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Shota Inoue
Shota Inoue
Department of Rehabilitation Science, Graduate School of Health Sciences, Kobe University, Kobe , Japan
Research Fellow, Japan Society for the Promotion of Science,Tokyo , Japan
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Research Fellow, Japan Society for the Promotion of Science,
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Kyohei Matsuura
Kyohei Matsuura
Department of Physical Therapy, Faculty of Health Sciences, Kobe University School of Medicine, Kobe , Japan
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Daisuke Eguchi
Daisuke Eguchi
Department of Physical Therapy, Faculty of Health Sciences, Kobe University School of Medicine, Kobe , Japan
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Masahiro Wakayama
Masahiro Wakayama
Department of Physical Therapy, Faculty of Health Sciences, Kobe University School of Medicine, Kobe , Japan
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Kosuke Ono
Kosuke Ono
Department of Physical Therapy, Faculty of Health Sciences, Kobe University School of Medicine, Kobe , Japan
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Hanlin Jiang
Hanlin Jiang
Department of Rehabilitation Science, Graduate School of Health Sciences, Kobe University, Kobe , Japan
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Hideki Moriyama
morihide@harbor.kobe-u.ac.jp
https://orcid.org/0000-0001-7753-0091
Hideki Moriyama
Life and Medical Sciences Area, Health Sciences Discipline, Kobe University, Kobe , Japan
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Abstract
Physical exercise represents one of the most effective approaches to anti-aging. The goal of this study was to verify the effects of different modes and intensities of exercise on longevity proteins in the skeletal muscle in midlife. Middle-aged mice were trained in aerobic or resistance exercise for 8 weeks, and the changes in sirtuin 1 (SIRT1), adenosine monophosphate-activated kinase (AMPK), and mammalian target of rapamycin (mTOR) pathways in the skeletal muscle were evaluated by western blotting. Long-term exercise had no effects on skeletal muscle SIRT1 abundance, whereas high-intensity aerobic exercise increased AMPK phosphorylation and peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α). Low-intensity resistance exercise facilitated Akt/mTOR/p70 ribosomal protein kinase S6 (p70S6K) signaling but did not induce muscle hypertrophy. Conversely, high-intensity resistance exercise stimulated muscle hypertrophy without phosphorylation of mTOR signaling-related proteins. These results suggest the importance of setting exercise modes and intensities for anti-aging in midlife.
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