Despite tremendous progress in the past few decades, certain important aspects regarding the diagnosis, therapy, and follow-up of colorectal cancer still remain unsolved. In our study we searched for biomarkers of the development of colorectal carcinoma, and performed gene expression analysis for colorectal disease classification. We have established that the oligonucleotide microarray analyses of biopsy samples wholly fulfill the Affymetrix quality requirements, are highly standard and reproducible and the Taqman Microfluidic Card System is suitable for high-throughput, quick and cost efficient real-time-PCR validation of gene expression changes. We have shown that the sequential overexpression of osteopontin and osteonectin mRNAs and proteins significantly correlates with the progression of the colorectal adenoma-dysplasia-carcinoma sequence. We have identified and validated ten novel markers with continuously increasing mRNA expression in line with the adenoma-dysplasia-carcinoma transition. We have identified the top 27, 13 and 10 genes associated with adenoma, colorectal cancer, and inflammatory bowel diseases.
Cancer Research Campaign: Cancer statistics: Large bowel – UK. London: CRC. 1999.
'', in Cancer statistics: Large bowel , (1999 ) -.
Szentirmay, Z., Csuka, O.: Molecular pathology of colorectal cancer. In Tulassay, Z. (ed.): Prevention and treatment of colorectal cancer (pp. 31–62). Budapest, Springer Scientific Publisher, 2004.
Csuka O. , '', in Prevention and treatment of colorectal cancer , (2004 ) -.
O’Connell, J. B., Maggard, M. A., Ko, C. Y.: Colon cancer survival rates with the new American Joint Committee on Cancer sixth edition staging. J. Nat. Cancer Inst., 2004, 96 , 1420–1425.
Ko C. Y. , 'Colon cancer survival rates with the new American Joint Committee on Cancer sixth edition staging ' (2004 ) 96 J. Nat. Cancer Inst. : 1420 -1425 .
Herszenyi, L., Sipos, F., Galamb, O. et al.: Matrix metalloproteinase-9 expression in the normal mucosa-adenoma-dysplasia-adenocarcinoma sequence of the colon. Pathol. Oncol. Res., 2008 (in press).
Sipos, F., Galamb, O., Herszenyi, L. et al.: Elevated insulin-like growth factor 1 receptor, hepatocyte growth factor receptor and telomerase protein expression in mildly active ulcerative colitis. Scand. J. Gastroenterol., 2008, 43 , 289–298.
Herszenyi L. , 'Elevated insulin-like growth factor 1 receptor, hepatocyte growth factor receptor and telomerase protein expression in mildly active ulcerative colitis ' (2008 ) 43 Scand. J. Gastroenterol. : 289 -298 .
Hagymasi, K., Molnar, B., Sipos, F. et al.: Stem cell theory of colorectal cancer and its connection with molecular-biological data. Orv. Hetil., 2007, 148 , 779–785.
Sipos F. , 'Stem cell theory of colorectal cancer and its connection with molecular-biological data ' (2007 ) 148 Orv. Hetil. : 779 -785 .
Model, F., Osborn, N., Ahlquist, D. et al.: Identification and validation of colorectal neoplasia-specific methylation markers for accurate classification of disease. Molec. Cancer Res., 2007, 5 , 153–163.
Ahlquist D. , 'Identification and validation of colorectal neoplasia-specific methylation markers for accurate classification of disease ' (2007 ) 5 Molec. Cancer Res. : 153 -163 .
Miheller, P., Muzes, G., Galamb, O. et al.: Molecular biology background of inflammatory bowel disease. Orv. Hetil., 2006, 147 , 1395–1403.
Galamb O. , 'Molecular biology background of inflammatory bowel disease ' (2006 ) 147 Orv. Hetil. : 1395 -1403 .
Molnar, B., Sipos, F., Galamb, O. et al.: Molecular detection of circulating cancer cells. Dig. Dis., 2003, 21 , 320–325.
Galamb O. , 'Molecular detection of circulating cancer cells ' (2003 ) 21 Dig. Dis. : 320 -325 .
Galamb, O., Gyorffy, B., Sipos, F. et al.: Inflammation, adenoma and cancer: objective classification of colon biopsy specimens with gene expression signature. Dis. Markers, 2008 (in press).
Galamb, O., Gyorffy, B., Sipos, F. et al.: Helicobacter pylori and antrum erosion specific gene expression patterns: the discriminative role of CXCL13 and VCAM1 transcripts. Helicobacter, 2008, 13 , 112–126.
Sipos F. , 'Helicobacter pylori and antrum erosion specific gene expression patterns: the discriminative role of CXCL13 and VCAM1 transcripts ' (2008 ) 13 Helicobacter : 112 -126 .
Galamb, O., Sipos, F., Dinya, E. et al.: Functional mRNA expression analysis and classification of colonic biopsy samples using overall cDNA microarray technique. Orv. Hetil., 2008, 149 , 219–232.
Dinya E. , 'Functional mRNA expression analysis and classification of colonic biopsy samples using overall cDNA microarray technique ' (2008 ) 149 Orv. Hetil. : 219 -232 .
Galamb, O., Gyorffy, B., Sipos, F. et al.: Identification of colorectal cancer, adenoma, and inflammatory bowel disease specific gene expression patterns using whole genomic oligonucleotide microarray system. Orv. Hetil., 2007, 148 , 2067–2079.
Sipos F. , 'Identification of colorectal cancer, adenoma, and inflammatory bowel disease specific gene expression patterns using whole genomic oligonucleotide microarray system ' (2007 ) 148 Orv. Hetil. : 2067 -2079 .
Galamb, O., Sipos, F., Molnar, B. et al.: Evaluation of malignant and benign gastric biopsy specimens by mRNA expression profile and multivariate statistical methods. Cytometry B Clin. Cytometry, 2007, 72 , 299–309.
Molnar B. , 'Evaluation of malignant and benign gastric biopsy specimens by mRNA expression profile and multivariate statistical methods ' (2007 ) 72 Cytometry B Clin. Cytometry : 299 -309 .
Galamb, O., Sipos, F., Dinya, E. et al.: mRNA expression, functional profiling and multivariate classification of colon biopsy specimen by cDNA overall glass microarray. World J. Gastroenterol., 2006, 12 , 6998–7006.
Dinya E. , 'mRNA expression, functional profiling and multivariate classification of colon biopsy specimen by cDNA overall glass microarray ' (2006 ) 12 World J. Gastroenterol. : 6998 -7006 .
Galamb, O., Sipos, F., Fischer, K. et al.: The results of the expression array studies correlate and enhance the known genetic basis of gastric and colorectal cancer. Cytometry B Clin. Cytometry, 2005, 68 , 1–17.
Fischer K. , 'The results of the expression array studies correlate and enhance the known genetic basis of gastric and colorectal cancer ' (2005 ) 68 Cytometry B Clin. Cytometry : 1 -17 .
Galamb, O., Molnar, B., Tulassay, Z.: DNA chips for gene expression analysis and their application in diagnostics. Orv. Hetil., 2003, 144 , 21–27.
Tulassay Z. , 'DNA chips for gene expression analysis and their application in diagnostics ' (2003 ) 144 Orv. Hetil. : 21 -27 .
Toth, K., Galamb, O., Hever-Palfy, T. et al.: Peripheral blood gene expression markers of colorectal diseases. Magy. Belorv. Arch., 2007, 60 , 531–539.
Hever-Palfy T. , 'Peripheral blood gene expression markers of colorectal diseases ' (2007 ) 60 Magy. Belorv. Arch. : 531 -539 .
Nagy, G. R., Gyorffy, B., Galamb, O. et al.: Use of routinely collected amniotic fluid for whole-genome expression analysis of polygenic disorders. Clin. Chem., 2006, 52 , 2013–2020.
Galamb O. , 'Use of routinely collected amniotic fluid for whole-genome expression analysis of polygenic disorders ' (2006 ) 52 Clin. Chem. : 2013 -2020 .
Sipos, F., Galamb, O., Molnar, B. et al.: Use of DNA-chips technology in colorectal cancer. Orv. Hetil., 2004, 145 , 993–999.
Molnar B. , 'Use of DNA-chips technology in colorectal cancer ' (2004 ) 145 Orv. Hetil. : 993 -999 .
Spisak, S., Molnar, B., Galamb, O. et al.: Theoretical foundations of protein chips and their possible use in medical research and diagnostics. Orv. Hetil., 2007, 148 , 1511–1520.
Galamb O. , 'Theoretical foundations of protein chips and their possible use in medical research and diagnostics ' (2007 ) 148 Orv. Hetil. : 1511 -1520 .
Tumor Analysis Best Practices Working Group: Expression profiling-best practices for data generation and interpretation in clinical trials. Nature Rev. Genet., 2004, 5 , 229–237.
'Expression profiling-best practices for data generation and interpretation in clinical trials ' (2004 ) 5 Nature Rev. Genet. : 229 -237 .
Kendall, M. G.: Rank Correlation Methods. 4th Ed. Griffin. 1976.
Ladanyi, A., Sipos, F., Szoke, D. et al.: Laser microdissection in translational and clinical research. Cytometry A, 2006, 69 , 947–60.
Szoke D. , 'Laser microdissection in translational and clinical research ' (2006 ) 69 Cytometry A : 947 -60 .
Sipos, F., Szoke, D., Galamb, O. et al.: The importance and use of laser microdissection in clinical research and diagnostic method development. Orv. Hetil., 2004, 145 , 1355–1361.
Galamb O. , 'The importance and use of laser microdissection in clinical research and diagnostic method development ' (2004 ) 145 Orv. Hetil. : 1355 -1361 .