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Abstract

In this investigation, a rapid and reliable ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) technique was developed for quantification of veliparib in rat plasma and used the method to study the pharmacokinetics and bioavailability of veliparib in rats after oral (6 mg kg−1) and intravenous (2 mg kg−1) administration. Plasma samples were protein precipitated with acetonitrile using midazolam as internal standard. A UPLC HSS T3 chromatographic column was utilized for separation, with a mobile phase consisting of methanol-water-formic acid in gradient elution procedure. Quantitative analysis was performed using multiple reaction monitoring in electrospray positive-ion mode. Veliparib exhibited excellent linearity within the 1–1,000 ng mL−1 range (r > 0.99). The intra- and inter-day precision of veliparib were both within 15%, and the accuracy ranged from 93.7 to 107.7%. The average recovery was above 86%, and the matrix effect was 89.0–95.8%. The AUC(0-t) values for oral and intravenous administration were 1014.7 ± 42.9 and 647.2 ± 85.2 h ng mL−1, respectively, resulting in a bioavailability of 52.3%. The UPLC-MS/MS method established in this study featured a low sample injection volume, a low quantification limit, a short chromatographic runtime, high sensitivity, and selectivity. The developed method can be used for the pharmacokinetic analysis of veliparib in both preclinical and clinical studies.

Open access

Abstract

The success of Hungarian swimming is underpinned by a preparation and competition system that has been developed for many years, thanks to the professional group of the sports federation and the scientific and practical work of professionals in the sport. In our study, we aim to present the experiences of recent years regarding the domestic application of the Long-Term Athlete Development Program (LTAD) for swimmers. Our results indicate that there are several discrepancies between the application of the LTAD model for Hungarian swimmers and the expected theory. We believe that the success of Hungarian swimmers is partly due to maintaining the basic principles of the LTAD model and comparing it with other nations' swimming preparation plans, working within a system that includes unique elements to ensure the success of this small country.

Open access

Abstract

This paper explores the integration of Rational Emotive Behavior Therapy (REBT) within sports psychology, aiming to enhance athletes' mental health and performance of athletes. Sports psychology, encompassing diverse approaches, prioritizes both the mental well-being and performance of athletes. REBT, a vital component of Cognitive Behavioral Therapy (CBT), revolves around an individual's beliefs influencing emotional, behavioral, and physiological reactions to events. It identifies and replaces irrational beliefs with rational alternatives, as depicted in the GABCDE model, emphasizing the impact of beliefs on reactions to triggering events. Previous research highlighted the detrimental impact of irrational beliefs on athletes' mental well-being, identifying these beliefs as a significant risk factor for the emergence of mental disorders, which in turn lead to compromised performance. The integration of REBT into sports psychology shows promise in reducing anxiety, perfectionism, and irrational beliefs among athletes. Its flexibility allows for swift and powerful interventions, either individually or in group settings, fostering mental resilience and enhancing performance. Sports psychologists and coaches play pivotal roles in recognizing and addressing athletes' irrational beliefs, fostering a rational mindset conducive to stable and positive performance. Integrating REBT into training and performance enhancement offers athletes a stable mental foundation, improving self-confidence and stress management, thereby optimizing sports performance. Overall, the integration of REBT into sports psychology offers a promising avenue to bolster athletes' mental fortitude, optimize performance, and foster a balanced mental landscape, facilitating the attainment of their athletic goals.

Open access
Acta Veterinaria Hungarica
Authors:
Anass Ben Moula
,
Saad Salhi
,
Abdellatif Rahim
,
Mouad Chentouf
,
Marianne Raes
,
Jean Loup Bister
, and
Bouchra El Amiri

Abstract

This study was conducted in two steps to evaluate the influence of freezing methods and natural extracts on cryopreserved ram sperm quality. Initially, the research compared the effects of two freezing methods: liquid nitrogen (LN2) versus −80 °C, on post-thawed ram semen on total and progressive motilities and velocity parameters. Experiment I revealed no significant differences (P > 0.05) between the LN2 and −80 °C freezing methods, indicating similar effects on the analyzed parameters. Experiment II aimed to examine the influence of Spirulina platensis (SP) and Salvia verbenaca (SV) extracts added to egg yolk extender on cryopreserved sperm quality, utilizing the −80 °C freezing method. Various concentrations (1.25, 3.75, 6.25 and 8.75 µg*mL−1) of acetone (Ac-SP and Ac-SV) and hexanoic (Hex-SP), as well as methanolic (MeOH-SV) extracts, were added into the extender. A thorough assessment of post-thawed sperm quality parameters, encompassing motility, velocity parameters, viability, membrane integrity, abnormality and lipid peroxidation was conducted. The outcomes demonstrated that 1.25 and 3.75 g*mL−1 of Ac-SP and Hex-SP and 1.25 µg*mL−1 of AC-SV and MeOH-SV increased the post-thawed ram sperm quality. In conclusion, this study emphasizes the antioxidant properties of SP and SV extracts, highlighting their potential to protect cryopreserved sperm cells from oxidative stress at −80 °C.

Restricted access
Acta Veterinaria Hungarica
Authors:
Piotr Cybulski
,
Katrin Strutzberg-Minder
,
Edyta Michalik
,
Radosław Kondratiuk
, and
Artur Jabłoński

Abstract

Nowadays, the three strongly beta-haemolytic spirochaetes, Brachyspira hyodysenteriae, Brachyspira suanatina and Brachyspira hampsonii are thought to be causative agents of swine dysentery, an economically devastating disease of grow-finish pigs characterised by severe mucohaemorrhagic diarrhoea. B. hyodysenteriae has been reported in most leading swine-producing regions. B. suanatina and B. hampsonii have been successfully recovered from faecal samples collected in a few countries only. The present study was performed in March 2023 on faecal samples originating from nine Polish finisher farms with 6,000 to 18,000 animals in a location. Samples were obtained from 40 diarrhoeic finishers. Nucleic acid extracted from the samples was analysed using multiplex PCR for Brachyspira spp. From a total of nine sample populations examined in our study, the genetic material of B. hampsonii was identified in two. To the best of our knowledge, this is the first report on molecular detection of B. hampsonii on pig farms outside North America, Belgium and Germany. Our research highlights the need for increased focus directed on laboratory testing strategies, the lack of which may perplex swine practitioners and severely hinder a definite diagnosis.

Restricted access

A hideg agglutinin betegség diagnosztikája és ellátási gyakorlata

Diagnosis and treatment of cold agglutinin disease

Hematológia–Transzfuziológia
Authors:
Boglárka Brúgós
,
Zsófia Simon
,
Ildikó Zsigmond Soós
, and
Árpád Illés

A hideg agglutinin betegség az autoimmun hemolitikus anémiák ritka altípusa, egy indolens, low-grade lymphoproliferatív betegség, amely nem meríti ki a B-sejtes lymphoma kritériumait. A keringésben kimutatható a vörösvértestek felszínén lévő „I” antigén ellenes, rendszerint IgM típusú autoantitest. Hideg hatására a pentamer szerkezetű antitest a perifériás keringésben az antigénhez kötődve agglutinációt, súlyosabb esetben komplement aktivációt és hemolízist okoz. Vércsoport-szerológiai vizsgálat során erős C3d-pozitivitás igazolható. A betegség tünetei, a klasszikus „fatigue”, fáradékonyság, gyengeség részben a hemolitikus anémia, részben a komplement aktiváció, proinflammatorikus citokin emelkedés következményei. A hideg indukálta, IgM mediálta agglutináció felelős a betegség vaszkuláris tüneteiért (acrocyanosis). A betegség kezelésében B-sejt depléciós és komplentgátló kezelések javasoltak, de nagy, nemzetközi randomizált klinikai vizsgálatok hiányában a kezelésre vonatkozóan csak ajánlások állnak rendelkezésre. Közleményünkben a betegség kivizsgálására és kezelésére vonatkozó ajánlásokat foglaltuk össze.

Open access

This study assesses the elemental composition of Egyptian glauconite sediments, focusing on potentially toxic elements (PTEs) and macronutrients. The primary aim is to evaluate the feasibility of utilizing these sediments as a natural source of potassium for agricultural purposes, besides conventional chemical fertilizers like potassium sulfate. To quantify elemental content, chemical analysis was employed across five distinct grain size fractions after grinding glauconite rock. The assessment included potassium, calcium, sodium, and PTE concentrations, utilizing potassium chloride (KCl) and ammonium acetate lactate (AL Solution) as single extractants, and the BCR extraction protocol, in addition to measuring the pseudo-total content of these elements. Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES) facilitated a comparative analysis of elemental concentrations. Results indicate PTE concentrations within European Union regulations, with an absence of cadmium. Glauconite samples contain approximately 3–3.3% potassium by weight, alongside significant amounts of essential macronutrients (calcium, magnesium) and micronutrients (copper, nickel, zinc) crucial for agriculture. BCR sequential extraction protocol results closely align with or slightly surpass pseudo-total content results. Notably, the AL Solution demonstrates high efficiency compared to KCl or acetic acid in the first step of the BCR method. BCR sequential protocol provides valuable insights into various elemental forms and potential mobility. Overall, this study reveals that glauconite has the potential to serve as a promising alternative potassium fertilizer without causing adverse environmental impacts.

Open access

LGL-leukémia: Dasatinib mellett molekuláris remisszióban lévő chronikus myeloid leukémia ritka szövődménye a splenomegália hátterében

LGL leukemia: A rare complication of chronic myeloid leukemia in molecular remission with dasatinib in the background of splenomegaly

Hematológia–Transzfuziológia
Authors:
Botond Timár
,
Gábor Szalóki
,
Erik Zajta
,
Imre Bodó
, and
Ágota Szepesi

A nagy granuláris lymphocyta (LGL) leukémia leggyakrabban normál LGL-sejtek expanzióját kiváltó krónikus antigén stimulációt okozó állapotokkal, mint például krónikus vírus infekcióval, autoimmun betegségekkel és elsősorban hematológiai malignitásokkal társul. A bemutatott esetben az LGL-leukémia egy ritka okát, tünetét és diagnosztikai lehetőségeit tárgyaljuk. A várandós nőbetegnél rutin vérvizsgálat során észlelt thrombocytosis és splenomegália hátterében chronikus myeloid leukémia igazolódott. Az interferon terápiával indult kezelést autoimmun pneumonitis miatt, a másodvonalban elindított glivec terápiát pedig súlyos neutropénia és társuló MRSA sepsis miatt kellett elhagyni. Harmadvonalban császármetszés után indított dasatinib terápia 16 hónap elteltével major molekuláris választ eredményezett. A betegség kezdetétől észlelt splenomegalia és neutropenia azonban nem szűnt meg. További kivizsgálás során JAK2, CALR, MPL mutáció nem igazolódott, a beteg a lép biopsziába nem egyezett bele. A splenomegália hátterében végső soron a perifériás vérben észlelt monoclonalis T-LGL-sejtszaporulat és cytotoxikus T-sejtek csontvelői infiltrációja alapján diagnosztizált LGL-leukémia állhat. Míg a klonális LGL lymphocytosis a dasatinib kezeléshez ismerten társuló gyakori jelenség, klinikai tüneteket okozó esetet nem találtunk az irodalomban. Miután az LGL lymphocytosis chronikus myeloid leukémia válaszra kifejtett kedvező hatásáról számolnak be, és betegünknél az LGL-leukémia csak egy könnyen uralható enyhe neutropeniát okozott, így a dasatinib kezelésen egyelőre nem változtattunk.

Open access
Acta Veterinaria Hungarica
Authors:
S. Amitha Banu
,
Khan Sharun
,
K. M. Manjusha
,
Rohit Kumar
,
Obli Rajendran Vinodhkumar
,
Ujjwal Kumar De
,
A. M. Pawde
,
Kuldeep Dhama
, and
Amarpal

Abstract

Platelet-rich plasma (PRP) has emerged as a cornerstone in veterinary regenerative medicine. The present study evaluated the impact of the operator on the qualitative and quantitative features of non-activated PRP derived from canine whole blood. Blood was collected in anticoagulant acid citrate dextrose from twelve healthy adult dogs and PRP was prepared according to the double-spin method. Both operators followed an identical protocol and utilized the same equipment for PRP preparation from the pooled blood samples. The resulting PRP underwent characterization, classification and coding based on minimum reporting standards. The consistency and internal reliability of different parameters were also assessed using the intraclass correlation coefficient and Cronbach's alpha values. Variables such as white blood cell (WBC) concentration, relative WBC composition and mean platelet volume (MPV) showed poor reliability, and WBC concentration and MPV also had unacceptable internal consistency. Significant differences were observed in several qualitative and quantitative parameters of the prepared PRP, highlighting the influence of the operator even when the same protocol and equipment were used. Our study has direct implications to regenerative medicine, reinforcing the urgency to set minimum requirements for reporting PRP in research studies.

Restricted access

Abstract

This study investigated the sero-epidemiology of bluetongue in ruminants in North-Western Pakistan. A total of 3,173 serum samples were collected from small (n = 1,651) and large (n = 1,522) ruminants being reared by farmers in 14 districts. Antibodies to bluetongue virus (BTV) were detected using competitive ELISA. The overall prevalence of BTV antibodies was 65%. A significant association (P < 0.05) between the prevalence of BTV antibodies and the risk factors including sex, species, age, area, husbandry practices and breed was shown by univariate analysis. In multivariate analysis, the seroprevalence was 6.5 (95% CL = 3.7–11.4), 5.9 (95% CL = 3.8–9.4) and 2.4 (95% CL = 1.5–3.7) times higher in buffaloes, cattle and goats than sheep, respectively. The seroprevalence was 1.4 (95% CL = 1.1–1.7) times higher in local breeds than in cross/exotic breeds. The seroprevalence was 1.6 (95% CL = 1.1 to 2.3) times higher in sedentary animals than in nomadic animals. The seroprevalence was significantly associated with age. Further work is required to determine the BTV serotypes prevalent in the study area for effective control of the disease.

Restricted access