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  • 1 Departamento de Biologia and CESAM, Universidade de Aveiro, 3810-193, Aveiro, Portugal
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The aim of this study was to make a comparison between the results obtained by cytologies and by the detection and genotyping of human papillomavirus (HPV) DNA in the screening of cervical cancer. In this study, there were 994 samples used from human females. These were obtained from liquid-based preparations. The samples were analyzed by cytological technique and by the detection of HPV DNA using the PapilloCheck® Test. The HPV was detected in 28% of the samples. Most of the cytology lesions appeared in HPV positive samples and, within these, the most serious injuries occurred mostly in samples with multiple HPV infections. The results indicate that, in general, there is a correlation between the detection of HPV DNA and cytology. However, there were some cases that emphasize the limitations of both diagnosis methods (27% cases with viral HPV DNA positive and normal cytologies and about 2% of cytological lesions detected in samples HPV negatives). It is possible to conclude that none of the two techniques is enough by itself and should be applied together in order to increase the accuracy of cervical cancer screening.

  • 1. S. Shukla et al.2009 Infection of human papillomaviruses in cancers of different human organ sites Indian J Med Res 1303 222 233.

  • 2. J.T.T. Nakagawal et al.2010 Vírus HPV e câncer de colo de útero Rev Bras Enferm 63 2 307 311.

  • 3. J. Su et al.2010 Immunotherapy for cervical cancer: research status and clinical potential Biodrugs 24 2 109 129.

  • 4. S. Leon et al.2011 Distribution patterns of infection with multiple types of human papillomaviruses and their association with risk factors Publ Libr Sci — PloS ONE. 6 2 1 7.

    • Search Google Scholar
    • Export Citation
  • 5. Ventura MT et al. (2008): Vacinação contra infeções por Vírus do Papiloma Humano (HPV). Direcção-Geral da Saúde.

  • 6. L. Bruni et al.2010 Cervical human papillomavirus prevalence in 5 continents: meta-analysis of 1 million women with normal cytological findings J Infect Dis 202 12 1789 1799.

    • Search Google Scholar
    • Export Citation
  • 7. A. Pista et al.2011 Prevalence of human papillomavirus infection in women in Portugal — the CLEOPATRE Portugal study Int J Gynecol Cancer 21 6 1150 1158.

    • Search Google Scholar
    • Export Citation
  • 8. B.S. Apgar et al.2003 The 2001 Bethesda System Terminology Am Fam Phys 68 10 1993 1998.

  • 9. Z. Guglielmo A. Rodriguez 2010 Métodos utilizados en la identificacióndelvirus de papiloma humano An del Sist Sanit Navar 33 1 71 77.

    • Search Google Scholar
    • Export Citation
  • 10. M.P. Cañadas et al.2006 Evaluación de las técnicas de deteccióndel VPH en los programas de cribado para cáncer de cuellzo uterino Salud Públ Méx 48 5 373 378.

    • Search Google Scholar
    • Export Citation
  • 11. S. Tsiodras et al.2010 Hybrid capture vs. PCR screening of cervical human papilloma virus infections. Cytological and histological associations in 1270 women Biomed Cent Cancer 10 53 1 8.

    • Search Google Scholar
    • Export Citation
  • 12. V. Dalsteina et al.2009 Analytical evaluation of the PapilloCheck test, a new commercial DNA chip for detection and genotyping of human papillomavirus J Virol Method 156 77 83.

    • Search Google Scholar
    • Export Citation
  • 13. Pacheco A et al. (2011): Consenso sobre infecção HPV e lesões intraepiteliais do colo, vagina e vulva. Sociedade Portuguesa de Ginecologia; Secção Portuguesa de Colposcopia e Patologia Cervico-Vulvovaginal.

    • Search Google Scholar
    • Export Citation
  • 14. M. Poljak B.J. Kocjan 2010 Commercially available assays for multiplex detection of alpha human papillomaviruses Expert Rev Anti-Infect Ther 8 10 1139 1162.

    • Search Google Scholar
    • Export Citation
  • 15. P.J.F. Snijders et al.2010 Methods for HPV detection in exfoliated cell and tissue specimens Acta Pathol Microbiol Immunol Scand 118 6–7 520 528.

    • Search Google Scholar
    • Export Citation
  • 16. J. Cuzick et al.1999 HPV testing in primary screening of older women Br J Cancer 81 3 554 558.

  • 17. A.T. Hesselink et al.2010 Comparison of the clinical performance of PapilloCheck human papillomavirus detection with that of the GP5+/6+-PCR enzyme immunoassay in populationbased cervical screening J Clin Microbiol 483 797 801.

    • Search Google Scholar
    • Export Citation
  • 18. Greiner Bio-One GmbH (2009): PapilloCheck® Manual. Germany.

  • 19. Decreto LeiN° 190 — 17 de Agosto de 2001. Diário da República n° 129/2001-I Série-B. Ministério da Saúde, Lisboa.

  • 20. Hologic, Inc. 2000 The Thin-Prep Pap Test Hologic, Inc New York, USA.

  • 21. Greiner Bio-One GmbH (2009): PapilloCheck® DNA Extraction kit — Instructions for Use, Germany.

  • 22. Rosenblatt C (2011): HPV (Papilomavírus humano). Brasil: hpvinfo

  • 23. S. Sanjose et al.2007 Worldwide prevalence and genotype distribution of cervical human papillomavirus DNA in women with normal cytology: a meta-analysis Lancet Infect Dis 7 453 459.

    • Search Google Scholar
    • Export Citation
  • 24. N. Muñoz et al.2003 Epidemiologic classification of human papillomavirus types associated with cervical cancer N Engl J Med 3486 518 527.

    • Search Google Scholar
    • Export Citation
  • 25. G.M. Clliford IARC HPV Prevalence Surveys Study Group et al.2005 Worldwide distribution of human papillomavirus types in cytologically normal women in the International Agency for Research on Cancer HPV prevalence surveys: a pooled analysis Lancet 366 991 998.

    • Search Google Scholar
    • Export Citation
  • 26. R.J. Nobre et al.2010 Characterization of common and rare human papillomaviruses in Portuguese women by the polymerase chain reaction, restriction fragment length polymorphism and sequencing J Med Virol 826 1024 1032.

    • Search Google Scholar
    • Export Citation
  • 27. E. Murta et al.2000 Incidence of Gardnerella vaginalis, Candida sp. and human papilloma virus in cytological smears São Paulo Med J 118 4 105 108.

    • Search Google Scholar
    • Export Citation
  • 28. E. Samoff et al.2005 Association of Chlamydia trachomatis with persistence of high-risk types of human papillomavirus in a cohort of female adolescents Am J Epidemiol 16279 668 675.

    • Search Google Scholar
    • Export Citation
  • 29. C.Y. Lee W. Ng 2007 A follow-up study of atypical squamous cells in gynecologic cytology using conventional papanicolaou smears and liquid-based preparations Am J Clin Pathol 127 548 555.

    • Search Google Scholar
    • Export Citation
  • 30. A. Nascimento E.S. Cibas 2007 The ASC/SIL ratio for cytopathologists as a quality control measure Am J Clin Pathol 128 653 656.

  • 31. Gouveia S (2009): Detecção de DNA de HPV oncogénico por captura híbrida em citologias normais. Dissertation, Universidade de Aveiro.

    • Search Google Scholar
    • Export Citation
  • 32. H. Trottier et al.2006 Human papillomavirus infections with multiple types and risk of cervical neoplasia Cancer Epidemiol Biomark Prev 15 7 1274 1280.

    • Search Google Scholar
    • Export Citation
  • 33. Pereira AST (2010): Genotipagem do vírus do Papiloma Humano em citologia cérvico-vaginal. Dissertation, Universidade de Aveiro.

  • 34. A. Pista et al.2011 Single and multiple human papillomavirus infections in cervical abnormalities in Portuguese women Clin Microbiol Infect 17 941 946.

    • Search Google Scholar
    • Export Citation
  • 35. Cavaco A et al. (2010): Vacinas contra o HPV — Reunião de Consenso Nacional. Sociedade Portuguesa de Ginecologia, 127.

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Senior editors

Editor(s)-in-Chief: Dunay, Ildiko Rita

Editor(s)-in-Chief: Heimesaat, Markus M.

Vice Editor(s)-in-Chief: Fuchs, Anja

Editorial Board

Chair of the Editorial Board:
Jeffrey S. Buguliskis (Thomas Jefferson University, USA)

  • Jörn Albring (University of Münster, Germany)
  • Stefan Bereswill (Charité - University Medicine Berlin, Germany)
  • Dunja Bruder (University of Megdeburg, Germany)
  • Jan Buer (University of Duisburg, Germany)
  • Jeff Buguliskis (Thomas Jefferson University, USA)
  • Edit Buzas (Semmelweis University, Hungary)
  • Charles Collyer (University of Sydney, Australia)
  • Renato Damatta (UENF, Brazil)
  • Ivelina Damjanova (Semmelweis University, Hungary)
  • Maria Deli (Biological Research Center, HAS, Hungary)
  • Olgica Djurković-Djaković (University of Belgrade, Serbia)
  • Jean-Dennis Docquier (University of Siena, Italy)
  • Anna Erdei (Eötvös Loránd University, Hungary)
  • Zsuzsanna Fabry (University of Washington, USA)
  • Beniam Ghebremedhin (Witten/Herdecke University, Germany)
  • Nancy Guillen (Institute Pasteur, France)
  • Georgina L. Hold (University of Aberdeen, United Kingdom)
  • Ralf Ignatius (Charité - University Medicine Berlin, Germany)
  • Zsuzsanna Izsvak (MDC-Berlin, Germany)
  • Achim Kaasch (University of Cologne, Germany)
  • Tamás Laskay (University of Lübeck, Germany)
  • Oliver Liesenfeld (Roche, USA)
  • Shreemanta Parida (Vaccine Grand Challenge Program, India)
  • Matyas Sandor (University of Wisconsin, USA)
  • Ulrich Steinhoff (University of Marburg, Germany)
  • Michal Toborek (University of Miami, USA)
  • Mary Jo Wick (University of Gothenburg, Sweden)
  • Susanne A. Wolf (MDC-Berlin, Germany)


Dr. Dunay, Ildiko Rita
Magdeburg, Germany

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CrossRef Documents 23
WoS Cites 708
Wos H-index 27
Days from submission to acceptance 219
Days from acceptance to publication 176
Acceptance Rate 70%



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European Journal of Microbiology and Immunology
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