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Abstract

In this study, the ability of microRNA-1906 (miR-1906) to attenuate bone loss in osteoporosis was evaluated by measuring the effects of a miR-1906 mimic and inhibitor on the cellular toxicity and cell viability of MC3T3‐E1 cells. Bone marrow-derived macrophage (BMM) cells were isolated from female mice, and tartrate-resistant acid phosphatase signalling was performed in miR-1906 mimic-treated, receptor-activated nuclear factor kappa-B (NF-κB) ligand (RANKL)-induced osteoclasts. In-vivo, osteoporosis was induced by ovariectomy (OVX). Rats were treated with 500 nmol/kg of the miR-1906 mimic via intrathecal administration for 10 consecutive days following surgery. The effect of the miR-1906 mimic on bone mineral density (BMD) in OVX rats was observed in the whole body, lumbar vertebrae and femur. Levels of biochemical parameters and cytokines in the serum of miR-1906 mimic-treated OVX rats were analysed. The mRNA expression of toll-like receptor 4 (TLR4), myeloid differentiation primary response 88 (MyD88), p-38 and NF-κB in tibias of osteoporotic rats (induced by ovariectomy) was observed using quantitative reverse-transcription polymerase chain reaction. Treatment with the miR-1906 mimic reduced cellular toxicity and enhanced the cell viability of MC3T3‐E1 cells. Furthermore, osteoclastogenesis in miR-1906 mimic-treated, RANKL-induced osteoclast cells was reduced, whereas the BMD in the miR-1906 mimic-treated group was higher than in the OVX group of rats. Treatment with the miR-1906 mimic also increased levels of biochemical parameters and cytokines in the serum of ovariectomised rats. Finally, mRNA expression levels of TLR4, MyD88, p-38 and NF-κB were lower in the tibias of miR-1906 mimic-treated rats than in those of OVX rats. In conclusion, the miR-1906 mimic reduces bone loss in rats with ovariectomy-induced osteoporosis by regulating the TLR4/MyD88/NF‐κB pathway.

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Abstract

Using alternative fuels (AF) in industry high consuming energy where fossil fuels are largely consumed may be a great solution to decrease CO2 emission and cost production. Or, when using these alternative fuels, the combustion may be difficult to control regarding the different components of AFs compared to fossil fuels. In this case, the use of the computational fluid dynamics CFD tools is a great solution to predict the AFs combustion behavior. This paper represents a computational study of petcoke and olive pomace (OP) co-combustion in a cement rotary kiln burner, established on the commercial CFD software ANSYS FLUENT. This study presents a useful key to choose an adequate simulation model that well predicts co-combustion problems. The performance of the K-ϵ turbulence models varieties (standard, Realizable, and Re-Normalization Group) combined with the hybrid finite rate/eddy dissipation model and the simple eddy dissipation model for predicting the co-combustion characteristics was investigated. The particle phase solutions are obtained using the Lagrangian approach. The performance of the mentioned model was evaluated based on the mesh accuracy, convergence time, temperature shape, and important chemical elements concentration. The predicted values of species concentrations and temperature are compared to the results obtained from the real case study and available literature. The standard K-ϵ model combined with the hybrid finite rate/eddy dissipation model gives the best results and the lower computational resources required for the 2-D model realized.

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In this paper, we investigate a generalization of the classical Stirling numbers of the first kind by considering permutations over tuples with an extra condition on the minimal elements of the cycles. The main focus of this work is the analysis of combinatorial properties of these new objects. We give general combinatorial identities and some recurrence relations. We also show some connections with other sequences such as poly-Cauchy numbers with higher level and central factorial numbers. To obtain our results, we use pure combinatorial arguments and classical manipulations of formal power series.

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A space X is called functionally countable if ƒ (X) is countable for any continuous function ƒ : X → Ø. Given an infinite cardinal k, we prove that a compact scattered space K with d(K) > k must have a convergent k+-sequence. This result implies that a Corson compact space K is countable if the space (K × K) \ ΔK is functionally countable; here ΔK = {(x, x): x ϵ K} is the diagonal of K. We also establish that, under Jensen’s Axiom ♦, there exists a compact hereditarily separable non-metrizable compact space X such that (X × X) \ ΔX is functionally countable and show in ZFC that there exists a non-separable σ-compact space X such that (X × X) \ ΔX is functionally countable.

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We provide necessary and sufficient conditions for the coincidence, up to equivalence of the norms, between strong and weak Orlicz spaces. Roughly speaking, this coincidence holds true only for the so-called exponential spaces.

We also find the exact value of the embedding constant which appears in the corresponding norm inequality.

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Abstract

The aim of this study was to evaluate the prevalence and types of antimicrobial resistance among Gram-negative enteric bacteria isolated from Pelophylax sp. Fifty-four frogs were collected from six provinces in the Eastern Black Sea Region of Turkey. In the cloacal swab cultures, bacteria from 160 different colonies were identified by biochemical tests, automated systems, and matrix-assisted laser desorption ionisation-time of flight mass spectrometry. The antimicrobial susceptibility tests were performed by the disk diffusion method. The observed drug resistance rate was the highest to ampicillin and cefazolin, while the lowest against ciprofloxacin and tetracycline. In the molecular assays, bla TEM (8 Citrobacter spp.), bla SHV (2 Escherichia coli, 1 Hafnia alvei, and a Serratia liquefaciens), tetA genes (E. coli and Klebsiella spp.) and a class 1 integron without any gene cassette (E. coli) were detected. Among the strains, no plasmid-mediated quinolone resistance [qnrA, qnrB, qnrS, qepA and aac (6 ′)-Ib-cr] was found. However, two of three quinolone-resistant Klebsiella strains showed the novel amino acid substitution in the gyrA gene resulting in Ser83Asp and Asp87Glu.The clonality between E. coli isolates was also examined by pulsed-field gel electrophoresis. We consider that multidrug-resistant Gram-negative enteric bacteria in the intestinal microbiota of a cosmopolitan frog species might be a reservoir for antibiotic resistance genes.

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Suppose that K and K' are knots inside the homology spheres Y and Y', respectively. Let X = Y (K, K') be the 3-manifold obtained by splicing the complements of K and K' and Z be the three-manifold obtained by 0 surgery on K. When Y' is an L-space, we use the splicing formula of [1] to show that the rank of HY^(X ) is bounded below by the rank of HY^(Y ) if τ(K 2) = 0 and is bounded below by rank(HY^(Z)) − 2 rank(HY^(Y)) + 1 if τ(K') ≠ 0.

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Abstract

The work which forms the bulk of the present study was carried out on the basis of numerous pieces of field material collected by means of an ethnolinguistic questionnaire in villages inhabited by Burgenland Croats in Western Hungary and Southern Slovakia (where part of the Hungarian territory was annexed after World War II). The field data contain a number of latent and obvious borrowings from Hungarian folk culture. By latent borrowings we mean cultural phenomena that were initially feebly expressed in a particular tradition (and tended to be lost), but during long coexistence with a neighboring heterogeneous tradition they were eventually maintained due to the developed state of the similar phenomena in the neighboring population. We also include here cultural phenomena that are typical of both traditions and have deep roots in the universal model of the naive world view. Analyzing the popular culture and dialects of enclave villages of Burgenland Croats in Hungary and Slovakia, we show that traditional folk culture with the corresponding vocabulary nevertheless acts as an important marker of identity for the population living in a foreign language environment.

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Let k ≥ 1. A Sperner k-family is a maximum-sized subset of a finite poset that contains no chain with k + 1 elements. In 1976 Greene and Kleitman defined a lattice-ordering on the set Sk(P) of Sperner k-families of a fifinite poset P and posed the problem: “Characterize and interpret the join- and meet-irreducible elements of Sk(P),” adding, “This has apparently not been done even for the case k = 1.”

In this article, the case k = 1 is done.

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