Authors:
,
,
,
, and
GL Nádasy
GL Nádasy
Semmelweis University, Hungary
Semmelweis University, Hungary
Search for other papers by GL Nádasy in
Current site
Google Scholar
PubMed
Semmelweis University, Hungary
Search for other papers by GL Nádasy in
Current site
Google Scholar
PubMed
G Raffai
G Raffai
Semmelweis University, Hungary
Search for other papers by G Raffai in
Current site
Google Scholar
PubMed
Search for other papers by G Raffai in
Current site
Google Scholar
PubMed
E Fehér
E Fehér
Semmelweis University, Hungary
Search for other papers by E Fehér in
Current site
Google Scholar
PubMed
Search for other papers by E Fehér in
Current site
Google Scholar
PubMed
G Schaming
G Schaming
Semmelweis University, Hungary
Search for other papers by G Schaming in
Current site
Google Scholar
PubMed
Search for other papers by G Schaming in
Current site
Google Scholar
PubMed
E Monos
E Monos
Semmelweis University, Hungary
Search for other papers by E Monos in
Current site
Google Scholar
PubMed
Search for other papers by E Monos in
Current site
Google Scholar
PubMed
Aims
Labyrinthectomized rats are suitable models to test consequences of vestibular lesion and are widely used to study neural plasticity. We describe a combined microsurgical–chemical technique that can be routinely performed with minimum damage.
Methods
Caudal leaflet of the parotis is elevated. The tendinous fascia covering the bulla is opened frontally from the sternomastoid muscle’s tendon while sparing facial nerve branches. A 4 mm diameter hole is drilled into the bulla’s hind lower lateral wall to open the common (in rodents) mastoid-tympanic cavity. The cochlear crista (promontory) at the lower posterior part of its medial wall is identified as a bony prominence. A 1 mm diameter hole is drilled into its lower part. The perilymphatic/endolymphatic fluids with tissue debris of the Corti organ are suctioned. Ethanol is injected into the hole. Finally, 10 µL of sodium arsenite solution (50 µM/mL) is pumped into the labyrinth and left in place for 15 min. Simple closure in two layers (fascia and skin) is sufficient.
Results and conclusion
All rats had neurological symptoms specific for labyrinthectomy (muscle tone, body position, rotatory movements, nystagmus, central deafness). Otherwise, their behavior was unaffected, drinking and eating normally. After a few days, they learned to balance relying on visual and somatic stimuli (neuroplasticity).
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Bergquist F , Ruthven A , Ludwig M , Dutia MB : Histaminergic and glycinergic modulation of GABA release in the vestibular nuclei of normal and labyrinthectomised rats. J. Physiol. 577, 857–868 (2006)
-
2.
Cameron SA , Dutia MB : Cellular basis of vestibular compensation: changes in intrinsic excitability of MVN neurons. Neuroreport 8, 2595–2599 (1997)
-
3.
Campos-Torres A , Vidal PP , de Waele C : Evidence for a microglial reaction within the vestibular and cochlear nuclei following inner ear lesion in the rat. Neuroscience 92, 1475–1490 (1999)
-
4.
Cirelly C , Pompeiano M , D’Ascani P , Arrighi P , Pompeiano O : C-fos expression in the rat brain after unilateral labyrinthectomy and its relation to the uncompensated and compensated stages. Neuroscience 70, 515–546 (1996)
-
5.
Curthoys IS : Vestibular compensation and substitution. Curr. Opin. Neurol. 13, 27–30 (2000)
-
6.
Deák A , Bácskai T , Gaál B , Rácz E , Matesz K : Effect of unilateral labyrinthectomy on the molecular composition of perineuronal nets in the lateral vestibular nucleus of the rat. Neurosci. Lett. 513, 1–5 (2012)
-
7.
De Waele C , Abitbol M , Chat M , Menini C , Mallet J , Vidal PP : Distribution of glutamatergic receptors and GAD mRNA-containing neurons in the vestibular nuclei of normal and hemilabyrinthectomized rats. Eur. J. Neurosci. 6, 565–576 (1994)
-
8.
Di Filippo M , Tozzi A , Costa C , Belcastro V , Tantucci M , Picconi B , Calabresi P : Plasticity and repair in the post-ischemic brain. Neuropharmacology 55, 353–362 (2008)
-
9.
Fukushima M , Kitahara T , Takeda N , Saika T , Uno A , Kubo T : Role of cholinergic mossy fibers in medial vestibular and prepositus hypoglossal nuclei in vestibular compensation. Neuroscience 102, 159–166 (2001)
-
10.
Goto MM , Romero GG , Balaban CD : Transient changes in flocculonodular lobe protein kinase C expression during vestibular compensation. J. Neurosci. 17, 4367–4381 (1997)
-
11.
Hitier M , Besnard S , Vignaux G , Denise P , Moreau S : The ventrolateral surgical approach to labyrinthectomy in rats: anatomical description and clinical consequences. Surg. Radiol. Anat. 32, 835–842 (2010)
-
12.
Horii A , Smith PF , Darlington CL : Quantitative changes in gene expression of glutamate receptor subunits/subtypes in the vestibular nucleus, inferior olive and flocculus before and following unilateral labyrinthectomy in the rat: real-time quantitative PCR method. Exp. Brain Res. 139, 188–200 (2001)
-
13.
Kasri M , Picqet F , Falempin M : Effects of unilateral and bilateral labyrinthectomy on rat postural muscle properties: the soleus. Exp. Neurol. 185, 143–153 (2004)
-
14.
Kim MS , Jin BK , Chun SW , Lee MY , Lee S H , Kim JH , Park BR : Role of vestibulocerebellar N-methylaspartate receptors for behavioral recovery following unilateral labyrinthectomy in rats. Neurosci. Lett. 222, 171–174 (1997)
-
15.
Kitahara T , Takeda N , Kubo T , Kiyama H : An implication of protein phosphatase 2A-beta in the rat flocculus for lesion-induced vestibular plasticity. Acta Otolaryngol. 118, 685–691 (1998)
-
16.
Kitahara T , Takeda N , Saika T , Kubo T , Kiyama H : Effects of MK801 on Fos expression in the rat brainstem after unilateral labyrinthectomy. Brain Res. 700, 182–190 (1995)
-
17.
Lozada AF , Aarnisalo AA , Karlstedt K , Stark H , Panula P : Plasticity of histamine H3 receptor expression and binding in the vestibular nuclei after labyrinthectomy in rat. BMC Neurosci. 5, 32 (2004)
-
18.
Matsuda T , Gotoh TM , Tanaka K , Gao S , Morita H : Vestibulosympathetic reflex mediates the pressor response to hypergravity in conscious rats: contribution of the diencephalon. Brain Res. 102, 140–147 (2004)
-
19.
Monos E , Raffai G , Dörnyei G , Nádasy G , Fehér E : Structural and functional responses of extremity veins to long-term gravitational loading and unloading, lessons from animal systems. Acta Astronaut. 60, 406–414 (2007)
-
20.
Park BR , Choi MA , Hong SM : Temporal changes of calbindin expression in the nodulus following unilateral labyrinthectomy in rats. Neurosci. Lett. 555, 47–50 (2013)
-
21.
Paterson JM , Short D , Flatman PW , Seckl JR , Aitken A , Dutia MB : Changes in protein expression in the rat medial vestibular nuclei during vestibular compensation. J. Physiol. 575, 777–788 (2006)
-
22.
Russell NA , Horii A , Smith PF , Darlington CL , Bilkey DK : Long-term effects of permanent vestibular lesions on hippocampal spatial firing. J. Neurosci. 23, 6490–6498 (2003)
-
23.
Vignaux G , Chabbert C , Gaboyard-Niay S , Travo C , Machado ML , Denise P , Comoz F , Hitier M , Landemore G , Philoxène B , Besnard S : Evaluation of the chemical model of vestibular lesions induced by arsanilate in rats. Toxicol. Appl. Pharmacol. 258, 61–71 (2012)
-
24.
Yamanaka T , Him A , Cameron SA , Dutia MB : Rapid compensatory changes in GABA receptor efficacy in rat vestibular neurones after unilateral labyrinthectomy. J. Physiol. 523, 413–424 (2000)
-
25.
Zheng Y , Horii A , Appleton I , Darlington CL , Smith PF : Damage to the vestibular inner ear causes long-term changes in neuronal nitric oxide synthase expression in the rat hippocampus. Neuroscience 105, 1–5 (2001)
Editor-in-Chief
László ROSIVALL (Semmelweis University, Budapest, Hungary)
Managing Editor
Anna BERHIDI (Semmelweis University, Budapest, Hungary)
Co-Editors
- Gábor SZÉNÁSI (Semmelweis University, Budapest, Hungary)
- Ákos KOLLER (Semmelweis University, Budapest, Hungary)
- Zsolt RADÁK (University of Physical Education, Budapest, Hungary)
- László LÉNÁRD (University of Pécs, Hungary)
- Zoltán UNGVÁRI (Semmelweis University, Budapest, Hungary)
Assistant Editors
- Gabriella DÖRNYEI (Semmelweis University, Budapest, Hungary)
- Zsuzsanna MIKLÓS (Semmelweis University, Budapest, Hungary)
- György NÁDASY (Semmelweis University, Budapest, Hungary)
Hungarian Editorial Board
- György BENEDEK (University of Szeged, Hungary)
- Zoltán BENYÓ (Semmelweis University, Budapest, Hungary)
- Mihály BOROS (University of Szeged, Hungary)
- László CSERNOCH (University of Debrecen, Hungary)
- Magdolna DANK (Semmelweis University, Budapest, Hungary)
- László DÉTÁRI (Eötvös Loránd University, Budapest, Hungary)
- Zoltán GIRICZ (Semmelweis University, Budapest, Hungary and Pharmahungary Group, Szeged, Hungary)
- Zoltán HANTOS (Semmelweis University, Budapest and University of Szeged, Hungary)
- Zoltán HEROLD (Semmelweis University, Budapest, Hungary)
- László HUNYADI (Semmelweis University, Budapest, Hungary)
- Gábor JANCSÓ (University of Pécs, Hungary)
- Zoltán KARÁDI (University of Pecs, Hungary)
- Miklós PALKOVITS (Semmelweis University, Budapest, Hungary)
- Gyula PAPP (University of Szeged, Hungary)
- Gábor PAVLIK (University of Physical Education, Budapest, Hungary)
- András SPÄT (Semmelweis University, Budapest, Hungary)
- Gyula SZABÓ (University of Szeged, Hungary)
- Zoltán SZELÉNYI (University of Pécs, Hungary)
- Lajos SZOLLÁR (Semmelweis University, Budapest, Hungary)
- József TOLDI (MTA-SZTE Neuroscience Research Group and University of Szeged, Hungary)
- Árpád TÓSAKI (University of Debrecen, Hungary)
International Editorial Board
- Dragan DJURIC (University of Belgrade, Serbia)
- Christopher H. FRY (University of Bristol, UK)
- Stephen E. GREENWALD (Blizard Institute, Barts and Queen Mary University of London, UK)
- Tibor HORTOBÁGYI (University of Groningen, Netherlands)
- George KUNOS (National Institutes of Health, Bethesda, USA)
- Massoud MAHMOUDIAN (Iran University of Medical Sciences, Tehran, Iran)
- Tadaaki MANO (Gifu University of Medical Science, Japan)
- Luis Gabriel NAVAR (Tulane University School of Medicine, New Orleans, USA)
- Hitoo NISHINO (Nagoya City University, Japan)
- Ole H. PETERSEN (Cardiff University, UK)
- Ulrich POHL (German Centre for Cardiovascular Research and Ludwig-Maximilians-University, Planegg, Germany)
- Andrej A. ROMANOVSKY (University of Arizona, USA)
- Anwar Ali SIDDIQUI (Aga Khan University, Karachi, Pakistan)
- Csaba SZABÓ (University of Fribourg, Switzerland)
- Eric VICAUT (Université de Paris, UMRS 942 INSERM, France)
Editorial Correspondence:
Physiology International
Semmelweis University
Faculty of Medicine, Institute of Translational Medicine
Nagyvárad tér 4, H-1089 Budapest, Hungary
Phone/Fax: +36-1-2100-100
E-mail: pi@semmelweis.hu
Indexing and Abstracting Services:
- Biological Abstracts
- BIOSIS Previews
- CAB Abstracts
- CABELLS Journalytics
- EMBASE/Excerpta Medica
- Global Health
- Index Copernicus
- Index Medicus
- Medline
- Referativnyi Zhurnal
- SCOPUS
- WoS - Science Citation Index Expanded
| 2024 | |
|---|---|
| Scopus | |
| CiteScore | |
| CiteScore rank | |
| SNIP | |
| Scimago | |
| SJR index | 0.356 |
| SJR Q rank | Q2 |
| 2023 | |
|---|---|
| Web of Science | |
| Journal Impact Factor | 2.2 |
| Rank by Impact Factor | Q3 (Physiology) |
| Journal Citation Indicator | 0.58 |
| Scopus | |
| CiteScore | 3.4 |
| CiteScore rank | Q2 (Physical Therapy, Sports Therapy and Rehabilitation) |
| SNIP | 0.508 |
| Scimago | |
| SJR index | 0.407 |
| SJR Q rank | Q2 |
| Physiology International | |
|---|---|
| Publication Model | Hybrid |
| Submission Fee | none |
| Article Processing Charge | Effective from 1st Apr 2025: 600 EUR/article |
| Printed Color Illustrations | 40 EUR (or 10 000 HUF) + VAT / piece |
| Regional discounts on country of the funding agency | World Bank Lower-middle-income economies: 50% World Bank Low-income economies: 100% |
| Further Discounts | Editorial Board / Advisory Board members: 50% Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100% |
| Subscription fee 2025 | Online subsscription: 752 EUR / 828 USD Print + online subscription: 880 EUR / 968 USD |
| Subscription Information | Online subscribers are entitled access to all back issues published by Akadémiai Kiadó for each title for the duration of the subscription, as well as Online First content for the subscribed content. |
| Purchase per Title | Individual articles are sold on the displayed price. |
| Physiology International | |
|---|---|
| Language | English |
| Size | B5 |
| Year of Foundation |
2006 (1950) |
| Volumes per Year |
1 |
| Issues per Year |
4 |
| Founder | Magyar Tudományos Akadémia |
| Founder's Address |
H-1051 Budapest, Hungary, Széchenyi István tér 9. |
| Publisher | Akadémiai Kiadó |
| Publisher's Address |
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. |
| Responsible Publisher |
Chief Executive Officer, Akadémiai Kiadó |
| ISSN | 2498-602X (Print) |
| ISSN | 2677-0164 (Online) |
Monthly Content Usage
| Abstract Views | Full Text Views | PDF Downloads | |
|---|---|---|---|
| Dec 2024 | 25 | 0 | 0 |
| Jan 2025 | 40 | 1 | 2 |
| Feb 2025 | 45 | 0 | 0 |
| Mar 2025 | 49 | 0 | 0 |
| Apr 2025 | 22 | 0 | 0 |
| May 2025 | 15 | 0 | 0 |
| Jun 2025 | 0 | 0 | 0 |
Authors:
, , , , , , and
Copyright Akadémiai Kiadó AKJournals is the trademark of Akadémiai Kiadó's journal publishing business branch.
Powered by PubFactory