European Journal of Microbiology and Immunology
Volume/Issue:
Volume 2: Issue 3
Campylobacter jejuni induces extra-intestinal immune responses via toll-like-receptor-4 signaling in conventional IL-10 deficient mice with chronic colitis
Authors:
B. Otto Department of Microbiology and Hygiene, Charité — University Medicine Berlin, Berlin, Germany

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L.-M. Haag Department of Microbiology and Hygiene, Charité — University Medicine Berlin, Berlin, Germany

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A. Fischer Department of Microbiology and Hygiene, Charité — University Medicine Berlin, Berlin, Germany

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R. Plickert Department of Microbiology and Hygiene, Charité — University Medicine Berlin, Berlin, Germany

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A. A. Kühl Department of Internal Medicine, Rheumatology and Clinical Immunology / Research Center Immuno-Sciences (RCIS), Charité — University Medicine Berlin, Berlin, Germany

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U. B. Göbel Department of Microbiology and Hygiene, Charité — University Medicine Berlin, Berlin, Germany

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Markus M. Heimesaat Department of Microbiology and Hygiene, Charité — University Medicine Berlin, Berlin, Germany
Department of Microbiology and Hygiene, Campus Benjamin Franklin, Charité — University Medicine Berlin, CC5, Hindenburgdamm 27, D-12203, Berlin, Germany

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S. Bereswill Department of Microbiology and Hygiene, Charité — University Medicine Berlin, Berlin, Germany

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Pages:
210–219
Online Publication Date:
01 Sep 2012
Publication Date:
01 Sep 2012
Article Category:
Research Article
DOI:
https://doi.org/10.1556/eujmi.2.2012.3.7
Keywords:
Campylobacter jejuni ; chronic colitis; IL-10; TLR-2; TLR-4; lipopeptide; lipopolysaccharide; host-pathogen interaction; innate and adaptive immune responses; pro-inflammatory cytokines; conventional intestinal microbiota; GB, gnotobiotic; CR, colonization resistance; p.i., post infection; CFU, colony forming units; spp., species; IBD, inflammatory bowel diseases; TLR, Toll-like receptor; PAMP, pathogen-associated molecular patterns; LPS, lipopolysaccharide; LOS, lipooligosaccharide; LP, lipoprotein; MLN, mesenteric lymph nodes; DC, dendritic cell; Th1, T helper 1 cell; NaCl, natrium chloride; EDTA, ethylene diamine tetraacetic acid; FCS, fetal calf serum; NF-κB, nuclear factor ‘kappa-light-chain-enhancer’ of activated B-cells; MUC, mucin; HPRT, hypoxanthine phosphoribosyltransferase 1; PBS, phosphate buffered saline; TNF, tumor necrosis factor; IFN, interferon; MCP, monocyte chemotactic protein
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Abstract

Campylobacter jejuni is one of the predominant causes for foodborne bacterial infections worldwide. We investigated whether signaling of C. jejuni-lipoproteins and -lipooligosaccharide via Toll-like-receptor (TLR) -2 and -4, respectively, is inducing intestinal and extra-intestinal immune responses following infection of conventional IL-10-/- mice with chronic colitis. At day 3 following oral infection, IL-10-/- mice lacking TLR-2 or TLR-4 harbored comparable C. jejuni strain ATCC 43431 loads in their colon. Interestingly, infected TLR-4-/- IL-10-/- mice displayed less compromized epithelial barrier function as indicated by lower translocation rates of live gut commensals into mesenteric lymphnodes (MLNs), and exhibited less distinct B lymphocyte responses in their colonic mucosa as compared to naïve IL-10-/- controls. Furthermore, in extra-intestinal compartments such as MLNs and spleens, abundance of myeloid cells was less distinct whereas relative percentages of activated T helper cells and cytotoxic T cells were higher in spleens and dendritic cells more abundant in MLNs of infected IL-10-/- animals lacking TLR-4 as compared to IL-10-/- controls. Taken together, in conventionally colonized IL-10-/- mice, TLR-4, but not TLR-2, is involved in mediating extra-intestinal pro-inflammatory immune responses following C. jejuni infection. Thus, conventional IL-10-/- mice are well suited to further dissect mechanisms underlying Campylobacter infections in vivo.

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Cover European Journal of Microbiology and Immunology
European Journal of Microbiology and Immunology
Print ISSN:
2062-509X
Online ISSN:
2062-8633

Senior editors

Editor(s)-in-Chief: Dunay, Ildiko Rita, Prof. Dr. Pharm, Dr. rer. nat., University of Magdeburg, Germany

Editor(s)-in-Chief: Heimesaat, Markus M., Prof. Dr. med., Charité - University Medicine Berlin, Germany

Editorial Board

  • Berit Bangoura, Dr. DVM. PhD,  University of Wyoming, USA
  • Stefan Bereswill, Prof. Dr. rer. nat., Charité - University Medicine Berlin, Germany
  • Dunja Bruder, Prof. Dr. rer. nat., University of Magdeburg, Germany
  • Jan Buer, Prof. Dr. med., University of Duisburg, Germany
  • Edit Buzas, Prof. Dr. med., Semmelweis University, Hungary
  • Renato Damatta, Prof. PhD, UENF, Brazil
  • Maria Deli, MD, PhD, DSc, Biological Research Center, HAS, Hungary
  • Olgica Djurković-Djaković, Prof. Phd, University of Belgrade, Serbia
  • Jean-Dennis Docquier, Prof. Dr. med., University of Siena, Italy
  • Zsuzsanna Fabry, Prof. Phd, University of Washington, USA
  • Ralf Ignatius, Prof. Dr. med., Charité - University Medicine Berlin, Germany
  • Achim Kaasch, Prof. Dr. med., Otto von Guericke University Magdeburg, Germany
  • Oliver Liesenfeld, Prof. Dr. med., Inflammatix, USA
  • Matyas Sandor, Prof. PhD, University of Wisconsin, USA
  • Ulrich Steinhoff, Prof. PhD, University of Marburg, Germany
  • Michal Toborek, Prof. PhD, University of Miami, USA
  • Susanne A. Wolf, PhD, MDC-Berlin, Germany

 

Dr. Dunay, Ildiko Rita
Magdeburg, Germany
E-mail: ildiko.dunay@med.ovgu.de

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European Journal of Microbiology and Immunology
Language English
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Foundation		 2011
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per Year		 1
Issues
per Year		 4
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ISSN 2062-509X (Print)
ISSN 2062-8633 (Online)

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