Authors:
Szaniszló Z. Jávor Department of Surgical Research and Techniques, Faculty of Medicine, Pécs, Hungary

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K. Shanava Department of Surgical Research and Techniques, Faculty of Medicine, Pécs, Hungary

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E. Hocsák Departments of Biochemistry and Medical Chemistry, Faculty of Medicine, Pécs, Hungary

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M. Kürthy Department of Surgical Research and Techniques, Faculty of Medicine, Pécs, Hungary

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J. Lantos Department of Surgical Research and Techniques, Faculty of Medicine, Pécs, Hungary

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B. Borsiczky Department of Surgical Research and Techniques, Faculty of Medicine, Pécs, Hungary

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I. Takács Department of Surgical Research and Techniques, Faculty of Medicine, Pécs, Hungary

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Sz. Horváth Department of Surgical Research and Techniques, Faculty of Medicine, Pécs, Hungary

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B. Balatonyi Department of Surgical Research and Techniques, Faculty of Medicine, Pécs, Hungary

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S. Ferencz Department of Surgical Research and Techniques, Faculty of Medicine, Pécs, Hungary

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A. Ferencz Department of Surgical Research and Techniques, Faculty of Medicine, Pécs, Hungary

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E. Rőth Department of Surgical Research and Techniques, Faculty of Medicine, Pécs, Hungary

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Gy. Wéber Department of Surgical Research and Techniques, Faculty of Medicine, Pécs, Hungary

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Abstract

Increased intra-abdominal pressure during laparoscopy leads to hypoxia due to reduced blood flow. Aim of our study was to investigate whether preconditioning can reduce this negative effect of the pneumoperitoneum. Fifty female Wistar rats were used, divided into 5 groups. I: Sham operation (Sham), II: conventional pneumoperitoneum (PP), III: transvaginal pneumoperitoneum (TV), IV: preconditioning for 2.5 minutes in two cycles (Pre 2.5), V: preconditioning for 5 minutes (Pre 5). Malondialdehyde (MDA), reduced glutathione (GSH), sulfhydrylgroup (SH-) concentrations, superoxide-dismutase (SOD) and mieloperoxidase (MPO) activity, and anti-apoptotic pathway marker p-AKT level and inflammatory cytokine TNF-α were measured. SOD activity and GSH concentration were decreased in PP and TV groups comparing to Sham and preconditioning groups. MPO activity was decreased also in PP and TV groups comparing to the Sham group but in the preconditioning groups it has remained high. MDA concentration in plasma was increased in PP and TV groups comparing to Sham and preconditioning groups. There was no difference in the case of blood MDA and SH-concentrations between groups. Anti-apoptotic pathway marker p-AKT level was decreased in the TV group comparing to the sham and preconditioning groups. TNF-α level was increased in TV and preconditioning groups compared to the sham group. According to the results preconditioning can reduce negative effects of pneumoperitoneum.

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2019  
Scimago
H-index
11
Scimago
Journal Rank
0,220
Scimago
Quartile Score
Medicine (miscellaneous) Q3
Scopus
Cite Score
155/133=1,2
Scopus
Cite Score Rank
General Medicine 199/529 (Q2)
Scopus
SNIP
0,343
Scopus
Cites
206
Scopus
Documents
23

 

Interventional Medicine and Applied Science
Language English
Size  
Year of
Foundation
2009
Publication
Programme
changed title
Volumes
per Year
 
Issues
per Year
 
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 2061-1617 (Print)
ISSN 2061-5094 (Online)

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