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  • 1 European University of Madrid, Spain
  • 2 Polytechnic University of Madrid, Spain
  • 3 University of Granada, Spain
  • 4 University of Granada, Spain
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This study was aimed to analyze the effect of two different megadoses of α-tocopherol (vit E) in the antioxidant activity and red and white blood series of Wistar rats after a 180-min ultraendurance probe. Three groups of 10 rats were analyzed; VEAG: acute administration of a megadoses of 5,000 IU/kg of vit E the day before the probe; VECG: chronic administration of 1,000 IU/kg/day of vit E for 6 days before the probe; CG: placebo administration. VEAG presented white cells, red blood cells, hematocrit, hemoglobin values significantly higher than CG and VECG (p < 0.05). The mean corpuscular hemoglobin and lymphocytes concentrations were significantly higher in the VECG than in the other two groups (p < 0.05). Similarly, VEAG presented a significantly higher vit E blood concentration than VECG and CG (p < 0.05), and VECG than CG (p < 0.05). Finally, we found a significantly positive correlation between trolox equivalent antioxidant capacity (TEAC) and red blood cells concentration (r = 0.374) and a significantly inverse correlation between TEAC and blood lactate concentration (r = −0.365). Our findings suggest that acute vit E megadoses could protect against transitory sport anemia symptoms and increase the white blood cell count in comparison with the chronic dose and control groups after an ultraendurance probe.

  • 1.

    Aguilo A , Tauler P , Fuentespina E , Villa G , Cordova A , Tur JA , Pons A : Antioxidant diet supplementation influences blood iron status in endurance athletes. Int. J. Sport Nutr. Exerc. Metab. 14, 147160 (2004)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 2.

    Baskurt OK , Meiselman HJ : Hemodynamic effects of red blood cell aggregation. Indian J. Exp. Biol. 45, 2531 (2007)

  • 3.

    Berzosa C , Gómez-Trullén EM , Piedrafita E , Cebrián I , Martínez-Ballarín E , Miana-Mena FJ , Fuentes-Broto L , García JJ : Erythrocyte membrane fluidity and indices of plasmatic oxidative damage after acute physical exercise in humans. Eur. J. Appl. Physiol. 111, 11271133 (2011)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 4.

    Bompadre S , Leone L , Politi A , Battino M : Improved FIA-ABTS method for antioxidant capacity determination in different biological samples. Free Rad. Res. 38, 831838 (2004)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 5.

    Clemente-Suárez VJ : Changes in biochemical, strength, flexibility and aerobic capacity parameters after a 1700 km ultraendurance cycling race. BioMed Res. Int. 2014, 602620 (2014)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 6.

    Hale JP , Winlove CP , Petrov PG : Effect of hydroperoxides on red blood cell membrane mechanical properties. Biophys. J. 101, 19211929 (2011)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 7.

    Jenkins RR , Goldfarb A : Introduction: Oxidant stress, aging, and exercise. Med. Sci. Sports Exerc. 25, 210212 (1993)

  • 8.

    Kłapcińska B , Waśkiewicz Z , Chrapusta SJ , Sadowska-Krępa E , Czuba M , Langfort J : Metabolic responses to a 48-h ultra-marathon run in middle-aged male amateur runners. Eur. J. Appl. Physiol. 113, 27812793 (2013)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 9.

    MacCrehan WA : Determination of retinol, α-tocopherol and β-carotene in serum by liquid chromatography. Methods Enzymol. 189, 172181 (1990)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 10.

    Marsh SA , Laursen PB , Coombes JS : Effects of antioxidant supplementation and exercise training on erythrocyte antioxidant enzymes. Int. J. Vitam. Nutr. Res. 76, 324331 (2006)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 11.

    Martín S , Nebot E , Clemente V , Aparicio V , Kapravelou G , Porres J , Urbano G , Aranda P : Eficacia de dos protocolos incrementales hasta alcanzar el VO2max en ratas wistar [Efficiency of two incremental protocols until reaching VO2max in Wistar rats]. Arch. Med. Deporte. 139, 400 (2010)

    • Search Google Scholar
    • Export Citation
  • 12.

    Mastaloudis A , Leonard SW , Traber MG : Oxidative stress in athletes during extreme endurance exercise. Free Radic. Biol. Med. 31, 911922 (2001)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 13.

    Maughan RJ : Fluid and electrolyte loss and replacement in exercise. J. Sports Sci. 9, 117142 (1991)

  • 14.

    McAnulty SR , McAnulty LS , Nieman DC , Morrow JD , Shooter LA , Holmes S , Heward C , Henson DA : Effect of alpha-tocopherol supplementation on plasma homocysteine and oxidative stress in highly trained athletes before and after exhaustive exercise. J. Nutr. Biochem. 16, 530537 (2005)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 15.

    Merry TL , Ristow M : Do antioxidant supplements interfere with skeletal muscle adaptation to exercise training? J. Physiol. 594, 51355147 (2016)

  • 16.

    Miller ER , Pastor-Barriuso R , Dalal D , Riemersma RA , Appel LJ , Guallar E : Meta-analysis: High-dosage vitamin E supplementation may increase all-cause mortality. Ann. Intern. Med. 142, 3746 (2005)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 17.

    Miyazaki H , Oh-ishi S , Ookawara T , Kizaki T , Toshinai K , Ha S , Haga S , Ji LL , Ohno H :Strenuous endurance training in humans reduces oxidative stress following exhausting exercise. Eur. J. Appl. Physiol. 84, 16 (2001)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 18.

    Monsen ER : Dietary reference intakes for the antioxidant nutrients: Vitamin C, vitamin E, selenium, and carotenoids. J. Am. Diet. Assoc. 100, 637640 (2000)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 19.

    Mooren FC , Lechtermann A , Volker K : Exercise-induced apoptosis of lymphocytes depends on training status. Med. Sci. Sports Exerc. 36, 14761483 (2004)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 20.

    Patil SM , Chaudhuri D , Dhanakshirur GB : Role of alpha-tocopherol in cardiopulmonary fitness in endurance athletes, cyclists. Indian J. Physiol. Pharmacol. 53, 375379 (2009)

    • Search Google Scholar
    • Export Citation
  • 21.

    Paulsen G , Cumming KT , Holden G , Hallén J , Rønnestad BR , Sveen O , Skaug A , Paur I , Bastani NE , Østgaard HN , Buer C , Midttun M , Freuchen F , Wiig H , Ulseth ET , Garthe I , Blomhoff R , Benestad HB , Raastad T : Vitamin C and E supplementation hampers cellular adaptation to endurance training in humans: A double-blind, randomised, controlled trial. J. Physiol. 592, 18871901 (2014)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 22.

    Radak Z , Ishihara K , Tekus E , Varga C , Posa A , Balogh L , Boldogh I , Koltai E : Exercise, oxidants, and antioxidants change the shape of the bell-shaped hormesis curve. Redox Biol. 12, 285290 (2017)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 23.

    Robinson Y , Cristancho E , Boning D : Intravascular hemolysis and mean red blood cell age in athletes. Med. Sci. Sports Exerc. 38, 480483 (2006)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 24.

    Rowlands DS , Pearce E , Aboud A , Gillen JB , Gibala MJ , Donato S , Waddington JM , Green JG , Tarnopolsky MA : Oxidative stress, inflammation, and muscle soreness in an 894-km relay trail run. Eur. J. Appl. Physiol. 112, 18391848 (2012)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 25.

    Schmidt W , Heinicke K , Rojas J , Manuel Gomez J , Serrato M , Mora M , Wolfarth B , Schmid A , Keul J : Blood volume and hemoglobin mass in endurance athletes from moderate altitude. Med. Sci. Sports Exerc. 34, 19341940 (2002)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 26.

    Sentürk UK , Gündüz F , Kuru O , Aktekin MR , Kipmen D , Yalçin O , Bor-Küçükatay M , Yeşilkaya A , Başkurt OK : Exercise-induced oxidative stress affects erythrocytes in sedentary rats but not exercise-trained rats. J. Appl. Physiol. 91, 19992004 (2001)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 27.

    Sentürk UK , Gündüz F , Kuru O , Koçer G , Ozkaya YG , Yesilkaya A , Bor-Küçükatay M , Uyüklü M , Yalçin O , Baskurt OK : Exercise-induced oxidative stress leads hemolysis in sedentary but not trained humans. J. Appl. Physiol. 99, 14341441 (2005)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 28.

    Smith JA , Kolbuch-Braddon M , Gillam I , Telford RD , Weidemann MJ : Changes in the susceptibility of red blood cells to oxidative and osmotic stress following submaximal exercise. Eur. J. Appl. Physiol. Occup. Physiol. 70, 427436 (1995)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 29.

    Stäubli M , Roessler B : The mean red cell volume in long distance runners. Eur. J. Appl. Physiol. Occup. Physiol. 55, 4953 (1986)

  • 30.

    Taber M , Stevens J : Vitamins C and E: Beneficial effects from a mechanistic perspective. Free Rad. Biol. Med. 51, 10001013 (2011)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 31.

    Tain YL , Freshour G , Dikalova A , Griendling K , Baylis C : Vitamin E reduces glomerulosclerosis, restores renal neuronal NOS, and suppresses oxidative stress in the 5/6 nephrectomized rat. Am. J. Physiol. Renal. Physiol. 292, F1404F1410 (2007)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 32.

    Traber MG : Relationship of vitamin E metabolism and oxidation in exercising human subjects. Br. J. Nutr. 96, S34S37 (2006)

  • 33.

    Traber MG , Frei B , Beckman J : Vitamin E revisited: Do new data validate benefits for chronic disease prevention? Curr. Opin. Lipidol. 19, 3038 (2008)

    • Search Google Scholar
    • Export Citation
  • 34.

    Urso ML , Clarkson PM : Oxidative stress, exercise, and antioxidant supplementation. Toxicology 189, 4154 (2003)

  • 35.

    Viitala P , Newhouse I : Vitamin E supplementation, exercise and lipid peroxidation in human participants. Eur. J. Appl. Physiol. 93, 108115 (2004)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 36.

    Waśkiewicz Z , Kłapcińska B , Sadowska-Krępa E , Czuba M , Kempa K , Kimsa E , Gerasimuk D : Acute metabolic responses to a 24-h ultra-marathon race in male amateur runners. Eur. J. Appl. Physiol. 112, 16791688 (2012)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 37.

    Wu H , Chen K , Shee B , Chang H , Huang Y , Yang R : Effects of 24 h ultra-marathon on biochemical and hematological parameters. World J. Gastroenterol. 10, 27112714 (2004)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 38.

    Zembron-Lacny A , Szyszka K , Sobanska B , Pakula R : Prooxidant-antioxidant equilibrium in rowers: Effect of a single dose of vitamin E. J. Sports Med. Phys. Fitness 46, 257264 (2006)

    • Search Google Scholar
    • Export Citation

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