Authors:Zhao Namula, Yoko Sato, Manita Wittayarat, Quynh Anh Le, Nhien Thi Nguyen, Qingyi Lin, Maki Hirata, Fuminori Tanihara and Takeshige Otoi
This study was conducted to determine the effects of supplementing the maturation medium with the antioxidant curcumin on the in vitro maturation (IVM), fertilisation and development of porcine oocytes. Curcumin supplementation was performed at concentrations of 0, 5, 10, 20, and 40 µM. At concentrations of 5–20 µM, curcumin had significant positive effects (P < 0.05) on maturation and fertilisation rates compared to the non-treated group. Of the groups cultured with 5–20 µM curcumin, the number of oocytes with DNA-fragmented nuclei after IVM was significantly lower than in groups matured without curcumin. Moreover, curcumin supplementation at 10 µM also gave a significantly higher rate of blastocyst formation compared with oocytes matured without curcumin. Increasing the curcumin concentration to 40 µM yielded negative effects on fertilisation and embryonic development compared with the groups treated with lower concentrations of curcumin. Supplementation with 10 µM curcumin had beneficial effects on the oocyte maturation rate and DNA fragmentation index compared to the non-treated group both in the presence and absence of hydrogen peroxide. These results indicate that curcumin supplementation at a suitable concentration (10 µM) is potentially useful for porcine oocyte culture systems, in terms of protecting oocytes from various forms of oxidative stress.
Authors:Eva Schmal-Filius, Nora Nedorost, Christiane Weissenbacher-Lang and Herbert Weissenböck
The causative role of some infectious agents found in cases of feline pneumonia is under debate, because they are also part of the physiological microbiota of the respiratory tract of healthy animals. In this retrospective study, archived formalin-fixed and paraffin-wax-embedded lung samples of 69 severe and lethal cases of pneumonia in cats were examined by immunohistochemistry (IHC) for the detection of nine selected infectious agents: Pasteurella multocida, Bordetella bronchiseptica, Mycoplasma felis, M. gateae, Chlamydia felis, feline herpesvirus type 1, feline coronavirus, canine distemper virus, and Toxoplasma gondii. The intention was to elucidate their immediate involvement in pneumonia formation. Due to the cross-reactivity of the applied antibodies, a species-specific polymerase chain reaction (PCR) for both targeted Mycoplasma species was applied additionally. In the 42 cases (60.9%) positive for at least one pathogen, several agents were present in a high proportion of the samples (P. multocida – 34.8%, B. bronchiseptica – 29.0%), while others were present in a moderate (feline herpesvirus type 1 – 18.8%, M. gateae – 13.0%, M. felis – 10.1%) or low percentage (T. gondii – 1.4%). All samples were negative for C. felis, feline coronavirus and canine distemper virus. Mixed infections of up to four pathogens were more frequent than single infections. Mycoplasma preferably colonised lung tissue damaged by other pathogens because they never occurred as single infections. Pasteurella multocida, B. bronchiseptica, M. felis, feline herpesvirus type 1 and T. gondii showed abundant replication within lung lesions, thus suggesting a prominent role in pneumonia formation.
Authors:ZS. Molnár, L. Varga, G. Gyenes, Á. Lehotsky, E. Gradvohl, Á.J. Lukács, R.A. Füzi, A. Gézsi, A. Falus and H.J. Feith
Proper handwashing helps prevent the spread of communicable diseases. The aim of our study was to analyse and compare children's knowledge and skills in hand hygiene before and after school interventions in order to evaluate the effectiveness of our peer education programme.
In our longitudinal study, short- and long-term changes in the knowledge, hand-washing skills and health behaviour of 224 lower, upper and secondary school students were assessed. Our measurements were performed with a self-administered, anonymous questionnaire and the Semmelweis Scanner.
As a result of the intervention, the proportion of correct answers increased significantly both in the short term and in the long run compared to the input measurements, but age differences did not disappear for most variables. There is a difference in the process of learning theoretical knowledge and practical skills. Areas not used for handwashing in the paediatric population are different from those described for adults in the literature. There was no significant difference between the mean scores of the right and left hands.
There was a significant positive change in both theory and practice of handwashing. In education, emphasis should also be put on long-term retention of theoretical knowledge in age-specific health promotion programmes within the paediatric population.
Authors:L. Havelda, Z. Bencz and M. Veresné Bálint
The aim of this study was to evaluate the use of probiotic products in Hungary, to explore consumers' knowledge of probiotics, and to gain information about the main characteristics of current usage.
Materials and Methods
The research used a self-developed explorative questionnaire to obtain the necessary information. The data collected was further analysed with IBM SPSS 25.0 and Microsoft Office Excel.
Nearly three-quarters of respondents (73.9%) have used probiotics in their life. Those who have already used these products, mostly heard about them from doctors (62.8%) and friends/relatives (40.5%), while among non-users the main sources of information were web pages (46.8%) and commercials (45.2%).
We found several differences between users and non-users in terms of probiotics awareness. Consequently, healthcare providers may play an important role in the process of careful product choice and proper usage.
Authors:Aymen Mabrouk, Yosra Chebbi, Anis Raddaoui, Asma Krir, Amen Allah Messadi, Wafa Achour and Lamia Thabet
Extensively drug resistant Acinetobacter baumannii (XDR-Ab), has emerged as an important pathogen in several outbreaks. The aim of our study was to investigate the eventual genetic relatedness of XDR-Ab strains recovered from burn patients and environment sites in the largest Tunisian Burn Intensive Care Unit (BICU) and to characterize β-lactamase encoding genes in these strains. Between March 04th, 2019 and April 22nd, 2019 an outbreak of XDR-Ab was suspected. Environmental screening was done. All isolates were screened by simplex PCR for β-lactamase genes. Genetic relatedness was determined by pulsed field gel electrophoresis (PFGE) of ApaI-digested total DNA. During the study period, 21 strains of A. baumannii were isolated in burn patients, mainly in blood culture (n = 7) and central vascular catheter (n = 6). All strains were susceptible to colistin but resistant to imipenem (n = 23), ciprofloxacin (n = 23), amikacin (n = 22), tigecyclin (n = 5) and rifampicin (n = 4). The blaOXA-51-like, blaOXA23, and blaADC genes were present in all strains. These resistance determinants were associated with blaPER-1 in 10 strains. The ISAba1 was inserted upstream of blaOXA-23 in all isolates. PFGE revealed two major clusters A (n = 11) and B (n = 5). This is the first description in Tunisia of clonally related PER-1 producing XDR-Ab in burn patients with probable environmental origin.
Authors:G. Tóth, N. Szentmáry, G.L. Sándor, B. Csákány, Z. Antus, M.T. Pluzsik, O. Lukáts and Z.Z. Nagy
Our aims were to evaluate the primary and clinical evisceration indications and to analyse orbital implant related complications.
We included in our retrospective review all eviscerations between 2006 and 2016 at the Department of Ophthalmology of Semmelweis University, Budapest, Hungary. Primary evisceration indications were classified into six groups: trauma, surgical diseases, infections or inflammations, systemic diseases, tumours and unclassifiable diseases. Clinical immediate evisceration indications were also classified into six groups: painful blind eye due to glaucoma, atrophia/phthisis bulbi, endophthalmitis, cosmetic reasons, acute trauma and expulsive bleeding.
Evisceration was performed in 46 eyes of 46 patients (54.3% males, age 43.0 ± 18.6 years). The most common primary evisceration indications were trauma (37%), surgical diseases (34.8%), infection or inflammation (10.9%), systemic diseases (6.5%), tumours (8.7%) and unclassifiable diseases (2.2%). Painful blind eye due to glaucoma (34.8%) was the most common clinical indication for evisceration, followed by atrophia/phthisis bulbi (26.1%), endophthalmitis (17.4%), cosmetic reasons (13.0%), acute trauma (6.5%) and expulsive bleeding (2.2%). After evisceration, 91.3% of the patients received orbital implant and during 26.8±28.9 months follow-up implant related complications were found in 14.3% of the cases, including implant extrusion (4.8%), partial wound dehiscence (4.8%), implant exposure (2.4%) and orbital inflammation (2.4%).
Painful blind eye and atrophia/phthisis bulbi due to ocular trauma and surgical diseases represent the most common indications for ocular evisceration. If malignant intraocular tumours can be excluded, evisceration surgery combined with a silicon-based orbital implant is a safe and effective procedure.
Authors:T. Valasek, E. Vágó, ZS. Danielisz, M. Babka and É. Kovács
The purpose of the present cross-sectional study is to examine the degree of degenerative patho-anatomical changes in the thoracic vertebrae in asymptomatic adult patients.
Materials and Methods
A total of 150 adult patients were examined with computed tomography (CT) because of various health conditions (e.g., tumour risk). The images were revised with post-processing procedures to detect bony changes in the thoracic vertebrae. Three types of degenerations (osteophytes, arthrosis, and irregular endplates) were examined and graded using appropriate grading systems. Correlational investigations were carried out in relation to age, BMI, and degenerations. Moreover, to examine the value of the degenerations the frequencies of the grading categories were assessed in each segment.
The total number of the patients included was 41, who had no trunk symptoms. We found no convincing correlations in terms of age, BMI, and degenerations, however, age and facet joint arthrosis showed a tentative association. The degree of the degenerations was the largest in the Th7-8, Th8-9, Th9-10 segments for osteophytes, in the Th4-5, Th5-6 for arthrosis, and in the Th8-9, Th9-10 for irregular endplates.
This study found that there are several progressive degenerative changes in the thoracic spine without any clinical symptoms. Accordingly, it can be advised that clinicians should avoid labelling the disorders and planning their treatment based on the results of diagnostic imaging only.
Authors:Dániel Fabó, Zoltán Horváth, Péter Klivényi and Anita Kamondi
Összefoglaló.Bevezetés: A COVID–19-járvány világszerte hónapokra
átalakította a járóbeteg-ellátás működését is. Magyarországon a 2020. március
11-től 2020. június 17-ig fennálló egészségügyi veszélyhelyzeti rendelkezések
szabták meg az új kereteket. Célkitűzés: Az első
veszélyhelyzeti periódus második felében, 2020. április 22. és 2020. május 5.
között mértük fel az epilepsziaellátásban részt vevő orvosok véleményét, hogy
milyen mértékben változott a betegek ellátása, és hogyan élték meg a
változásokat személyesen. Módszer: Internetes kérdőíves
véleményfelmérés történt, a Magyar Epilepszia Liga 2020. április 16–17-re
tervezett, de a COVID–19-járvány miatt elhalasztott XV. kongresszusára
regisztrált neurológusok között. Kilenc egyszeres vagy többszörös
feleletválasztós kérdés és ’szabad kommentár’ mezők álltak rendelkezésre.
Eredmények: A megkeresett 116 neurológus közül 33-an
válaszoltak (28%), összesen 30 kommentár került rögzítésre. 73%-uk szerint a
változások komoly nehézséget okoztak, 15%-uk gondolta, hogy ennek súlyos
következményei lesznek. Új betegek fogadása 53%-ban leállt, 25%-ban nagy
nehézségekbe ütközött. A gondozott betegek problémáit 49%-ban a távvizit
lehetőségeivel élve meg tudták oldani, de 24%-ban ez nem sikerült. A beteg
távollétében lebonyolított vizitek 68%-a dokumentált telefonbeszélgetések
formájában zajlott. Az orvosok kétharmada veszélyeztetve érezte magát, hogy
elkapja a vírust, ebből 40% úgy érezte, nem kap elegendő védelmet, 6% (2 fő)
kapta el a fertőzést. Következtetés: A COVID–19-járvány a
leginkább az új szakvélemények kiadását érintette, de a gondozási feladatokat
sem mindig lehetett megfelelően megoldani. A károkat jelentősen enyhítette az
ellátószemélyzet rugalmassága. A telefonvizitek, szükség esetén, az
epileptológiában pótolhatják a személyes orvos-beteg találkozásokat. A járvány
visszatérésének veszélye miatt a távvizit-alkalmazások technikai fejlesztése és
ezek dokumentálási kérdéseinek megoldása fontos. A járványidőszakban a
személyzet védelmére nagy figyelmet kell fordítani a fertőződés elkerülése és az
orvosok biztonságérzetének fokozása érdekében. Orv Hetil. 2020; 161(46):
Summary.Introduction: COVID-19 pandemic has transformed the operation
of outpatient care worldwide for months. The new framework was set in Hungary by
the health emergency regulations that existed from 11. 03. 2020 to 17. 06. 2020.
Objective: In the second half of the emergency period,
between 22. 04. 2020 and 05. 05. 2020, we surveyed the opinion of physicians
involved in epilepsy care about the extent to which patient care had changed and
how they experienced the changes in person. Method: An internet
questionnaire survey was conducted among neurologists registered for the annual
congress of the Hungarian Chapter of the International League Against Epilepsy.
Nine single- or multiple-choice questions and ‘free comment’ fields were
available. Results: Of 116 neurologists contacted, 33 responded
(28%), and a total of 30 comments were recorded. 73% said the changes caused a
serious difficulty, 15% thought it would have serious consequences. Reception of
new patients was stopped in 53%, and 25% encountered great difficulties. In 49%,
the problems of the cared patients could be solved using remote visits, but 24%
could not solve them properly. 68% of outpatient visits took the form of
documented telephone conversations. Two-thirds of doctors feared catching the
virus, 40% of whom felt they were not getting enough protection. 6% caught the
infection. Conclusion: The COVID-19 pandemic has mostly
affected the issuance of new expert opinions, but care tasks have not always
been adequately addressed. The damage was significantly mitigated by the
flexibility of the care staff. Telephone visits, if necessary, can replace
personal doctor-patient encounters in epileptology. The technical development of
remote visit applications and their documentation issues are important. During
the pandemic period, great care must be taken to protect staff in order to avoid
infection and increase the sense of safety of doctors. Orv Hetil. 2020; 161(46):