Journal of Flow Chemistry
Volume/Issue:
Volume 6: Issue 1
Microfluidic multiple cell chip reactor filled with enzyme-coated magnetic nanoparticles — An efficient and flexible novel tool for enzyme catalyzed biotransformations
Authors:
Ferenc Ender Budapest University of Technology and Economics, Budapest, Magyar tudósok körútja 2., H-1117 Budapest, Hungary

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Diána Weiser Budapest University of Technology and Economics, Műegyetem rkp. 3., H-1111 Budapest, Hungary

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Botond Nagy Babeş-Bolyai University of Cluj-Napoca, Arany János str. 11, Ro-400028 Cluj-Napoca, Romania

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Csaba László Bencze Babeş-Bolyai University of Cluj-Napoca, Arany János str. 11, Ro-400028 Cluj-Napoca, Romania

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Csaba Paizs Babeş-Bolyai University of Cluj-Napoca, Arany János str. 11, Ro-400028 Cluj-Napoca, Romania

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Péter Pálovics Budapest University of Technology and Economics, Budapest, Magyar tudósok körútja 2., H-1117 Budapest, Hungary

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László Poppe Budapest University of Technology and Economics, Műegyetem rkp. 3., H-1111 Budapest, Hungary
SynBiocat Ltd., H-1173 Budapest, Lázár deák u. 4/1.,, Hungary

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Pages:
43–52
Online Publication Date:
Mar 2016
Publication Date:
01 Mar 2016
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1846.2015.00036
Keywords:
magnetic nanoparticle; magnetic chip reactor; continuous-flow biotransformation; phenylalanine ammonia-lyase; unnatural amino acid
Restricted access

Biotransformation of l-phenylalanine (l-1a) and five unnatural substrates (rac-1b–f) by phenylalanine ammonia-lyase (PAL) was investigated in a novel microfluidic device (Magne-Chip) that comprises microliter volume reaction cells filled with PAL-coated magnetic nanoparticles (MNPs). Experiments proved the excellent reproducibility of enzymecatalyzed biotransformation in the chip and the excellent reusability of the enzyme layer during 14 h continuous measurement (>98% over 7 repetitive measurements with l-1a). The platform also enabled fully automatic multiparameter measurements with a single biocatalyst loading of about 1 mg PAL-MNP. Computational fluid dynamics (CFD) calculations were used to study the flow field in the chambers and the effect of unintended bubble formation. Optimal flow rate for l-1a reaction and specific activities for rac-1b–f under these conditions were determined.

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Cover Journal of Flow Chemistry
Journal of Flow Chemistry A Journal of the Flow Chemistry Society
Print ISSN:
2062-249X
Online ISSN:
2063-0212

To see the editorial board, please visit the website of Springer Nature.

Manuscript Submission: HERE

For subscription options, please visit the website of Springer Nature.

Journal of Flow Chemistry
Language English
Size A4
Year of
Foundation		 2011
Volumes
per Year		 1
Issues
per Year		 4
Founder Áramlásos Kémiai Tudományos Társaság
Founder's
Address		 H-1031 Budapest, Hungary Záhony utca 7.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 2062-249X (Print)
ISSN 2063-0212 (Online)

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