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Anita Šalić University of Zagreb, Marulićev trg 19, HR-10000 Zagreb, Croatia

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Katarina Pindrić Xellia Ltd., Slavonska avenija 24/6, HR-10 000 Zagreb, Croatia

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Gordana Hojnik Podrepšek University of Maribor, Smetanova ulica 17, SI-2000 Maribor, Slovenia

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Nikolina Novosel University of Zagreb, Bijenička cesta 32, HR-10000 Zagreb, Croatia

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Maja Leitgeb University of Maribor, Smetanova ulica 17, SI-2000 Maribor, Slovenia

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Bruno Zelić University of Zagreb, Marulićev trg 19, HR-10000 Zagreb, Croatia

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In this study, magnetic nanoparticles (MNPs) of maghemite (γ-Fe2O3) were synthesized and characterized. The method of multifactor experimental design and evolutionary operation (EVOP) was used to optimize immobilization of the alcohol dehydrogenase (ADH) enzyme on MNPs. Optimal operating conditions for the immobilization process were determined (γADH = 0.08 mg/mL, 2% glutaraldehyde for surface activation, t = 28 h), and in such conditions, a specific activity of S.A. = 118 ± 6 U/mg and immobilization efficiency of η = 84.97 ± 3.67% were achieved. Compared to the native enzyme, ADH immobilized on MNPs retained 66.45 ± 3.66% of the initial activity. ADH immobilized on MNPs at optimal conditions was used as a biocatalyst for model reaction—NADH oxidation. NADH oxidation was performed in two different magnetic microreactor configurations: (1) microreactor equipped with permanent square magnets and (2) microreactor equipped with an electromagnet and an oscillating magnetic field that enables magnetic particles movement in the microreactor. In the system with the oscillating magnetic field, equal conversion (X = 100%) was achieved in 2-fold shorter residence time.

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Journal of Flow Chemistry
Language English
Size A4
Year of
Foundation
2011
Volumes
per Year
1
Issues
per Year
4
Founder Áramlásos Kémiai Tudományos Társaság
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Address
H-1031 Budapest, Hungary Záhony utca 7.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
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CH-6330 Cham, Switzerland Gewerbestrasse 11
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Publisher
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ISSN 2062-249X (Print)
ISSN 2063-0212 (Online)