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Abstract

Based on the current literature, the link between Achilles tendon moment arm length and running economy is not well understood. Therefore, the aim of this study was to further investigate the connection between Achilles tendon moment arm and running economy and the influence of Achilles tendon moment arm on the function of the plantarflexor muscle-tendon unit during running.

Ten male competitive marathon runners volunteered for this study. The participants ran on a treadmill at two running speeds: 3 and 3.5 m s−1. During running the oxygen consumption, lower leg kinematics, electrical activity of plantar flexor muscles, and fascicle behavior of the lateral gastrocnemius were measured simultaneously. On the second occasion, an MRI scan of the right leg was taken and used to estimate the Achilles tendon moment arm length.

There was a negative correlation between running economy and the body height normalized moment arm length at both selected speeds (r = −0.68, P = 0.014 and r = −0.70, P = 0.01). In addition, Achilles tendon moment arm length correlated with the amplitude of the ankle flexion at both speeds (r = −0.59, P = 0.03 and r = −0.60, P = 0.03) and with the electrical activity of the medial gastrocnemius muscle at 3 m s−1 speed (r = −0.62, P = 0.02). Our finding supports the concept that a longer moment arm could be beneficial for distance runners.

Open access

Abstract

In this study, the ability of microRNA-1906 (miR-1906) to attenuate bone loss in osteoporosis was evaluated by measuring the effects of a miR-1906 mimic and inhibitor on the cellular toxicity and cell viability of MC3T3‐E1 cells. Bone marrow-derived macrophage (BMM) cells were isolated from female mice, and tartrate-resistant acid phosphatase signalling was performed in miR-1906 mimic-treated, receptor-activated nuclear factor kappa-B (NF-κB) ligand (RANKL)-induced osteoclasts. In-vivo, osteoporosis was induced by ovariectomy (OVX). Rats were treated with 500 nmol/kg of the miR-1906 mimic via intrathecal administration for 10 consecutive days following surgery. The effect of the miR-1906 mimic on bone mineral density (BMD) in OVX rats was observed in the whole body, lumbar vertebrae and femur. Levels of biochemical parameters and cytokines in the serum of miR-1906 mimic-treated OVX rats were analysed. The mRNA expression of toll-like receptor 4 (TLR4), myeloid differentiation primary response 88 (MyD88), p-38 and NF-κB in tibias of osteoporotic rats (induced by ovariectomy) was observed using quantitative reverse-transcription polymerase chain reaction. Treatment with the miR-1906 mimic reduced cellular toxicity and enhanced the cell viability of MC3T3‐E1 cells. Furthermore, osteoclastogenesis in miR-1906 mimic-treated, RANKL-induced osteoclast cells was reduced, whereas the BMD in the miR-1906 mimic-treated group was higher than in the OVX group of rats. Treatment with the miR-1906 mimic also increased levels of biochemical parameters and cytokines in the serum of ovariectomised rats. Finally, mRNA expression levels of TLR4, MyD88, p-38 and NF-κB were lower in the tibias of miR-1906 mimic-treated rats than in those of OVX rats. In conclusion, the miR-1906 mimic reduces bone loss in rats with ovariectomy-induced osteoporosis by regulating the TLR4/MyD88/NF‐κB pathway.

Restricted access
Acta Chromatographica
Authors: Steven Yeung, Quanlan Chen, Yongbang Yu, Bingsen Zhou, Wei Wu, Xia Li, Ying Huang, and Zhijun Wang

Abstract

Ganoderma lucidum (GL), also known as Reishi or Lingzhi, is a medicinal mushroom widely used in traditional and folk medicines. The extracts made from the fruiting body and spore of naturally grown GL are the most frequently used in commercial products. More than 400 compounds have been identified in GL with the triterpenoids considered to be the major active components. Large variations in the chemical components were reported in previous studies and there is no comprehensive study of the content of multiple major triterpenoids in the GL product. In addition, there is no report in the comparison of chemical profiles in different parts of GL (i.e., fruiting body and spore). Determining the chemical composition and comparing the differences between fruiting body and spore are essential for the identity, efficacy and safety of various GL products.

In this study, 13 compounds (ganoderenic Acid C, ganoderic Acid C2, ganoderic Acid G, ganoderic Acid B, ganoderenic Acid B, ganoderic Acid A, ganoderic Acid H, ganoderenic Acid D, ganoderic Acid D, ganoderic Acid F, ganoderic Acid DM, ganoderol A, and ergosterol) were selected as the chemical markers. The purpose of this study is to develop an HPLC-DAD fingerprint method for quantification of these active components in GL (spore and fruiting body) and test the feasibility of using the HPLC-DAD fingerprint for quality control or identity determination of GL products.

The results showed that this method could determine the levels of the major components accurately and precisely. Among the 13 components, 11 ganoderma acids were identified to be proper chemical markers for quality control of GL products, while ganoderal A was in a very low amount and ergosterol was not a specific marker in GL. The extracts of fruiting body contained more chemical compounds than those of spore, indicating that these 11 compounds could be a better chemical marker for the fruiting body than the spore. The HPLC chemical fingerprint analysis showed higher variability in the quality of GL harvest in different years, while lesser variation in batches harvested in the same year.

In conclusion, an HPLC assay detecting 11 major active components and a fingerprinting method was successfully established and validated to be feasible for quality control of most commercial GL products.

Open access

Abstract

Narciclasine is a 7-hydroxy derivative of lycorisidine. It was the first alkaloid isolated from the stem of narcissus (Amaryllidaceae) in 1967. Six mice were given narciclasine (5 mg/kg) by intravenous administration. A UPLC-MS/MS method was developed to determine narciclasine in mouse blood. Tectorigenin (internal standard, IS) and narciclasine were gradient eluted by mobile phase of methanol and 0.1% formic acid in a BEH C18 column. The multiple reaction monitoring (MRM) of m/z 308.1→248.1 for narciclasine and m/z 301.1→286.0 for IS with an electrospray ionization (ESI) source was used for quantitative determination. The calibration curve ranged from 1 to 6,000 ng/mL. The accuracy was from 92.5 to 107.3%, and the matrix effect was between 103.6 and 107.4%. The developed UPLC-MS/MS method was successfully applicated to a pharmacokinetic study of narciclasine in mice after intravenous administration (5 mg/kg).

Open access

Abstract

Pesticides as one of the micro-pollutants present a great problem and threat to the environment and human health. They can infiltrate the sources of drinking water by application on the agricultural fields. This article is focused on Atrazine, Terbuthylazine and their metabolites. It is their complex structure that makes them hard to degrade naturally and, thus, water needs to be treated before safe using. Therefore, this article studies adsorption on the two granular activated carbons Filtrasorb 400 and Norit 1240 W. For the determination of the concentration liquid chromatography was used. In this article it is presented that Filtrasorb 400 efficiency (26–40% and 33–45% for atrazine and terbuthylazine respectively) is better than the efficiency of Norit 1240 W (9–27% and 10–24% for atrazine and terbuthylazine respectively).

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Abstract

This study is concerned to the investigation of the stability of bridges by taking into account the soil structure interaction and their impact on the dynamic behavior of the structures. The bridge studied is localized at PK 318 + 750 at the national level, between the city of Al Hoceima and Kasseta (Morocco). The analyses are carried out with the ANSYS code demonstrated that for conditions of support, the distribution of displacements and the fundamental frequency for each type of soil change according to its mechanical properties. This work also indicates that the proximity of the fundamental frequencies of the soil structure and strongly influences the soil-structure interaction.

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Abstract

Concrete structures that are influenced by degradation, overloading, the thawing, and freezing cycles, corrosion of reinforced bars, should be repaired or strengthened. Each of the mentioned influences lead to decreased load-carrying capacity of the structure or its member. Exceeding the load-carrying capacity leads to mechanical damage of members or excessive deformation. The damaged member has to be strengthened to the required level of reliability and load-carrying capacity. There exist many types and methods of strengthening the columns. This research work deals with strengthening of columns by concreting a new layer of fiber concrete. When applying that type of strengthening, it is necessary to assure the contact between the old layer and the new one. The paper deals with analysis of different types of contacts and determination of their parametric values.

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Pollack Periodica
Authors: Stepan Shapoval, Nadiia Spodyniuk, Vasyl Zhelykh, Volodymyr Shepitchak, and Pavlo Shapoval

Abstract

In line with the energy development strategy of Europe, it is necessary to implement energy efficient buildings, in which the external enclosures are converters of solar energy to thermal. Therefore, the purpose of the publication was the scientific substantiation and development of the solar heating system with the use of rooftop solar panels. Graphical and analytical dependences of the coefficient of thermal efficiency of the solar panel in heat supply system from the angles of solar radiation incoming and the density of the radiation flux were obtained. Analysis of the results showed that the coefficient of thermal efficiency of the solar panel without transparent coating with the placement of pipelines of the circulation circuit of the coolant over the heat absorber increases by 50%.

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Abstract

Safety valves are the most important safety devices of the pressure system. For safety valves in the vast majority of cases in industrial environment, direct spring-loaded safety valves are used. The most important parameter of the equation of motion is the flow force. The main goal of the analysis was to compare the simulated flow forces with the measured results and validating the computational fluid dynamics model. Simulations were made in ANSYS 2019 R1 code for numerous fixed valve disk positions on different pressures. Results are in good agreement with the measured data.

Open access

Abstract

Concrete indeterminate flexural members represented by continuous beams reinforced with both fiber-reinforced polymers and steel bars in a way that allows for moment redistribution at failure are analyzed. The efficiency of introducing steel bars in the critical sections where plastic hinges are likely to form is evaluated in terms of reliability. Monte Carlo simulation and the concept of comparative reliability are both employed. Ultimately, the effect of different design parameters on the strength reduction factor is evaluated.

Open access