View More View Less
  • 1 Faculty of Medicine, University of Belgrade Institute of Occupational Medicine and Radiological Protection Deligradska 29 11000 Belgrade Serbia
Restricted access

The observation of chromosome aberration frequency in lymphocyte karyotype is the conclusive method to assess the absorbed dose of ionizing radiation. Our study compared the incidence of chromosome aberrations in occupationally exposed healthy medical workers and in not exposed healthy population. We analyzed the effect of working place, dose by thermo luminescence dosimeter (TLD), duration of occupational exposure (DOE) and age on the sum of aberrant cells and aberrations. The four-year study included 462 subjects with a mean age of 42.3 years, who were occupationally exposed to ionizing radiation and 95 subjects with a mean age of 35.2 years, who were not exposed to ionizing radiation, during the same time period and from the same territory. All of them possess TLD which is read by a scanner for thermo luminescence dosimeters. Modified Moorhead’s micro method for peripheral blood lymphocytes and conventional cytogenetic technique of chromosome aberration analysis were used for the analysis of chromosome aberrations. Stained preparations (Gyms) are observed in immersion by light microscope. The karyotype of 200 lymphocytes in metaphase is analyzed; the most characteristic aberration: dicentric, then the ring and acentric fragments. The incidence of chromosome aberrations was found to be 21.6% in the exposed group and 2.1% in the controls, while the “findings within the limits” (non-specific chromosome lesions – gaps, breaks, elongations, and exchanges) were equal in the two groups (22%). Among occupationally exposed medical workers, the highest incidence was found in nuclear medicine workers (42.6%), then in orthopedists (27.08%). There is a highly significant difference of the number of aberrant cells and the sum of chromosome aberrations between the exposed and control groups ( p < 0.001). The sum of chromosome aberrations and the number of aberrant cells were in positive correlation with the duration of exposure ( p < 0.001), and to a less degree of probability with the age ( p < 0.05) in the exposed workers. In the controls, this correlation was negative and insignificant. The group of subjects with a duration of occupational exposure up to 15 years had a significantly smaller number of aberrant cells and chromosome aberrations in comparison to the subjects with longer duration of occupational exposure, over 15 years.

  • Chu, G.: Double strand break repair. J. Biol. Chem., 1997, 272 , 24097–24100.

    Chu G. , 'Double strand break repair ' (1997 ) 272 J. Biol. Chem. : 24097 -24100.

  • Timbrell, J. A.: Introduction to Toxicology. 3rd ed. London: Taylor – Francis, 2002.

    Timbrell J. A. , '', in Introduction to Toxicology , (2002 ) -.

  • Lodish, H., Berk, A., Zipursky, S. L. et al.: Molecular cell biology. 4th ed. USA: W H Freeman and Company, Media Connected, 2001.

    Zipursky S. L. , '', in Molecular cell biology , (2001 ) -.

  • Natarajan, A. T., Obe, G.: Molecular mechanisms involved in the production of chromosomal aberrations, I. Utilisation of neurospora end nuclease for the study of aberration production in G-2 stages of cell cycle. Mutation Res., 1978, 52 , 137.

    Obe G. , 'Molecular mechanisms involved in the production of chromosomal aberrations, I. Utilisation of neurospora end nuclease for the study of aberration production in G-2 stages of cell cycle ' (1978 ) 52 Mutation Res. : 137 -.

    • Search Google Scholar
  • Natarajan, A. T., Obe, G.: Molecular mechanisms involved in the production of chromosomal aberrations, II. Utilisation of neurospora end nuclease for the study of aberration production by x-rays in G-1 and G-2 stages of cell cycle. Mutation Res., 1980, 69 , 293.

    Obe G. , 'Molecular mechanisms involved in the production of chromosomal aberrations, II. Utilisation of neurospora end nuclease for the study of aberration production by x-rays in G-1 and G-2 stages of cell cycle ' (1980 ) 69 Mutation Res. : 293 -.

    • Search Google Scholar
  • Natarajan, A. T., Waynesburg, T. S. B: Mechanisms for chromosomal aberrations in mammalian cells. Mutation Res., 1982, 95 , 1–2.

    Waynesburg T. S. B , 'Mechanisms for chromosomal aberrations in mammalian cells ' (1982 ) 95 Mutation Res. : 1 -2.

    • Search Google Scholar
  • Pfeiffer, P., Goedecke, W., Obe, G.: Mechanisms of DNA double break repair and their potential to induce chromosomal aberrations. Mutagenesis, 2000, 15 , 289–302.

    Obe G. , 'Mechanisms of DNA double break repair and their potential to induce chromosomal aberrations ' (2000 ) 15 Mutagenesis : 289 -302.

    • Search Google Scholar
  • Goodhead, D. T.: Spatial and temporal distribution of energy. Health Physics, 1988, 55 , 231–40.

    Goodhead D. T. , 'Spatial and temporal distribution of energy ' (1988 ) 55 Health Physics : 231 -40.

    • Search Google Scholar
  • Moorhead, P. S.: Chromosome preparation of leukocytes cultured from human peripheral blood. Exp. Cell Res., 1960, 20 , 613–16.

    Moorhead P. S. , 'Chromosome preparation of leukocytes cultured from human peripheral blood ' (1960 ) 20 Exp. Cell Res. : 613 -16.

    • Search Google Scholar
  • Joksić, G., Nikolić, M., Spasojević-Tišma, V.: Radio sensitivity of different aged human lymphocytes following electron on irradiation in vitro. Neoplasm, 1997, 44 , 117–121.

    Spasojević-Tišma V. , 'Radio sensitivity of different aged human lymphocytes following electron on irradiation in vitro ' (1997 ) 44 Neoplasm : 117 -121.

    • Search Google Scholar
  • Law on Ionizing Radiation Protection, Official Gazette of the Federal Rpublic of Yugoslavia, No. 46/96.

  • Rozgaj, R., Kašuba, V., Šimić, D.: The frequency of dicentrics and acentrics and the incidence of rogue cells in radiation workers. Mutagenesis, 2002, 17 , 135–139.

    Šimić D. , 'The frequency of dicentrics and acentrics and the incidence of rogue cells in radiation workers ' (2002 ) 17 Mutagenesis : 135 -139.

    • Search Google Scholar
  • Bonassi, S.: Chromosomal aberrations in lymphocytes predict human cancer independently of exposure to carcinogens. Cancer Res., 2002, 15 , 1619–1625.

    Bonassi S. , 'Chromosomal aberrations in lymphocytes predict human cancer independently of exposure to carcinogens ' (2002 ) 15 Cancer Res. : 1619 -1625.

    • Search Google Scholar
  • Rozgaj, R., Kašuba, V., Peric, M.: Chromosome aberrations in operating room personnel. Am. J. Ind. Med., 1999, 35 , 642–646.

    Peric M. , 'Chromosome aberrations in operating room personnel ' (1999 ) 35 Am. J. Ind. Med. : 642 -646.

    • Search Google Scholar
  • Senthamizhchelvan, S., Pant, G. S., Rath, G. et al.: Biodosimetry using chromosome aberrations in human lymphocytes. Radiat. Prot. Dosim., 2007, 123 , 241–245.

    Rath G. , 'Biodosimetry using chromosome aberrations in human lymphocytes ' (2007 ) 123 Radiat. Prot. Dosim. : 241 -245.

    • Search Google Scholar
  • Blakely, W. F., Salter, C. A., Prasanna, P.: Early-response biological dosimetry – recommended countermeasure enhancements for mass-casualty radiological incidents and terrorism. Health Phys., 2005, 89 , 494–504.

    Prasanna P. , 'Early-response biological dosimetry – recommended countermeasure enhancements for mass-casualty radiological incidents and terrorism ' (2005 ) 89 Health Phys. : 494 -504.

    • Search Google Scholar
  • Bonassi, S., Norppa, H., Ceppi, M. et al.: Chromosomal aberration frequency in lymphocytes predicts the risk of cancer: Results from a pooled cohort study of 22,358 subjects in 11 countries. Carcinogenesis, 2008 Mar 19 [E-pub ahead of print.

  • Boffetta, P., Hel, O., Norppa, H. et al.: Chromosomal aberrations and cancer risk: results of a cohort study from Central Europe. Am. J. Epidemiol., 2007, 165 , 36–43.

    Norppa H. , 'Chromosomal aberrations and cancer risk: results of a cohort study from Central Europe ' (2007 ) 165 Am. J. Epidemiol. : 36 -43.

    • Search Google Scholar
  • Birchall, A., Puncher, M., James, A. C. et al.: Internal dosimetry made simple. Proceedings of the workshop on internal dosimetry of radionuclides. Radiat. Prot. Dosim., 2003, 105 , 421–425.

    James A. C. , 'Internal dosimetry made simple. Proceedings of the workshop on internal dosimetry of radionuclides ' (2003 ) 105 Radiat. Prot. Dosim. : 421 -425.

    • Search Google Scholar
  • Bolognesi, C., Abbondandolo, A., Barale, R. et al.: Age-related increase of chromosome aberrations, sister-chromatid exchanges and micronuclei in human lymphocytes. Cancer Epidem. Biomar. Prev., 1997, 6 , 249–256.

    Barale R. , 'Age-related increase of chromosome aberrations, sister-chromatid exchanges and micronuclei in human lymphocytes ' (1997 ) 6 Cancer Epidem. Biomar. Prev. : 249 -256.

    • Search Google Scholar
  • Bonassi, S., Ugolini, D., Kirsh-Volders, M. et al.: Human population studies with cytogenetic biomarkers: Review of the literature and future prospective. Environ. Mol. Mutagen., 2005, 45 , 258–270.

    Kirsh-Volders M. , 'Human population studies with cytogenetic biomarkers: Review of the literature and future prospective ' (2005 ) 45 Environ. Mol. Mutagen. : 258 -270.

    • Search Google Scholar
  • ICRP: Individual monitoring for internal exposure of workers. ICRP Report, 1997, 78 , 47–52.

    'Individual monitoring for internal exposure of workers ' (1997 ) 78 ICRP Report : 47 -52.

  • Günter, S., Oestreicher, U., Romm, H.: Biological Dosimetry. In: Günter, O. (Ed.): Chromosomal Alterations. Springer, Berlin-Heidelberg, 2007, pp. 341–350.

    Romm H. , '', in Chromosomal Alterations , (2007 ) -.

  • Wojewodzka, M., Kruszewski, M., Iwaneriko, T. et al.: Application of the comet assay for monitoring DNA damage in workers exposed to chronic low-dose irradiation. Mutat. Res./Genetic Toxicology and Environmental Mutagenesis, 1998, 416 , 21–35.

    Iwaneriko T. , 'Application of the comet assay for monitoring DNA damage in workers exposed to chronic low-dose irradiation ' (1998 ) 416 Mutat. Res./Genetic Toxicology and Environmental Mutagenesis : 21 -35.

    • Search Google Scholar

Hungarian Medical Journal
Language English
Size  
Year of
Foundation
2007
Publication
Programme
ceased
Volumes
per Year
 
Issues
per Year
 
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 1788-6139 (Print)
ISSN 1789-0403 (Online)