Browse Our Biology and Life Sciences Journals
Biology is a study of living objects and their life processes. It examines all aspects of living organisms such as their occurrence, classification, internal and external structure, nutrition, reproduction, inheritance, etc. The term “biology” is commonly replaced by the terms “life sciences” and “biological sciences.” There are dozens of branches of biology. Some of the major ones include:
Biology and Life Sciences
Abstract
The rice bran waxes have a very interesting alcoholic moiety, a mixture of long-chain aliphatic fatty alcohols, collectively known as policosanol. This study aimed to investigate rice bran policosanol extract's effect on testosterone secretion in vitro and in vivo and explore the primary composition ratio. The proliferation of cells and the levels of testosterone in cultured rat Leydig cells were examined, while male sexual behaviour and serum testosterone levels were detected in hydrocortisone-induced mice. It was found that testosterone secretion in primary Leydig cells could be significantly stimulated by a policosanol extract featuring an octacosanol/triacontanol ratio of 3–5/1, with a ratio of 5/1 being the most optimal. A policosanol extract containing 68.72% octacosanol and 13.65% triacontanol was capable of reversing hydrocortisone-induced Kidney-Yang Deficiency syndrome by enhancing sexual behaviour and the weight of reproductive organs, as well as increasing testosterone levels. Consequently, the rice bran policosanol extract with an octacosanol/triacontanol ratio of 3–5/1 could stimulate the secretion of testosterone.
Abstract
Actinomycetoma, a neglected tropical disease affecting the skin and soft tissues, is primarily caused by filamentous bacteria including Nocardia species. Here, we report a healthy 56-year-old man who has a one-year history of nodular lesions with seropurulent discharge on his right knee. Despite negative initial tissue culture, the sulfur granules that were partially acid-fast and Gram-positive branching filamentous rods were revealed in the tissue section. Repeated investigation identified the rare pathogen Nocardia wallacei, using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16S ribosomal ribonucleic acid (rRNA) gene sequencing. The patient was successfully treated with a six-month course of trimethoprim-sulfamethoxazole.
This report describes a rare case of actinomycetoma due to N. wallacei, highlighting the challenges in diagnosis and the importance of accurate pathogen identification for the successful management of infection. The current literature regarding the causative agent will also be discussed.
Abstract
In this study, changes in the odour of apple slices throughout the drying process were monitored and documented using electronic nose sensors, while moisture levels were measured and recorded using an electronic hygrometer. The initial and final moisture contents of apple slices were determined as 86.81 ± 0.05% and 4.92 ± 0.01%, respectively. During drying, apple slices were monitored with six odour sensors powered by an Arduino microprocessor. As the moisture value of the apple slices decreased during the drying process, the electronic nose data also decreased. In addition, the image changes in the apple slices according to the drying level were taught to the Teachable Machine. In this study, it was observed that the machine was able to detect the drying level of apples with 85%–100% accuracy. When 900 apple samples were used in the test phase, machine learning was able to predict the drying level of apples with 100% accuracy based on the confusion matrix data. PCA analysis highlighted different patterns in the sensor data during drying; PLS analysis showed that the sensor data can accurately predict the colour (L*, a*, b*), diameter, and thickness of apple slices with high correlation coefficients (rCV and rPre > 0.9).
Abstract
This study evaluated the impact of storage temperature on the antimicrobial activity of essential oil emulsions of Thymus vulgaris L. (TEOE) and Laurus nobilis (LEOE) at 0.6% against Staphylococcus aureus (ATCC 25923), Bacillus cereus (ATCC 14579), and Listeria innocua (ATCC 33090) inoculated onto cooked chicken breast to simulate post-cooking contamination. Samples stored under: T3 (regular storage at 3 ± 0.5 °C), T8 (inadequate storage 8 ± 1 °C), and T3A (refrigerated storage with simulated temperature abuse at 25 ± 1 °C). Microbial analysis and sensory evaluations were carried out at 0, 24, 36, 72, and 120 h of storage. The GC-MS analysis showed that carvacrol 31.97% and eucalyptol 41.16% were the main components in TEO and LEO, respectively. Storage temperature influenced the antibacterial effect of EOE. TEOE exhibited greater effectiveness at T8 compared to T3, reducing microbial counts to 1 log CFU g−1. Conversely, LEOE demonstrated superior efficacy under T3 in most instances and received a higher acceptability score. Additionally, at T3A, both emulsions demonstrated comparable effectiveness against all bacterial strains, although a slight reduction in antimicrobial proprieties were noted after simulated temperature abuse at 24 h. Therefore, the optimal combination for preserving cooked chicken meat is TEOE under T8 or LEOE under T3; assuming post-cooking contamination.
Abstract
Prescottella (Rhodococcus) soli is a soil-dwelling organism not previously thought to be pathogenic in humans. We discuss the case of a 78-year-old male renal transplant recipient presenting with respiratory symptoms and multiple pulmonary nodules, found to be pulmonary malakoplakia secondary to infection with Prescottella (Rhodococcus) soli. Treatment was commenced with vancomycin, meropenem and azithromycin for an induction period of two weeks and continued with indefinite oral moxifloxacin and azithromycin with significant clinical improvement. Although rare, Prescottella species, including Prescottella soli, should be considered in the differential diagnosis of pulmonary nodules, particularly in immunocompromised patients. More data is required to inform optimal treatment.
Abstract
Lotus root is a popular vegetable known for its nutritional value and unique taste. However, it can be susceptible to heavy metal contamination, which has raised concerns about its safety for consumption. This study aimed to analyse the extent of heavy metal contamination in lotus roots and assess the potential health risks associated with their consumption. The results showed that all measured heavy metal concentrations were below the maximum allowable limits, with contamination indices for all metals across nine provinces remaining below 1.0, classifying the lotus roots as “safe” for consumption. Non-carcinogenic risk assessment using the THQ showed values below 1.0 for all heavy metals in both adults and children, indicating negligible health risks. The HI was calculated as 0.107 for adults and 0.126 for children, with arsenic contributing significantly to these values at 47.76% for adults and 39.55% for children. The carcinogenic risk assessment revealed Carcinogenic Risk (CR) values of 8.37 × 10−8 for Pb, 2.04 × 10−5 for Cr, and 2.30 × 10−5 for As in adults, and 9.86 × 10−8 for Pb, 2.40 × 10−5 for Cr, and 2.71 × 10−5 for As in children. All CR values were within the acceptable range, indicating no apparent carcinogenic effects from lotus root consumption at current intake levels. However, it was noted that increased consumption could elevate risks associated with As, with limits of 174 and 93 g for adults and children, respectively, to maintain safety.
Abstract
Gluten-free bread (GFB) consisting of amaranth flour, tigernut (chufa tuber) flour, apple powder, carrot powder, and soy protein isolate can have osteoprotective effects. Glucocorticoid-induced osteoporosis (GIO) was chosen as a model of experimental osteoporosis. Experimental studies were carried out on white male Wistar rats (n = 150): Group 1 – healthy animals receiving standard diet (StD); Group 2 – GIO rats receiving StD; Group 3 – GIO animals receiving zoledronic acid (ZA) and StD; Group 4 – GIO rats receiving ZA and GFB; Group 5 – GIO animals receiving GFB. We evaluated the general condition of animals and carried out morphological and biochemical studies. Destructive changes in the spatial structure of the bone tissue in GIO, observed in Group 2, led to the inability of the bone to withstand the functional load. At the same time, isolated treatment with ZA had virtually no effect on the final morphological picture. Including GFB in the diet of experimental animals both alone and with ZA had a noticeable protective effect on conditions triggering GIO.
Abstract
Similar to other regions, in East Central Europe, agriculture is the most vulnerable sector regarding the consequences of climate change through increasing variability in weather conditions, even on the short-term and local scales. Agricultural engineers make decisions; higher education has a crucial role in taking proper actions. Our survey aimed to get up-to-date information on the knowledge and attitude of graduating students completing agriculture-related studies at 6 universities located in Hungary, the Czech Republic, Poland, and Slovakia, with particular attention to weather projections, crop production, and soil protection issues. Analyses revealed adequate lexical knowledge of young engineers in each country. They are familiar with the agro-environmental actualities and future trends, issues to address at the right time, and the repertoire of tools and practices for taking efficient measures. A positive attitude toward proactive actions was proved. We report proof of a high level of preparedness resulting from the proper efficacy of agricultural higher education. The content of the curricula and the teaching methodologies meet well the receptiveness to learning in the V4 countries. Our findings confirm that frequent updating of the content of subjects as well as incorporating practical applications that promote active learning and engagement, are more critical today than ever, and it is possible to achieve a high level of preparedness for the challenges in the agricultural sector.
Abstract
This study analyzed the impact of storing Golden Delicious apples at 3 °C with a relative humidity of 85 ± 5% in two environments, namely a darkroom (D03) and a lightroom (L03), on the fruit quality. The research evaluated weight loss (Δm) and color metrics (a*, b*, L*, ΔE, C*, hue angle). The number of apples used for each storage was 12. Weight loss was calculated by measuring the changes in mass during storage. Color differences (ΔE), chroma (C*), and hue angle were determined based on the L*a*b* scale values. Apples stored at 3 °C in D03 experienced a minimal percentage of weight loss of 7.02%. The results indicated that compared to the D03 storage, the L03 storage had a more significant increase in Δm and color variation. The non-destructive method of measuring color characteristics facilitates the assessment of the storage conditions for Golden Delicious apples.
Abstract
Nutritional information on packaging is becoming increasingly important in the food industry. Currently, labels are seen not only on the back of the packaging but also on the front. As there are many versions of front-of-pack labels (FoPLs), the research aims to determine which helps consumers the most in making decisions about which food to include in a healthier lifestyle. Nutri-Score, Guideline Daily Amount (GDA) and Multiple Traffic Lights (MTL) FoPLs on cereals were compared using eye-tracking (ET) and choice-based conjoint analysis (CBCA). CBCA was used to assess consumer preferences and the labels and products were also ranked. Based on the results, GDA type FoPL proved to be the most useful based on conjoint analysis, ranking and the analysis of ET parameters. This label helped participants the most in choosing the product that best fits into a healthier lifestyle. The Nutri-Score label, which offers little information on a product's nutritional content, was not favourably received by the Hungarian sample, who preferred more detailed FoP labels.
