Hungarian Medical Journal
Volume/Issue:
Volume 2: Issue 3
A Case of IgG kappa-LD Complex, Producing Long-Term Increase of Enzyme Activity and a Rare Isoenzyme Pattern
Authors:
L. Góth Medical and Health Science Center, University of Debrecen Department of Clinical Biochemistry and Molecular Pathology and Clinical Analytical Chemistry PO Box 55 H-4012 Debrecen Hungary

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W. N. Bigler San Francisco State University Center for Biomedical Laboratory Science San Francisco CA USA

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, and
E. Szomják Medical and Health Science Center, University of Debrecen 3rd Department of Internal Medicine Debrecen Hungary

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Pages:
443–449
Online Publication Date:
28 Sep 2008
Publication Date:
01 Sep 2008
Article Category:
Research Article
DOI:
https://doi.org/10.1556/hmj.2.2008.3.11
Keywords:
elevated LD; LD-IgG; macro-LD; atherosclerosis; stent
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Background: Serum LD activity is increased in several diseases. The clinical significance of elevated LD may be evaluated by isoenzyme analysis. Here, we report on a long-term elevation of serum LD activity with a rare isoenzyme pattern caused by LD-IgG complexes. Patient, methods: A 75-year old female with atherosclerosis and implantations of two stents showed increased LD activity and an unusual isoenzyme pattern. LD activity, inhibition and immunoglobulins were measured with Roche tests; immunofixation was performed with a Helena test. LD isoenzymes were separated in agarose gel and quantified by the color formed by NADH, reduced phenazine methosulfate and nitro blue tetrazolium. Results: LD isoenzyme analyses revealed one band near the slowly migrating LD-4 position. The band contained LD complexed with immunoglobulins (IgG with κ light chains), The long-term increased serum LD enzyme activity in this patient may have been due to abnormally slow clearance of these large macro-LD complexes from circulation. Conclusions: An increased LD activity and a rare LD-IgG pattern were detected for five years in a patient who had not rare diseases but atherosclerosis and two stents. Evaluation of LD activity and its isoenzyme test results may be complicated and misleading for patients with the same LD-IgG complex in their serum.

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Cover Hungarian Medical Journal
Hungarian Medical Journal
Print ISSN:
1788-6139
Online ISSN:
1789-0403

Hungarian Medical Journal
Language English
Size  
Year of
Foundation		 2007
Publication
Programme		 ceased
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per Year		  
Issues
per Year		  
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
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Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 1788-6139 (Print)
ISSN 1789-0403 (Online)

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