Authors:
Babu Halan Helmholtz-Centre for Environmental Research — UFZ GmbH, Permoserstraße 15, 04318 Leipzig, Germany

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Rohan Karande Helmholtz-Centre for Environmental Research — UFZ GmbH, Permoserstraße 15, 04318 Leipzig, Germany

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Katja Buehler Helmholtz-Centre for Environmental Research — UFZ GmbH, Permoserstraße 15, 04318 Leipzig, Germany
TU Dortmund University, Emil-Figge-Str. 66, 44227, Dortmund, Germany

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Andreas Schmid Helmholtz-Centre for Environmental Research — UFZ GmbH, Permoserstraße 15, 04318 Leipzig, Germany

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This study investigated the survival and catalytic potential of a single species Pseudomonas taiwanensis VLB120ΔC biofilm for the conversion of styrene to (S)-styrene oxide in a multiphasic capillary microreactor containing the highly toxic substrate styrene as a pure phase. The catalytic biofilm was cultivated under high fluidic stress in a continuous three-phase segmented flow system comprising aqueous medium, air, and styrene. This concept required an adaptation period of 7 days, during which P. taiwanensis VLB120?C developed a biofilm exhibiting a remarkable cellular integrity with nearly 70% intact cells. In a three-phase segmented flow biofilm microreactor, an average specific styrene epoxidation rate of 10 g/Ltube/day was achieved continuously for a period of 20 days without any clogging problems. Overall, this note highlights the robustness of biofilms as a promising biocatalyst format for the conversion/synthesis of toxic organic chemicals and the applicability of multiphasic capillary microreactors for biofilm based catalysis.

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Journal of Flow Chemistry
Language English
Size A4
Year of
Foundation
2011
Volumes
per Year
1
Issues
per Year
4
Founder Áramlásos Kémiai Tudományos Társaság
Founder's
Address
H-1031 Budapest, Hungary Záhony utca 7.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 2062-249X (Print)
ISSN 2063-0212 (Online)