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Abstract

Transcription factors of the nuclear factor kappa‐light‐chain‐enhancer of activated B cells (NF-ĸB) family control important signaling pathways in the regulation of the host innate immune system. Various bacterial pathogens in the human gastrointestinal tract induce NF-ĸB activity and provoke pro-inflammatory signaling events in infected epithelial cells. NF-ĸB activation requires the phosphorylation-dependent proteolysis of inhibitor of ĸB (IĸB) molecules including the NF-ĸB precursors through ubiquitin-mediated proteolysis. The canonical NF-ĸB pathway merges on IĸB kinases (IKKs), which are required for signal transduction. Using CRISPR-Cas9 technology, secreted embryonic alkaline phosphatase (SEAP) reporter assays and cytokine enzyme-linked immunosorbent assay (ELISA), we demonstrate that the actin-binding protein cortactin is involved in NF-ĸB activation and subsequent interleukin-8 (IL-8) production upon infection by Helicobacter pylori, Salmonella enterica and Pseudomonas aeruginosa. Our data indicate that cortactin is needed to efficiently activate the c-Sarcoma (Src) kinase, which can positively stimulate NF-ĸB during infection. In contrast, cortactin is not involved in activation of NF-ĸB and IL-8 expression upon infection with Campylobacter species C. jejuni, C. coli or C. consisus, suggesting that Campylobacter species pluralis (spp.) induce a different signaling pathway upstream of cortactin to trigger the innate immune response.

Open access

Abstract

Despite vaccine availability, the global spread of COVID-19 continues, largely facilitated by emerging SARS-CoV-2 mutations. Our earlier research documented that a specific combination of plant-derived compounds can inhibit SARS-CoV-2 binding to its ACE2 receptor and controlling key cellular mechanisms of viral infectivity. In this study, we evaluated the efficacy of a defined mixture of plant extracts and micronutrients against original SARS-CoV-2 and its Alpha, Beta, Gamma, Delta, Kappa, and Mu variants. The composition containing vitamin C, N-acetylcysteine, resveratrol, theaflavin, curcumin, quercetin, naringenin, baicalin, and broccoli extract demonstrated a highest efficacy by inhibiting the receptor-binding domain (RBD) binding of SARS-CoV-2 to its cellular ACE2 receptor by 90%. In vitro exposure of test pseudo-typed variants to this formula for 1 h before or simultaneously administrated to human pulmonary cells resulted in up to 60% inhibition in their cellular entry. Additionally, this composition significantly inhibited other cellular mechanisms of viral infectivity, including the activity of viral RdRp, furin, and cathepsin L. These findings demonstrate the efficacy of natural compounds against SARS-CoV-2 including its mutated forms through pleiotropic mechanisms. Our results imply that simultaneous inhibition of multiple mechanisms of viral infection of host cells could be an effective strategy to prevent SARS-CoV-2 infection.

Open access

Abstract

A number of methods have been applied to measure total antioxidant capacity (TAC), including FRAP, which is based on reducing the amount of iron ions in a complex compound. Researchers often use measurement of absorbance 10 min after mixing a sample with the FRAP reaction solution to calculate TAC. The FRAP solution has been shown to alter absorbance over time by ca 0.0010–0.0020 per hour, under storage conditions. This article intends to show that some substances do not fully or sufficiently react within the common analysis period. It is evident from the results that some substances react more quickly and others very slowly. Absorbance in relation to various phenols was measured. Compared to the levels of absorbance at 10 min, mean absorbance at 48 h was higher by 5,395% for vanillin, 426% for caffeic acid, 170% for sinapinic acid, 67% for gallic acid, 19% for syringic acid, and only by 4% for Trolox. Results for vanillin and caffeic acid indicate potential auto-catalysis.

Open access

Abstract

Hungarian fruit vinegars were characterised in terms of physicochemical attributes (total polyphenol content, antioxidant characteristics/FRAP, CUPRAC, ABTS/, ascorbic acid content, pH, total soluble solids), sensory profiles, and antimicrobial properties.

Both compositional and sensory profiles showed distinct patterns depending on the type of vinegar (Tokaj wine, balsamic or apple) and the additional fruit used. Balsamic vinegars maturated on rosehip, sea buckthorn, and raspberry showed outstanding antioxidant performances. Rosehip, raspberry, and quince vinegars, as well as vinegars produced from Tokaji aszú and balsamic apple obtained high scores for fruity and sweet notes.

Antimicrobial activities were tested on Gram-negative and Gram-positive organisms, including probiotic bacteria. Generally, only weak activities were obtained, which was attributed to the natural sugar content of the samples, depending on the type of the vinegar and the fruit. Similar results, but more pronounced bacterial growth inhibitions were obtained for probiotic strains, however, some probiotic strains were resistant to at least two of the vinegars. Based on these, balsamic apple, raspberry, rosehip, quince, and sea buckthorn may qualify as potential functional components of probiotic preparations containing some of the strains tested.

Open access

Abstract

Aroma components of wines play an important role in the sensory quality of wines. In our paper we investigate the effect of commercially available yeast nutrients under different fermentation parameters. Caproic acid, caprylic acid, capric acid, and different fatty acid esters were used as markers of the alcoholic fermentation process. The optimal temperature for the fermentation of different white wines was at 15–16 °C, in the case of examined wines lower concentrations of fatty acids and fatty acid esters were found at this temperature. At 25–26 °C fermentation temperature very high concentrations of fatty acids and fatty acid esters were detected. Applying different nitrogen-containing wine additives we managed to achieve better aroma profiles for white wines even using musts of lower quality.

Open access

Abstract

The Research Collaboratory for Structural Bioinformatics Protein Data Bank (RSCB PDB) provides a wide range of digital data regarding biology and biomedicine. This huge internet resource involves a wide range of important biological data, obtained from experiments around the globe by different scientists. The Worldwide Protein Data Bank (wwPDB) represents a brilliant collection of 3D structure data associated with important and vital biomolecules including nucleic acids (RNAs and DNAs) and proteins. Moreover, this database accumulates knowledge regarding function and evolution of biomacromolecules which supports different disciplines such as biotechnology. 3D structure, functional characteristics and phylogenetic properties of biomacromolecules give a deep understanding of the biomolecules’ characteristics. An important advantage of the wwPDB database is the data updating time, which is done every week. This updating process helps users to have the newest data and information for their projects. The data and information in wwPDB can be a great support to have an accurate imagination and illustrations of the biomacromolecules in biotechnology. As demonstrated by the SARS-CoV-2 pandemic, rapidly reliable and accessible biological data for microbiology, immunology, vaccinology, and drug development are critical to address many healthcare-related challenges that are facing humanity. The aim of this paper is to introduce the readers to wwPDB, and to highlight the importance of this database in biotechnology, with the expectation that the number of scientists interested in the utilization of Protein Data Bank’s resources will increase substantially in the coming years.

Open access
Agrokémia és Talajtan
Authors: Birkás Márta, Balla István, Gyuricza Csaba, Kende Zoltán, Kovács Gergő Péter, and Percze Attila

A magyar talajművelésben a kezdetektől az 1900-as évek közepéig a hagyományos szántásos rendszerek domináltak. Az ekék tökéletesedése révén a mélyebb szántások hozzájárultak a talajminőség romlásához.

Az 1900-as évek első évtizedeiben a külföldön kidolgozott művelési módszerek még kevesek érdeklődését keltették fel, azonban a szántásnál kedvezőbb körülmények létrehozása érdemi figyelmet keltett.

Az 1970-es évektől a talajvédő művelés Magyarországon is kedvező fogadtatásra talált. Kísérletekkel igazolódott, hogy a direktvetés előnyei – folyamatosság esetén – a hatodik-hetedik évtől észlelhetők. A mulcshagyó művelés kultivátor alkalmazása esetén rövidebb idő alatt nyújtotta a várt talajvédelmi előnyöket, ennek tudható be a gyorsabb terjedése. A kultivátoros művelés értékét a felszínvédő mulcshagyás, a talajminőség megóvás és a biológiailag aktív talaj erősítette meg.

Az időjáráshoz kapcsolható szélsőségek megjelenése az 1980-as évektől újabb művelési megoldások felé fordították a figyelmet. A talajlazítás a vízbefogadás és tárolás, a mulcshagyás, valamint a növények mélyebb gyökerezése révén került a korábbinál szélesebb körű alkalmazásra. A sávos művelési rendszer a nemzetközileg bizonyított eredmények hátterével számos magyar gazdálkodónál is sikeressé vált.

A magyar talajművelés előrehaladásában a talajközpontú szemlélet kiszélesedése, a növényközpontú szemlélet felváltása révén eredményezett kedvező változásokat a talajállapot javulásában.

Tekintettel a talajok sokféleségére és a talajállapot eltéréseire, jelenleg a termőhelyhez, talajhoz adaptált művelési rendszer alkalmazása látszik eredményesnek. Az időjárási szélsőségek fokozódása általában és adott termőhelyen is rangsorba állítja a lehetséges módszereket. A korábban jónak tartott megoldások, beleértve a szántást, ugyanis már egyre kevésbé biztonságosak.

A talajkímélő művelés iránti érdeklődés közel százhúsz évre tekint vissza Magyarországon. Sajátos, de az előrehaladás és a visszatartás tényezői a talajművelésben párhuzamosan jelentek meg az eltelt évek alatt. A művelési előrehaladást visszafogó tényezők között a sok évtized óta fennálló hiedelmek voltak a leginkább hátráltatók, mivel figyelmen kívül maradt a talajvédelem, továbbá a klímaváltozással kapcsolatos veszélyek enyhítésének igénye. Az előrehaladást a talajvédelem felvállalása, a gazdálkodási színvonal emelésének esélye és a klímakár csökkentés kényszere mozdította elő. Az előrehaladást alátámasztó tényezők között legfontosabbak a talajállapot tartós javulása és a klíma eredetű károk enyhítése, továbbá a termés biztonság megtartása és javulása.

Open access

Abstract

As the world is facing numerous global ecological issues at once, the question arises of what will help mitigate and solve contemporary matters related to resource management or climate change without devastating the economies. Fortunately, the widespread application of the circular economy would help countries worldwide simultaneously ensure economic growth without significant environmental deterioration, essentially decoupling the two factors. While Hungary’s contribution to environmental problems is not significant in absolute terms, the economic sector’s circular transition could help the country decrease its impact in relative terms and pave the path for a green economy. Nevertheless, companies, especially SMEs, tend to struggle the most with the initial phases of the shift thus it is crucial to assess the factors that prevent and support their transition.

Open access

Abstract

Potential functional food bakery products were developed and characterized based on White Lupin (Lupinus albus cv. Nelly) flour. Analytical properties of the seeds resemble to previously described Lupinus species, with significantly high protein content (45%). The high protein and dietetic fiber content of the seeds makes Lupin flour suitable to develop potential functional food products with high nutritional values. Results of the development of sweet biscuits and salty crackers enriched with Lupin flour are presented. Sensory evaluation of the bakery products was carried out by 15 panelists using the nine points hedonic scale. Heat stability of White Lupin proteins were investigated by gel-electrophoretic analysis, White Lupin proteins are quite stable at 140°C, after 35 min heating the biscuits still contain 69% of the original amount of proteins. Baking conditions were optimized also based on gel-electrophoretic experiments, the optimal baking time was 30 min at 140°C. Gluten-free Lupin-based biscuits and crackers were produced by completely omitting wheat flour from the recipes.

Open access

Abstract

Wastewater issues became a complex challenge in the world. There are several methods in wastewater treatment, such as chemical, physical, biological, and the combination of each method. However, each process has advantages and disadvantages. The physicochemical methods are common methods used in wastewater treatment, such as adsorption and coagulation. Adsorption and coagulation are excellent methods to remove pollutants. The adsorption process is greatly influenced by pH, adsorbent dose, temperature, and contact time. Coagulant dose, settling time, and pH are the main factors in the coagulation process. Chemical material as an adsorbent and coagulant has been studied in previous research, but recently, to substitution chemical materials is a challenging subject. Natural substances are potential new materials in wastewater treatment and became popular due to their efficiency and environment friendly characteristics. This review investigated the role of adsorption and coagulation in wastewater treatment and the utilization of natural materials as adsorbents and coagulants.

Open access