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  • 1 Department of Medical Microbiology and Parasitology, P.M.B. 4400, Ladoke Akintola University of Technology, College of Health Sciences, Osogbo, Osun State, Nigeria
  • 2 Department of Bacteriology and Food Safety, Animal Health and Veterinary Laboratories Agency, Woodham Lane, New Haw, Surrey, KT15 3NB, UK
  • 3 Institut für Mikrobiologie und Tierseuchen, Freie Universität Berlin, Philippstrasse 13, 10115, Berlin, Germany
  • 4 Department of Medical Microbiology and Parasitology, University of Ibadan, Ibadan, Nigeria
  • 5 Department of Microbiology, Faculty of Sciences, University of Ilorin, Kwara State, Nigeria
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Abstract

Background

Escherichia coli have become the enterobacteriaceae species most affected by extended-spectrum β-lactamases (ESBLs) in view of the emergence of CTX-M-type ESBLs. These CTX-M-positive E. coli have been reported in numerous regions worldwide. Virulence determinants of already reported CTX-M-positive E. coli were investigated.

Methodology

To gain insights into the mechanism underlying this phenomenon, we assessed serogroup, susceptibility pattern and diversity of virulence profiles within a collection of nine blaCTX-M-positive E. coli strains and their virulent determinant using miniaturized DNA microarray techniques. The nine ESBL-positive E. coli isolates were from eight male and one female patient(s) selected for study based on previous work. Virulence potential was inferred by detection of 63 virulence factor (VF) genes.

Results

Four (44.4%) of the 9 E. coli isolates exhibited the same set of core characteristics: serotype O8:Hnt, while all were positive for OXA-1, ciprofloxacin resistance. Five of the isolates exhibited highly similar (91% to 100%) VF profiles.

Conclusion

The findings describe a broadly disseminated, blaCTX-M-positive and virulent E. coli serogroup with highly homogeneous virulence genotypes, suggesting recent emergence in this zone. Understanding how this clone has emerged and successfully disseminated within the hospital and community, including across national boundaries, should be a public health priority.

  • 1. P.S. Mead L. Slutsker V. Dietz 1999 Food-related illness and death in the United States Emerg Infect Dis 5 607 625.

  • 2. J.B. Kaper J.P. Nataro H.L. Mobley 2004 Pathogenic Escherichia coli Nat Rev Microbiol 2 123 140.

  • 3. J.P. Nataro J.B. Kaper 1998 Diarrheagenic Escherichia coli Clin Microbiol Rev 11 142 201.

  • 4. C.G. Clark S.T. Johnson R.H. Easy J.L. Campbell F.G. Rodgers 2002 PCR for detection of cdt-III and the relative frequencies of cytolethal distending toxin variant-producing Escherichia coli isolates from humans and cattle J Clin Microbiol 40 2671 2674.

    • Search Google Scholar
    • Export Citation
  • 5. J. Osek 2003 Detection of the enteroaggregative Escherichia coli heat-stable enterotoxin 1 (EAST1) gene and its relationship with fimbrial and enterotoxin markers in E. coli isolates from pigs with diarrhoea Vet Microbiol 91 65 72.

    • Search Google Scholar
    • Export Citation
  • 6. V.K. Sharma 2002 Detection and quantitation of enterohemorrhagic Escherichia coli O157, O111, and O26 in beef and bovine feces by real-time polymerase chain reaction J Food Prot 65 1371 1380.

    • Search Google Scholar
    • Export Citation
  • 7. G. Wang C.G. Clark F.G. Rodgers 2002 Detection in Escherichia coli of the genes encoding the major virulence factors, the genes defining the O157:H7 serotype, and components of the type 2 Shiga toxin family by multiplex PCR J Clin Microbiol 40 3613 3619.

    • Search Google Scholar
    • Export Citation
  • 8. D. Paterson R. Bonomo 2005 Extended-spectrum β-lactamases: a clinical update Clin Microbiol Rev 18 657 686.

  • 9. J.D. Pitout P. Nordmann K.B. Laupland 2005 Emergence of Enterobacteriaceae producing extended-spectrum b-lactamases (ESBLs) in the community J Antimicrob Chemother 56 52 59.

    • Search Google Scholar
    • Export Citation
  • 10. R. Canton T.M. Coque 2006 The CTX-M b-lactamase pandemic Curr Opin Microbiol 9 466 475.

  • 11. R. Bonnet 2004 Growing group of extended-spectrum b-lactamases: the CTX-M enzymes Antimicrob Agents Chemother 48 1 14.

  • 12. D.A. Boyd S. Tyler S. Christianson 2004 Complete nucleotide sequence of a 92-kilobase plasmid harboring the CTXM-15 extended-spectrum β-lactamase involved in an outbreak in long-term-care facilities in Toronto, Canada Antimicrob Agents Chemother 48 3758 3764.

    • Search Google Scholar
    • Export Citation
  • 13. E. Karisik M.J. Ellington R. Pike R.E. Warren D.M. Livermore N. Woodford 2006 Molecular characterization of plasmids encoding CTX-M-15 ß-lactamase from Escherichia coli strains in the United Kingdom J Antimicrob Chemother 58 665 668.

    • Search Google Scholar
    • Export Citation
  • 14. M. Lavollay K. Mamlouk T. Frank 2006 Clonal dissemination of a CTX-M-15 β-lactamase-producing Escherichia coli strain in the Paris area, Tunis, and Bangui Antimicrob Agents Chemother 50 2433 2438.

    • Search Google Scholar
    • Export Citation
  • 15. A. Olowe G. Mirjam B. Britta L.B. Antina A. Fruth L.H. Wieler 2010 Detection of blaCTX-M-15 extended-spectrum β-lactamase genes in Escherichia coli from hospital patients in Nigeria Int J Antimicrob Agents 3 206 207.

    • Search Google Scholar
    • Export Citation
  • 16. National Committee for Clinical Laboratory Standards Performance standards for antimicrobial diskdilution susceptibility tests for bacteria isolated from animals. 2002 Approved standard M31-A2 2nd ed National Committee for Clinical Laboratory Standards Wayne, PA.

    • Search Google Scholar
    • Export Citation
  • 17. P.A.M. Guinée W.H. Jansen T. Wadström 1981 Escherichia coli associated with neonatal diarrhoea in piglets and calves P.W. Leeww P.A.M. Guinée Laboratory Diagnosis in Neonatal Calf and Pig Diarrhoea: Current Topics in Veterinary and Animal Science Martinus-Nijhoff The Hague, The Netherlands 126 62.

    • Search Google Scholar
    • Export Citation
  • 18. Bert F , Branger C, Lambert-Zechovsky N: Identification of PSE and OXA ta-lactamase genes in Pseudomonas aeruginosa using PCR-restriction fragment length polymorphism. J Antimicrob Chemother 50, 11–18 (2002).

    • Search Google Scholar
    • Export Citation
  • 19. M.F. Anjum M. Mafura P. Slickers 2007 Pathotyping Escherichia coli by using miniaturized DNA microarrays Appl Environ Microbiol 73 5692 5697.

    • Search Google Scholar
    • Export Citation
  • 20. K. Ballmer B.M. Korczak P. Kuhnert P. Slickers R. Ehricht H. Hachler 2007 Fast DNA serotyping of Escherichia coli by use of an oligonucleotide microarray J Clin Microbiol 45 370 379.

    • Search Google Scholar
    • Export Citation
  • 21. S. Monecke R. Ehricht 2005 Rapid genotyping of methicillin-resistant Staphylococcus aureus (MRSA) isolates using miniaturised oligonucleotide arrays Clin Microbiol Infect 11 825 833.

    • Search Google Scholar
    • Export Citation
  • 22. G. Bruant C. Maynard S. Bekal I. Gaucher L. Masson R. Brousseau J. Harel 2006 Development and validation of an oligonucleotide microarray for detection of multiple virulence and antimicrobial resistance genes in Escherichia coli Appl Environ Microbiol 72 3780 3784.

    • Search Google Scholar
    • Export Citation
  • 23. D.R. Call F.J. Brockman D.P. Chandler 2001 Detecting and genotyping Escherichia coli O157:H7 using multiplexed PCR and nucleic acid microarrays Int J Food Microbiol 67 71 80.

    • Search Google Scholar
    • Export Citation
  • 24. S. Chen S. Zhao P.F. McDermott C.M. Schroeder D.G. White J. A. Meng 2005 DNA microarray for identification of virulence and antimicrobial resistance genes in Salmonella serovars and Escherichia coli Mol Cell Probes 19 195 201.

    • Search Google Scholar
    • Export Citation
  • 25. V. Chizhikov A. Rasooly K. Chumakov D.D. Levy 2001 Microarray analysis of microbial virulence factors Appl Environ Microbiol 67 3258 3263.

    • Search Google Scholar
    • Export Citation
  • 26. C. Jenkins C. van Ijperen E.G. Dudley H. Chart G.A. Willshaw T. Cheasty H.R. Smith J.P. Nataro 2005 Use of a microarray to assess the distribution of plasmid and chromosomal virulence genes in strains of enteroaggregative Escherichia coli FEMS Microbiol Lett 253 119 124.

    • Search Google Scholar
    • Export Citation
  • 27. B. Korczak J. Frey J. Schrenzel 2005 Use of diagnostic microarrays for determination of virulence gene patterns of Escherichia coli K1, a major cause of neonatal meningitis J Clin Microbiol 43 1024 1031.

    • Search Google Scholar
    • Export Citation
  • 28. C.O. Branger S. Zamfir G. Geoffroy 2005 Genetic background of Escherichia coli and extended-spectrum β-lactamase type Emerg Infect Dis 11 54 61.

    • Search Google Scholar
    • Export Citation

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