. 6 58–62.
[30]. M. Kadkhodaee H. Khastar M. Faghihi R. Ghaznavi M. Zahmatkesh 2005 Effects of co-supplementation of vitamins E and C on gentamicin-induced nephrotoxicity in rat Exp. Physiol. 90 571–576.
[31]. N. Kaul N. Siveski-Iliskovic M. Hill J. Slezak P. K. Singal 1993 Free radicals and the heart J. Pharmacol. Toxicol. Methods 30 55–67.
[32]. I. T. Abdel-Raheem A. A. Abdel-Ghany G. A. Mohamed 2009 Protective effects of Quercetin against gentamicininduced nephrotoxicity in rats Biol. Pharm. Bull. 32 61–67.
[33]. I. Yaman E. Balikci 2010 Protective effects of Nigella sativa against gentamicin-induced nephrotoxicity in rats Exp. Toxicol. Pathol. 62 183–190.
[34]. S. Priyamrada M. Priyadarshini N. A. Arivarasu N. Farooq S. Khan S. A. Khan 2008 Studies on the protective effect of dietary fish oil on gentamicin-induced nephrotoxicity and oxidative damage in rat kidney Prostaglandins Leukot. Essent. Fatty Acids 78 369–381.
[35]. A. Kuhad N. Tirkey S. Pilkhwal K. Chopra 2006 Effect of spirulina, a blue green algae, on gentamicin-induced oxidative stress and nephrotoxicity in rats Fundam. Clin. Pharmacol. 20 121–128.
[36]. A. T. Diplock 1994 Antioxidants and free radicals scavengers C. A. Rice-Evans R. H. Burdon Free Radical Damage and Its Control Elsevier Science Amsterdam 43–49.
[37]. G. V. Harlalka C. R. Patil M. R. Patil 2007 Protective effect of Kalanchoe pinnata pers. (Crassulaceae) on gentamicin-induced nephrotoxicity in rats Indian J. Pharmacol. 39 201–205.
[38]. S. A. Khan S. Priyamvada N. Farooq S. Khan M. W. Khan N. K. Yusufi 2009 Protective effect of green tea extract on gentamicin-induced nephrotoxicity and oxidative damage in rat kidney Pharmacol. Res. 59 254–262.
[39]. B. C. Koti P. Ashok 2010 Diuretic activity of extracts of Mimusops olengi Linn Bark. Int. J. Green Pharm. 4 90–92.
[40]. B. Nitha K. K. Janardhanan 2008 Aqueous-ethanolic extract of morel mushroom mycelium Morchella esculenta, protects cisplatin and gentamicin induced nephrotoxicity in mice Food Chem. Toxicol. 46 3193–3199.
[41]. A. Shirwaikar D. Issac S. Malini 2004 Effect of Aerva lanata cisplatin and gentamicin models of acute renal failure J. Ethnopharmacol. 90 81–86.