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  • 1 Nagasaki University Graduate School of Biomedical Sciences, Japan
  • 2 Nagasaki Memorial Hospital, Japan
  • 3 Nagasaki University Graduate School of Biomedical Sciences, Japan
  • 4 Nagasaki Genbaku Hospital, Japan
  • 5 Nagasaki University Hospital, Japan
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This study examined the aging effect on disuse muscle atrophy prevention using heat stress. Wistar rats aged 7 and 60 weeks were divided into three groups as follows: control, immobilized (Im), and immobilized and heat stressed (ImH). Heat stress was given by immersing the hindlimbs in hot water (42 °C) for 60 min, once in every 3 days and the gastrocnemius (GAS) and soleus (SOL) muscles were extracted after 14 days. Muscle-fiber types were classified using ATPase staining. Heat shock protein 70 (HSP70) was assessed through Western blotting. In GAS muscle of both groups and SOL muscle of 7-week-old rats, the fiber diameter of each muscle type in the ImH group significantly increased compared with that in the Im group. However, this could not be observed in the SOL muscle of the 60-week-old rats. The increased percentage of type-I fibers and variability of types I and II muscle-fiber diameter were evident in the SOL muscle of the 60-week rats. HSP70 was significantly elevated in the ImH group compared with in the Im group in both muscle types of both age groups. Thus, effectiveness of heat stress in the prevention of disuse muscle atrophy appears unsatisfactory in aging muscle fibers.

  • 1.

    Ansved T : Effects of immobilization on the rat soleus muscle in relation to age. Acta Physiol. Scand. 154, 291302 (1995)

  • 2.

    Brooks SV , Faulkner JA : Skeletal muscle weakness in old age: underlying mechanisms. Med. Sci. Sports. Exerc. 26, 432439 (1994)

  • 3.

    Delp MD , Duan C : Composition and size of type I, IIA, IID/X, and IIB fibers and citrate synthase activity of rat muscle. J. Appl. Physiol. 80, 261270 (1996)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 4.

    Eddinger TJ , Moss RL , Cassens RG : Fiber number and type composition extensor digitorum longus, soleus, and diaphragm muscles with aging in Fisher 344 rats. J. Histochem. Cytochem. 33, 10331041 (1985)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 5.

    Goto K , Okuyama R , Sugiyama H , Honda M , Kobayashi T , Uehara K , Akema T , Sugiura T , Yamada S , Ohira Y , Yoshioka T : Effects of heat stress and mechanical stretch on protein expression in cultured skeletal muscle cells. Pflugers. Arch. 447, 247253 (2003)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 6.

    Husom AD , Ferrington DA , Thompson LV : Age-related differences in the adaptive potential of type I skeletal muscle fibers. Exp. Gerontol. 40, 227235 (2005)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 7.

    Kataoka H , Okita M , Nakai K , Toyoda N , Yoshikawa S , Watanabe Y , Nakano J : Effects of heat stress on prevention of disuse muscle atrophy in rat skeletal muscle. Rigakuryohougaku 31, 331336 (2004)

    • Search Google Scholar
    • Export Citation
  • 8.

    Kondo H , Nakagaki I , Sasaki S , Hori S , Itokawa Y : Mechanism of oxidative stress in skeletal muscle atrophied by immobilization. Am. J. Physiol. 265, E839E844 (1993)

    • Search Google Scholar
    • Export Citation
  • 9.

    Locke M : Heat shock transcription factor activation and Hsp72 accumulation in aged skeletal muscle. Cell Stress Chaperones 5, 4551 (2000)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 10.

    Morimoto Y , Kondo Y , Kataoka H , Honda Y , Kozu R , Sakamoto J , Nakano J , Origuchi T , Yoshimura T , Okita M : Heat treatment inhibits skeletal muscle atrophy of glucocorticoid-induced myopathy in rats. Physiol. Res. 64(6), 897905 (2015)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 11.

    Naito H , Powers SK , Demirel HA , Aoki J : Exercise training increases heat shock protein in skeletal muscle of old rats. Med. Sci. Sports Exerc. 33, 729734 (2001)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 12.

    Naito H , Powers SK , Demirel HA , Sugiura T , Dodd SL , Aoki J : Heat stress attenuates skeletal muscle atrophy in hindlimb-unweighted rats. J. Appl. Physiol. 88, 359363 (2000)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 13.

    Oishi Y , Taniguchi K , Matsumoto H , Ishihara A , Ohira Y , Roy RR : Differential response of HSPs to heat stress in slow and fast regions of rat gastrocnemius muscle. Muscle Nerve 28, 587594 (2003)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 14.

    Oishi Y , Taniguchi K , Matsumoto H , Ishihara A , Ohira Y , Roy RR : Muscle type-specific response of HSP60, HSP72, and HSC73 during recovery after elevation of muscle temperature. J. Appl. Physiol. 92, 10971103 (2002)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 15.

    Scelsi R , Marchetti C , Poggi P : Histochemical and ultrastructural aspects of M. vastus lateralis in sedentary old people (age 65–89 years). Acta Neuropathol. 51, 99105 (1980)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 16.

    Selsby JT , Dodd SL : Heat treatment reduces oxidative stress and protects muscle mass during immobilization. Am. J. Physiol. Regul. Integr. Comp. Physiol. 289, R134R139 (2005)

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 17.

    Yoshida N , Morimoto Y , Kataoka H , Sakamoto J , Nakano J , Okita M : Effects of combination therapy of heat stress and muscle contraction exercise induced by neuromuscular electrical stimulation on disuse atrophy in the gastrocnemius. J. Phys. Ther. Sci. 25, 201206 (2013)

    • Crossref
    • Search Google Scholar
    • Export Citation

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