Authors:B. Oláhné Horváth, E. Fazekas, N. Kellner and I. Magyar
Medium chain fatty acids are candidates of partial sulphur dioxide replacement in wine, as a solution to the growing consumer concerns about chemical additives. In botrytised sweet wine specialties, large amount of sulphur dioxide addition is one of the effective practices to stop alcoholic fermentation. Increasing medium chain fatty acid levels up to 80 mg l-1 was tested as a sole inhibitor on solid agar surface. S. bacillaris seemed to be the most sensitive, S. cerevsisiae and S. bayanus were more tolerant, while Z. bailii showed the highest tolerance. Then, increasing medium chain fatty acid levels up to 40 mg l-1 combined with 100 mg l-1 sulphur dioxide was introduced into a Tokaj Essence under refermentation. After 56 days, the highest dosage had pronounced effect on the yeast population, but the refermentation was not inhibited completely. Medium chain fatty acids have varying inhibitory effect on botrytised wine-related yeasts, moreover, it could be used effectively in media with high ethanol content, unlike Tokaj Essence.
The influences of dried
powder (MCP) on quality and sensory properties of sucuks were determined. Sucuks were prepared by adding 0% (control), 1.5% MCP, 3% MCP, 0.02% butylated hydroxytoluene (BHT), and sodium nitrate/nitrite (0.01% NaNO
) and lipid oxidation, colour, texture, and sensory features were analysed. Sucuks with the addition of MCP had much lower (P<0.05) peroxide values than with the addition of BHT and nitrate/ nitrite and the control samples. It was found that the L* and a* values of sucuks with the addition of 1.5% and 3.0% MCP decreased significantly (P<0.05) compared to sucuks with nitrate/nitrite and BHT addition and control samples during fermentation. Hardness and chewiness values decreased with the addition of MCP (P<0.05) during fermentation. The sucuks with MCP added at 1.5% and 3% showed the highest overall sensory acceptance. Thus, proper amount of dried MCP addition may be effective on enhancing sucuk quality properties.
Authors:J.A. Pino, E. Sauri-Duch, O. Sosa-Moguel, C.A. Can-Cauich, V.M. Moo-Huchin and L. Cuevas-Glory
An aroma distillate with the odour note described as ‘fresh Habanero chilli pepper' was obtained from hydrodistillation of the fruit. GC-MS analysis of the volatile constituents from the aroma distillate allowed the identification of 100 compounds, most of them esters followed by aldehydes, alcohols, terpenes, ketones, and acids. Encapsulation process of the aroma distillate by spray drying was optimised using response surface methodology. Independent variables were inlet air temperature (150-200 °C) and carrier (maltodextrin 10 DE and gum arabic in 2:1 ratio) content (10-20% wb), while response variables were powder moisture and volatiles retention. Moisture content of the powder varied inversely proportional to the air temperature, while the volatile retention was directly related. Retention of volatiles in the powder increased when the carrier content increased, while this factor negatively affected moisture content. Based on the optimisation model of the response variables, the powder with the highest flavour quality was obtained with an air inlet temperature of 200 °C and 20% wb carrier content, with 4% moisture content and 88.6% volatiles retention.
Authors:B. Horváth, F. Peles, A. Szél, R. Sipos, Á. Erős, E. Albert and A. Micsinai
The aim of the study was the identification and characterisation of coagulase-positive Staphylococcus bacteria obtained from food matrices by mass spectrometry and molecular methods. A total of 46 coagulase-positive Staphylococcus isolates were collected from different foodstuffs. The Staphylococcus isolates were identified by MALDI-TOF MS and confirmed by the presence and sequence analysis of the Staphylococcus protein A gene. Staphylococcal enterotoxin genes were also investigated by multiplex PCR. Based on the identification of strains by the MALDI-TOF MS technique and spa-typing, all strains were identified as Staphylococcus aureus. Based on their MS peak profiles, the isolates matched the spectra of three S. aureus reference strains in the Bruker MALDI Biotyper database, with identification scores higher than 1.999 in the case of all 46 (100%) isolates. The isolates showed great genetic variability. Twenty spa types were identified, from which most lineages are capable of colonizing humans. Fifty percent of the strains harboured at least one of four enterotoxin genes (seg, seh, sei, and ser), but none of the classical enterotoxin genes could be detected.
Authors:I. Mitrović, J. Grahovac, J. Dodić, A. Jokić, Z. Rončević and M. Grahovac
The surplus of waste glycerol, by-product of the biodiesel production process, is available at the global market. Some species of the genera Streptomyces have the ability to assimilate glycerol and convert it into valuable metabolic products. In the present study, the ability of Streptomyces hygroscopicus to assimilate waste glycerol and convert it into metabolic compounds with antifungal activity against four phytopathogenic fungi obtained from apple fruit samples expressing rot symptoms, was investigated. Production of antifungal metabolites by S. hygroscopicus was carried out in 3 l stirred tank bioreactor through 7 days. Fermentation was carried out at 27 °C with aeration rate of 1.5 vvm and agitation rate of 100 r.p.m. The aim of this work was to analyse bioprocess parameters and to determine at which stage of bioprocess the production of antifungal metabolites occurs. Activity of the cultivation liquid on two isolates of Alternaria alternata and two isolates of Fusarium avenaceum were determined every 12 h using in vitro well diffusion method. It was found that the maximum production of antifungal metabolites occurred at 108 hour of cultivation. Formed inhibition zones have shown that the produced antifungal metabolites have high efficacy on tested phytopathogenic fungi (inhibition zone diameter higher than 35 mm for all test organisms).
Due to the shift in consumer behaviour, the proportion of well informed, conscious consumers has been growing steadily, and functional foods with their capacity to protect health have been gaining more and more ground. To achieve market success in the field of functional foods, producers should be able to communicate information effectively concerning health issues and their newly developed product should indeed meet consumer expectations. The aim of our study was to identify and define the components of the dimensions of consumer attitudes in Hungary together with the barriers and motivators of health behaviour. Our research also examined how these factors influence consumer willingness to consume functional foods. Our surveys were carried out in focus groups of health-conscious (n=8) and not health-conscious (n=8) consumers. Our findings confirmed the adequacy of the international dual model, regarding the attitudes of Hungarian consumers towards functional food. Moreover, we also explored the most popular health food categories and sources of information on nutrition.
Authors:A. Kumar, J.P.S. Gill, J.S. Bedi and P.K. Chhuneja
Indiscriminate use of antibiotics in apicultural practices may lead to contamination of otherwise healthy and naturally produced honey. Contamination not only affects honey quality but also pose significant health risks to consumers. In this context, one hundred raw honey samples from India were analysed for presence of antibiotic residues. For determination of oxytetracycline and erythromycin, high performance liquid chromatography and for chloramphenicol, enzyme immunoassay based validated procedures were used. Oxytetracycline and erythromycin with concentrations above maximum tolerance limits were detected in 24% and 2% samples, respectively. None of the samples contained chloramphenicol residues. Although, total dietary intake of detected antibiotics through honey was found to be <1% of their acceptable daily intake values, the presence of antibiotics in honey is an alarming health concern for people following customary honey feeding. The outcomes underline the need of inter-sectoral approaches to create awareness among beekeepers regarding health risks associated with residues of antibiotics in honey and merits of approved apicultural practices. Therefore, to meet global food safety requirements, continual residue monitoring schemes along with enlightenment of beekeepers on scientific beehive management and risks associated with incautious apicultural practices are of vital importance.
Phosphorus is a major substance that is needed especially for agricultural production or for the industry. At the same time it is an important component of wastewater. At present, the waste management priority is recycling and this requirement is also transferred to wastewater treatment plants. Substances in wastewater can be recovered and utilized. In Europe (in Germany and Austria already legally binding), access to phosphorus-containing sewage treatment is changing. This paper dealt with the issue of phosphorus on the sewage treatment plant in Nitra. There are several industrial areas in Nitra where record major producers in phosphorus production in sewage. The new wastewater treatment plant is built as a mechanicalbiological wastewater treatment plant with simultaneous nitrification and denitrification, sludge regeneration, an anaerobic zone for biological phosphorus removal at the beginning of the process and chemical phosphorus precipitation. The sludge management is anaerobic sludge stabilization with heating and mechanical dewatering of stabilized sludge and gas management. The aim of the work was to document the phosphorus balance in all parts of the wastewater treatment plant - from the inflow of raw water to the outflow of purified water and the production of excess sludge. Balancing quantities in the wastewater treatment plant treatment processes provide information where efficient phosphorus recovery could be possible. The mean daily value of P tot is approximately 122.3 kg/day of these two sources. The mean daily value of P tot is approximately 122.3 kg/day of these two sources. There are also two outflows - drainage of cleaned water to the recipient - the river Nitra - 9.9 kg Ptot/day and Ptot content in sewage sludge - about 120.3 kg Ptot/day - total 130.2 kg Ptot/day.
Authors:Kristóf Roland Horváth and István Kistelegdi
First Hungarian Active House refurbishment won the Active House Award and the Energy Globe Hungary prize in 2017. This paper provides insight into the renewal design process of the typical home from the 70’s under disadvantageous site conditions. Dynamic thermal simulations helped to gain insight into space organization and building envelope concepts and their effects on comfort and energy performance. The Active House Standard was applied to evaluate the calculation results. The most advantageous concept was selected for final design elaboration and construction. The implemented building proved that in the refurbishment process it is possible to achieve highest level of efficiency in operation energy consumption with positive yearly balance by simultaneously being able to rearrange the complete interior space and as a consequence the building shape and envelope into a competitive design at international level.
During sketch design stage for the new block of the University of Pécs, Medical School comfort and lighting simulations were applied to quantify optimization strategies. Simulation cases about shading possibilities, façade glazing ratios and internal heat storage masses evaluate the impact of illumination, solar gains, loads and heat transmission on visual and thermal comfort. The goal was to select the most favorable comfort, coupled with maximum reduction of investment costs. Concepts represent 14% (shading), 10% (reduced wall-window ratio), 11% (slabs without suspended ceilings), and 17% (combined wall-window ratio and thermal mass) improvement in thermal comfort performance, and it was proposed for further design.