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Szarvasmarhatartó telepen alkalmazott ivarzásindukáló hormonok megjelenése a hígtrágyában
Appearance of on-farm bovine reproductive hormones in the resulting slurry
A nemzetközi irodalmat is áttekintve azt találtuk, hogy az intenzív tejelő szarvasmarhatartásban felhasznált ivarzásindukáló hormonkészítmények mennyiségét és a hígtrágyában való megjelenését még nem vizsgálták. Kutatásunkban egy Pest megyei szarvasmarhatelepen használt 5 különböző ivarzásindukáló gyógyszer (Alfaglandin, PGF, Dinolytic, Gonavet, Ovarelin) és ezen belül 3 hatóanyag (D-Phe6-gonadorelin, kloprosztenol és dinoproszt-trometamin) sorsát követtük nyomon a felhasználástól egészen a hígtrágyában való megjelenéséig, 2017-től 2020-ig. A tanulmány során áttekintettük a gyógyszerfogyást, valamint minden évben negyedéves ciklusokban, évszakonként vizsgáltuk meg a telepen keletkezett hígtrágya hormonhatását. Külön teszteltük a telepen alkalmazott hormonkészítmények hormonhatását is. Az ösztrogénhatás vizsgálatokhoz a humán ösztrogénreceptort tartalmazó élesztőtesztet alkalmaztuk az ISO 19040 szabvány alapján. Az eredmények statisztikai értékelésével (Pearson-féle korreláció és főkomponens-elemzés) az ivarzásindukálók felhasználása, a telep szaporodásbiológiája és a hígtrágya ösztrogénhatása közötti összefüggéseket tártuk fel. Megállapítottuk, hogy a hígtrágya és az iszap ösztrogénhatása erősen összefügg. Mindhárom vizsgált gyógyszerhatóanyag erős korrelációt mutatott a hígtrágya/iszap ösztrogénhatásával. Vizsgálataink alátámasztják, hogy a hígtrágya egy olyan anyag, melyet a szántóföldre történő kijuttatás előtt számos egyéb ok mellett a hormon- és gyógyszertartalma miatt is új kezelési módszerekkel kell ártalmatlanítani, nemcsak környezetegészségügyi szempontból, hanem az egészségügyi kockázatok miatt is, valamint hogy a megfelelő gyógyszerválasztással a hígtrágya hormonhatása redukálható.
Cover crops serve as an essential source of nutrients in the soil and generally improve the soil’s properties. Cover crops’ production is considered a benefit of the soil quality; by protecting the soil from erosion, reducing the weeds and the so-called soil-borne plant pathogens. Different varieties of cover crops can be cultivated such as legumes, non-legumes, brassica, and grass-type of plants with a variability of the symbiosis. A pot experiment was carried out with five cover crops, as non-symbiont (Brassica carinata B.c.), single-symbiont with arbuscular mycorrhiza fungi (AMF) (Phacelia tanacetifolia P.t., Avena strigosa A.s.) and double symbiont with AMF and nitrogen-fixing bacteria (Vicia benghalensis V.b., Vicia faba V.f.) crops; and a mixture of the five species, placed in sandy soil (arenosol) in plastic pots (5000 g soil) in 4 repetitions. One of the pots with mixed cover crops was inoculated by AM fungi industrial product. We measured soil biological activity of dehydrogenase (DHA) and fluorescein-diacetate (FDA) enzymes, the frequency of AM fungi (F%), the all protein, glomalin content and electrical conductivity (EC) of the soils. Mixture of all the cover crops resulted maximum EC and significantly enhanced the enzymatic, DHA, FDA activities in comparison with single plants. Mycorrhiza colonization frequency was high in all cover crops except the mustard (B.c.), as nonsymbiont. Vetch (V.b.), as double symbiont was responding very positively to AMF inoculation, and enhanced the performance of its growth. It was found in the pot experiment, that vetch, has the highest capacity to retain soil-protein, glomalin concentration, as well. The mixture of five cover crops could be suggested to use, due to the synergistic positive performance of the individual crops, and the better functioning of beneficial fungal / bacterial symbiosis.
Open-field small plot long-term experiment was set up during 2011 with willow (Salix triandra × S. viminalis ‘Inger’), grown as a short rotation coppice energy crop in Nyíregyháza, Hungary. The sandy loam Cambisol with neutral pH was treated three times (2011, 2013, and 2016) with 15 t ha–1 municipal sewage sludge compost (MSSC) and with 600 kg ha–1 (2011, 2013) or 300 kg ha–1 (2016) wood ash (WA). In 2018 the MSSC-treated plots were amended with 7.5 t ha–1 municipal sewage sediment (MSS), and 300 kg ha–1 WA. MSSC and WA or MSS and WA were also applied to the soil in combinations during all treatments. Control plots remained untreated since 2011. Repeated application of wastewater solids (MSSC, MSS) and wood ash (WA) significantly enhanced the amounts of As (up to +287%), Ba, Cd (up to +192%), Cu, Mn, Pb, and Zn in the topsoil of willows. The combined application of MSSC+MSS+WA resulted in significantly higher Mn and Zn and lower As Ba, Cd Cr, and Pb concentrations in topsoil than MSSC+MSS treatment of soil without WA. Nitrogen concentrations in leaves of treated plants were generally slightly lower or similar to control. All soil treatments significantly enhanced the uptake or accumulation of nutrient elements (Ca, K, Mg, P) and potentially toxic elements (As, Ba, Cd, Cr, Cu, Mn, Ni, Pb, and Zn) in the leaves of willows during 2018, 2019, and 2020. Significantly higher Mn or Zn concentrations were measured in MSSC+MSS+WA than in MSSC+MSS treatments. Significant amounts of Cd (up to 1.11 mg kg–1) or Zn (up to 183 mg kg–1) can be translocated (phytoextracted) from a soil amended with wastewater solids or wood ash to willow leaves. In 2018 the treatments decreased the chlorophyll fluorescence values, while in 2019 and 2020 the light adapted fluorescence yield (Y) values were higher in treated than in control plants.
In this study PTEs, [potentially toxic elements (Cr, Cu, Mn, Ni, Pb, and Zn)] were investigated in the upper layer of floodplain soils that occurred as a result of accident in the area of two mine tailings in Northwestern Romania. A large amount of sediment was deposited on the soil of floodplains along the Hungarian section of River Tisza, which could represent a threat to the environment. Floodplain soil samples were collected from four locations in Hungary from an area of the river stretching to about 250 km. BCR (Bureau Communautaire de Référence) sequential extraction method was used to analyze both post-flood and present samples. Most of the analyzed elements (Cd, Cr, Cu, Ni, Pb, Zn) were found in the residual fraction, but there is a notable soluble amount in hydroxylammonium chloride extractable fraction. The results allow a comparison of the changes that have taken place over time, in addition to serving as a basis for further studies.
Due to extreme meteorological and soil hydrological situations the agricultural production security is highly unpredictable. To release the extent and duration of inland excess water (IEW) inundations or two-phase soil conditions during the period intended for cultivation, subsurface drainage (SD) has been used as a best practice in several countries. SD interventions took place between 1960’s and 1990 in Hungary. After 1989, land ownership conditions changed, thus professional operation and the necessary maintenance of the SD networks designed as a complex system became insignificant. In this paper, our aim was to present the IEW hazard in one of the most equipped areas by SD in Hungary. The occurrence frequency of IEW inundations in drained and non-drained (control) areas in different time intervals were compared. According to our results, we could state that the frequency of IEW on the subsurface drained areas was moderately lower in only a few periods compared to the control areas. IEW hazard of the arable areas at the Körös Interfluve was classified as nonhazarded in 52.7% of the area. Another 38.2% were moderately hazarded, 8.26% of the lands were meanly hazarded and less than 1% were highly hazarded area by IEW.
The Westsik’s long-term crop rotation experiment was set up in 1929 at the Nyíregyháza Experimental Station (NE Hungary) on a slightly acidic Arenosol. Besides fallow crop rotation (CR), effects of different organic amendments (lupine as green manure, lupine as main crop, straw manure, and farmyard manure (FYM) were studied with or without N or NPK-fertilizers. The crop rotation consisted of rye, potato, lupine, and oat with common vetch. The soil of potato plots was analysed in 2019 at the 90th anniversary of Westsik’s crop rotation experiment.
The following chemical and microbiological soil parameters were determined: soil pH, available nutrient contents, organic carbon (OC) and nitrogen (ON) contents, microbial biomass carbon (MBC) and nitrogen (MBN), soil respiration, net nitrification, and activity of some soil enzymes.
In the CRs, the soil pHH2O varied from acidic to weakly alkaline and it largely differed from pHKCl. The results showed a significant increase in the content of nitrate, available phosphorus and potassium in most of the fertilized plots. Applying straw, green manure, or FYM significantly increased the OC and ON contents. The total count of cultivable bacteria increased upon the application of the organic manures. Combined application of straw manure and N-fertilization heavily improved the abundance of the microscopic fungi.
While all the applied organic manures significantly enhanced the MBC, the MBN increased only by the green manure amendment. Our results revealed higher soil respiration rate in the plots receiving straw or FYM than in the control. Both green manure and FYM elevated the net nitrification rate. Phosphatase, saccharase, urease, and dehydrogenase enzymes showed a hesitating response to the manure application in the different CRs.
The soil respiration and dehydrogenase activity correlated to most of the measured chemical parameters. Among microbiological properties, the MBC and MBN, as well as dehydrogenase and other enzyme activities displayed a positive correlation. Results proved the need for the exogenous application of organic matter in the form of organic manures to enhance the nutritional status and health of the soil.
Greenhouse plastic contaminations in agricultural soils were studied to quantify and examine the macroplastic and microplastic contaminants on the soil surface, soil profile, and groundwater under greenhouse farmland. Random sampling was used to select three areas in a greenhouse farm where macroplastic and microplastic data were collected. Four composite samples were collected from shallow (0–20 cm) and deep (20–40 cm) soils for each sampling point, respectively. Three soil profiles were dug, and samples were collected at intervals of 20 cm. Groundwater samples were also collected from the same profiles at a depth of 100 cm. Microplastics were extracted using predigestion of organic matter with 30% H2O2 and density separation with ZnCl2. The total mass of macroplastics in the greenhouse farmland was 6.4 kg ha–1. Polyethylene and polyvinyl chloride were the dominant plastic structures, and the dominant sizes were 1–5 and 0.5–1.0 cm, respectively. Overall, the average abundance of microplastics in the greenhouse soil was 225 ± 61.69 pieces/kg, and the dominant size structure was 2–3 mm. The average microplastic concentrations at depths of 0–20 and 20–40 cm were 300 ± 93 and 150.0 ± 76.3 pieces/kg, respectively. The average microplastic concentration in the groundwater was 2.3 pieces/l, and fibers were the dominant plastic structure. Given that microplastics were found in greenhouse soil, soil profiles, and groundwater, we recommend the careful cleaning and disposal of plastics on greenhouse farmland and further research to shed light on the level of microplastic contamination in the soil profiles and groundwater.
Abstract
We have investigated the deformation history of the Keszthely Hills (Transdanubian Range, W Hungary), which belongs to the uppermost slice of the Austroalpine nappe system. This Upper Triassic to Upper Miocene sedimentary rock sequence documented the deformation of the upper crust during repeated rifting and inversion events. We investigated the structural pattern and stress field evolution of this multistage deformation history by structural data collection and evaluation from surface outcrops. Regarding the Mesozoic deformations, we present additional arguments for pre-orogenic (Triassic and Jurassic) extension (D1 and D2 phases), which is mainly characterized by NE–SW extensional structures, such as syn-sedimentary faults, slump-folds, and pre-tilt conjugate normal fault pairs. NW–SE-striking map-scale normal faults were also connected to these phases.
The inversion of these pre-orogenic structures took place during the middle part of the Cretaceous; however, minor contractional deformation possibly reoccurred until the Early Miocene (D3 to D5 phases). The related meso- and map-scale structures are gentle to open folds, thrusts and strike-slip faults. We measured various orientations, which were classified into three stress states or fields on the basis of structural criteria, such as tilt-test, and/or superimposed striae on the same fault planes. For this multi-directional shortening we presented three different scenarios. Our preferred suggestion would be the oblique inversion of pre-orogenic faults, which highly influenced the orientation of compressional structures, and resulted in an inhomogeneous stress field with local stress states in the vicinity of inherited older structures.
The measured post-orogenic extensional structures are related to a new extensional event, the opening of the Pannonian Basin during the Miocene. We classified these structures into the following groups: immediate pre-rift phase with NE–SW extension (D6), syn-rift phase with E–W extension (D7a) and N–S transpression (D7b), and post-rift phase with NNW–SSE extension (D8).
Abstract
Over the almost 190 years-long research of the Ditrău Alkaline Massif (Eastern Carpathians, Romania), felsic rocks have been regarded as homogeneous, uniform units of the igneous complex. Nevertheless, our detailed textural study revealed that the felsic suite (diorite–alkaline feldspar syenite and nepheline-bearing syenite–granite series) cropping out north of the Jolotca Creek valley is more heterogenous at micro-scale than previously thought. This heterogeneity partly derives from abundant mafic mineral-rich clusters; nevertheless, felsic minerals also exhibit various, remarkable textural features. Outcrop to micro-scale traits of felsic crystal settling, mafic mineral aggregates and flow fabrics along with metamorphic country rock xenoliths suggest that the studied rocks crystallized under dynamic magmatic conditions. Cumulate formation, shear flow, convection currents as well as various open-system magmatic processes (e.g., magma recharge, magma mixing and mingling, crystal or mush transfer and recycling, country rock assimilation) played a significant role in the petrogenesis of the examined felsic suite.
Based on field observations as well as on the microtextural relationship of the minerals, two major groups of felsic rocks were distinguished: (1) felsic rocks (lacking or containing sparse mafic minerals) spatially associated with mafic rocks and (2) felsic rocks (with mafic minerals and clots) spatially unassociated with mafic rocks. Rocks of the former group are dominated by plagioclase, accompanied by minor alkaline feldspar, biotite and accessory titanite. Distinct structural and textural features suggest the physical accumulation of the rock-forming phases. Such textural properties can also be observed in some rocks of the second group. Isolated mafic minerals are rather scarce in the latter; nevertheless, different types of aggregates made up of either identical or various mafic phases are more common. Clustered minerals are either intact or show different stages of alteration.
A detailed petrographic study of the above-mentioned peculiarities has been implemented in order to define their potential origin(s) and petrogenetic significance.