The study aims to evaluate two-stage anaerobic co-digestion of leachate and starch waste using anaerobic biofilm bioreactor to enhance methane production. The anaerobic digestion process was operated under the mesophilic condition at 35 ± 1 °C. Hydraulic retention time (HRT) applied to the acidogenesis and methanogenesis reactors were 5 and 25 days, respectively. The organic loading rate (OLR) used in the process of acidogenesis was 2.91 gram volatile solid /L.day, while methanogenesis was 0.58 gram volatile solid (VS) per liter per day. Results showed that two-stage process using biofilm was an effective method for operating anaerobic co-digestion of starch waste and landfill leachate in which the system produced higher methane yield at 125.11 mL methane (CH4) per gram volatile solid (VS) added (mL.CH4/g.VS.added) in comparison to the single-stage process (20.57 mL CH4/g.VS.added) and two-stage process (77.60 mL CH4/g.VS.added) without using biofilm. Two-stage process using biofilm also effectively reduced organic matters in the culture in which the system reached 61% BOD removal in comparison to the single-stage process and two-stage process without biofilm that only had 27.6 and 39.3% BOD removal, respectively. This study suggested that the two-stage process using biofilm would be the preferred technique for treating starch waste and landfill leachate.
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