Authors:
, , , , , , and
Authors:
,
, and
Desiree J. Meyer
Desiree J. Meyer
School of Medicine and Pharmacology, University of Western Australia, Perth, Australia
Search for other papers by Desiree J. Meyer in
Current site
Google Scholar
PubMed
Search for other papers by Desiree J. Meyer in
Current site
Google Scholar
PubMed
Jane E. Allan
Jane E. Allan
School of Medicine and Pharmacology, University of Western Australia, Perth, Australia
Search for other papers by Jane E. Allan in
Current site
Google Scholar
PubMed
Search for other papers by Jane E. Allan in
Current site
Google Scholar
PubMed
Miles H. Beaman
Miles H. Beaman
School of Biomedical, Biomolecular and Chemical Sciences, University of Western Australia, Perth, Australia
Department of Medicine, University of Notre Dame Australia, Fremantle Campus, Western Australia, Fremantle, Australia
Western Diagnostic Pathology, 74 McCoy St. Myaree, Western Australia, Fremantle, 6154, Australia
Search for other papers by Miles H. Beaman in
Current site
Google Scholar
PubMed
Department of Medicine, University of Notre Dame Australia, Fremantle Campus, Western Australia, Fremantle, Australia
Western Diagnostic Pathology, 74 McCoy St. Myaree, Western Australia, Fremantle, 6154, Australia
Search for other papers by Miles H. Beaman in
Current site
Google Scholar
PubMed
Abstract
Toxoplasma gondii (T. gondii) causes serious infection, especially in immunocompromised hosts. The relevance of animal models of toxoplasmosis to human disease is unclear, but have indicated that the route of Toxoplasma infection may affect the outcome. A humanized model of toxoplasmosis of immunocompromised mice (i.e. hu-PBL SCID), using the intraperitoneal (IP) route demonstrated long-term engraftment of human cells and worsening of inflammation compared to controls. In this study, we examined the effect of route of infection on this hu-PBL SCID model using a Toxoplasma strain (i.e. DAG) isolated from an immunocompromised human. Oral infection led to an asymptomatic infection, whereas animals infected by the IP route succumbed more quickly to infection. Human cells, detected through species-specific β-actin mRNA, were not as prominent in IP-infected animals as compared to orally infected and uninfected animals. There was evidence of control of toxoplasmosis in some orally infected animals, and this was associated with the presence of human cells in multiple tissues. Thus, the route of infection dramatically affects the outcome of infection, either by affecting parasite replication or expansion of human immune cells. Further studies of oral Toxoplasma infection using hu-PBL SCID mice may help in developing chemotherapies and immunotherapeutic strategies for toxoplasmosis.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1. J.K. Frenkel 1988 Pathophysiology of toxoplasmosis Parasitol Today 4 273 278.
-
2. M. Beaman 2010 The role of immunomodulation in the man agement of infections of the CNS T. Kilpatrick S. Wesslingh R. Ransonohoff In Inflammatory Diseases of the Central Nervous Syste. Cambridge University Press Cam bridge 221 244.
-
3. A.M. Johnson 1984 Strain-dependent, route of challenge-dependent, murine susceptibility to toxoplasmosis Z Parasitenkd 70 303 309.
-
4. R. McLeod R.G. Estes D.G. Mack H. Cohen 1984 Immune response of mice to ingested Toxoplasma gondii: a model of toxoplasma infection acquired by ingestion J Infect Dis 149 234 244.
-
5. O. Liesenfeld J. Kosek J.S. Remington Y. Suzuki 1996 Association Of Cd4(+) T cell-dependent, interferon-gamma-mediated necrosis of the small intestine with genetic susceptibility of mice to peroral infection with Toxoplasma gondii J Exper Med 184 597 607.
-
6. M.H. Beaman J.S. Remington D.J. Meyer 2000 Human peripheral blood lymphocyte severe combined immunodeficiency (hu-PBL SCID) models of toxoplasmosis Immunol Cell Biol 78 608 615.
-
7. D.J. Meyer J.E. Allan M.H. Beaman 2000 Distribution of parasite stages in tissues of Toxoplasma gondii infected SCID mice and human peripheral blood lymphocyte-transplanted SCID mice Parasite Immunol 22 567 579.
-
8. Y. Suzuki F.K. Conley J.S. Remington 1989 Differences in virulence and development of encephalitis during chronic infection vary with the strain of Toxoplasma gondii J Infect Dis 159 790 794.
-
9. D.A. Blewett J.K. Miller J. Harding 1983 Simple technique for the direct isolation of toxoplasma tissue cysts from fetal ovine brain Vet Rec 112 98 100.
-
10. J. Huskinson-Mark F.G. Araujo J.S. Remington 1991 Evaluation of the effect of drugs on the cyst form of Toxoplasma gondii J Infect Dis 164 170 177.
-
11. G. Desmonts J.S. Remington 1980 Direct agglutination test for diagnosis of Toxoplasma infection: method for increasing sensitivity and specificity J Clin Microbiol 11 562 568.
-
12. W. Bohne U. Gross D.J. Ferguson J. Heesemann 1995 Cloning and characterisation of a bradyzoite-specifically expressed gene (hsp30/bag1) of Toxoplasma gondii, related to genes encoding small heat shock proteins of plants Mol Microbiol 16 1221 1230.
-
13. L. Burg D. Perelman L.H. Kasper P.L. Ware J.C. Boothroyd 1988 Molecular analysis of the gene encoding the major surface antigen of Toxoplasma gondii J Immunol 141 3584 3591.
-
14. R.T. Gazzinelli I. Eltoum T.A. Wynn A. Sher 1993 Acute cerebral toxoplasmosis is induced by in vivo neutralization of tnf-alpha and correlates with the down-regulated expression of inducible nitric oxide synthase and other markers of macrophage activation J Immunol 151 3672 3681.
-
15. J.C. Boothroyd J. Burg S. Nagel D. Perelman L. Kasper P. Ware J. Prince S. Sharma J. Remington 1987 Antigen and tubulin genes of Toxoplasma gondii N. Agabian H. Goodman N. Nogueira R. Alan Molecular Strategies of Parasitic Invasion Liss Inc. New York 237 250.
-
16. J. Burg C.M. Grover P. Pouletty J.C. Boothroyd 1989 Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction J Clin Microbiol 27 1787 1792.
-
17. I. Autenrieth N. Bucheler E. Bohn G. Heinze I. Horak 1997 Cytokine mRNA expression in intestinal tissue of inter leukin-2 deficient mice with bowel inflammation Gut 41 793 800.
-
18. Sambrook J , Fritsch E, Maniatis T (1989): Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press.
-
19. B. Dawson-Saunders R.G. Trapp 1990 Basic and Clinical Statistics Appleton and Lange San Mateo, CA.
-
20. L. Schultz M.A. Brehm J.V. Garcia-Martinez D.L. Greiner 2012 Humanized mice for immune system investigation: progress, promise and challenges Nature Rev Immunol 12 786 798.
-
21. N. Courret S. Darche P. Sonigo G. Milon D. Buzoni-Gatel I. Tardieux 2006 CD 11c and CD11b-expressing mouse leukocytes transport single Toxoplasma gondii tachyzoites to the brain Blood 107 309 316.
-
22. B. Butcher L. Kim A.D. Panapoulos S.S. Watowich P.J. Murray E.Y. Denkers 2005 Cutting edge: IL-10-independent STAT3 activation by Toxoplasma gondii mediates suppression of IL-12 and TNF-a in host macrophages J Immunol 174 3148 3152.
Please, download the file from HERE.
Dr. Dunay, Ildiko Rita
Magdeburg, Germany
E-mail: ildikodunay@gmail.com
Indexing and Abstracting Services:
- PubMed Central
- Scopus
- ESCI
- CABI
- CABELLS Journalytics
| 2022 | |
|---|---|
| Web of Science | |
| Total Cites WoS |
717 |
| Journal Impact Factor | 2.2 |
| Rank by Impact Factor |
n/a |
| Impact Factor without Journal Self Cites |
2.2 |
| 5 Year Impact Factor |
2.8 |
| Journal Citation Indicator | 0.66 |
| Rank by Journal Citation Indicator |
Microbiology (Q2) |
| Scimago | |
| Scimago H-index |
11 |
| Scimago Journal Rank |
0.614 |
| Scimago Quartile Score | Microbiology (Q3) Microbiology (medical) (Q3) Immunology and Allergy (Q3) Immunology (Q3) |
| Scopus | |
| Scopus Cite Score |
8.3 |
| Scopus CIte Score Rank |
Microbiology 33/163 (80th PCTL) Microbiology (medical) 28/124 (77th PCTL) Immunology and Allergy 63/211 (70th PCTL) Immunology 69/217 (68th PCTL) |
| Scopus SNIP |
1.221 |
| 2021 | |
|---|---|
| Web of Science | |
| Total Cites WoS |
790 |
| Journal Impact Factor | not applicable |
| Rank by Impact Factor | not applicable |
| Impact Factor without Journal Self Cites |
not applicable |
| 5 Year Impact Factor |
not applicable |
| Journal Citation Indicator | 0,64 |
| Rank by Journal Citation Indicator | Microbiology 81/157 |
| Scimago | |
| Scimago H-index |
not indexed |
| Scimago Journal Rank |
not indexed |
| Scimago Quartile Score | not indexed |
| Scopus | |
| Scopus Cite Score |
not indexed |
| Scopus CIte Score Rank |
not indexed |
| Scopus SNIP |
not indexed |
| 2020 | |
|---|---|
| CrossRef Documents | 23 |
| WoS Cites | 708 |
| Wos H-index | 27 |
| Days from submission to acceptance | 219 |
| Days from acceptance to publication | 176 |
| Acceptance Rate | 70% |
| 2019 | |
|---|---|
| WoS Cites |
558 |
| CrossRef Documents |
24 |
| Acceptance Rate |
92% |
| European Journal of Microbiology and Immunology | |
|---|---|
| Publication Model | Gold Open Access |
| Submission Fee | none |
| Article Processing Charge | 600 EUR/article |
| Regional discounts on country of the funding agency | World Bank Lower-middle-income economies: 50% World Bank Low-income economies: 100% |
| Further Discounts | Editorial Board / Advisory Board members: 50% Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100% |
| Subscription Information | Gold Open Access |
| Purchase per Title | |
| European Journal of Microbiology and Immunology | |
|---|---|
| Language | English |
| Size | A4 |
| Year of Foundation |
2011 |
| Volumes per Year |
1 |
| Issues per Year |
4 |
| Founder | Akadémiai Kiadó |
| Founder's Address |
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. |
| Publisher | Akadémiai Kiadó |
| Publisher's Address |
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. |
| Responsible Publisher |
Chief Executive Officer, Akadémiai Kiadó |
| ISSN | 2062-509X (Print) |
| ISSN | 2062-8633 (Online) |
Monthly Content Usage
| Abstract Views | Full Text Views | PDF Downloads | |
|---|---|---|---|
| Jun 2023 | 7 | 0 | 0 |
| Jul 2023 | 3 | 0 | 0 |
| Aug 2023 | 7 | 0 | 0 |
| Sep 2023 | 2 | 0 | 0 |
| Oct 2023 | 6 | 1 | 0 |
| Nov 2023 | 4 | 2 | 0 |
| Dec 2023 | 0 | 0 | 0 |
Author:
Copyright Akadémiai Kiadó AKJournals is the trademark of Akadémiai Kiadó's journal publishing business branch.
Powered by PubFactory