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Abstract

Chinese onion (Allium chinense G. Don) is an edible vegetable as well as a traditional Chinese medicine. It is rich in steroidal saponins and possesses broad health benefits. In this study, the optimal extraction protocol of the total saponins from Chinese onion (ACS) was explored, and the content of the total steroidal saponins in ACS reached 56.62%. Network pharmacology was applied to predict the related signalling pathways and targets between the main phytochemicals in ACS and hypercholesterolaemia. Enrichment analysis showed that ACS might intervene hypercholesterolaemia through the PI3K-Akt signalling pathway. Meanwhile, cholesterol-lowering effects were verified by ACS intervention in high-cholesterol diet-induced hypercholesterolaemia in Kunming mice. Compared with the model group, the TC and LDL-C levels of mice were decreased and the HDL-C level increased significantly after administration of ACS at a dose of 200 mg kg−1 day−1. The body weight gain, liver index, and atherosclerosis index all decreased dramatically. ACS could significantly reduce the fat content in the liver and reduce the number of fat droplets from the haematoxylin and eosin (H&E) staining of mouse liver. The immunohistochemical staining indicated that ACS could up-regulate the expression of PI3K protein in the liver, thus playing an anti-hypercholesterolaemic role. This study indicated that ACS exhibited significant therapeutic and preventive effects on hypercholesterolaemia, and exerted anti-hypercholesterolaemia through the PI3K-Akt signalling pathway.

Open access

Abstract

Nutritional problems can be seen in children living separated from their families. This cross-sectional pilot study aimed to evaluate intuitive eating, diet quality, and nutritional status of children living separated from their families. A total of 55 adolescent girls aged 12–18 years participated in the study. Anthropometric measurements, biochemical data, and 24-h dietary recall data were collected. The Intuitive Eating Scale-2 (IES-2) and the Turkish version of the Healthy Lifestyle-Diet (HLD-TR) index were used. Participants were divided into two groups according to their scores on the diet quality index using the 50th percentile. Group 1 consisted of participants with a score of 25 points or less, and Group 2 consisted of participants with a score above 25 points. Waist circumference (72.0 (66–83), 65.0 (60–77)) was significantly higher in Group 1 (P < 0.05). IES–2 score (3.05 (2.85–3.40), 3.30 (3.12–3.90)) and two sub–factors; reliance in hunger and satiety cues (2.80 (1.80–3.40), 4.00 (2.60–4.60)) and body–food choice congruence (2.50 (2.00–3.50), 3.50 (3.00–4.50)) were higher in Group 2 (P < 0.05). After adjustment for body mass index, age, and energy intake, there was a significant positive correlation between IES-2 and HLD-TR scores (P = 0.029). In conclusion, it may be advantageous to resort to strategies that promote intuitive eating to reduce obesity and associated problems in this vulnerable group.

Open access
European Journal of Microbiology and Immunology
Authors:
Navid Dashti
,
Forough Golsaz-Shirazi
,
Haleh Soltanghoraee
,
Amir-Hassan Zarnani
,
Mehdi Mohammadi
,
Danyal Imani
,
Mahmood Jeddi-Tehrani
,
Mohammad Mehdi Amiri
, and
Fazel Shokri

Abstract

Background

Waning immunity and emergence of new variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), highlight the need for further research in vaccine development.

Methods

A recombinant fusion protein containing the receptor-binding domain (RBD) fused to the human IgG1 Fc (RBD-Fc) was produced in CHO-K1 cells. RBD-Fc was emulsified with four adjuvants to evaluate its immunogenicity. The RBD-specific humoral and cellular immune responses were assessed by ELISA. The virus neutralizing potency of the vaccine was investigated using four neutralization methods. Safety was studied in mice and rabbits, and Antibody-Dependent Enhancement (ADE) effects were investigated by flow cytometry.

Results

RBD-Fc emulsified in Alum induced a high titer of anti-RBD antibodies with remarkable efficacy in neutralizing both pseudotyped and live SARS-CoV-2 Delta variant. The neutralization potency dropped significantly in response to the Omicron variant. RBD-Fc induced both TH2 and particularly TH1 immune responses. Histopathologic examinations demonstrated no substantial pathologic changes in different organs. No changes in serum biochemical and hematologic parameters were observed. ADE effect was not observed following immunization with RBD-Fc.

Conclusion

RBD-Fc elicits highly robust neutralizing antibodies and cellular immune responses, with no adverse effects. Therefore, it could be considered a promising and safe subunit vaccine against SARS-CoV-2.

Open access

Abstract

Acquired benign tracheoesophageal fistulas and bronchoesophageal fistulas (TEF) are typically associated with granulomatous mediastinal infections, 75% of which are iatrogenic. Candida albicans and Actinomyces are commonly occurring organisms, but are uncommon etiologies of TEF. Normal colonization and the slow growth characteristics of some species of these agents rarely result in infection, mycetoma, and broncholithiasis, and thus, delays in diagnosis and treatment are likely. Few reports describe C. albicans or Actinomyces spp. as the etiology of TEF or broncholithiasis. Herein, we report a case of benign acquired TEF secondary to coinfection of Candida and Actinomyces complicated by the formation of an actinomycetoma and broncholithiasis and a comprehensive literature review to highlight the unique nature of this presentation and offer a diagnostic algorithm for diagnosis and treatment of TEFs. Following a presentation of three months of productive cough, choking sensation, night sweats, and weight loss, a bronchoscopy revealed a fistulous connection between the esophagus and the posterior right middle lobe. Pathology identified a calcified fungus ball and a broncholith secondary to the co-infection of Candida and Actinomyces. This unique presentation of Candida and Actinomyces co-infection and the associated diagnostic algorithm are presented as education and a useful tool for clinicians.

Open access

Xanthoria ectaneoides, usually recognised by its secondary sublobules, is circumscribed in a strict sense using spore size and a molecular phylogeny based on ITS-sequences. The species, earlier considered a synonym of X. aureola, forms a subclade with X. coomae and the new species X. pylyporlykii, described here, whereas X. aureola is positioned in the Xanthoria calcicola subclade. The new species X. pylyporlykii is characterised by a combination of characters typical either for Xanthoria ectaneoides or X. coomae.

Open access

New Records for the Bryophyte Flora of Vietnam, 5

Epihyllous liverworts of Tam Đảo Mountains, Vietnam

Acta Botanica Hungarica
Author:
T. Pócs

In November 1998, guided by Prof. Trấn Ninh we revisited the Tam Đảo mountain range, extensively researched by him before. Since than it was converted into a National Park, with areas stretching to three provinces, Vĩnh Phúc, Thái Nguyên, and Tuyên Quang. The previous collections indicated that many more novelties can be expected from there. As a result, we collected 38 epiphyllous liverwort species. Among these 6 were new to the flora of Vietnam: Cololejeunea fructu-marginata, C. papillosa, C. spathulifolia, Colura bisvoluta, Lejeunea dipterota and Microlejeunea sechuanensis. One species is new to science: Cololejeunea dinhensis. Furthermore, Cololejeunea rotundilobula proved to be a new synonym of Cololejeunea sigmoidea.

Open access

Abstract

Infectious diseases pose a formidable global challenge, compounded by the emergence of antimicrobial resistance. Consequently, researchers are actively exploring novel antimicrobial compounds as potential solutions. This endeavor underscores the pivotal role of methods employed for screening and evaluating antimicrobial activity—a critical step in discovery and characterization of antimicrobial agents. While traditional techniques such as well-diffusion, disk-diffusion, and broth-dilution are commonly utilized in antimicrobial assays, they may encounter limitations concerning reproducibility and speed. Additionally, a diverse array of antimicrobial assays including cross-streaking, poisoned-food, co-culture, time-kill kinetics, resazurin assay, bioautography, etc., are routinely employed in antimicrobial evaluations. Advanced techniques such as flow-cytometry, impedance analysis, and bioluminescent technique may offer rapid and sensitive results, providing deeper insights into the impact of antimicrobials on cellular integrity. However, their higher cost and limited accessibility in certain laboratory settings may present challenges. This article provides a comprehensive overview of assays designed to characterize antimicrobial activity, elucidating their underlying principles, protocols, advantages, and limitations. The primary objective is to enhance understanding of the methodologies designed for evaluating antimicrobial agents in our relentless battle against infectious diseases. By selecting the appropriate antimicrobial testing method, researchers can discern suitable conditions and streamline the identification of effective antimicrobial agents.

Open access

Abstract

The Giant Elm Bracket (Rigidoporus ulmarius) is a widely-distributed necrotrophic polypore species that causes white heart rot in deciduous trees. Despite its recognition as one of the largest species known for forming basidiomata, this perennial polypore had not been documented in Hungary. However, in recent years, two specimens macroscopically resembling this species were collected on old horse chestnut (Aesculus hippocastanum) trees from two different places in Hungary by amateur mycologists. In this study, subsequent morphological and molecular-genetic analyses of these fungal samples confirmed their identity as R. ulmarius. This study represents the first documented occurrence of this plant pathogenic polypore species in Hungary.

Open access
Acta Phytopathologica et Entomologica Hungarica
Authors:
Marianna I. Zhukovskaya
,
Inna V. Grushevaya
,
Alexander A. Miltsen
,
Oksana G. Selitskaya
,
Anna V. Shchenikova
,
Andrei N. Frolov
, and
Miklós Tóth

Abstract

The European corn borer, Ostrinia nubilalis (Hubner), relies on multimodal sensory information to find food, mates, mating and ovipositional grounds. Successful phytosanitary monitoring demands for the bait for the field traps to obtain the most reliable representation of pest abundance. Attraction to light and blend of key components of host plant odor, was tested both in the laboratory and field conditions. Ultraviolet light, which was the most effective in the wind tunnel experiments, was further tested in the field alone and in combination with bisexual lure. Bisexual lure, being attractive in the lab, as well as in the field, did not improve responses to ultraviolet in both experimental designs. All three baits attracted significantly more females than males in the field. Wind tunnel experiments revealed that ultraviolet elicited the shortest response latencies either alone or paired with the odor bait. The lack of synergistic effect between attractive light and odor stimuli is an important issue for pest monitoring. The possible reasons for the observed lack of synergy are the hierarchy of behavioral responses to different stimuli or the intensities of both stimuli are critically important for attractivity of combined stimulus and differ from separately presented ones.

Open access
European Journal of Microbiology and Immunology
Authors:
Cosme Alvarado-Esquivel
,
Sergio Estrada-Martínez
,
Agar Ramos-Nevarez
,
Ángel Osvaldo Alvarado-Félix
,
Sandra Margarita Cerrillo-Soto
,
Gustavo Alexis Alvarado-Félix
,
Carlos Alberto Guido-Arreola
, and
Leandro Saenz-Soto

Abstract

Abdominal pain has been rarely reported in individuals infected with Toxoplasma gondii (T. gondii). The aim of this study was to determine the association between T. gondii infection and abdominal pain. Two hundred and ninety-nine patients with abdominal pain (cases) and 299 age- and gender-matched people without abdominal pain (controls) were tested for anti-T. gondii IgG and IgM antibodies. Twenty-four (8.0%) of the 299 cases and 12 (4.0%) of the 299 controls were positive for anti-T. gondii IgG antibodies (OR: 2.08; 95% CI: 1.02–4.25; P = 0.03). The seroprevalence of T. gondii infection was significantly higher in women with frequent abdominal pain than in women without this clinical feature (OR: 2.30; 95% CI: 1.06–4.96; P = 0.02). Twelve (4.0%) of the 299 cases and 7 (2.3%) of the 299 controls had high (>150 IU mL−1) anti-T. gondii IgG antibody levels (OR: 1.74; 95% CI: 0.67–4.49; P = 0.24). Seven (29.2%) of the 24 cases with anti-T. gondii IgG antibodies and 3 (25.0%) of the 12 controls with anti-T. gondii IgG antibodies were positive to anti-T. gondii IgM antibodies (OR: 1.23; 95% CI: 0.25–5.97; P = 1.00). Results suggest an association between T. gondii infection and frequent abdominal pain. Further research to confirm this association should be conducted.

Open access