Abstract
Antibiotic resistance constitutes a significant public health challenge, with diverse reservoirs of resistant bacteria playing pivotal roles in their dissemination. Among these reservoirs, pets are carrying antibiotic-resistant strains. The objective of this study was to assess the resistance profiles of Escherichia coli, and the prevalence of extended-spectrum β-lactamase (ESBL) producing E. coli strains in dogs and cats from Tamaulipas, Mexico. A total of 300 stool samples (150 dogs and 150 cats) from healthy pets were subjected to analysis. Antibiotic susceptibility testing and the identification of ESBLs were carried out by disc diffusion method. The presence of resistance genes, class 1, 2, and 3 integrons (intI1, intI2, and intI3) and phylogroups was determined by PCR analysis. The findings reveal that 42.6% (128/300) of the strains exhibited resistance to at least one of the eight antibiotics assessed, and 18.6% (56/300) demonstrated multidrug resistance (MDR), that distributed across 69 distinct resistance patterns. Altogether 2.6% of E. coli strains (8/300) were confirmed as TEM and CTX-M type ESBL producers. These outcomes underscore the roles of dogs and cats in Tamaulipas as reservoirs for the dissemination of MDR and/or ESBL strains. The results underscore the necessity for conducting prevalence studies on ESBL-producing E. coli, forming a foundation for comprehending the present scenario and formulating strategies for the control and mitigation of this issue.
Abstract
Background and aims
The aim of the present study was to estimate the complex association between Internet Gaming Disorder (IGD), substance use, and other risky behaviours in Czech adolescents whilst providing prevalence estimates of IGD and psychometric information regarding the Czech Internet Gaming Disorder Scale–Short-Form (IGDS9-SF).
Methods
A representative sample of 3,950 Czech adolescents was recruited through stratified random sampling in the school setting.
Results
Disordered gamers showed frequent use of specific substances such as pharmaceuticals, methylenedioxymethamphetamine, and lysergic acid diethylamide. In contrast, non-gamers had higher prevalence of alcohol, cigarettes, sedatives and tranquillisers, and marijuana use. A logistic regression, utilising IGDS9-SF raw scores and average daily gaming time, revealed a U-shaped relationship between gaming and both alcohol and cigarette use. Additionally, conduct problems such as bullying, and risky in-game behaviours were more prevalent among disordered gamers, with the exception of forging parents' signatures. The overall prevalence of IGD was 3.62% (95% CI = [3.1%, 4.3%]), with higher rates in males (5.89%; 95% CI = [4.9%, 7.0%]) than in females (1.45%; 95% CI = [1.0%, 2.1%]).
Discussion and conclusions
The Czech IGDS9-SF used in the present study showed adequate psychometric properties. The association between gaming and substance use behaviours may be specific and multifaceted depending on the severity of the gaming-related problems. Furthermore, disordered gamers may become more vulnerable due to a higher incidence of conduct problems, bullying (victimisation), and in-game risky behaviours such as engagement with microtransactions mechanics (e.g., loot box) within video games.
A Régi Zeneakadémia építésének és helyreállításának története
The History of the Construction and Renovation of the Old Academy of Music
Jelen tanulmány összefoglalja és értékeli a Régi Zeneakadémiával foglalkozó szakirodalmi kutatás korábbi eredményeit; elsődleges források alapján rekonstruálja az épület 19. századi építéstörténetét, különös tekintettel a belső terek kialakítására és átalakítására (1881, 1883); foglalkozik a 20. századi történetével, tárgyalva a külső és belső átalakításokat, funkcióváltást és helyreállításának főbb szakaszait és eredményeit az 1903-tól 1986-ig tartó időszakban.
Az Andrássy úton a korabeli műcsarnokkal és a mintarajztanoda épületével egy csoportozatot képező Régi Zeneakadémia terveit Lang Adolf készítette, az építést Wechselmann Ignác építési vállalkozó hajtotta végre. 1879-ben adták át az épületet, amely 1907-ig a hazai zenei oktatás kiemelt helyszíne, Liszt Ferenc, Erkel Ferenc és Stróbl Alajos élete egy rövidebb szakaszának színtere volt. A Régi Zeneakadémia, ellentétben a tömböt alkotó két másikkal, kevésbé kutatott és publikált. Építéstörténetében vannak azonban olyan epizódok (meg nem valósult tervek, események), amelyek az építészeti értékein túl, még történelmi értékekkel is felruházzák. Az épület másfél évszázados története során több külső és belső átalakításon is átesett. A külső átalakítások eredményét ma is látjuk. Az 1980-as évek elején, az 1986-os átadásig, az addig különböző funkcióknak eleget tévő épület belső átalakításának és eredeti funkciójának visszaállítása volt a cél. Mivel nem állnak rendelkezésre eredeti tervek, ezért a főfalak szolgáltak információkkal, az eredeti állapot nehezen rekonstruálható. Az 1980-as évek visszaállító, nagyrekonstrukciós munkálatai mellett még két ismert múlt századi átalakítása volt az épületnek, 1907–1909-ben és 1957-ben. 1903-ban vette meg a Régi Zeneakadémiát Wodianer Arthur, aki az 1907. és 1909. évben kért engedélyt átalakításra. 1957-ben, már a Tannimpex külkereskedelmi vállalat tulajdonában álló épület hangversenytermét kettéosztották (új acélgerendákon nyugvó osztófödém került az első és második emelet közé), a nagyobb termeket kisebb helyiségekre osztották, ekkor épülhetett a lift, a lépcsőház eredeti nyílászárói rovására üvegtégla falat alakítottak ki. Az 1957-es átalakítás eredményéről az 1970-es években készült (Tannimpex tulajdonában) levő felmérési rajzok tanúskodnak. Az 1983-as felújítás során elbontották a másodlagosan épített válaszfalakat, a hangversenyteremben lévő osztófödémet, a nagyterem (mai Kutatókönyvtár) díszítőfestését, a hangversenyteremben a mennyezetet és az eredeti boltozatokat rekonstruálták (az azokat takaró másodlagos szerkezeteket, festést megszüntették), a homlokzati nyílászárókat felújították (a munkák összefoglalása a teljesség igénye nélkül). 1986-tól az épület újra oktatási intézményként működik, a mai napig.
Abstract
The use of antibiotics in agriculture and subsequent environmental pollution are associated with the emergence and spread of multidrug-resistant (MDR) bacteria including Escherichia coli. The aim of this study was to detect antimicrobial resistance, resistance genes and mobile genetic elements of 72 E. coli strains isolated from faeces of healthy farm animals. Disk diffusion test showed resistance to ampicillin (59.7%), tetracycline (48.6%), chloramphenicol (16.7%), cefoperazone and ceftriaxone (13.9%), cefepime and aztreonam (12.5%), norfloxacin and ciprofloxacin (8.3%), levofloxacin (6.9%), gentamicin and amikacin (2.8%) among the studied strains. Antibiotic resistance genes (ARGs) were detected by polymerase chain reaction: the prevalence of bla TEM was the highest (59.7% of all strains), followed by tetA (30.6%), bla CTX-M (11.1%), catA1 (9.7%), less than 5% strains contained bla SHV, cmlA, floR, qnrB, qnrS, tetM. 26.4% of E. coli strains had a MDR phenotype. MDR E. coli more often contained class 1 integrons, bacteriophages, conjugative F-like plasmids, than non-MDR strains. ARGs were successfully transferred from faecal E. coli strains into the E. coli Nissle 1917 N4i strain by conjugation. Conjugation frequencies varied from (1.0 ± 0.1) * 10−5 to (7.9 ± 2.6) * 10−4 per recipient. Monitoring mobile genetic elements of E. coli for antibiotic resistance is important for farm animal health, as well as for public health and food safety.
Néhány gondolat az avar kori fülkesírok értelmezéséhez
Some thoughts on the interpretation of the graves with an end-wall shaft
A fülkesírok eredete már régóta megoldatlan kérdése az avar kor kutatásának. Jelen tanulmányban új szempontokat kívánunk nyújtani a sírforma értelmezéséhez. Ehhez belső- és közép-ázsiai, valamint kelet-európai analógiákat mutatunk be. Ezek a kora avar kori sírokhoz képest jóval korábbiak, néhány esetben egykorúak. A padmalyos sírokkal való együttes előfordulásuk alapján a két típus valószínűleg egymás formai variánsaként értelmezhetők.
This paper addresses several important aspects of the historical spread of the Huayan and Chan Buddhist teachings in the Tangut state, and traces local developments of Chan and Huayan thought among the Tang-uts. These developments, as they emerge from the received texts, indicate not only local developments in Xixia, but also provide broader implications for the study of Sinitic Buddhism in Northern China and Central Asia during 11th–13th centuries. The core of the paper concerns Tangut Huayan commentaries, the Huayan lineage composed by Yixing Huijue, Tangut recensions of texts authored by Guifeng Zongmi and the Tangut texts of Hongzhou Chan Buddhism.
The word T1G2 occurs three times in the Ongi Inscription. The sentences containing T1G2 have been interpreted in a variety of ways, with no single reading thus far being deemed satisfactory. A notable aspect of the Old Turkic script is the presence of two distinct characters for the majority of consonant sounds, one representing a back-vocalic and the other a front-vocalic sign. Given that T1G2 occurs on three occasions, it is plausible that the individual(s) responsible for inscribing the text may have deliberately employed this format. The author hypothesizes that T1G2 in question represents the Turkic rendering of the Chinese character dí 敵, which translates to “enemy; hostile.”
This study examines the influence of Mongolian language on spoken Chinese, with a focus on the Sino-Mongolian, or Chinese language translated using the literal translation style (zhiyi-ti 直譯體) from Mongolian. Sino-Mongolian was a type of contact language, based on the Chinese vocabulary and Mongolian syntactic structure. Common linguistic elements are observed between Sino-Mongolian and the Chinese textbook No Gŏltae edited in the Koryŏ Kingdom under Mongol rule. The analysis confirms that No Gŏltae was not translated from Mongolian language but a type of spoken Chinese that was influenced and shaped by Sino-Mongolian through oral dissemination. Although Sino-Mongolian was an artificially translated language, the literal translation style may have been based on Altaic Pidgin Chinese. Hence, it was readily accepted in Northern China and naturally influenced and shaped the spoken Chinese there. It is concluded that the colloquial Chinese influenced by Sino-Mongolian, as observed in No Gŏltae, would be defined as Mongolo-Chinese, a type of Creole language.
Abstract
Background
Research on individual differences in brain structural features of internet gaming disorder (IGD) and established addictions such as tobacco use disorder (TUD) is currently limited. This study utilized normative modeling to analyze the cortical thickness (CT) development patterns of male patients with IGD and TUD, aiming to provide further insights into whether IGD qualifies as an addiction.
Methods
Surface-based brain morphometry (SBM) was used to calculate CT from T1-weighted magnetic resonance imaging data of 804 male participants (665 healthy individuals, 68 IGD and 71 TUD). Gaussian process regression was employed to generate normative models of CT development. Deviation maps were produced to depict deviations of IGD and TUD participants from the typical developmental patterns.
Results
Both addiction groups exhibited widespread cortical thinning, particularly in regions such as the bilateral temporal pole and medial orbitofrontal cortex. The TUD group demonstrated a higher degree of individualization and limited spatial overlap compared to the IGD group. Opposite trends in CT changes were observed between the two groups in the bilateral pericalcarine cortex and pars triangularis.
Conclusions
These findings regarding the similarities and differences between IGD and TUD provide support for the idea that IGD shares common features with substance-related addictions and contribute to a deeper understanding of the neural mechanisms underlying IGD.
Abstract
Formaldehyde plays a significant role in the global economy due to its unique chemical properties and widespread use in both the medical field and various industrial sectors. However, formaldehyde (HCHO) is classified as a persistent organic pollutant and is known to be harmful. Exposure to formaldehyde, both direct and prolonged, can cause severe health issues and potentially lead to death. It is recognized as a human carcinogen by numerous organizations and can negatively impact the endocrine system, as well as having mutagenic or teratogenic effects. Formaldehyde has been detected in various matrices at levels exceeding those permitted by international health regulatory bodies. Chromatography, particularly high-performance liquid chromatography (HPLC) coupled with detection methods, is an analytical technique frequently used to determine volatile organic molecules, including formaldehyde. This investigation aimed to develop and validate an assay method to identify and quantify formaldehyde in cosmetic products using HPLC-PDA. The chosen isocratic system consisted of a ZORBAX RX-C8 column (250 mm × 4.6 mm; 5 μm) and a mobile phase mixture of acetonitrile and water in a 55:45 (v/v) ratio, with a flow rate of 1.5 mL min−1. Formaldehyde was detected at 353 nm after derivatization with a 0.1N 2,4-dinitrophenylhydrazine solution, with a retention time of approximately 5.0 min and a total run time of 7.0 min for formaldehyde. The method was validated according to ICH Q2 (R1) guidelines. The validation parameters demonstrated high selectivity, precision (RSD <2%), accuracy (mean recovery of 101.3%), linearity (r > 0.995 in the 1,000–2,400 ppm range), and a limit of quantification (LOQ) of 0.1 ppm. The validated analytical method was applied to 35 cosmetic products. None of these products were labeled as containing formaldehyde, although 6 imported products were labeled as containing formaldehyde-releasing preservatives. The results indicated that 88.6% of the samples tested positive for formaldehyde, with free formaldehyde levels ranging from 20 to 981 ppm. All samples contained formaldehyde levels below the 2000 ppm threshold.
