View More View Less
  • 1 College of Pharmacy, King Saud University Department of Pharmacognosy P. O. Box 2457 Riyadh 11451 Saudi Arabia
Restricted access

The hepatoprotective and antioxidant effect of broccoli “Brassica oleracea L.” (aqueous extract of freeze dried powder) on liver injury induced by carbon tetrachloride (CCl 4 ) was investigated. Wistar albino rats weighing 180–200 g were administered a single dose of CCl 4 , GOT, GPT, ALP, GGT and bilirubin were estimated in serum. Non-protein sulfhydryl (NP-SH) and malondialdehyde (MDA) were also estimated in liver tissue. CCl 4 induced hepatotoxicity in all treated animals was evident by elevated serum GOT, GPT, ALP, GGT and bilirubin levels and simultaneous fall in NP-SH and increased MDA activities in liver tissue. The broccoli extract at the doses of 150 and 300 mg/kg produced significant hepatoprotection by decreasing the activity of serum enzymes, bilirubin, while it significantly increased the levels of NP-SH and decreased MDA of liver tissue. Histopathological observations of the liver also showed the protective effect of broccoli in CCl 4 -induced hepatic injury in rats. The obtained results suggest that broccoli possesses hepatoprotective capacity and may have potential therapeutic value in the treatment of some liver disorders probably by its antioxidative effects on hepatocytes, due to flavonoids and sulfurated compounds.

  • Pradeep, K., Mohan, C. V. R., Gobianand, K. et al.: Effect of Cassia fistula Linn. leaf extract on diethylnitrosamine induced hepatic injury in rats. Chemico-Biol. Interact., 2007, 167 , 12–18.

    Gobianand K. , 'Effect of Cassia fistula Linn. leaf extract on diethylnitrosamine induced hepatic injury in rats ' (2007 ) 167 Chemico-Biol. Interact. : 12 -18.

    • Search Google Scholar
  • Nevin, K. G., Vijayammal, P. L.: Effect of Aerva lanata against hepatotoxicity of carbon tetrachloride in rats. Environ. Toxicol. Pharmacol., 2005, 20 , 471–477.

    Vijayammal P. L. , 'Effect of Aerva lanata against hepatotoxicity of carbon tetrachloride in rats ' (2005 ) 20 Environ. Toxicol. Pharmacol. : 471 -477.

    • Search Google Scholar
  • Cho, E. J., Yokozawa, T., Rhyu, D. Y. et al.: The inhibitory effects of 12 medicinal plants and other component compounds on lipid peroxidation. Am. J. Chin. Med., 2003, 31 , 907.

    Rhyu D. Y. , 'The inhibitory effects of 12 medicinal plants and other component compounds on lipid peroxidation ' (2003 ) 31 Am. J. Chin. Med. : 907 -.

    • Search Google Scholar
  • Murugesh, K. S., Yeligar, V. C., Maiti, B. C. et al.: Hepatoprotective and antioxidant role of Berberis tinctoria Lesch. leaves on paracetamol induced hepatic damage in rats. Iranian J. Pharmacol. Therap., 2005, 4 , 64–69.

    Maiti B. C. , 'Hepatoprotective and antioxidant role of Berberis tinctoria Lesch. leaves on paracetamol induced hepatic damage in rats ' (2005 ) 4 Iranian J. Pharmacol. Therap. : 64 -69.

    • Search Google Scholar
  • Singh, J., Upadhyay, A. K., Prasad, K. et al.: Variability of carotenes, vitamin C, E and phenolics in Brassica vegetables. J. Food Compos. Anal., 2007, 20 , 106–112.

    Prasad K. , 'Variability of carotenes, vitamin C, E and phenolics in Brassica vegetables ' (2007 ) 20 J. Food Compos. Anal. : 106 -112.

    • Search Google Scholar
  • Chopra, R. N., Nayar, S. L., Chopra, I. C.: Glossary of Indian medicinal plants. Council of Scientific and Industrial Research, New Delhi, 1956.

    Chopra I. C. , '', in Glossary of Indian medicinal plants , (1956 ) -.

  • Shapiro, T. A., Fahey, J. W., Wade, K. L. et al.: Chemopreventive glucosinolates and isothiocyanates of Broccoli sprouts. Cancer Epidemiol. Biomark. Preven., 2001, 10 , 501–508.

    Wade K. L. , 'Chemopreventive glucosinolates and isothiocyanates of Broccoli sprouts ' (2001 ) 10 Cancer Epidemiol. Biomark. Preven. : 501 -508.

    • Search Google Scholar
  • Vang, O., Frandsen, H., Hansen, K. T. et al.: Biochemical effects of dietary intake of different Broccoli samples. I. Differential modulation of cytochrome P-450 activities in rat liver, kidney and colon. Metabol., 2001, 50 , 1123–1129.

    Hansen K. T. , 'Biochemical effects of dietary intake of different Broccoli samples. I. Differential modulation of cytochrome P-450 activities in rat liver, kidney and colon ' (2001 ) 50 Metabol. : 1123 -1129.

    • Search Google Scholar
  • Kim, P. X. L., Kim, Y. H., Yokozawa, T. et al.: Protective effect of broccoli (Brassica oleracea) and its active components against radical-induced oxidative damage. J. Nutr. Sci. Vitaminol. (Tokyo), 2005, 51 , 142–147.

    Yokozawa T. , 'Protective effect of broccoli (Brassica oleracea) and its active components against radical-induced oxidative damage ' (2005 ) 51 J. Nutr. Sci. Vitaminol. (Tokyo) : 142 -147.

    • Search Google Scholar
  • Fahey, J. W., Haristory, X., Dolan, P. M. et al.: Sulforaphane inhibits extracellular, intracellular, and antibiotic-resistant strains of Helicobacter pylori and prevents benzo[a]pyrene-induced stomach tumors. Proc. Natl. Acad. Sci. USA, 2002, 99 , 7610–7615.

    Dolan P. M. , 'Sulforaphane inhibits extracellular, intracellular, and antibiotic-resistant strains of Helicobacter pylori and prevents benzo[a]pyrene-induced stomach tumors ' (2002 ) 99 Proc. Natl. Acad. Sci. USA : 7610 -7615.

    • Search Google Scholar
  • Farnsworth, N. R.: Biological and phytochemical screening of plants. J. Pharm. Sci., 1966, 55 , 225.

    Farnsworth N. R. , 'Biological and phytochemical screening of plants ' (1966 ) 55 J. Pharm. Sci. : 225 -.

    • Search Google Scholar
  • Rafatullah, S., Mossa, J. S., Ageel, A. M. et al.: Hepatoprotective and safety evaluation studies on sarsaparilla. Int. J. Pharmacog., 1991, 29 , 296–301.

    Ageel A. M. , 'Hepatoprotective and safety evaluation studies on sarsaparilla ' (1991 ) 29 Int. J. Pharmacog. : 296 -301.

    • Search Google Scholar
  • Sedlak, J. and R. H. Lindsay: Estimation of total protein bound and nonprotein sulfhydryl group in tissue with Ellman’s reagents. Analyst. Biochem., 1968, 25 , 192–205.

    Lindsay R. H. , 'Estimation of total protein bound and nonprotein sulfhydryl group in tissue with Ellman’s reagents ' (1968 ) 25 Analyst. Biochem. : 192 -205.

    • Search Google Scholar
  • Utley, H. G., Bernheim, F. and Hochstein, P.: Effect of sulfhydryl reagents on peroxidation in microsomes. Arch. Biochem. Biophys., 1967, 118 , 29–32.

    Hochstein P. , 'Effect of sulfhydryl reagents on peroxidation in microsomes ' (1967 ) 118 Arch. Biochem. Biophys. : 29 -32.

    • Search Google Scholar
  • Dandiya, P. C. and H. Collumbine: Studies on Acorus calamus : Pharmacological actions of essential oil. J. Pharm. Exp. Ther., 1959, 125 , 353.

    Collumbine H. , 'Studies on Acorus calamus: Pharmacological actions of essential oil ' (1959 ) 125 J. Pharm. Exp. Ther. : 353 -.

    • Search Google Scholar
  • Culling, C. F. A.: Handbook of Histopathological and Histochemical Techniques, 3rd Ed. Butterworth and Co., London, 1974, pp. 73, 126 and 159.

    Culling C. F. A. , '', in Handbook of Histopathological and Histochemical Techniques , (1974 ) -.

  • Azri, S., Mata, H. P., Reid, L. L. et al.: Further examination of the selective toxicity of CCl 4 rat liver slices. Toxicol. Appl. Pharmacol., 1992, 112 , 81–86.

    Reid L. L. , 'Further examination of the selective toxicity of CCl4 rat liver slices ' (1992 ) 112 Toxicol. Appl. Pharmacol. : 81 -86.

    • Search Google Scholar
  • Sallie, R., Tredger, J. M., Williams, R.: Drugs and the liver. Biopharm. Drug Dispos., 1991, 12 , 251–259.

    Williams R. , 'Drugs and the liver ' (1991 ) 12 Biopharm. Drug Dispos. : 251 -259.

  • Gupta, M., Mazumdar, U. I., Kumar, T. S. et al.: Antioxidant and hepatoprotective effects of Bauhinia racemosa against paracetamol and carbon tetrachloride induced liver damage in rats. Iranian J. Pharmacol. Therapeut., 2004, 3 , 12–20.

    Kumar T. S. , 'Antioxidant and hepatoprotective effects of Bauhinia racemosa against paracetamol and carbon tetrachloride induced liver damage in rats ' (2004 ) 3 Iranian J. Pharmacol. Therapeut. : 12 -20.

    • Search Google Scholar
  • Moss, D. W., Butterworth, P. J.: Enzymology and Medicine. Pitman Medical (P. 139), London, 1974.

    Butterworth P. J. , '', in Enzymology and Medicine , (1974 ) -.

  • Singh, B., Chandan, B. K., Prabhakar, A.: Chemistry and hepatoprotective activity of an active fraction from Barleria prionitis Linn. in experimental animals. Phytother. Res., 2005, 19 , 391–404.

    Prabhakar A. , 'Chemistry and hepatoprotective activity of an active fraction from Barleria prionitis Linn. in experimental animals ' (2005 ) 19 Phytother. Res. : 391 -404.

    • Search Google Scholar
  • Orhan, D. D., Orhan, N., Ergun, E. et al.: Hepatoprotective effect of Vitis vinifera L. leaves on carbon tetrachloride-induced acute liver damage in rats. J. Ethanopharmacol., 2007, 112 , 145–151.

    Ergun E. , 'Hepatoprotective effect of Vitis vinifera L. leaves on carbon tetrachloride-induced acute liver damage in rats ' (2007 ) 112 J. Ethanopharmacol. : 145 -151.

    • Search Google Scholar
  • Chandan, B. K., Saxena, A. K., Shukla, S. et al.: Hepatoprotective potential of Aloe barbadensis Mill. against carbon tetrachloride induced hepatotoxicity. J. Ethnopharmacol., 2007, 111 , 560–566.

    Shukla S. , 'Hepatoprotective potential of Aloe barbadensis Mill. against carbon tetrachloride induced hepatotoxicity ' (2007 ) 111 J. Ethnopharmacol. : 560 -566.

    • Search Google Scholar
  • Sun, T., Powers, J. R., Tang, J.: Evaluation of the antioxidant activity of asparagus, broccoli and their juices. Food Chem., 2007 (accepted for publication).

  • Brent, J. A., Rumack, B. H.: Role of free radicals in toxic hepatic injury. Clin. Toxicol., 1993, 31 , 173.

    Rumack B. H. , 'Role of free radicals in toxic hepatic injury ' (1993 ) 31 Clin. Toxicol. : 173 -.

    • Search Google Scholar
  • Lim, H. K., Kim, H. S., Choi, H. S. et al.: Hepatoprotective effects of bergenen, a major constituent of Mallotus japonicas on carbon tetrachloride intoxicated rats. J. Ethnopharmacol., 2000, 72 , 469.

    Choi H. S. , 'Hepatoprotective effects of bergenen, a major constituent of Mallotus japonicas on carbon tetrachloride intoxicated rats ' (2000 ) 72 J. Ethnopharmacol. : 469 -.

    • Search Google Scholar
  • Anand, K. K., Singh, B., Saxena, A. K. et al.: 3,4,5-Trihydroxy benzoic acid (gallic acid), the hepatoprotective principle in the fruits of Terminalia blerica – bioassay guided activity. Pharmacol. Res., 1997, 36 , 315–321.

    Saxena A. K. , '3,4,5-Trihydroxy benzoic acid (gallic acid), the hepatoprotective principle in the fruits of Terminalia blerica – bioassay guided activity ' (1997 ) 36 Pharmacol. Res. : 315 -321.

    • Search Google Scholar
  • Sambath, R. K., Sivakumar, T., Sivakumoar, P. et al.: Hepatoprotective and in vivo antioxidant effects of Careya arborea against carbon tetrachloride induced liver damage in rats. Intl. J. Mol. Med. And Adv. Sci., 2005, 1 , 418–424.

    Sivakumoar P. , 'Hepatoprotective and in vivo antioxidant effects of Careya arborea against carbon tetrachloride induced liver damage in rats ' (2005 ) 1 Intl. J. Mol. Med. And Adv. Sci. : 418 -424.

    • Search Google Scholar
  • Fujimoto, J. M., Pearce, K. B., Plaa, G. L.: Barbiturate metabolism as affected by certain agents acting on the liver. J. Pharmacol. Exptl. Ther., 1960, 129 , 139–143.

    Plaa G. L. , 'Barbiturate metabolism as affected by certain agents acting on the liver ' (1960 ) 129 J. Pharmacol. Exptl. Ther. : 139 -143.

    • Search Google Scholar
  • Janbaz, K. H., Saeed, S. A., Gilani, A. H.: Studies on the protective effects of caffeic acid and quercetin on chemical-induced hepatotoxicity in rodents. Phytomedicine, 2007, 11 , 424–427.

    Gilani A. H. , 'Studies on the protective effects of caffeic acid and quercetin on chemical-induced hepatotoxicity in rodents ' (2007 ) 11 Phytomedicine : 424 -427.

    • Search Google Scholar

Hungarian Medical Journal
Language English
Size  
Year of
Foundation
2007
Publication
Programme
ceased
Volumes
per Year
 
Issues
per Year
 
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 1788-6139 (Print)
ISSN 1789-0403 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jun 2021 5 0 0
Jul 2021 13 0 0
Aug 2021 57 0 0
Sep 2021 45 0 0
Oct 2021 3 0 0
Nov 2021 7 0 0
Dec 2021 0 0 0