Hungarian Medical Journal
Volume/Issue:
Volume 2: Issue 3
mRNA Expression Analysis and Classification of Colonic Biopsy Samples Using Oligonucleotide and cDNA Microarray Techniques
Author:
Orsolya Galamb Semmelweis University 2nd Department of Medicine Szentkirályi u. 46, H-1088 Budapest Hungary

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Pages:
467–474
Online Publication Date:
28 Sep 2008
Publication Date:
01 Sep 2008
Article Category:
Research Article
DOI:
https://doi.org/10.1556/hmj.2.2008.28397
Keywords:
gene expression signature; microarray; colorectal cancer; inflammatory bowel diseases; adenoma
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Despite tremendous progress in the past few decades, certain important aspects regarding the diagnosis, therapy, and follow-up of colorectal cancer still remain unsolved. In our study we searched for biomarkers of the development of colorectal carcinoma, and performed gene expression analysis for colorectal disease classification. We have established that the oligonucleotide microarray analyses of biopsy samples wholly fulfill the Affymetrix quality requirements, are highly standard and reproducible and the Taqman Microfluidic Card System is suitable for high-throughput, quick and cost efficient real-time-PCR validation of gene expression changes. We have shown that the sequential overexpression of osteopontin and osteonectin mRNAs and proteins significantly correlates with the progression of the colorectal adenoma-dysplasia-carcinoma sequence. We have identified and validated ten novel markers with continuously increasing mRNA expression in line with the adenoma-dysplasia-carcinoma transition. We have identified the top 27, 13 and 10 genes associated with adenoma, colorectal cancer, and inflammatory bowel diseases.

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Cover Hungarian Medical Journal
Hungarian Medical Journal
Print ISSN:
1788-6139
Online ISSN:
1789-0403

Hungarian Medical Journal
Language English
Size  
Year of
Foundation		 2007
Publication
Programme		 ceased
Volumes
per Year		  
Issues
per Year		  
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 1788-6139 (Print)
ISSN 1789-0403 (Online)

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