View More View Less
  • 1 Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh, 11451, Saudi Arabia
Restricted access

Abstract

In this study, it was aimed to investigate the possible protective effect of an ethanolic extract of Basella alba L. on gentamycin (GM)-induced nephrotoxicity in Wistar albino rats. In the toxic group, rats were administered GM only (100 mg/kg, i.p.) for 8 days. In drug treated groups, rats were pretreated with B. alba (250 and 500 mg/kg per day orally) for 14 days and co-treated with GM for 8 days. After 24 h of the last dose, blood, urine, and tissue samples were collected from the animals. GM when administered induced a marked renal failure, characterized by a significant increase in serum and urine creatinine, urea, uric acid, gamma-glutamyl transferase (GGT), and protein levels. Besides, there were elevation of malondialdehyde (MDA) level and decrease in the concentration of total proteins (TPs) and free -SH in kidney tissue, which are indicators of oxidative stress of kidney. The extract also significantly reduced the GM-induced elevated serum and urine levels of sodium, potassium, calcium, protein, creatinine, urea, uric acid, and GGT. The tissue MDA level was also significantly diminished; the decreased free -SH and TP levels were significantly replenished by an ethanolic extract of B. alba treatment. The experimental results suggest that B. alba extract protected GM-induced nephrotoxicity, possibly by enhancing renal antioxidant system. The findings suggest the potential therapeutic use of B. alba as a novel nephroprotective agent.

  • [1]. C. Martínez-Salgado F. J. Lopez-Hernandez J. M. López-Novoa 2007 Glomerular nephrotoxicity of aminoglycosides Toxicol. Appl. Pharmacol. 223 8698.

    • Search Google Scholar
    • Export Citation
  • [2]. E. Arias R. N. Anderson H. C. Kung S. L. Murphy K. D. Kochanek 2003 Deaths: final data for 2001 Natl. Vital Stat Rep. 52 111115.

    • Search Google Scholar
    • Export Citation
  • [3]. P. Singh M. M. Srivastava L. D. Khemani 2009 Renoprotective effects of Andrographis paniculata (Burm. F.) Nees in rats Ups. J. Med. Sci. 114 136139.

    • Search Google Scholar
    • Export Citation
  • [4]. A. A. Al-Qarawi H. A. Rahman H. M. Mousa B. H. Ai S. A. El-Mougry 2008 Nephroprotective action of Phoenix dactylifera in gentamicin induced nephrotoxicity Pharmaceut. Biol. 46 227230.

    • Search Google Scholar
    • Export Citation
  • [5]. G. V. Harlalka C. R. Patil M. R. Patil 2007 Protective effect Kalanchoe pinnata pers. (Crassulaceae) on gentamicin induced nephrotoxicity in rats Indian J. Pharmacol. 39 201205.

    • Search Google Scholar
    • Export Citation
  • [6]. W. Dymock C. J. H. Warden D. Hooper 1890 Pharmacographia Indica: A History of the Principal Drugs of Vegetable Origin, Met with in British India Kegan Paul, Trench, Trübner & Co. Ltd. London 367.

    • Search Google Scholar
    • Export Citation
  • [7]. L. M. Perry 1980 Medicinal Plants of East and Southeast Asia: Attributed Properties and Uses The MIT Press Cambridge, Massachusetts 54.

    • Search Google Scholar
    • Export Citation
  • [8]. J. A. Duke E. S. Ayensu 1984 Medicinal Plants of China Reference Publications, Inc. Algonac, Michigan.

  • [9]. S. S. Hebba V. H. Harsha V. Shripathi G. R. Hegde 2004 Ethnomedicine of Dharwad district in Karnataka, India — Plants used in oral health care J. Ethnopharmacol. 94 261266.

    • Search Google Scholar
    • Export Citation
  • [10]. G. C. Yen H. Y. Chen H. H. Peng 2001 Evaluation of the chytotoxicity, mutagenecity and antimutagenecity of emerging edible plants Food Chem. Toxicol. 39 10451053.

    • Search Google Scholar
    • Export Citation
  • [11]. G. D. Reddy R. Kartik Ch. V. Rao M. K. Unnikrishnan P. Pushpangadan 2008 Basella alba extract act as antitumor and antioxidant potential against N-nitrosodiethylamine induced hepatocellular carcinoma Int. J. Infect. Dis. 12 S3 S68.

    • Search Google Scholar
    • Export Citation
  • [12]. N. R. Fransworth 1966 Biological and phytochemical screening of plants J. Pharm. Sci. 55 225272.

  • [13]. Organization for Economic Cooperation and Development (OECD): OECD Guidelines for Testing of Chemicals. Acute Oral Toxicity, OECD (1993) No. 401.

    • Search Google Scholar
    • Export Citation
  • [14]. T. Jeyanthi P. Subramanian 2009 Nephroprotective effect of Withania somnifera: a dose-dependent stu dy Ren. Fail. 31 814821.

  • [15]. W. R. Moorhead H. C. Briggs 1974 2-Amino-2-methyl-1-propanol as the alkalizing agent in an improved continuous-flow cresolphthalein complexone procedure for calcium in serum Clin. Chem. 20 1458.

    • Search Google Scholar
    • Export Citation
  • [16]. N. W. Tietz 1986 Textbook of Clinical Chemistry WB Saunders Company Philadelphia 7751392.

  • [17]. P. Fossati L. Prencipe G. Berti 1980 Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine Clin. Chem. 26 227231.

    • Search Google Scholar
    • Export Citation
  • [18]. P. Trinder 1969 Determination of blood glucose using an oxidaseperoxidase system with a noncarcinogenic chromogen J. Clin. Path. 22 158161.

    • Search Google Scholar
    • Export Citation
  • [19]. S. Fiala A. E. Fiala B. Dixon 1972 Gamma glutamyl transpeptidase in transplantable chemically induced rat hepatomas and spontaneous mouse hepatomas J. Nat. Cancer Inst. 48 13931409.

    • Search Google Scholar
    • Export Citation
  • [20]. H. C. Utley F. Bernheim P. Hochslein 1967 Effect of sulfhydryl reagent on peroxidation in microsome Arch. Biochem. Biophys. 260 521531.

    • Search Google Scholar
    • Export Citation
  • [21]. J. Sedlak R. H. Lindsay 1968 Estimation of total, protein bound & non-protein SH groups in tissue with Ellman's reagent Anal. Biochem. 25 192205.

    • Search Google Scholar
    • Export Citation
  • [22]. H. Murakami K. Yayama J. Chao L. Chao 1999 Atrial natriuretic peptide gene delivery attenuates gentamycininduced nephrotoxicity in rats Nephrol. Dial. Transplant. 14 13761384.

    • Search Google Scholar
    • Export Citation
  • [23]. J. Karahan A. Atessahin S. Yilmaz A. O. Ceribasi F. Sakin 2005 Protective effect of lycopene on gentamicininduced oxidative stress and nephrotoxicity in rats Toxicology 215 198204.

    • Search Google Scholar
    • Export Citation
  • [24]. O. A. Al-Shabanah A. M. Aleisa A. A. Al-Yahya S. S. Al-Rejaie S. A. Bakheet A. G. Fatani 2010 Increased urinary losses of carnitine and decreased intramitochondrial coenzyme A in gentamicin-induced acute renal failure in rats Nephrol. Dial. Transplant. 25 6976.

    • Search Google Scholar
    • Export Citation
  • [25]. F. Romero M. Perez M. Chavez G. Parra P. Durante 2009 Effect of uric acid on gentamicin-induced nephrotoxicity in rats-role of matrix metalloproteinases 2 and 9 Basic Clin. Pharmacol. Toxicol. 105 416424.

    • Search Google Scholar
    • Export Citation
  • [26]. C. Silan O. Uzun N. U. Comunoglu S. Gokcen S. Bedirhan M. Cengiz 2007 Gentamicin induced nephrotoxicity in rats ameliorated and healing effects of resveratrol Biol. Pharm. Bull. 30 7983.

    • Search Google Scholar
    • Export Citation
  • [27]. G. Bledsoe S. Crickman J. Mao C. F. Xia H. Murakami L. Chao 2006 Kallikrein/kinin protects against gentamicin-induced nephrotoxicity by inhibition of inflammation and apoptosis Nephrol. Dial. Transplant. 21 624633.

    • Search Google Scholar
    • Export Citation
  • [28]. I. T. Abdel-Raheem G. A. El-Sherbiny A. Taye 2010 Green tea ameliorates renal oxidative damage induced by gentamicin in rats Pak. J. Pharm. Sci. 23 2128.

    • Search Google Scholar
    • Export Citation
  • [29]. B. V. S. Lakshmi M. Sudhakar 2010 Protective effect of Zingiber officinale on gentamicin induced nephrotoxicity in rats Int. J. Pharmacol. 6 5862.

    • Search Google Scholar
    • Export Citation
  • [30]. M. Kadkhodaee H. Khastar M. Faghihi R. Ghaznavi M. Zahmatkesh 2005 Effects of co-supplementation of vitamins E and C on gentamicin-induced nephrotoxicity in rat Exp. Physiol. 90 571576.

    • Search Google Scholar
    • Export Citation
  • [31]. N. Kaul N. Siveski-Iliskovic M. Hill J. Slezak P. K. Singal 1993 Free radicals and the heart J. Pharmacol. Toxicol. Methods 30 5567.

    • Search Google Scholar
    • Export Citation
  • [32]. I. T. Abdel-Raheem A. A. Abdel-Ghany G. A. Mohamed 2009 Protective effects of Quercetin against gentamicininduced nephrotoxicity in rats Biol. Pharm. Bull. 32 6167.

    • Search Google Scholar
    • Export Citation
  • [33]. I. Yaman E. Balikci 2010 Protective effects of Nigella sativa against gentamicin-induced nephrotoxicity in rats Exp. Toxicol. Pathol. 62 183190.

    • Search Google Scholar
    • Export Citation
  • [34]. S. Priyamrada M. Priyadarshini N. A. Arivarasu N. Farooq S. Khan S. A. Khan 2008 Studies on the protective effect of dietary fish oil on gentamicin-induced nephrotoxicity and oxidative damage in rat kidney Prostaglandins Leukot. Essent. Fatty Acids 78 369381.

    • Search Google Scholar
    • Export Citation
  • [35]. A. Kuhad N. Tirkey S. Pilkhwal K. Chopra 2006 Effect of spirulina, a blue green algae, on gentamicin-induced oxidative stress and nephrotoxicity in rats Fundam. Clin. Pharmacol. 20 121128.

    • Search Google Scholar
    • Export Citation
  • [36]. A. T. Diplock 1994 Antioxidants and free radicals scavengers C. A. Rice-Evans R. H. Burdon Free Radical Damage and Its Control Elsevier Science Amsterdam 4349.

    • Search Google Scholar
    • Export Citation
  • [37]. G. V. Harlalka C. R. Patil M. R. Patil 2007 Protective effect of Kalanchoe pinnata pers. (Crassulaceae) on gentamicin-induced nephrotoxicity in rats Indian J. Pharmacol. 39 201205.

    • Search Google Scholar
    • Export Citation
  • [38]. S. A. Khan S. Priyamvada N. Farooq S. Khan M. W. Khan N. K. Yusufi 2009 Protective effect of green tea extract on gentamicin-induced nephrotoxicity and oxidative damage in rat kidney Pharmacol. Res. 59 254262.

    • Search Google Scholar
    • Export Citation
  • [39]. B. C. Koti P. Ashok 2010 Diuretic activity of extracts of Mimusops olengi Linn Bark. Int. J. Green Pharm. 4 9092.

  • [40]. B. Nitha K. K. Janardhanan 2008 Aqueous-ethanolic extract of morel mushroom mycelium Morchella esculenta, protects cisplatin and gentamicin induced nephrotoxicity in mice Food Chem. Toxicol. 46 31933199.

    • Search Google Scholar
    • Export Citation
  • [41]. A. Shirwaikar D. Issac S. Malini 2004 Effect of Aerva lanata cisplatin and gentamicin models of acute renal failure J. Ethnopharmacol. 90 8186.

    • Search Google Scholar
    • Export Citation

Clinical and Experimental Medical Journal
Language English
Size  
Year of
Foundation
2007
Publication
Programme
ceased
Volumes
per Year
 
Issues
per Year
 
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 2060-6249 (Print)
ISSN 2060-968X (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
May 2021 14 0 0
Jun 2021 13 0 0
Jul 2021 7 0 0
Aug 2021 6 0 0
Sep 2021 1 0 0
Oct 2021 9 0 0
Nov 2021 0 0 0