View More View Less
  • 1 Hungarian Natural History Museum Ludovika tér 2-6 H-1083 Budapest Hungary
  • | 2 Hungarian Academy of Sciences Institute of Ecology and Botany Vácrátót Hungary
Restricted access

We studied the intrapopulational genetic structure of a small rock fern subspecies Asplenium ceterach ssp. ceterach living in a fragmented habitat. The level and distribution of genetic variation are primarily affected by the colonization events, the reproductive properties of the species, and the short-distance (leptokurtic) dispersal of spores. These biological processes are at the same time strongly influenced by the geomorphological heterogeneity of the habitat. The individuals’ genetic variation was detected as multilocus RAPD profile. The spatially constrained portion of genetic variance within subpopulations was studied by means of variograms, i.e., the average genetic dissimilarity between the pairs of organisms against the separation distance. The range of reduced dissimilarity was 11–13 m. Mantel test proved that subpopulations are isolated by distance. The connected local population patches may create a metapopulation structure. This study also surveyed the adequacy of RAPD products to detect the low level of genetic variation of a polyploid plant population.

  • Barrington, D. 1993. Ecological and historical factors in fern biogeography. J. Phylogeogr. 20: 275–280.

    Barrington D. , 'Ecological and historical factors in fern biogeography ' (1993 ) 20 J. Phylogeogr. : 275 -280.

    • Search Google Scholar
  • Bauer, D. F. 1972. Constructing confidence sets using rank statistics. J. Amer. Stat. Assoc. 67:687–690.

    Bauer D. F. , 'Constructing confidence sets using rank statistics ' (1972 ) 67 J. Amer. Stat. Assoc. : 687 -690.

    • Search Google Scholar
  • Benecke, M. 1998. Random amplified polymorphic DNS (RAPD) typing of necrophageous (diptera, coleoptera) in criminal forensic studies: validation and use in practice. Forensic Sci. International 98: 157–168.

    Benecke M. , 'Random amplified polymorphic DNS (RAPD) typing of necrophageous (diptera, coleoptera) in criminal forensic studies: validation and use in practice ' (1998 ) 98 Forensic Sci. International : 157 -168.

    • Search Google Scholar
  • Copeland, E.B. 1947. Genera Filicum. Waltham, Mass.

    Copeland E.B. , '', in Genera Filicum , (1947 ) -.

  • Dray, S. and A.B. Dufour. 2007. The ade4 package: implementing the duality diagram for ecologists. J. Stat. Software 22: 1–20.

    Dufour A.B. , 'The ade4 package: implementing the duality diagram for ecologists ' (2007 ) 22 J. Stat. Software : 1 -20.

    • Search Google Scholar
  • Fortin, M.J., M.R.T. Dale, and J. ver Hoef. 2002. Spatial analysis in ecology. Encyclopedia of Envirometrics 4: 2051–2058.

    Hoef J. , 'Spatial analysis in ecology ' (2002 ) 4 Encyclopedia of Envirometrics : 2051 -2058.

    • Search Google Scholar
  • Franklin, R.B., L.K. Blum, A.C. McComb, A.L. Mills. 2002. A geostatistical analysis of small-scale spatial variability in bacterial abundance and community structure in salt march creek bank sediments. FEMS Microbiol. Ecol. 42: 71–80.

    Mills A.L. , 'A geostatistical analysis of small-scale spatial variability in bacterial abundance and community structure in salt march creek bank sediments ' (2002 ) 42 FEMS Microbiol. Ecol. : 71 -80.

    • Search Google Scholar
  • Gupta, P., K. 2007. Genetics: Classical to Modern. Capital Offset Press.

  • Grivet, D. and R.J. Petit. 2003. Chloroplast DNA phylogeography of the hornbeam in Europe: Evidence for a bottleneck at a the outset of postglacial colonization. Conserv. Gen. 4: 47–56.

    Petit R.J. , 'Chloroplast DNA phylogeography of the hornbeam in Europe: Evidence for a bottleneck at a the outset of postglacial colonization ' (2003 ) 4 Conserv. Gen. : 47 -56.

    • Search Google Scholar
  • Hansen, M., C. Halldén and T. Säll. 1998. Error rates and polymorphism frequencies for three RAPD protocols. Plant Molecular Biology Reporter 16: 139–146.

    Säll T. , 'Error rates and polymorphism frequencies for three RAPD protocols ' (1998 ) 16 Plant Molecular Biology Reporter : 139 -146.

    • Search Google Scholar
  • Klekowski, E. 1979. The genetics and reproductive biology of ferns. In: A.F. Dyer (ed.), The Experimental Biology of Ferns. Academic Press London. pp. 133–170.

  • KrÍzsik, V., I. Pinter, Á. Major and G. Vida. 2007. Spatial pattern of individual genetic similarities in population of Asplenium ceterach (Aspleniaceae: Pteridophyta). Fern Gazette 18: 59–70.

    Vida G. , 'Spatial pattern of individual genetic similarities in population of Asplenium ceterach (Aspleniaceae: Pteridophyta) ' (2007 ) 18 Fern Gazette : 59 -70.

    • Search Google Scholar
  • Lamboy, W.F. 1994a. Computing genetic similarity coefficients from RAPD data: the effects of PCR artifacts. PCR Methods Applic. 4: 31–37.

    Lamboy W.F. , 'Computing genetic similarity coefficients from RAPD data: the effects of PCR artifacts ' (1994 ) 4 PCR Methods Applic. : 31 -37.

    • Search Google Scholar
  • Lamboy, W.F. 1994b. Computing genetic similarity coefficients from RAPD data: Correcting for the effects of PCR artifacts caused by variation in experimental conditions. PCR Methods Applic. 4: 38–43.

    Lamboy W.F. , 'Computing genetic similarity coefficients from RAPD data: Correcting for the effects of PCR artifacts caused by variation in experimental conditions ' (1994 ) 4 PCR Methods Applic. : 38 -43.

    • Search Google Scholar
  • Liu, Z.J., P. Li, B.J. Argue and R.A. Dunham. 1998. Random amplified polymorphic DNA markers: usefulness for gene mapping and analysis of genetic variation of catfish. Aquaculture 174: 59–68.

    Dunham R.A. , 'Random amplified polymorphic DNA markers: usefulness for gene mapping and analysis of genetic variation of catfish ' (1998 ) 174 Aquaculture : 59 -68.

    • Search Google Scholar
  • Manton, I. 1950. Problems of Cytology and Evolution in the Pteridophyta. Cambridge University Press, Cambridge.

    Manton I. , '', in Problems of Cytology and Evolution in the Pteridophyta , (1950 ) -.

  • Molnár, G. and G. Timár. 2002. Az EOV — koordináták nagypontosságú közelítése Hotine-féle ferdetengelyû Mercator-vetülettel (in Hungarian with English summary). Geodézia és Kartográfia 54: 18–22.

    Timár G. , 'Az EOV — koordináták nagypontosságú közelítése Hotine-féle ferdetengelyû Mercator-vetülettel (in Hungarian with English summary) ' (2002 ) 54 Geodézia és Kartográfia : 18 -22.

    • Search Google Scholar
  • Moran, R.C. 2007. Introduction to papers from the Fern Biogeography Symposium, International Botanical Congress, 2005. Brittonia 59: 107.

    Moran R.C. , 'Introduction to papers from the Fern Biogeography Symposium, International Botanical Congress, 2005 ' (2007 ) 59 Brittonia : 107 -.

    • Search Google Scholar
  • Nei, M. and W.H. Li. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. 76: 5269–5273.

    Li W.H. , 'Mathematical model for studying genetic variation in terms of restriction endonucleases ' (1979 ) 76 Proc. Natl. Acad. Sci. : 5269 -5273.

    • Search Google Scholar
  • Pebesma, E.J. 2004. Multivariable geostatistics in S: the gstat package. Computers & Geosciences 30: 683–691

    Pebesma E.J. , 'Multivariable geostatistics in S: the gstat package ' (2004 ) 30 Computers & Geosciences : 683 -691.

    • Search Google Scholar
  • Pintér, I. 1995. Progeny studies of the fern hybrid Polystichum x biknellii (Dryopteridaceae: Pteridophyta). Fern Gazette 15: 25–50.

    Pintér I. , 'Progeny studies of the fern hybrid Polystichum x biknellii (Dryopteridaceae: Pteridophyta) ' (1995 ) 15 Fern Gazette : 25 -50.

    • Search Google Scholar
  • Pintér, I., F. Bakker, J. Barrett, C. Cox, M. Gibby, S. Henderson, M. Morgan-Richards, F. Rumsey, S. Russell, S. Trewick, H. Schneider and J. Vogel. 2002. Phylogenetic and biosystematic relationships in four disjunct polyploid complexes in the subgenera Ceterach and Phyllitis in Asplenium (Aspleniaceae). Organism, Diversity and Evolution 2: 299–311.

    Vogel J. , 'Phylogenetic and biosystematic relationships in four disjunct polyploid complexes in the subgenera Ceterach and Phyllitis in Asplenium (Aspleniaceae) ' (2002 ) 2 Organism, Diversity and Evolution : 299 -311.

    • Search Google Scholar
  • Reichstein, T. 1981. Hybrids in European Aspleniaceae (Pteridophyta). Bot. Helv. 91: 89–139.

    Reichstein T. , 'Hybrids in European Aspleniaceae (Pteridophyta) ' (1981 ) 91 Bot. Helv. : 89 -139.

    • Search Google Scholar
  • Rodrigues, F.M., J.A.F. Diniz-Filho, L.A.M. Bataus and R.P. Batos. 2002. Hypothesis testing of genetic similarity based on RAPD data using Mantel tests and model matrices. Gen. Mol. Biol.25: 435–438.

    Batos R.P. , 'Hypothesis testing of genetic similarity based on RAPD data using Mantel tests and model matrices ' (2002 ) 25 Gen. Mol. Biol. : 435 -438.

    • Search Google Scholar
  • Sauer, P., M. Müller and J. Kang. 1998. Quantitation of DNA. Qiagen News 2: 23–26.

    Kang J. , 'Quantitation of DNA ' (1998 ) 2 Qiagen News : 23 -26.

  • Schmitt, T. 2007. Molecular biogeography of Europe: Pleistocene cycles and postglacial trends. Frontiers in Zool. 4:11.

    Schmitt T. , 'Molecular biogeography of Europe: Pleistocene cycles and postglacial trends ' (2007 ) 4 Frontiers in Zool. : 11 -.

    • Search Google Scholar
  • Schneller, J., R. Holderegger, F. Gugerli, K. Eichenberger and E. Lutz. 1998. Patterns of genetic variation detected by RAPDs suggest a single origin with subsequent mutations and long-distance dispersal in the apomictic fern Dryopteris remota. Amer. J. Bot. 85: 1038–1042.

    Lutz E. , 'Patterns of genetic variation detected by RAPDs suggest a single origin with subsequent mutations and long-distance dispersal in the apomictic fern Dryopteris remota ' (1998 ) 85 Amer. J. Bot. : 1038 -1042.

    • Search Google Scholar
  • Sokal, R.R. and C.D. Michener. 1958. A statistical method for evaluating systematic relationships. Univ. Kansas Sci. Bull. 38:1409–1438.

    Michener C.D. , 'A statistical method for evaluating systematic relationships ' (1958 ) 38 Univ. Kansas Sci. Bull. : 1409 -1438.

    • Search Google Scholar
  • Soltis, D.E. and P.S. Soltis. 1999. Polyploidy: recurrent formation and genome evolution. Trends Ecol. Evol. 14: 348–352.

    Soltis P.S. , 'Polyploidy: recurrent formation and genome evolution ' (1999 ) 14 Trends Ecol. Evol. : 348 -352.

    • Search Google Scholar
  • Stewart, C.N. and D.M. Porter. 1995. RAPD profiling in biological conservation: an application to estimating clonal variation in rare and endangered Iliamna in Virginia. Biol. Conserv. 74: 135–142.

    Porter D.M. , 'RAPD profiling in biological conservation: an application to estimating clonal variation in rare and endangered Iliamna in Virginia ' (1995 ) 74 Biol. Conserv. : 135 -142.

    • Search Google Scholar
  • Suter, M.; J.J. Schneller and J.C. Vogel. 2000. Investigations into the genetic variation, population structure, and breeding systems of fern Asplenium trichomanes subsp. quadrivalens. International J. Plant Sci. 161: 233–244.

    Vogel J.C. , 'Investigations into the genetic variation, population structure, and breeding systems of fern Asplenium trichomanes subsp. quadrivalens ' (2000 ) 161 International J. Plant Sci. : 233 -244.

    • Search Google Scholar
  • Timár, G. and G. Molnár 2002. A HD72?ETRS89 transzformáció szabványosítási problémái (in Hungarian with English summary). Geodézia és Kartográfia 54: 28–30.

    Molnár G. , 'A HD72?ETRS89 transzformáció szabványosítási problémái (in Hungarian with English summary) ' (2002 ) 54 Geodézia és Kartográfia : 28 -30.

    • Search Google Scholar
  • Trewick, S.A., M. Morgan-Richards, S.J. Russell, F. Henderson, F.J. Rumsey, I. Pintér, J.A. Barrett, M. Gibby and J.C. Vogel. 2002. Polyploidy, phylogeography and Pleistocene refugia of the rockfern Asplenium ceterach: evidence from chloroplast DNA. Mol. Ecol. 11: 2003–2012.

    Vogel J.C. , 'Polyploidy, phylogeography and Pleistocene refugia of the rockfern Asplenium ceterach: evidence from chloroplast DNA ' (2002 ) 11 Mol. Ecol. : 2003 -2012.

    • Search Google Scholar
  • Van Den Heede, C. J., R.L.L. Viane and M. Chase. 2003. Phylogenetic analysis of Asplenium subgenus Ceterach (Pteridophyta: Aspleniaceae) based on plastid and nuclear ribosomal ITS DNA sequences. Amer. J. Bot. 90: 481–495.

    Chase M. , 'Phylogenetic analysis of Asplenium subgenus Ceterach (Pteridophyta: Aspleniaceae) based on plastid and nuclear ribosomal ITS DNA sequences ' (2003 ) 90 Amer. J. Bot. : 481 -495.

    • Search Google Scholar
  • Van Den Heede, C. J., S. Pajarón, E. Pangua and R.L.L. Viane. 2004. Asplenium ceterach and A. octoploideum on the Canary Islands (Aspleniaceae, Pteridophyta). Amer. Fern J. 94:81–111.

    Viane R.L.L. , 'Asplenium ceterach and A. octoploideum on the Canary Islands (Aspleniaceae, Pteridophyta) ' (2004 ) 94 Amer. Fern J. : 81 -111.

    • Search Google Scholar
  • Viane, R.L.L., A.C. Jermy and J.D. Lovis. 1993. Asplenium. In: T.G. Tutin, N.A. Burges, A.O. Chater, J.R. Edmondson, V.H. Heywood, D.M. Moore, D.H. Valentine, S.M. Walters and D.A. Webb (eds.), Flora Europea, 2nd ed., Cambridge University Press, Cambridge. Vol. 1, pp. 18–23.

    Lovis J.D. , '', in Flora Europea , (1993 ) -.

  • Vida, G. 1963. A new Asplenium (sectio ceterach) species and the problem of the origin of Phyllitis hybrida (Milde) C. Christ. Acta Bot. Acad. Sci. Hung. 9:197–215.

    Vida G. , 'A new Asplenium (sectio ceterach) species and the problem of the origin of Phyllitis hybrida (Milde) C. Christ ' (1963 ) 9 Acta Bot. Acad. Sci. Hung. : 197 -215.

    • Search Google Scholar
  • Vida, G. 1965. A magyarországi páfrányok (Filicidae) cytotaxonómiája. (Cytotaxonomy of ferns (Filicidae) in Hungary). Dissertation, Budapest.

  • Vogel, J.C., F.J. Rumsey, S. Russell, C.J. Cox, J.S. Holmes, W. Bujnoch, C. Stark, A. Barrett and M. Gibby. 1999a. Genetic structure, reproductive biology and ecology of isolated populations of Asplenium csikii (Aspleniaceae, Pteridophyta). Heredity 83: 604–612.

    Gibby M. , 'Genetic structure, reproductive biology and ecology of isolated populations of Asplenium csikii (Aspleniaceae, Pteridophyta) ' (1999 ) 83 Heredity : 604 -612.

    • Search Google Scholar
  • Vogel, J.C., F.J. Rumsey, J.J. Schneller, J.A Barrett and M. Gibby. 1999b. Where are the glacial refugia in Europe? Evidence from pteridophytes. Biol. J. Linnean Soc. 66: 23–37.

    Gibby M. , 'Where are the glacial refugia in Europe? Evidence from pteridophytes ' (1999 ) 66 Biol. J. Linnean Soc. : 23 -37.

    • Search Google Scholar
  • Webster, R. 1985. Quantitative spatial analysis of soil in the field. Adv. Soil Sci. 3: 1–70.

    Webster R. , 'Quantitative spatial analysis of soil in the field ' (1985 ) 3 Adv. Soil Sci. : 1 -70.

    • Search Google Scholar
  • Whitley, E. and J. Ball. 2002. Statistics review 6: Nonparametric methods. Critical Care 6: 509–513.

    Ball J. , 'Statistics review 6: Nonparametric methods ' (2002 ) 6 Critical Care : 509 -513.

  • Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski and S.V. Tingey. 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleid Acid Res. 18: 6531–6535.

    Tingey S.V. , 'DNA polymorphism amplified by arbitrary primers are useful as genetic markers ' (1990 ) 18 Nucleid Acid Res. : 6531 -6535.

    • Search Google Scholar
  • Wolf, P.G., H. Schneider and T.A. Ranker. 2001. Geographic distribution of homosporous ferns: does dispersal obscure evidence of vicariance? J. Biogeogr. 28: 263–270.

    Ranker T.A. , 'Geographic distribution of homosporous ferns: does dispersal obscure evidence of vicariance? ' (2001 ) 28 J. Biogeogr. : 263 -270.

    • Search Google Scholar