Browse Our Biology and Life Sciences Journals
Biology is a study of living objects and their life processes. It examines all aspects of living organisms such as their occurrence, classification, internal and external structure, nutrition, reproduction, inheritance, etc. The term “biology” is commonly replaced by the terms “life sciences” and “biological sciences.” There are dozens of branches of biology. Some of the major ones include:
Biology and Life Sciences
Abstract
Blends of germinated maize and germinated lentil (100:0, 85:15, 70:30, 55:45, and 40:60) were utilised for preparation of chips by air frying. Higher contents of protein, ash, total phenols, flavonoids and antioxidant activity were confirmed in germinated flours through analysis of FTIR spectra. Doughs from blends containing higher amounts of germinated lentil flour showed improved viscoelastic behaviour indicating better protein network as compared to maize dough. Significant (P ≤ 0.05) variation was observed in colour and hardness of air fried chips prepared from various blends. The 70:30 blend was found most acceptable based on sensory evaluation. Chips from this formulation were found superior to ungerminated maize chips in terms of protein digestibility and mineral composition.
Abstract
Nanoparticles can be synthesised by several methods. Due to the long duration, high cost, and toxic by-products of chemical and physical methods, the biological method has become more preferred. Among various sources such as bacteria, fungi, or yeast, the use of plants in biological synthesis has proven to be the most ideal. Many metals can be used in the biological method, including copper oxide (CuO). In this study, copper oxide nanoparticles (CuONPs) were synthesised using Pimpinella anisum L. aqueous extract. For characterisation of the CuONPs, UV–Visible Spectroscopy (UV–Vis), Scanning Electron Microscopy (SEM), Energy Dispersion Spectroscopy (EDS), and Fourier Transform Infrared Spectroscopy (FT-IR) analyses were performed. The biological activity of the P. anisum extract and CuONPs was determined using DNA cleavage (agarose gel electrophoresis), antioxidant (1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity and hydrogen peroxide scavenging activity), mutagenic (Ames/Salmonella test), and catalytic (methylene blue degradation) activities. In DNA cleavage activity test, CuONPs completely denatured DNA at high concentrations (100 and 200 μg mL−1) due to their oxidative activity. The results showed that both the extract and CuONPs have antioxidant properties in DPPH and hydrogen peroxide scavenging activities. According to the mutagenicity, CuONPs did not have a mutagenic effect. In catalytic activity, CuONPs degraded methylene blue within 240 min by 99.45%.
Abstract
In this study, the odour values of rosemary, mint, black cumin, lavender, and thyme oils were determined by an electronic nose working with an Arduino microprocessor. The components of the plants were compared with the sensor data, and the most sensitive sensors were determined by sorting according to the highest components. The study data indicated that the MQ3 sensor exhibited the highest sensitivity for thyme, black cumin, and lavender oils, which contain 63% carvacrol, 38% thymoquinone, and 36% linalool, respectively. Also, MQ7 was the most sensitive sensor for menthone (68%) in mint oil and eucalyptol (45%) in rosemary oil. In addition, a low-cost and non-contact device that works with an infrared sensor has been developed to detect the identity of the oil added into the vial. The study data showed that low-cost Arduino-based IR and odour sensors can determine the identity and component percentage of oils. The fact that the developed device can detect with 100 percent accuracy even in case of peppermint and rosemary oils, which are very similar in appearance, shows that the study data will be an inspiration for contactless determination of oil quality and type.
Abstract
The effect of different drying methods (oven drying, fluid bed drying, and freeze drying) on the fatty acid composition, astaxanthin content, antioxidant activity, and colour values of giant red shrimp (Aristaeomorpha foliacea) processing wastes were investigated. These results showed that freeze drying was the most effective method in preserving the quality of shrimp processing waste (SPW), resulting in higher levels of EPA and DHA, astaxanthin content, antioxidant activity, and desired reddish colour characteristics compared to other methods. These findings highlight the potential of freeze drying as a suitable technique for converting SPW into high value products.
Abstract
Background
The rising prevalence of fungal infections and challenges such as adverse effects and resistance against existing antifungal agents have driven the exploration of new antifungal substances.
Methods
We specifically investigated naphthoquinones, known for their broad biological activities and promising antifungal capabilities. It specifically examined the effects of a particular naphthoquinone on the cellular components of Candida albicans ATCC 60193. The study also assessed cytotoxicity in MRC-5 cells, Artemia salina, and the seeds of tomatoes and arugula.
Results
Among four tested naphthoquinones, 2,3-DBNQ (2,3-dibromonaphthalene-1,4-dione) was identified as highly effective, showing potent antifungal activity at concentrations between 1.56 and 6.25 μg mL−1. However, its cytotoxicity in MRC-5 cells (IC50 = 15.44 µM), complete mortality in A. salina at 50 μg mL−1, and significant seed germination inhibition suggest limitations for its clinical use.
Conclusions
The findings indicate that primary antifungal mechanism of 2,3-DBNQ might involve disrupting fungal membrane permeability, which leads to increased nucleotide leakage. This insight underscores the need for further research to enhance the selectivity and safety of naphthoquinones for potential therapeutic applications.
Abstract
Tigecycline-resistant Acinetobacter baumannii (TRAB) is increasing in Thailand, complicating antibiotic treatment due to limited antibiotic options. The specific resistance mechanism behind tigecycline resistance is still unclear, necessitating further investigation. We investigated the presence of OXA-type carbapenemases, the antimicrobial susceptibility profile, the inhibitory effect of carbonyl cyanide m-chlorophenylhydrazone (CCCP) on tigecycline susceptibility, the expression levels of RND-type efflux pumps and amino acid substitutions within a two-component regulatory system on 30 Thai clinical isolates. Our investigation revealed that most of (73.3%) TRAB isolates expressed at least one member of the Ade efflux pumps. The ade B was most frequently expressed (63.3%), followed by ade R (50%), ade S (43.3%), ade J (30%) and ade G (10%). Overexpression of the AdeABC was associated with increased tigecycline minimum inhibitory concentrations (MICs) and amino acid substitutions within the AdeRS. Notably, isolates harbouring simultaneous mutations in these genes exhibited an increase in the transcription level of the ade B. Our findings highlight the significant role of the AdeABC system in tigecycline resistance among Thai clinical TRAB isolates. This is supported by point mutations within the AdeRS and upregulated expression of the ade B. These results provide valuable insights for understanding resistance mechanisms and developing novel therapeutic strategies.
Abstract
The present work aimed to study the yeast communities of whole crop corn silages (CS) that were previously contaminated with aflatoxin-producing Aspergillus flavus (CSCA). In addition, the effect of lactic acid bacterium (LAB) inoculation on the aflatoxin B1 (AFB1) content, genotoxicity, yeast load, and diversity of yeast communities were also investigated. In A. flavus contaminated silages, after two months, the AFB1 content was 40% lower with LAB inoculation, also a lower level of genotoxicity was determined. The number of yeasts cultured from the initial mixture of chopped whole crop corn was 4.8 × 107 CFU g−1 wet mass, while only 2.4 × 106 CFU g−1 from the CSCA and 7.1 × 105 CFU g−1 from the LAB-inoculated CSCA could be cultured. Based on 144 randomly isolated strains, the yeast community of the initial mixture consisted of 8 species. In contrast, the yeast community of CSCA consisted only of 4 species determined by 132 randomly selected isolates. LAB-inoculated CSCA consisted also of 4 species based on 158 randomly isolated strains. Saccharomyces cerevisiae and Pichia kudriavzevii proved to be predominant in the CSCA, while S. cerevisiae and Meyerozyma guilliermondii were the most abundant in the LAB-inoculated CSCA. The species richness was also confirmed by alpha diversity values (1.827, 1.188, and 1.123 as Shannon's indices for CS, CSCA, and LAB-inoculated CSCA, respectively). In response to LAB inoculation, the species diversity decreased considerably.
Abstract
This study aims to predict drought periods affecting the Tokaj-Hegyalja wine region and the application of this in crop protection. The Tokaj-Hegyalja wine region is the only closed wine region in Hungary with a specific mesoclimate and a corresponding wine grape variety composition, in which climate change strongly threatens cultivation. The probability that a randomly selected day in the vegetation period will fall into a drought period in the future was estimated using the daily precipitation amount and daily maximum temperature data from the Hungarian Meteorological Service for the period 2002–2020. The Markov model, a relatively new mathematical method for the statistical investigation of weather phenomena, was used for this. Markov chains can, therefore, be a valuable tool for organizing integrated pest management. This can be used to plan irrigation, control fungal pathogens infecting the vines, and plan the success of a given vintage.
Abstract
Dengue is a serious epidemic for Bangladesh affecting thousands of lives. This study aimed to identify and determine the prevalence of the circulating variants of dengue virus (DENV) and their association with demographics and clinical manifestations among the dengue-infected patients. A total of 711 participants with NS1 antigen positivity were enrolled, followed by viral RNA extraction from the collected blood samples and a multiplex real-time reverse transcription-polymerase chain reaction (RT-PCR) assay to determine the dengue virus serotypes. Of 711 dengue-infected patients, 503 (70.7%) were male. Among different age groups, most of the patients were 21–30 years old (n = 255, 35.9%). The DENV2 (n = 483, 67.9%) serotype was more prevalent than the DENV3 (n = 144, 20.3%) and DENV1 (n = 84, 11.8%). The duration of fever was highest in the DENV-1 patients (4.79 ± 1.84 days) in contrast to DENV-3 (4.48 ± 1.68 days) and DENV-2 (4.33 ± 1.45 days) (P = 0.039). Importantly, five highly populated areas were identified as dengue hotspots in Chittagong metropolitan city. Our results provide crucial insights into the patterns of dengue virus transmission and severity among southern Bangladeshi population, thereby aiding in the development of targeted public health interventions and management strategies to combat future outbreaks.
Abstract
Food manufacturing and processing are part of the nation's critical infrastructure. Due to the recent global spread of the SARS-CoV-2 virus, the potential contamination of the food chain and the resulting public health implications are of high consequence to society. The current primary food manufacturing and processing facilities already have various mechanisms such as hazard analysis and critical control point (HACCP) system in place. However, the widespread microbial infections in these facilities raise concerns that they will not only threaten the welfare of food processing workers, but also have a potentially greater consequence on the public if the food is contaminated with an infectious agent.
Despite the increasingly recognised role of the environment in the spread of microbes, the effect of air properties remains poorly understood. Heating, ventilation, and air conditioning (HVAC) systems in meat processing facilities not only provide a means of transport for viruses and bacteria but may also deposit them on surfaces where they can survive for days. To maintain a stable and safe food chain supply during the pandemic, the challenges to ensure safe food supply and protect the workers' health must be quickly addressed through sustainable, safe and economic approaches. With these two imminent challenges in mind, the overall goal of this review article is to provide a comprehensive overview of the role of the environment in the impaction and resuspension of bioaerosols, focusing on airborne bacteria and viruses. The review includes the latest results of modeling the spread of microbial aerosols in the airflow and the development of preventive measures to mitigate virus contamination in the unique environment of meat processing operations. By understanding how the environmental factors and seasonality affect the infectivity and spread of airborne pathogens, mitigation measures can be designed to minimise future infections within and beyond these facilities.
Abstract
The effect of the storage conditions of light (presence; absence) and relative humidity (50%; 75% RH) on the water content and colour of Porphyridium cruentum microalga powder was studied, then two packaging materials, low-density polyethylene (PE-LD) and metallised polyethylene terephthalate with polyethylene (PETmet/PE), were used to study their effect on the water content, colour, total carotenoids and chlorophylls contents, total phenolic content (TPC), and antioxidant activity (ABTS, DPPH, ORAC) of P. cruentum during storage at 23 °C and 50% RH. An atmosphere with 50% RH is recommended to minimise the water vapour absorption and lightness loss. In addition to the expected light protection, PETmet/PE material protected P. cruentum powder also against water absorption, contrary to PE material. There were no relevant colour changes of the microalga during storage in PETmet/PE. Regarding the total content of carotenoids and the antioxidant activity of the microalga, no significant differences were found between the biomasses stored in either packaging material. The TPC and DPPH values were practically constant during two months of storage, while ABTS and ORAC decreased more than 50% and 20%, respectively, during this time.
Abstract
The utilization of sea buckthorn pomace (SBP) is attracting growing attention since it is valuable industrial waste. This pomace can find usage as a functional ingredient of food because it contains bioactive, health-promoting components, but to our knowledge, few scientists have so far studied utilization of the antimicrobial activity of fruit pomace. The study aims are to broaden our knowledge of antioxidant and antimicrobial status of SBP by utilizing pomace as a functional apple juice ingredient and by monitoring the antioxidant capacity, the total polyphenol content and microbial changes that occur during the storage of juice samples. Our results of this study highlight that the importance of the utilization of SBP because the results reported here provide further evidence that SBP can contribute to increasing the content of valuable components in apple juice samples and inhibiting the growth of microorganisms during storage.
Abstract
A novel magnetic molecular-imprinted polymer (MMIP) was used to selectively extract folic acid directly from real samples. Folic acid was used as template molecule, Fe3O4/SiO2-3-triethoxysilyl-propyl-acrylamide as functional monomer, azobisisobutyronitrile as initiation, ethylene glycol dimethacrylate as crosslinker agent, and acrylamide as the secondary monomer in a mixed ethanol-water solvent. The effect of different parameters on the extraction efficiency was studied, and the optimum conditions were established as follows: the concentrations of crosslinking and template were fixed at 0.05 and 0.06 g, absorption percentage was 96.5, pH was adjusted to 8, and extraction time was 8 h with a temperature of 25 °C. By examining the effect of pH, we tried to investigate the effect of the amide groups that present in MMIP and its intermolecular hydrogen interaction with folic acid. After optimising the effective parameters in polymer synthesis and adsorption rate, a magnetic imprinting dispersive solid-phase extraction method combined with fluorescence spectrophotometry at λem = 367 nm (MMIP-DSPE-FL) was constructed for sensitive determination of folic acid in tomato samples. The limit of quantification (LOQ) and limit of detection (LOD) values were 30.00 ± 0.01 μg L−1 and 10.00 ± 0.03 μg L−1, respectively, after the MMIP-DSPE preconcentration. Three tomato samples were analysed to give recoveries in the range of 80.2–81.6%, with relative standard deviation values below 0.6% (n = 3). The prepared MMIP-DSPE showed high selectivity toward folic acid, which could be used six times without changing adsorption capacity. The adsorption isotherm of the folic acid-imprinted polymer pursued the Langmuir model (RL = 0.029), and the kinetics model followed pseudo-first-order (R 2 = 0.9974).
Abstract
All described Uropodina species (351) are listed from the last 30 years with the original occurrences. On continent level, the most of the described species are from Asia (121), from Central and South America (87) and from Africa (79). On country level, the most species were described from Ecuador (21), Vietnam (21), Kenya (18), Thailand (16), Costa Rica (15), China (14), Slovakia (14), Malaysia (14), Madagascar (14), Brazil (13), Indonesia (11), India (10) and Peru (10) and nine or fewer species were described from the other countries in the last 30 years. Trichouropoda pocsi Kontschán, 2004 (Comoro Islands) is a junior homonymous name of Trichouropoda pocsi Hirschmann & Wisniewski, 1987. The new replacement name for the T. pocsi Kontschán, 2004 is Trichouropoda pocstamasi nom. nov.
Abstract
The work was carried out to explore whether the anti-inflammatory effect of n-3 unsaturated fatty acids on patients with rheumatoid arthritis is related to the levels of inflammatory markers CRP and ESR. Studies on the treatment of rheumatoid arthritis with n-3 unsaturated fatty acid diet therapy and the outcome index containing CRP and/or ESR were included, and studies on the subjects suffering from other diseases affecting the outcome index were excluded. PubMed, Web of Science, EMBASE and Google Scholar were systematically searched, and all studies published from the establishment of the library to 2024 were collected. The Cochrane Bias Risk Assessment tool was used to evaluate the quality of the included studies. Data analysis was performed using Stata 16.0 software. Seven studies were included in this analysis. A total of 399 subjects were studied to explore the effect of an n-3 unsaturated fatty acid diet on rheumatoid arthritis. The results showed that there was no significant difference in CRP (Hedges's g = 0.06, 95% CI: −0.48–0.37, P = 0.79) and ESR (Hedges's g = −0.14, 95% CI: −0.61–0.33, P = 0.55) between the intervention and control groups. The results of this study showed that the anti-inflammatory effect of unsaturated fatty acids on rheumatoid arthritis was not correlated with CRP and ESR levels. Due to the small number of included studies, more high-quality studies are still needed to confirm this.
Abstract
Along with climate change, or by transportation of agricultural products, such as fruits and ornamental plants, their alien insect pests have also started to appear in Hungary. Although these pest species are sometimes sparsely studied, their biology and interactions with their host plants provide key information for monitoring their spread and identifying their vulnerable points, targets for developing methods preventing their overpopulation. In this review article we have collected data on newly emerged moth species recently found in Hungary that might threaten plantations in Hungary in the near future.
Abstract
Foodborne pathogens' transmission is essential in the spread of antibiotic resistance, and extended-spectrum beta-lactamase-producing Escherichia coli especially threatens public health. E. coli plays an essential role in the resistance to commonly used beta-lactam group antibiotics. Ready-to-eat (RTE) stuffed mussels are among many restaurants and street vendors, presenting potential health risks of food hygiene origin. 200 RTE stuffed mussels were collected from the Asian and European sides of Istanbul and analysed for the presence of E. coli. As a result of PCR analysis, E. coli was detected in 7 (3.5%) samples. An antibiotic susceptibility test was performed using the disc diffusion method to determine ESBL and carbapenem resistance. All isolates were resistant to ampicillin. The double-disk synergy test was performed as an ESBL phenotypic confirmation test, and no phenotypically ESBL-producing E. coli were detected. The bla TEM gene was detected in one isolate (14.2%) by mPCR, but bla CTX-M, bla SHV, and bla OXA genes were not observed. Meropenem and imipenem were used with the disk diffusion method for carbapenem resistance study, and no resistant isolate was found. Carbapenem resistance genes were investigated by monoplex PCR, and bla NDM-1, bla OXA-48, bla VIM, and bla IMP resistance genes were not detected. This is the first report on ESBL-producing E. coli in RTE stuffed mussels in Türkiye, which draws attention to a public health risk.
A hazai láptalajok osztályozás-története és az osztályozás továbbfejlesztése
History of classification of bog soils in Hungary and a proposal for the further development of classification
A vitaalapnak szánt dolgozat bemutatja a hazai láptalajok kutatás- és osztályozás történeti (kronológiai) fejlődését 1859-től napjainkig. A bemutató ismertetés, kritikai elemzés kiemeli az osztályozás fejlődése, és a genetikus, talajföldrajzi osztályozás kialakulása szempontjából fontos eredményeket. Meghatározza a kialakult osztályozás továbbfejlesztését képező ismereteket, amelyek a következők (DÖMSÖDI, 2015; DÖMSÖDI, 1988):
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A „láp” és a „láptalaj” képződés genezise: folyamata, szakaszai, típusai
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A láptalajok talajképző tényezői:
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2.1 Az egykori láp környezetének éghajlati viszonyai (főként éghajlati, klimatikus tényező).
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2.2 A láp élővilág jellege: rétláp, mohaláp, al-láp, fel-láp, (ökológiai fejlődéstörténeti) és növényzete (vegetáció) tényező.
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2.3 A láp felszínalaktani jellege és geomorfológiai helye (síkláp, dombláp, völgyláp, medenceláp), domborzati tényező.
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2.4 A láp, illetve a talaj kora (földtörténeti; abszolút, relatív kortényező).
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2.5 A láp, illetve a talaj földhasználata: rét, legelő, erdő, szántó művelése (antropogén hatások tényezője).
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A láptalajok típusai, altípusai, az altípusok típusszelvényei, mindezek definíciói és dimenziói.
Abstract
This study examined the production of kombucha beverages with several combination of black tea and Aloysia citrodora. For this purpose, five types of kombucha drinks were prepared using different ratios of pure black tea and pure A. citrodora (80:0, 50:30, 40:40, 30:50, and 0:80). Total phenolic and flavonoid content, radical-scavenging capacity based on DPPH, ferric reducing antioxidant power (FRAP) in the fermentation period (days 0, 5, 10, and 15), and cytotoxicity on the 15th day have been evaluated. The results showed that phenolic and flavonoid contents and antioxidant properties increased during fermentation (P < 0.01). Using A. citrodora as a substrate enhanced the phenolic and antioxidant properties. The maximum amounts of polyphenols and flavonoids were 240.24 mg GAE/mL and 49.65 μg QE/mL, respectively, and the DPPH value was 92.81% for the samples with an equal ratio of black tea and A. citrodora on the 15th day. In addition, this sample had the highest rate (66%) of destroying PC3 prostate cancer cells at a 600 μg dL−1 concentration. Using A. citrodora in kombucha drink not only improved the health indices but also caused better acceptance by the sensory evaluators compared to the sample of kombucha beverage produced with pure black tea.
Abstract
Our study aims to examine the perceptions of chefs and restaurants' management in Hungary regarding the importance of health and nutrition in menu planning in today's catering businesses. We assessed 190 complete responses from chefs/cooks and managers/owners of restaurants and catering businesses in Hungary between February 2021 and February 2022 via email on four aspects; health, nutrition, nutrition practice, and consumers' concerns. This study shows an overall improvement in chefs' and restaurants' management's nutritional knowledge compared with similar studies in the past. However, most chefs still believe that the customers ignore the nutrition and nutrient content of the meals when dining away from home. In addition, experienced chefs do not perceive that the preparation of low-fat foods requires additional work and that low fat-foods can taste great. This study found no significant differences related to nutrition in menu planning based on chefs' and managers' backgrounds (i.e., gender, age, position, educational level, work experience, and type and location of establishment).
Abstract
This study aimed to evaluate the efficiency of various mash acidification techniques in alcoholic fermentation for the production of apple spirit. Lachancea thermotolerans and Lactiplantibacillus plantarum strains were selected to conduct pre-fermentative acidification of the mash alongside a conventional chemical acidification approach. The results confirm that L. thermotolerans and L. plantarum possess acidifying potential and can serve as effective biotools for the protection of apple mash during fermentation. Through their outstanding lactic acid production (1.05–1.26 g L−1), they were able to reduce the pH of the mash by 0.29–0.40 pH units. Moreover, the sequential fermentation of these strains with Saccharomyces cerevisiae is a powerful strategy to modulate the aroma profile of apple spirits. Spirits were characterised by an enhanced ester profile with particularly increased ethyl lactate concentrations.
Az erdőállományok talajvíz utánpótlódásra gyakorolt hatásának vizsgálata kecskemét-ménteleki mintaterületen
Investigation of the impact of forest stands on groundwater recharge in the Kecskemét-Méntelek study area
Az Alföldet, azon belül is különösen a Homokhátságot érintő talajvízszint süllyedés, súlyos ökológiai és gazdasági következményekkel is járó problémakör. A jelenség hátterében álló lehetséges okokkal kapcsolatban több évtizede zajlik kutatómunka, ugyanakkor ezek relatív súlyának meghatározása a mai napig tudományos vita tárgyát képezi.
Több szerző is kiemeli az erdőtelepítések talajvízszint csökkentő hatásának fontosságát. Ez a hatás két módon, a vegetáció vízfelvétele, illetve a csapadékból történő utánpótlódás csökkentése (intercepció, talajnedvesség felvétel) által jelentkezhet. Ezen mechanizmusok működését vizsgáltuk meg egy akác (Robinia pseudoacacia) és egy fekete fenyő (Pinus nigra) állomány esetében a Homokhátságon, Kecskemét-Ménteleken kialakított mintaterületünkön, 90, 150 és 200 cm-es mélységben, nagy időbeli felbontással mért talajnedvesség, illetve talajvíz adatokra alapozva.
Az adatok alapján feltételezhető, hogy a talajvízből nem történik közvetlen, vagy közvetett vízfelvétel, aminek oka vélhetően a gyökérzóna és a talajvíz közti igen jelentős horizontális távolság. A talajnedvesség esetében a sekélyebb rétegekben egyértelműen jelentkezik az erdőállományok szezonális szárító hatása. Ugyanakkor a mélyebb rétegek talajnedvesség adatai alapján kijelenthető, hogy a csapadékból történő talajvíz visszatöltődésre leginkább az akác állomány alatt van elméleti lehetőség. A látszólagos ellentmondás feltételezhetően a gyökérzet által kialakított makropórusok hatásával magyarázható. Ezt támasztja alá az akác és fekete fenyő állományok közti igen jelentős eltérés is.
Következtetésünk, hogy az erdőállományok lokálisan jelentősen eltérő hatást gyakorolhatnak a talajvízszintre. Ezért a lezajló folyamatok hátterét, általános jellegű megállapítások helyett, az adott hidrológiai rendszer több elemét vizsgáló monitoring adataira alapozva lehetséges csak felderíteni.
Abstract
A new vagrant species of phyllocoptine mites, Paraepitrimerus abaujensis n. sp. associated with Minuartia frutescens (Caryophyllaceae) is described and illustrated from Hungary.
Abstract
This study investigates sustainable methods for producing protein from soybean expeller via pH-shifting processes, aiming to reduce water usage in alkaline extraction by adjusting solid-to-liquid ratios per cycle and employing isoelectric precipitants like lactic acid and lactic acid bacteria (Lactiplantibacillus plantarum and Lactococcus Lactis) to enhance functional and antioxidant properties over a wide pH range. Results indicate that the most efficient approach involves three 1:10 (w/v) extraction cycles with lactic acid bacteria as precipitants, demonstrating high productivity and low specific water consumption. Protein content and recovery yield showed no significant differences compared to alternatives with higher water consumption or less eco-friendly precipitants. Despite lower solubility, protein products precipitated with lactic acid bacteria formed stable emulsions, exhibiting superior free radical scavenging activity.
Abstract
Changes in RNase activities were investigated in extracts from barley near isogenic lines without or with various powdery mildew resistance genes and were compared to changes in wheat and Brachypodium distachyon leaves after powdery mildew infections. In barley, the compatible interaction with powdery mildew induced the highest increase in RNase activity as measured spectrophotometrically. The incompatible interaction that accompanied with hypersensitive reaction in Mla leaves gave less increase, whereas incompatible interactions in Mlg and mlo barley leaves without visible symptoms gave the least increase of RNase activity. In wheat, the largest RNase activity was found in leaves infected with the compatible wheat powdery mildew or wheat stem and leaf rusts. RNase activity in B. distachyon was higher than that in healthy wheat and especially barley leaves. The electrophoretic RNase enzyme activity patterns were different in barley, wheat and B. distachyon plants, but showed similar activities as determined spectrophotometrically. Barley genes encoding endonuclease 2 and ribonuclease 3-like protein X3 showed the highest expression in the compatible barley - barley powdery mildew interaction as measured by RT-qPCR. This correlated with RNase activities in leaf extracts suggesting that RNases in barley and wheat may act as susceptibility factors of powdery mildew and rust diseases.
Abstract
The study aims to investigate how the transfer of matrix materials from eroded asbestos cement products induces stress responses in plants. The paper evaluates the exposure and risk factors of plants, water, and soil to asbestos cement materials. Additionally, the experimental results provide empirical evidence for plant stress responses based on physiological and germination parameters. Contamination of irrigation water with asbestos cement raises environmental concerns due to its potential toxicity to plants and soil quality. Asbestos in irrigation water can lead to toxic stress for plants, affecting germination processes and growth. The paper analyzes the effects of preset doses of irrigation water containing asbestos cement matrix on the germination process and physiological parameters of Solanum lycopersicum in a controlled experiment setting. This research proposes methodological developments that could be valuable for environmental plant protection professionals.
Abstract
Microencapsulation of flaxseed oil (FO) has received lots of attention in the food and biopharmaceutical industries. To produce FO microcapsules, aqueous emulsions of FO with polymeric carbohydrates (maltodextrin (MD) with dextrose equivalent (DE) 19, gum Arabic (GA) and modified starch (MS)) were prepared by a rotor stator homogenization and subsequently, dehydration of emulsions were performed by spray drying (SD). The objective of this research was to study the effects of different combinations of polymeric carbohydrates with FO in emulsion to obtain maximum encapsulation efficiency (EE). A 3 factorials–3 levels Box–Behnken design was used for the optimization purpose. The maximum EE was achieved using 0.79 MD-GA ratio, 20.23% MS and 24.62% FO in emulsion. Microcapsules obtained by optimum condition had EE 77.68%, particle size (D 32) 120.0 ± 0.43 μm, moisture content1.6 ± 0.13%, wettability 192 ± 5.5 s, solubility 75.49 ± 1.3%, bulk density 0.31 ± 0.025 g mL−1, tapped density 0.36 ± 0.01 g mL−1, Carr's Index 13.88 ± 0.01% and Hausner Ratio 1.16 ± 0.01.
Abstract
Cell cultures are models in biological and medical research to understand physiological and pathological processes. Cell lines are not always available depending on cell type and required species. In addition, the immortalization process often affects cell biology. Primary cells generally maintain a greater degree of similarity in short-term culture to the cells in tissue. Goal of this study was to verify the suitability of chicken primary epithelial caecal cells (PECCs) for in vitro investigations of host‒pathogen interactions. Epithelial nature of PECCs was confirmed by detection of tight and adherens junctions and cobblestone-like cell morphology. Sialic acids distribution was similar to that in caecal cyrosections. To understand the capacity of PECCs to respond to microbial challenges, the Toll-like receptors (TLRs) repertoire was determined. Exposure of PECCs to polyinosinic-polycytidylic acid (poly(I:C)) or lipopolysaccharide (LPS) led to upregulation of type I and III interferon (IFN) as well as interleukin (IL-) 1β, IL-6 and IL-8 mRNA expression. Overall, the PECCs showed properties of polarized epithelial cells. The presence of TLRs, their differential expression, as well as pattern recognition receptor dependent immune responses enable PECCs to act as suitable in vitro model for host‒pathogen interaction studies, which are difficult to conduct under in vivo conditions.
This study assesses the elemental composition of Egyptian glauconite sediments, focusing on potentially toxic elements (PTEs) and macronutrients. The primary aim is to evaluate the feasibility of utilizing these sediments as a natural source of potassium for agricultural purposes, besides conventional chemical fertilizers like potassium sulfate. To quantify elemental content, chemical analysis was employed across five distinct grain size fractions after grinding glauconite rock. The assessment included potassium, calcium, sodium, and PTE concentrations, utilizing potassium chloride (KCl) and ammonium acetate lactate (AL Solution) as single extractants, and the BCR extraction protocol, in addition to measuring the pseudo-total content of these elements. Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES) facilitated a comparative analysis of elemental concentrations. Results indicate PTE concentrations within European Union regulations, with an absence of cadmium. Glauconite samples contain approximately 3–3.3% potassium by weight, alongside significant amounts of essential macronutrients (calcium, magnesium) and micronutrients (copper, nickel, zinc) crucial for agriculture. BCR sequential extraction protocol results closely align with or slightly surpass pseudo-total content results. Notably, the AL Solution demonstrates high efficiency compared to KCl or acetic acid in the first step of the BCR method. BCR sequential protocol provides valuable insights into various elemental forms and potential mobility. Overall, this study reveals that glauconite has the potential to serve as a promising alternative potassium fertilizer without causing adverse environmental impacts.
Abstract
Elderberry pomace, a by-product generated by elderberry processing industries, may be a favourable resource for further utilisation due to its components of high nutritional quality. In our research, elderberry pomace extract (EP) was added to apple juice as natural food additive for controlling microbial spoilage and enriching antioxidant components.
During the 8-week storage period of enriched apple juice (EPA) and control apple juice samples antioxidant properties were evaluated using the FRAP assay, Folin–Ciocalteu method, and pH differentiation method. The amount of polyphenols components was quantified using an RP-HPLC method. The microbiological status of samples was studied by determining the total viable and yeast/mould counts. The EPA is an important source of polyphenol components and other bioactive compounds, and the results suggest that extract of elderberry pomace could be a promising natural preservative to improve microbiological stability during refrigerated storage and increase the quality of apple juice.
Abstract
Myricetin has been reported to have a wide variety of beneficial physiological functions. The present study investigated the antihyperlipidaemic activity of myricetin against hyperlipidaemia of high-fat diet-fed obese rats. The four-week antihyperlipidaemic activity was assayed by giving different doses of myricetin to hyperlipidaemic rats. Results showed that myricetin could reduce the harm caused by oxidative stress, decrease thiobarbituric acid reactive substances value, and decrease total cholesterol and triacylglycerol levels of hyperlipidaemic rats. Quantitative analysis of gene expression showed that myricetin's lipid-lowering activity can be activated by downregulating gene expression of fatty acid synthase (FAS) and peroxisome proliferator-activated receptor-γ (PPARγ) with upregulation of hormone-sensitive lipase (HSL) mRNA level. Thus, myricetin had significant health benefits and could be explored as a potentially promising dietary supplement for treating hyperlipidaemia.
Abstract
Botrytis cinerea is one of the fungal pathogens with the widest host plant spectrum, causing serious yield losses and significant economic damage in vineyards from year to year. As an ubiquitous, polyphagous fungal pathogen, with both saprophytic and parasitic lifestyle. The sequential use of active substances belonging to the same chemical family to protect vineyards can lead to an increase in fungal chemical resistance, which is reflected in the enrichment of point mutations in the genomic regions coding proteins involved in the mechanism of action of different pesticides. The aim of our studies was to compare the sensitivity to different fungicides of B. cinerea populations in two wine regions with different pest management practices: the Tokaj region, where the presence of B. cinerea is necessary to produce noble rot wines, and the adjacent Eger Region, where a total protection against B. cinerea is desired. Our study is the first Hungarian report of some previously studied resistance mutations in ERG27 and SDHB protein-coding genes. We identified point mutations in ERG27 transmembrane domain that have not been previously described but may affect the emergence of resistance to certain fungicides. Our study shows that the B. cinerea population of the Northern Hungary region is consistently characterized by an increase in fenhexamid resistance.
Abstract
Conversion of economic microcrystalline cellulose (MCC) into high value-added prebiotic glucans, is not only stimulates utilisation of renewable lignocellulosic biomass, but also provides cheap prebiotics to reduce high incidence of obesity and metabolic syndrome. Herein, glucans (C0.25–C0.50–C1.00) from MCC were prepared by pre-impregnation with dilute sulphuric acid (0.25–0.50–1.00%) and ball-milling treatment for 1 h. NMR spectroscopy and gel-permeation chromatography of the glucan products showed a significant reduction in the degree of polymerisation (DP) and molecular weights (Mw). All prepared glucans improved gut stress evaluated by in vitro digestion and fermentation (young and aging mouse faecal inocula). C1.00 with lower DP and Mw showed better water solubility, earlier peak, and exhibited increased 1-diphenyl-2-picrylhydrazyl activity, higher ratios of Lactobacillus to Escherichia coli, and a higher level of short chain fatty acids better than C0.25 and C0.50 treatment (P < 0.05). Better prebiotic effects were observed in aging mice than in young mice. The highest ratio of Lactobacillus to E. coli was a 2.13-fold increase for aging mice compared to a 1.79-fold increase for young mice, relative to the initial value after C1.00 treatment. The study provides a novel pathway and a new resource for producing glucan.
Abstract
Mango fruits' quality, marketability, and shelf life are significantly influenced by postharvest decay. Mango fruit disease development during storage is inhibited by fungicides. The objective of this investigation was to assess the relative effectiveness of two fungicides, azoxystrobin 18.2% + difenoconazole 11.4% SC and hexaconazole 5% SC, in mitigating postharvest deterioration of mango cv. ‘Langra’ during low temperature storage. Three pathogens were found associated with postharvest decay of mango, i.e. Colletotrichum gloeosporioides, Alternaria alternata, and Penicillium expansum. In comparison to untreated ones, the preharvest application of fungicide treatments provided noticeably better disease control. The foliar application of azoxystrobin 18.2% + difenoconazole 11.4% SC (0.1%) 15 days before the harvesting of fruits significantly improved fruit firmness, soluble solid content, titratable acidity, reduced the disease incidence, percentage disease index, and lesion diameter followed by the application of hexaconazole 5% SC (0.1%) compared to the control. Furthermore, it was found that pre-harvest fungicide spraying of azoxystrobin 18.2% + difenoconazole 11.4% SC (0.1%) was an effective treatment for lowering postharvest deterioration and enhancing the physio-chemical characteristics of mango fruits during low-temperature storage.
Abstract
Ergosterol, as a precursor for synthesising useful molecules like vitamin D2, possesses significant physiological functions in both fungal and human systems. In fungi, ergosterol plays a crucial role in stress responses. In contrast to Saccharomyces cerevisiae yeast, the changes in specific ergosterol content of Kluyveromyces marxianus under various stress conditions are less known. This study investigated how ergosterol content changes in response to different stress factors. Carbon to nitrogen (C/N) ratio was examined using experimental design. The effects of aeration and shear force beside constant overall volumetric mass transfer coefficient (KLa) were examined. Cell growth and specific ergosterol content were investigated using ethanol stress during a two-stage fermentation. Based on the results, contradictory settings regarding C/N ratio and shear force were found to be favourable for cell growth and specific ergosterol content. However, increased aeration consistently elevated specific ergosterol content and favoured cell growth as well (2.5-fold and 1.5-fold, respectively). In K. marxianus fermentations, higher ergosterol yield can be achieved through a two-stage fermentation (138.9 mg L−1 compared to 52.9 mg L−1), where the first stage provides favourable conditions for cell growth, and the second stage involves stress (beneficial for ergosterol production) conditions. Conclusions drawn from the two-stage fermentation results suggest that early transitioning of cell growth to the second phase will not result higher adaption and specific ergosterol content compared to the transition at the end of exponential growth phase.
Abstract
This work proposes the optimisation of the ultrasound assisted extraction (UAE) for total phenolic content (TPC) from potato peels (PP). The optimised extract was obtained using ethanol 20% (v/v) for 30 min at 40 °C, and it was found to be rich in total phenolics (45.03 ± 4.16 mg GAE/g DM) and flavonoids (7.52 EQ/g DM) and exerted a good antioxidant effect with IC50s of 125.42 ± 2.78, 87.21 ± 7.72, and 200.77 ± 13.38 μg mL−1 for DPPH, phosphomolybdate, and FRAP, respectively. PP were used for the fresh cheese formulation, and this supplementation did not impact its physicochemical properties; however, the phenol content and antioxidant capacity of formulated cheeses were improved. PP-enriched fresh cheese presented a good acceptability, even better than the control.
Abstract
Serious risks to human health are posed by acute campylobacteriosis, an enteritis syndrome caused by oral infection with the food-borne bacterial enteropathogen Campylobacter jejuni. Since the risk for developing post-infectious autoimmune complications is intertwined with the severity of enteritis, the search of disease-mitigating compounds is highly demanded. Given that benzoic acid is an organic acid with well-studied health-promoting including anti-inflammatory effects we tested in our present study whether the compound might be a therapeutic option to alleviate acute murine campylobacteriosis. Therefore, microbiota-depleted IL-10−/− mice were perorally infected with C. jejuni and received benzoic acid through the drinking water from day 2 until day 6 post-infection. The results revealed that benzoic acid treatment did not affect C. jejuni colonization in the gastrointestinal tract, but alleviated clinical signs of acute campylobacteriosis, particularly diarrheal and wasting symptoms. In addition, benzoic acid mitigated apoptotic cell responses in the colonic epithelia and led to reduced pro-inflammatory immune reactions in intestinal, extra-intestinal, and systemic compartments tested on day 6 post-infection. Hence, our preclinical placebo-controlled intervention trial revealed that benzoic acid constitutes a promising therapeutic option for treating acute campylobacteriosis in an antibiotic-independent fashion and in consequence, also for reducing the risk of post-infectious autoimmune diseases.
Abstract
This study evaluated the effects of Aronia melanocarpa fruit juice (AMFJ) on the liver in a model of high-fat, high-fructose (HFHF) diet-induced metabolic syndrome (MS). Fifty rats were allocated to five groups – Control, MS, MS+AMFJ2.5, MS+AMFJ5, and MS+AMFJ10. The control group was fed a standard diet, while the other groups were provided a HFHF diet. During MS induction, daily oral treatment was performed. Control and MS groups received 10.0 mL kg−1 distilled water, while the other three groups received AMFJ at doses of 2.5, 5.0, and 10.0 mL kg−1, respectively. After 10 weeks, liver samples were collected and inspected histologically as well as immunohistochemically to determine the expression of Bax, Bcl-2, and MAC387. Bax/Bcl-2 ratio was calculated. In MS rats, steatotic, inflammatory and degenerative alterations of the liver were detected, Bax and MAC387 were markedly elevated, while Bcl-2 was non-significantly reduced. The Bax/Bcl-2 ratio significantly increased. The histopathological alterations were prevented by the AMFJ treatment. Compared to MS group, Bax and MAC387 values were significantly lower and Bcl-2 value was higher resulting in significantly lower (P < 0.001) Bax/Bcl-2 ratio in all AMFJ-treated groups. AMFJ, administered during MS induction in rats, prevented the occurrence of inflammatory, steatotic, degenerative, and pro-apoptotic changes in the liver.
Abstract
The article evaluates how well the goals of the European Green Deal are justified, especially considering the risks to energy and food security arising from the conflict between Russia and Ukraine. We agree with the objectives of the European Green Agreement as a whole, but whether some of the objectives which feature in the EASAC study can be achieved by 2030 is questionable, and the description of the tools necessary to achieve the objectives is incomplete. Among other things, there is hardly any mention of the role played by precision farming with digitalization, which is a revolutionary change from an ecological and economic point of view, in reducing the use of synthetic inputs, in regenerating the original state of the soil, in reducing GHG emissions, thus in increasing biodiversity, and at the same time in intensifying production, and finally in expanding the application of biotechnology. We examine these areas in our analysis. Some of the objectives of the EASAC study to be achieved by 2030 are subject to debate, and the description of the information and communication conditions necessary to achieve the objectives is incomplete. The IoT (Internet of Things) responds to global and local challenges: it integrates the precision technologies, WSNs (Wireless Sensor Networks), artificial intelligence, mobile field (Smart Small Robots) and remote data loggers (UAVs: Unmanned Air Vehicles and satellites), Big Data, and cloud computing. Consequently, decision support is increasingly developing into unmanned decision making. IoT (Internet of Things) is the basis of “Farm to Fork” and “Lab to Field” monitoring approaches.
This article evaluates the implementation of European Green Agreement objectives in light of energy and food security risks arising from the Russia-Ukraine conflict. While overall support for the agreement exists, the feasibility of certain EASAC study objectives by 2030 is called into question due to insufficient tools specifications. Notably absent is the emphasis on precision farming with digitalization, which is a transformative ecological and economic practice. Our analyses look into its function in reducing synthetic inputs, soil regeneration, GHG emission reduction, biodiversity enhancement, production intensification, and biotechnology development. Debates surround EASAC study objectives for 2030, despite limited information and communication restrictions. The Internet of Things (IoT) arises as a solution, combining precision technology, WSNs (wireless sensor networks), AI (artificial intelligence), smart small robots, UAVs (unmanned aerial vehicles), satellites, big data, and cloud computing. As a result, decision support turns toward unmanned decision-making, with IoT laying the groundwork for “Farm to Fork” and “Lab to Field” monitoring systems.
The use of organic fertilization is declining in Hungary due to the sharp fall of livestock stand since the middle of the 1980s. Most farmers are forced to use solely chemical crop enhancers. A bifactorial small plot experiment was carried out between 10 May 2023 and 19 October 2023 in Keszthely, in order to examine the effects of farmyard manure (M), green manure (GM) and stem residues (SR) on the nutrient uptake and nitrogen utilization efficiencies of maize at equidistantly increasing (0–70–140–210–280 kg N ha–1) nitrogen doses. The relationship between some vegetative traits (dry biomass weight, Leaf Area Index (LAI)) and yield, furthermore leaf relative water content (RWC) was also examined. According to the results, organic fertilizer substitution significantly increased the N content both in whole plant and grain samples of NPK+M and NPK+GM+SR treatments, compared to the chemically fertilized control (NPK). In case of P and K only slight differences were observed. Whole plant K contents of NPK+M were significantly higher than in the other treatments (P = 0.045; P = 0,005), furthermore P contents in grain samples were significantly higher in NPK+M (P = 0.004) and NPK+GM+SR (P = 0.05) than in control. Harvest index (HI [%]) of NPK+M and NPK+GM+SR were 1.06 and 1.05 times higher than in NPK. Depending on the treatment, P0023 maize hybrid absorbed 58.7–74.64% of total N uptake in the grain (HIN%), and the utilization of 1 kg N fertilizer for the extra yield above the yield of the individual control was 0.39–1.38 kg (AREN). Significant positive correlations were observed between dry biomass weight and yield (NPK: r = 0.937, P = 0.019; NPK+M: r = 0.971, P = 0.006; NPK+GM+SR: r = 0.88, P = 0.049), furthermore LAI and yield (NPK: r = 0.9, P = 0.037; NPK+M: r = 0.983, P = 0.003; NPK+GM+SR: r = 0.784, P = 0.117). Highest RWC values – which may be related to better soil aggregate stability – were measured in NPK+GM+SR treatment, therefore there may be a great potential in this treatment among drought conditions. The effect of organic amendments is particularly noticeable with smaller nitrogen doses so they should be used to reduce inorganic fertilizer application and the resulting environmental risks.
Abstract
Steviol glycosides (SGs) are natural sweeteners derived from stevia leaves, which have various applications in food and beverage products. The extraction efficiency of SGs depends on several factors, such as solvent type, solid to solvent ratio, centrifugal force, and temperature. This study aimed to compare the effects of different solvents (ethanol and water) and leaf moisture (dry and fresh) on the extraction efficiency of major steviol glycosides (SGs) of rebaudioside A (Reb A), stevioside (ST), and rebaudioside C (Reb C) in stevia (var. Levent 93) plant. A Box–Behnken design was used to optimise the extraction parameters for each extraction type. The results showed that ethanol was a more effective solvent than water, however, aqueous extraction was more environmentally friendly and low-cost, and could also yield high concentrations of SGs when fresh leaves were used. The major SGs had similar concentrations in ethanolic extraction, but Reb A was twice as high as ST in aqueous extraction. Reb C was influenced by the solid to solvent ratio in both extraction types, but more so in water extraction. Temperature had a positive effect on ST and Reb C in water extraction of fresh leaves, but not in water extraction of dry leaves or ethanolic extraction. The results of this study contribute to the optimisation of SGs extraction from stevia leaves and provide insights for future research on the effects of different solvents and extraction parameters on the quality and purity of SGs.
Abstract
Both onion and shallot are the most common ingredients to use for many recipes. Consuming onion and shallot provide health benefits including antidiabetic effects. However, the benefits of onion and shallot mixture at different ratios have not been studied in terms of total phenolic content, total antioxidant capacity, superoxide dismutase (SOD)-like activity, and antidiabetic effects. Our study revealed that shallot significantly increased total phenolic content in the mixture from 492 ± 32 μg mL−1 (100% v/v onion) to 803 ± 24 μg mL−1 (100% v/v shallot) depending on the ratios of shallot in the mixture. Shallot also significantly enhanced total antioxidant capacity, SOD-like activity, α-amylase inhibition, and α-glucosidase inhibition in the mixture. Shallot juice exhibited the highest percentage inhibition of α-amylase activity (40.51 ± 1.57%) and α-glucosidase activity (89.61 ± 2.85%) in comparison to those of onion juice and the mixture. In addition, significant positive correlation between phenolic compound and different variables was observed (P < 0.05). Phenolic compounds present in onion and shallot are probably responsible for many health benefits including antidiabetic effects.
Abstract
Chinese onion (Allium chinense G. Don) is an edible vegetable as well as a traditional Chinese medicine. It is rich in steroidal saponins and possesses broad health benefits. In this study, the optimal extraction protocol of the total saponins from Chinese onion (ACS) was explored, and the content of the total steroidal saponins in ACS reached 56.62%. Network pharmacology was applied to predict the related signalling pathways and targets between the main phytochemicals in ACS and hypercholesterolaemia. Enrichment analysis showed that ACS might intervene hypercholesterolaemia through the PI3K-Akt signalling pathway. Meanwhile, cholesterol-lowering effects were verified by ACS intervention in high-cholesterol diet-induced hypercholesterolaemia in Kunming mice. Compared with the model group, the TC and LDL-C levels of mice were decreased and the HDL-C level increased significantly after administration of ACS at a dose of 200 mg kg−1 day−1. The body weight gain, liver index, and atherosclerosis index all decreased dramatically. ACS could significantly reduce the fat content in the liver and reduce the number of fat droplets from the haematoxylin and eosin (H&E) staining of mouse liver. The immunohistochemical staining indicated that ACS could up-regulate the expression of PI3K protein in the liver, thus playing an anti-hypercholesterolaemic role. This study indicated that ACS exhibited significant therapeutic and preventive effects on hypercholesterolaemia, and exerted anti-hypercholesterolaemia through the PI3K-Akt signalling pathway.
Abstract
Nutritional problems can be seen in children living separated from their families. This cross-sectional pilot study aimed to evaluate intuitive eating, diet quality, and nutritional status of children living separated from their families. A total of 55 adolescent girls aged 12–18 years participated in the study. Anthropometric measurements, biochemical data, and 24-h dietary recall data were collected. The Intuitive Eating Scale-2 (IES-2) and the Turkish version of the Healthy Lifestyle-Diet (HLD-TR) index were used. Participants were divided into two groups according to their scores on the diet quality index using the 50th percentile. Group 1 consisted of participants with a score of 25 points or less, and Group 2 consisted of participants with a score above 25 points. Waist circumference (72.0 (66–83), 65.0 (60–77)) was significantly higher in Group 1 (P < 0.05). IES–2 score (3.05 (2.85–3.40), 3.30 (3.12–3.90)) and two sub–factors; reliance in hunger and satiety cues (2.80 (1.80–3.40), 4.00 (2.60–4.60)) and body–food choice congruence (2.50 (2.00–3.50), 3.50 (3.00–4.50)) were higher in Group 2 (P < 0.05). After adjustment for body mass index, age, and energy intake, there was a significant positive correlation between IES-2 and HLD-TR scores (P = 0.029). In conclusion, it may be advantageous to resort to strategies that promote intuitive eating to reduce obesity and associated problems in this vulnerable group.
Abstract
Background
Waning immunity and emergence of new variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), highlight the need for further research in vaccine development.
Methods
A recombinant fusion protein containing the receptor-binding domain (RBD) fused to the human IgG1 Fc (RBD-Fc) was produced in CHO-K1 cells. RBD-Fc was emulsified with four adjuvants to evaluate its immunogenicity. The RBD-specific humoral and cellular immune responses were assessed by ELISA. The virus neutralizing potency of the vaccine was investigated using four neutralization methods. Safety was studied in mice and rabbits, and Antibody-Dependent Enhancement (ADE) effects were investigated by flow cytometry.
Results
RBD-Fc emulsified in Alum induced a high titer of anti-RBD antibodies with remarkable efficacy in neutralizing both pseudotyped and live SARS-CoV-2 Delta variant. The neutralization potency dropped significantly in response to the Omicron variant. RBD-Fc induced both TH2 and particularly TH1 immune responses. Histopathologic examinations demonstrated no substantial pathologic changes in different organs. No changes in serum biochemical and hematologic parameters were observed. ADE effect was not observed following immunization with RBD-Fc.
Conclusion
RBD-Fc elicits highly robust neutralizing antibodies and cellular immune responses, with no adverse effects. Therefore, it could be considered a promising and safe subunit vaccine against SARS-CoV-2.
Abstract
Acquired benign tracheoesophageal fistulas and bronchoesophageal fistulas (TEF) are typically associated with granulomatous mediastinal infections, 75% of which are iatrogenic. Candida albicans and Actinomyces are commonly occurring organisms, but are uncommon etiologies of TEF. Normal colonization and the slow growth characteristics of some species of these agents rarely result in infection, mycetoma, and broncholithiasis, and thus, delays in diagnosis and treatment are likely. Few reports describe C. albicans or Actinomyces spp. as the etiology of TEF or broncholithiasis. Herein, we report a case of benign acquired TEF secondary to coinfection of Candida and Actinomyces complicated by the formation of an actinomycetoma and broncholithiasis and a comprehensive literature review to highlight the unique nature of this presentation and offer a diagnostic algorithm for diagnosis and treatment of TEFs. Following a presentation of three months of productive cough, choking sensation, night sweats, and weight loss, a bronchoscopy revealed a fistulous connection between the esophagus and the posterior right middle lobe. Pathology identified a calcified fungus ball and a broncholith secondary to the co-infection of Candida and Actinomyces. This unique presentation of Candida and Actinomyces co-infection and the associated diagnostic algorithm are presented as education and a useful tool for clinicians.
The new species, Mastigopelma latiffiana G. E. Lee, E. Pesiu et X. L. He, discovered in Pen-insular Malaysia, is described and illustrated as a new addition to the genus Mastigopelma Mitt., of the family Lepidoziaceae. The new species is corticolous, growing on a tree branch in a moist montane forest in Cameron Highlands, Pahang, at 1,900 m alt. It is characterised by the leafy shoots arising from stolons, branches, when present, all ventral-intercalary, and terminal branching lacking; asymmetrical leaves with almost straight ventral margins, rounded to truncate leaf apices, entire-margined leaves, thickened cell walls with nodular trigones, and glistening-homogeneous oil bodies (2–4 per cell); and retuse to blunt teethed underleaf apices. This discovery has increased the total number of known species of Mastigopelma Mitt. to five. An identification key to all the species of the genus is presented.