We tested the relationship between water chemical variables and macrophyte vegetation in an oxbow-lake of the Upper-Tisza, Hungary. There were 42 relevés in random plots of 2 m by 2 m and 20 chemical variables (Ca, Fe, Hydrogencarbonate, K, carbonate, Kjeldahl-nitrogen, chloride, COD(Cr), Mg, m-alkalinity, Mn, Na, NH4, NO2, NO3, dissolved orthophosphate-P, total phosphorus, pH, sulphate and conductivity) and a biological one (chlorophyll a) were measured. Detrended canonical correspondence analysis was used to explore the species-vegetation-water chemical variables relationship. Our results revealed that there were pronounced differences both in the vegetation and the chemical variables among the different kinds of vegetation patches. According to the DCCA, Trapetum natantis, Glycerietum maximae, Ceratophyllo-Nymphaeetum albae and Typhetum angustifoliae associations could be separated based on the relevés and environmental variables. Kjeldahl nitrogen and carbonate were found to be the most important variables. Our results suggest that water chemical variables had strong influence on vegetation development. The groups of relevés identified by the DCCA were coherent with classical phytosociological categories.