Authors:
Miha Skvarc Faculty of Medicine, Institute of Microbiology and Immunology, Ljubljana, Slovenia

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David Stubljar Faculty of Medicine, Institute of Microbiology and Immunology, Ljubljana, Slovenia

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Petra Rogina General Hospital, Novo Mesto, Slovenia

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Achim J. Kaasch Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, 50935, Cologne, Germany
Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Goldenfelsstr. 19-21, 50935, Köln, Germany

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Abstract

Bloodstream infections are a major cause of morbidity and mortality worldwide. Molecular methods for the detection of pathogens in blood have been developed. The clinical utility of these methods and their integration into the clinical workflow is discussed.

  • 1. R.C. Bone R.A. Balk F.B. Cerra R.P. Dellinger A.M. Fein W.A. Knaus R.M. Schein W.J. Sibbald 1992 Definitions for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis. The ACCP/SCCM Consensus Conference Committee. American College of Chest Physicians/Society of Critical Care Medicine Chest 101 1644 1655.

    • Search Google Scholar
    • Export Citation
  • 2. M.M. Levy A. Artigas G.S. Phillips A. Rhodes R. Beale T. Osborn J.L. Vincent S. Townsend S. Lemeshow R.P. Dellinger 2012 Outcomes of the Surviving Sepsis Campaign in intensive care units in the USA and Europe: a prospective cohort study Lancet Infect Dis 12 919 924.

    • Search Google Scholar
    • Export Citation
  • 3. A. Kumar D. Roberts K.E. Wood B. Light J.E. Parrillo S. Sharma R. Suppes D. Feinstein S. Zanotti L. Taiberg D. Gurka M. Cheang 2006 Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock Crit Care Med 34 1589 1596.

    • Search Google Scholar
    • Export Citation
  • 4. R.P. Dellinger M.M. Levy A. Rhodes D. Annane H. Gerlach S.M. Opal J.E. Sevransky C.L. Sprung I.S. Douglas R. Jaeschke T.M. Osborn M.E. Nunnally S.R. Townsend K. Reinhart R.M. Kleinpell D.C. Angus C.S. Deutschman F.R. Machado G.D. Rubenfeld S.A. Webb R.J. Beale J.L. Vincent R. Moreno 2013 Surviving sepsis campaign: international guidelines for management of severe sepsis and septic shock: 2012 Crit Care Med 41 580 637.

    • Search Google Scholar
    • Export Citation
  • 5. M. Paolucci M.P. Landini V. Sambri 2010 Conventional and molecular techniques for the early diagnosis of bacteraemia Int J Antimicrob Agents 36 Suppl2 S6 16.

    • Search Google Scholar
    • Export Citation
  • 6. M.L. Lorente M. Falguera A. Nogues A.R. Gonzalez M.T. Merino M.R. Caballero 2000 Diagnosis of pneumococcal pneumonia by polymerase chain reaction (PCR) in whole blood: a prospective clinical study Thorax 55 133 137.

    • Search Google Scholar
    • Export Citation
  • 7. J. Wheeler O.M. Murphy R. Freeman A.M. Kearns M. Steward M.J. Lee 2000 PCR can add to detection of pneumococcal disease in pneumonic patients receiving antibiotics at admission J Clin Microbiol 38 3907.

    • Search Google Scholar
    • Export Citation
  • 8. T. Avni N. Mansur L. Leibovici M. Paul 2010 PCR using blood for diagnosis of invasive pneumococcal disease: systematic review and meta-analysis J Clin Microbiol 48 489 496.

    • Search Google Scholar
    • Export Citation
  • 9. R.P. Peters M.A. van Agtmael S.A. Danner P.H. Savelkoul C.M. Vandenbroucke-Grauls 2004 New developments in the diagnosis of bloodstream infections Lancet Infect Dis 4 751 760.

    • Search Google Scholar
    • Export Citation
  • 10. L.E. Lehmann K.P. Hunfeld T. Emrich G. Haberhausen H. Wissing A. Hoeft F. Stuber 2008 A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples Med Microbiol Immunol 197 313 324.

    • Search Google Scholar
    • Export Citation
  • 11. H. Westh G. Lisby F. Breysse B. Boddinghaus M. Chomarat V. Gant A. Goglio A. Raglio H. Schuster F. Stuber H. Wissing A. Hoeft 2009 Multiplex real-time PCR and blood culture for identification of bloodstream pathogens in patients with suspected sepsis Clin Microbiol Infect 15 544 551.

    • Search Google Scholar
    • Export Citation
  • 12. P. Josefson K. Stralin A. Ohlin T. Ennefors B. Dragsten L. Andersson H. Fredlund P. Molling P. Olcen 2011 Evaluation of a commercial multiplex PCR test (SeptiFast) in the etiological diagnosis of community-onset bloodstream infections Eur J Clin Microbiol Infect Dis 30 1127 1134.

    • Search Google Scholar
    • Export Citation
  • 13. A. Heininger M. Binder A. Ellinger K. Botzenhart K. Unertl G. Doring 2003 DNase pretreatment of master mix reagents improves the validity of universal 16S rRNA gene PCR results J Clin Microbiol 41 1763 1765.

    • Search Google Scholar
    • Export Citation
  • 14. N. Wellinghausen A.J. Kochem C. Disque H. Muhl S. Gebert J. Winter J. Matten S.G. Sakka 2009 Diagnosis of bacteremia in whole-blood samples by use of a commercial universal 16S rRNA gene-based PCR and sequence analysis J Clin Microbiol 47 2759 2765.

    • Search Google Scholar
    • Export Citation
  • 15. C. Kuhn C. Disque H. Muhl P. Orszag M. Stiesch A. Haverich 2011 Evaluation of commercial universal rRNA gene PCR plus sequencing tests for identification of bacteria and fungi associated with infectious endocarditis J Clin Microbiol 49 2919 2923.

    • Search Google Scholar
    • Export Citation
  • 16. K. Grif I. Heller W.M. Prodinger K. Lechleitner C. Lass-Florl D. Orth 2012 Improvement of detection of bacterial pathogens in normally sterile body sites with a focus on orthopedic samples by use of a commercial 16S rRNA broad-range PCR and sequence analysis J Clin Microbiol 50 2250 2254.

    • Search Google Scholar
    • Export Citation
  • 17. D. Stubljar P. Rogina M. Skvarc A. Pavlovic 2013 Diagnostic accuracy of sCD14 (presepsin) is comparable to procalcitonin (PCT) for the diagnosis of bacterial infections in critically ill patients ECCMID Berlin.

    • Search Google Scholar
    • Export Citation
  • 18. O. Kommedal K. Kvello R. Skjastad N. Langeland H.G. Wiker 2009 Direct 16S rRNA gene sequencing from clinical specimens, with special focus on polybacterial samples and interpretation of mixed DNA chromatograms J Clin Microbiol 47 3562 3568.

    • Search Google Scholar
    • Export Citation
  • 19. O. Kommedal K. Lekang N. Langeland H.G. Wiker 2011 Characterization of polybacterial clinical samples using a set of group-specific broad-range primers targeting the 16S rRNA gene followed by DNA sequencing and RipSeq analysis J Med Microbiol 60 927 936.

    • Search Google Scholar
    • Export Citation
  • 20. S. Sachse E. Straube M. Lehmann M. Bauer S. Russwurm K.H. Schmidt 2009 Truncated human cytidylate-phosphate-deoxyguanylate-binding protein for improved nucleic acid amplification technique-based detection of bacterial species in human samples J Clin Microbiol 47 1050 1057.

    • Search Google Scholar
    • Export Citation
  • 21. C. Fitting M. Parlato M. Adib-Conquy N. Memain F. Philippart B. Misset M. Monchi J.M. Cavaillon C. Adrie 2012 DNAemia detection by multiplex PCR and biomarkers for infection in systemic inflammatory response syndrome patients PLoS One 7 e38916.

    • Search Google Scholar
    • Export Citation
  • 22. F. Bloos O. Bayer S. Sachse E. Straube K. Reinhart A. Kortgen 2013 Attributable costs of patients with candidemia and potential implications of polymerase chain reaction-based pathogen detection on antifungal therapy in patients with sepsis J Crit Care 28 2 8.

    • Search Google Scholar
    • Export Citation
  • 23. Schreiber J , Nierhaus A, Braune SA, de Heer G, Kluge S: Comparison of three different commercial PCR assays for the detection of pathogens in critically ill sepsis patients. Med Klin Intensivmed Notfmed (2013).

    • Search Google Scholar
    • Export Citation
  • 24. E. Leitner H.H. Kessler W. Spindelboeck M. Hoenigl C. Putz-Bankuti V. Stadlbauer-Kollner R. Krause A.J. Grisold G. Feierl R.E. Stauber 2013 Comparison of two molecular assays with conventional blood culture for diagnosis of sepsis J Microbiol Methods 92 253 255.

    • Search Google Scholar
    • Export Citation
  • 25. M. Ip T.H. Rainer N. Lee C. Chan S.S. Chau W. Leung M.F. Leung T.K. Tam G.E. Antonio G. Lui T.K. Lau D.S. Hui D. Fuchs R. Renneberg P.K. Chan 2007 Value of serum procalcitonin, neopterin, and C-reactive protein in differentiating bacterial from viral etiologies in patients presenting with lower respiratory tract infections Diagn Microbiol Infect Dis 59 131 136.

    • Search Google Scholar
    • Export Citation
  • 26. L. Simon F. Gauvin D.K. Amre P. Saint-Louis J. Lacroix 2004 Serum procalcitonin and C-reactive protein levels as markers of bacterial infection: a systematic review and metaanalysis Clin Infect Dis 39 206 217.

    • Search Google Scholar
    • Export Citation
  • 27. K. Reinhart M. Bauer N.C. Riedemann C.S. Hartog 2012 New approaches to sepsis: molecular diagnostics and biomarkers Clin Microbiol Rev 25 609 634.

    • Search Google Scholar
    • Export Citation
  • 28. P. Kopterides I. Tsangaris 2012 Procalcitonin and sepsis: recent data on diagnostic utility prognostic potential and therapeutic implications in critically ill patients Minerva Anestesiol 78 823 835.

    • Search Google Scholar
    • Export Citation
  • 29. R.A. Claus G.P. Otto H.P. Deigner M. Bauer 2010 Approaching clinical reality: markers for monitoring systemic inflammation and sepsis Curr Mol Med 10 227 235.

    • Search Google Scholar
    • Export Citation
  • 30. A. Au-Yong 2012 Towards evidence-based emergency medicine: best BETs from the Manchester Royal Infirmary. BET 2: C-reactive protein in the diagnosis of bacteraemia Emerg Med J 29 423 424.

    • Search Google Scholar
    • Export Citation
  • 31. S. Riedel J.H. Melendez A.T. An J.E. Rosenbaum J.M. Zenilman 2011 Procalcitonin as a marker for the detection of bacteremia and sepsis in the emergency department Am J Clin Pathol 135 182 189.

    • Search Google Scholar
    • Export Citation
  • 32. Y. Hirakata K. Yanagihara S. Kurihara K. Izumikawa M. Seki Y. Miyazaki S. Kohno 2008 Comparison of usefulness of plasma procalcitonin and C-reactive protein measurements for estimation of severity in adults with community-acquired pneumonia Diagn Microbiol Infect Dis 61 170 174.

    • Search Google Scholar
    • Export Citation
  • 33. P. Schuetz D.N. Amin J.L. Greenwald 2012 Role of procalcitonin in managing adult patients with respiratory tract infections Chest 141 1063 1073.

    • Search Google Scholar
    • Export Citation
  • 34. C.B. Chiwakata C. Manegold L. Bonicke I. Waase C. Julch M. Dietrich 2001 Procalcitonin as a parameter of disease severity and risk of mortality in patients with Plasmodium falciparum malaria J Infect Dis 183 1161 1164.

    • Search Google Scholar
    • Export Citation
  • 35. M. Jereb N.K. Lunacek T. Kotar A. Saksida M. Petrovec T. Avsic-Zupanc 2011 Procalcitonin in hantavirus infections Scand J Clin Lab Invest 71 287 291.

    • Search Google Scholar
    • Export Citation
  • 36. J. Koeze M.G. Hendrix F.A. van den Bergh R.M. Brouwer J.G. Zijlstra 2011 In critically ill patients the procalcitonin level can be misleading Crit Care 15 422.

    • Search Google Scholar
    • Export Citation
  • 37. B.H. Davis 2005 Improved diagnostic approaches to infection/ sepsis detection Expert Rev Mol Diagn 5 193 207.

  • 38. S.S. Qureshi S.M. Lewis V.A. Gant D. Treacher B.H. Davis K.A. Brown 2001 Increased distribution and expression of CD64 on blood polymorphonuclear cells from patients with the systemic inflammatory response syndrome (SIRS) Clin Exp Immunol 125 258 265.

    • Search Google Scholar
    • Export Citation
  • 39. J. Cid R. Aguinaco R. Sanchez G. Garcia-Pardo A. Llorente 2010 Neutrophil CD64 expression as marker of bacterial infection: a systematic review and meta-analysis J Infect 60 313 319.

    • Search Google Scholar
    • Export Citation
  • 40. Kaasch AJ , Rieg S, Kuetscher J, Brodt HR, Widmann T, Herrmann M, Meyer C, Welte T, Kern P, Haars U, Reuter S, Hubner I, Strauss R, Sinha B, Brunkhorst FM, Hellmich M, Fatkenheuer G, Kern WV, Seifert H: Delay in the administration of appropriate antimicrobial therapy in Staphylococcus aureus bloodstream infection: a prospective multicenter hospital-based cohort study. Infection (2013). Epub ahead of print.

    • Search Google Scholar
    • Export Citation
  • 41. M. Paolucci M. Stanzani F. Melchionda G. Tolomelli G. Castellani M.P. Landini S. Varani R.E. Lewis V. Sambri 2013 Routine use of a real-time polymerase chain reaction method for detection of bloodstream infections in neutropaenic patients Diagn Microbiol Infect Dis 75 130 134.

    • Search Google Scholar
    • Export Citation
  • 42. A. Mencacci C. Leli A. Cardaccia M. Meucci A. Moretti F. D'Alo S. Farinelli R. Pagliochini M. Barcaccia F. Bistoni 2012 Procalcitonin predicts real-time PCR results in blood samples from patients with suspected sepsis PLoS One 7 e53279.

    • Search Google Scholar
    • Export Citation
  • 43. M. Guido M. Quattrocchi A. Zizza G. Pasanisi V. Pavone G. Lobreglio G. Gabutti A. De Donno 2012 Molecular approaches in the diagnosis of sepsis in neutropenic patients with haematological malignances J Prev Med Hyg 53 104 108.

    • Search Google Scholar
    • Export Citation
  • 44. L. Pasqualini A. Mencacci C. Leli P. Montagna A. Cardaccia E. Cenci I. Montecarlo M. Pirro F. di Filippo E. Cistaro G. Schillaci F. Bistoni E. Mannarino 2012 Diagnostic performance of a multiple real-time PCR assay in patients with suspected sepsis hospitalized in an internal medicine ward J Clin Microbiol 50 1285 1288.

    • Search Google Scholar
    • Export Citation
  • 45. P.M. Rath F. Saner A. Paul N. Lehmann E. Steinmann J. Buer J. Steinmann 2012 Multiplex PCR for rapid and improved diagnosis of bloodstream infections in liver transplant recipients J Clin Microbiol 50 2069 2071.

    • Search Google Scholar
    • Export Citation
  • 46. U. Lodes H. Lippert F. Meyer 2011 Molecular biological sepsis diagnostic using multiplex PCR in surgical intensive care as suitable alternative to conventional microbial culture — a representative overview Zentralbl Chir 136 135 142.

    • Search Google Scholar
    • Export Citation
  • 47. D. Bravo J. Blanquer M. Tormo G. Aguilar R. Borras C. Solano M.A. Clari E. Costa B. Munoz-Cobo M. Argueso J.R. Pineda D. Navarro 2011 Diagnostic accuracy and potential clinical value of the LightCycler SeptiFast assay in the management of bloodstream infections occurring in neutropenic and critically ill patients Int J Infect Dis 15 e326 331.

    • Search Google Scholar
    • Export Citation
  • 48. D. Maubon R. Hamidfar-Roy S. Courby A. Vesin M. Maurin P. Pavese N. Ravanel C.E. Bulabois J.P. Brion H. Pelloux J.F. Timsit 2010 Therapeutic impact and diagnostic performance of multiplex PCR in patients with malignancies and suspected sepsis J Infect 61 335 342.

    • Search Google Scholar
    • Export Citation
  • 49. Yanagihara K , Kitagawa Y, Tomonaga M, Tsukasaki K, Kohno S, Seki M, Sugimoto H, Shimazu T, Tasaki O, Matsushima A, Ikeda Y, Okamoto S, Aikawa N, Hori S, Obara H, Ishizaka A, Hasegawa N, Takeda J, Kamihira S, Sugahara K, Asari S, Murata M, Kobayashi Y, Ginba H, Sumiyama Y, Kitajima M: Evaluation of pathogen detection from clinical samples by real-time polymerase chain reaction using a sepsis pathogen DNA detection kit. Crit Care 14, R159 (2010).

    • Search Google Scholar
    • Export Citation
  • 50. M. Avolio P. Diamante S. Zamparo M.L. Modolo S. Grosso P. Zigante N. Tosoni R. De Rosa P. Stano A. Camporese 2010 Molecular identification of bloodstream pathogens in patients presenting to the emergency department with suspected sepsis Shock 34 27 30.

    • Search Google Scholar
    • Export Citation
  • 51. S. Varani M. Stanzani M. Paolucci F. Melchionda G. Castellani L. Nardi M.P. Landini M. Baccarani A. Pession V. Sambri 2009 Diagnosis of bloodstream infections in immunocompromised patients by real-time PCR J Infect 58 346 351.

    • Search Google Scholar
    • Export Citation
  • 52. D.C. Kasper I. Altiok T.P. Mechtler J. Bohm J. Straub M. Langgartner A. Pollak K.R. Herkner A. Berger 2013 Molecular detection of late-onset neonatal sepsis in premature infants using small blood volumes: proof-of-concept Neonatology 103 268 273.

    • Search Google Scholar
    • Export Citation
  • 53. E. Tschiedel J. Steinmann J. Buer J.G. Onnebrink U. Felderhoff-Muser P.M. Rath C. Dohna-Schwake 2012 Results and relevance of molecular detection of pathogens by SeptiFast — a retrospective analysis in 75 critically ill children Klin Padiatr 224 12 16.

    • Search Google Scholar
    • Export Citation
  • 54. Torres-Martos E , Perez-Ruiz M, Pedrosa-Corral I, Pena-Caballero M, Jimenez-Valera MM, Perez-Ramirez MD, Navarro-Mari JM: Evaluation of the LightCycler((R)) Septi-Fast test in newborns and infants with clinical suspicion of sepsis. Enferm Infecc Microbiol Clin (2012).

    • Search Google Scholar
    • Export Citation
  • 55. B. Lucignano S. Ranno O. Liesenfeld B. Pizzorno L. Putignani P. Bernaschi D. Menichella 2011 Multiplex PCR allows rapid and accurate diagnosis of bloodstream infections in newborns and children with suspected sepsis J Clin Microbiol 49 2252 2258.

    • Search Google Scholar
    • Export Citation
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Senior editors

Editor(s)-in-Chief: Dunay, Ildiko Rita, Prof. Dr. Pharm, Dr. rer. nat., University of Magdeburg, Germany

Editor(s)-in-Chief: Heimesaat, Markus M., Prof. Dr. med., Charité - University Medicine Berlin, Germany

Editorial Board

  • Berit Bangoura, Dr. DVM. PhD,  University of Wyoming, USA
  • Stefan Bereswill, Prof. Dr. rer. nat., Charité - University Medicine Berlin, Germany
  • Dunja Bruder, Prof. Dr. rer. nat., University of Magdeburg, Germany
  • Jan Buer, Prof. Dr. med., University of Duisburg, Germany
  • Edit Buzas, Prof. Dr. med., Semmelweis University, Hungary
  • Renato Damatta, Prof. PhD, UENF, Brazil
  • Maria Deli, MD, PhD, DSc, Biological Research Center, HAS, Hungary
  • Olgica Djurković-Djaković, Prof. Phd, University of Belgrade, Serbia
  • Jean-Dennis Docquier, Prof. Dr. med., University of Siena, Italy
  • Zsuzsanna Fabry, Prof. Phd, University of Washington, USA
  • Ralf Ignatius, Prof. Dr. med., Charité - University Medicine Berlin, Germany
  • Achim Kaasch, Prof. Dr. med., Otto von Guericke University Magdeburg, Germany
  • Oliver Liesenfeld, Prof. Dr. med., Inflammatix, USA
  • Matyas Sandor, Prof. PhD, University of Wisconsin, USA
  • Ulrich Steinhoff, Prof. PhD, University of Marburg, Germany
  • Michal Toborek, Prof. PhD, University of Miami, USA
  • Susanne A. Wolf, PhD, MDC-Berlin, Germany

 

Dr. Dunay, Ildiko Rita
Magdeburg, Germany
E-mail: ildiko.dunay@med.ovgu.de

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2023  
Web of Science  
Total Cites
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674
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Immunology (Q3)
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Scopus
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Microbiology (medical) Q2
Scopus
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0.832

 

European Journal of Microbiology and Immunology
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European Journal of Microbiology and Immunology
Language English
Size A4
Year of
Foundation
2011
Volumes
per Year
1
Issues
per Year
4
Founder Akadémiai Kiadó
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ISSN 2062-509X (Print)
ISSN 2062-8633 (Online)

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