We studied biomass and species composition changes of open perennial sand grassland ( Festucetum vaginatae ) as response to different levels of simulated grazing pressures. We conducted a factorial micro-plot field experiment on previously grazed grassland that has been abandoned for a long time. In a two-way factorial design of 12 treatments × 8 repeats, we performed clipping (twice a year for three years) and litter treatments (removing and adding litter once at the beginning of the experiment) to simulate components of grazing, namely the biomass removal and the reduction of the litter accumulation. We used field spectroscopy and visual canopy cover estimation to measure the effects on the amount of the above-ground green biomass and on the vegetation composition.
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