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Abstract

In this study, the ability of microRNA-1906 (miR-1906) to attenuate bone loss in osteoporosis was evaluated by measuring the effects of a miR-1906 mimic and inhibitor on the cellular toxicity and cell viability of MC3T3‐E1 cells. Bone marrow-derived macrophage (BMM) cells were isolated from female mice, and tartrate-resistant acid phosphatase signalling was performed in miR-1906 mimic-treated, receptor-activated nuclear factor kappa-B (NF-κB) ligand (RANKL)-induced osteoclasts. In-vivo, osteoporosis was induced by ovariectomy (OVX). Rats were treated with 500 nmol/kg of the miR-1906 mimic via intrathecal administration for 10 consecutive days following surgery. The effect of the miR-1906 mimic on bone mineral density (BMD) in OVX rats was observed in the whole body, lumbar vertebrae and femur. Levels of biochemical parameters and cytokines in the serum of miR-1906 mimic-treated OVX rats were analysed. The mRNA expression of toll-like receptor 4 (TLR4), myeloid differentiation primary response 88 (MyD88), p-38 and NF-κB in tibias of osteoporotic rats (induced by ovariectomy) was observed using quantitative reverse-transcription polymerase chain reaction. Treatment with the miR-1906 mimic reduced cellular toxicity and enhanced the cell viability of MC3T3‐E1 cells. Furthermore, osteoclastogenesis in miR-1906 mimic-treated, RANKL-induced osteoclast cells was reduced, whereas the BMD in the miR-1906 mimic-treated group was higher than in the OVX group of rats. Treatment with the miR-1906 mimic also increased levels of biochemical parameters and cytokines in the serum of ovariectomised rats. Finally, mRNA expression levels of TLR4, MyD88, p-38 and NF-κB were lower in the tibias of miR-1906 mimic-treated rats than in those of OVX rats. In conclusion, the miR-1906 mimic reduces bone loss in rats with ovariectomy-induced osteoporosis by regulating the TLR4/MyD88/NF‐κB pathway.

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Abstract

Anisodus tanguticus (Maxim.) Pascher is an important Tibetan folk medicine and the source of tropane alkaloids (TAs) grown in Qinghai-Tibet Plateau. There are marked differences in quality of A. tanguticus from geographic areas. The aim of present research was to establish a method for the quantitative analysis of TAs coupled with chemometrics analysis to trace geographical origins. Qualitative analysis of TAs in A. tanguticus was carried out using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry and quantitative analysis of TAs in different plant organs from different geographical origin was achieved. Contents of TAs were subjected to the principal component analysis, and orthogonal partial least-squares discriminant analysis. The contents of the three marker compounds (anisodamine, anisodine and atropine) in the roots and acrial parts of A. tanguticus were positive correlated and varied significantly from different geographical origins. Principal component analysis, and orthogonal partial least-squares discriminant analysis results showed excellent discrimination between different geographical origin of A. tanguticus. This study could provide comprehensive evaluation and further utilization of A. tanguticus resources.

Open access

Abstract

Climate change has become a real challenge in different fields, including the building sector. Understanding and assessing the impact of climate change on building energy performance is still necessary to elaborate new climate-adaptive design measures for future buildings. The building energy consumption for heating and cooling is mainly related to the building envelope thermal performance. In this study, the winter heat loss and summer heat gain indicators are proposed to assess and analyse the potential impact of climate change on opaque building envelope elements for different climate zones in Morocco over the next 40 years. For that purpose, a one-dimensional heat transfer model is used to simulate the heat transfer through the multi-layer structure of the wall/roof. A medium climate change scenario is considered in this study. The results showed that the current average walls and roof summer heat gain is expected to increase of about 19.2–54.3% by the 2060s depending on the climate zone, versus a less important decrease in winter heat loss varies between –10.6 and –20.6%. This paper provides a reliable evaluation of the climate change impact on building envelope thermal performance, which leads to better adjustments in future building envelope designs.

Open access

Abstract

In this study, a single stage thermoelectric cooler (TER, of size: 21 × 14.2 × 13.5 cm) with thermoelectric module (TEM, of type inbc1-127. 05 with size 40 × 40 × 4.0 mm) and applied electrical power of 30 W and current of 2.5 A, was adopted to estimate the coefficient of performance (COP) of thermoelectric refrigerator (TER). The TER uses a fan to cool the heat exchange region of the TEM. The temperature of the fruit/vegetable samples used in this study was taken before and after cooling for a specific period. The temperatures at both the hot and cold sides of the TEM were also taken at every specific cooling period. The experimented TER can cool vegetable/fruit from about 27 to 5°C within 3 h. The aim of this study is to determine the COP of TER to ascertain the possible applications. The temperature gradient at the heat exchange section of TEM was used to estimate the average theoretical COP to be 0.99, the heat extracted from the cooling chamber and the power supplied was used to estimate the average practical cooling COP to be 0.52; which is within 0.4–0.7 standard COP for a single stage type of TER.

Open access

Abstract

Raw materials requirement is foremost necessary in construction sector. Due to the increase in construction activities, the raw material utilization is also increased, which may lead to depletion of the resources. The usage of M sand also increases day by day. On the other side, waste disposal is posing a major threat to environment and human health. This paper shows the investigation carried out in manufacturing fly ash bricks made by utilizing vermicompost as an alternative material for M sand, the physical and chemical properties of M sand and vermicompost are studied and they seem to be the same. In this study, an attempt is made to check the feasibility of replacement of vermicompost for M sand in brick making. The brick specimens are casted as per the mix proportions and they are tested for strength and durability at the age of 28 days. It has been identified that the vermicompost replacement at 5% and 10%, the compressive strength of the brick is 7.90 and 7.31% respectively, which is found to be nearer to the strength of the control specimen and the water absorption for all the mixes of the brick casted were below 20% as per IS code. Inclusion of vermicompost in the fly ash bricks will tend to reduce the use of M sand.

Open access

Abstract

Digital technologies had an effect on people's lives. The majority of these digital devices rely on cloud storage to meet their memory needs. Hundreds of thousands of images, videos, and audio files are being transferred to cloud storage. Thousands of people around the world access these media every second. Unauthorized access to these media must be avoided. One of the weak points for data breaches is the user-end encryption. This paper suggests a strategy for improving cloud data protection by combining the AES and blowfish encryption and decryption algorithms. AES-256 is used as the first layer, followed by blowfish as the second layer, in the hybrid solution. The output of the first layer is input to the second layer and the final result is analyzed. The proposed method also discusses other combined approaches such as AES with other traditional algorithms but the proposed method gives significant results compared to other approaches.

Open access
Journal of Behavioral Addictions
Authors: Małgorzata Draps, Guillaume Sescousse, Mateusz Wilk, Katarzyna Obarska, Izabela Szumska, Weronika Żukrowska, Aleksandra Majkowska, Ewelina Kowalewska, Julia Szymanowska, Urszula Hamerska, Magda Trybuś, Karolina Golec, Iwona Adamska, Karol Szymczak, and Mateusz Gola

Abstract

Background and aims

Despite the inclusion of the Compulsive Sexual Behavior Disorder (CSBD) in the International Classification of Diseases, very little is known about the underlying affective and cognitive processes. To fill this gap, we compared CSBD subjects and Healthy-Controls (HC) across negative/positive valence, cognitive and sensorimotor systems, as proposed by the Research Domain Criteria framework.

Methods

74 heterosexual CSBD and 66 matched HC males were studied with 10 questionnaires and 8 behavioral tasks. Analyses were conducted with frequent and Bayesian statistics.

Results

CSBD individuals showed significantly higher (than HC) punishment sensitivity, anxiety, depression, compulsivity, and impulsivity symptoms. Frequentist statistical analysis revealed significant interaction between subject group and condition in Incentive Delay Task, concerning the strength of motivation and hedonic value of erotic rewards. Bayesian analysis produced evidence for the absence of group differences in Facial Discrimination Task, Risk-Ambiguity Task, and Learning Task. Also, Bayesian methods provided evidence for group differences in the Emotional Stroop Task and the Incentive Delay Task. Sexual Discounting Task, Attentional Network Task, and Stop Signal Task produced mixed results.

Conclusions

Higher punishment sensitivity and impulsivity among CSBD subjects, along with significant interaction between these groups and erotic vs. non-erotic reward processing is in line with previous findings on negative/positive valence alterations in CSBD patients. This result shows that there are similarities to substance and behavioral addictions. The absence of group differences and mixed results related to cognitive and sensorimotor systems raise concerns to what extent CSBD resembles a wide spectrum of impairments observed in disorders, and demand further research.

Open access

Abstract

Background and aims

Sugar is a potentially addictive substance that is consumed in such high levels the World Health Organisation has set recommended consumption limits. To date there are no empirically tested brief interventions for reducing sugar consumption in adult populations. The current study aimed to preliminarily assess the feasibility of recruitment, retention, and intervention engagement and impact of a brief intervention.

Methods

This pre-post study recruited 128 adults from New Zealand to complete a 30-day internet-delivered intervention with in-person and email coaching. The intervention components were derived from implementation intention principles whereby the gap between intention and behaviour was targeted. Participants selected sugar consumption goals aligned with WHO recommendations by gender. To meet these goals, participants developed action plans and coping plans and engaged in self-monitoring. Facilitation was provided by a coach to maintain retention and treatment adherence over the 30 days.

Results

Intervention materials were rated as very useful and participants were mostly satisfied with the program. The total median amount of sugar consumed at baseline was 1,662.5 g (396 teaspoons per week) which was reduced to 362.5 g (86 teaspoons) at post-intervention evaluation (d = 0.83). The intervention was associated with large effects on reducing cravings (d = 0.59) and psychological distress (d = 0.68) and increasing situational self-efficacy (d = 0.92) and well-being (d = 0.68) with a reduction in BMI (d = 0.51).

Conclusion

This feasibility study indicates that a brief intervention delivering goal setting, implementation planning, and self-monitoring may assist people to reduce sugar intake to within WHO recommendations.

Open access
Acta Veterinaria Hungarica
Authors: Ana Carolina Valentim Hespanha, Bruno Watanabe Minto, Marita Vedovelli Cardozo, Mareliza Possa De Menezes, Júlia Banhareli Tasso, and Paola Castro Moraes

Abstract

Hospital infections are of great relevance in human and animal health, and fomites are important in the spread of pathogens in hospital units. The aim of this study was to investigate the frequency of enterobacteria in the operating room of a veterinary hospital, the potential cross-contamination of samples, and to characterise the susceptibility profile of the isolates to antimicrobials. Sixty-five samples were collected from five different surgical procedures. These samples came from the hands and cell phones of the surgical team and pet owners, operating tables, and patients. Species detection was performed through polymerase chain reaction, genetic diversity by pulsed-field gel electrophoresis (PFGE), and susceptibility to antimicrobials through an antibiogram. Escherichia coli and Proteus mirabilis isolates were obtained from eight samples, from the hands of the anaesthesiologist, the pet owner, and the surgeon; the surgeon's, the nurse's and the anaesthesiologist's cell phones, and two surgical tables. Furthermore, PFGE showed high genetic diversity among the isolates, which showed multidrug resistance. The identification of multidrug-resistant E. coli and P. mirabilis on cell phones of the surgical team is a major concern and, although no direct correlation was found, the isolation of these bacteria inside the clean area of the operating room shows the possibility of nosocomial transmission from cell phones to susceptible patients.

Restricted access

Abstract

Our aim is to identify periods of restrictive versus expansionary economic policy in the euro area in the last two decades. We firstly conducted the study for identifying the dominant trend in fiscal policies and then in monetary policies. We studied several fiscal outputs, focusing on the cyclical adjusted primary balance. We also analysed the European long-term and short-term interest rates. The study was conducted for several windows, namely for 3-, 4- and 5-year periods. Additional procedures were conducted for robustness checks, namely the study of structural breaks in the analysed time series as well as a study of them recurring to Markov-Switching Regimes models. For most of the analysed periods and subperiods of the series, we concluded for the presence of expansionary policies either in the fiscal or in monetary European domains. Finally, the results and the analysis of dependencies in the euro area economy favour the evidence that economic authorities in the euro area have sought to coordinate monetary and fiscal policy to stabilise the economy.

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